{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T17:03:26Z","timestamp":1791219806994,"version":"4.1.0"},"reference-count":61,"publisher":"The Company of Biologists","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,3,15]]},"abstract":"<jats:p>Previous research has suggested that the peaks in the first derivative (dEGG) of the electroglottographic (EGG) signal are good approximate indicators of the events of glottal opening and closing. These findings were based on high-speed video (HSV) recordings with frame rates 10 times lower than the sampling frequencies of the corresponding EGG data. The present study attempts to corroborate these previous findings, utilizing super-HSV recordings. The HSV and EGG recordings (sampled at 27 and 44 kHz, respectively) of an excised canine larynx phonation were synchronized by an external TTL signal to within 0.037 ms. Data were analyzed by means of glottovibrograms, digital kymograms, the glottal area waveform and the vocal fold contact length (VFCL), a new parameter representing the time-varying degree of \u2018zippering\u2019 closure along the anterior\u2013posterior (A\u2013P) glottal axis. The temporal offsets between glottal events (depicted in the HSV recordings) and dEGG peaks in the opening and closing phase of glottal vibration ranged from 0.02 to 0.61 ms, amounting to 0.24\u201310.88% of the respective glottal cycle durations. All dEGG double peaks coincided with vibratory A\u2013P phase differences. In two out of the three analyzed video sequences, peaks in the first derivative of the VFCL coincided with dEGG peaks, again co-occurring with A\u2013P phase differences. The findings suggest that dEGG peaks do not always coincide with the events of glottal closure and initial opening. Vocal fold contacting and de-contacting do not occur at infinitesimally small instants of time, but extend over a certain interval, particularly under the influence of A\u2013P phase differences.<\/jats:p>","DOI":"10.1242\/jeb.093203","type":"journal-article","created":{"date-parts":[[2014,3,12]],"date-time":"2014-03-12T21:51:07Z","timestamp":1394661067000},"page":"955-963","source":"Crossref","is-referenced-by-count":55,"title":["Glottal opening and closing events investigated by electroglottography and super-high-speed video recordings"],"prefix":"10.1242","volume":"217","author":[{"given":"Christian T.","family":"Herbst","sequence":"first","affiliation":[{"name":"Voice Research Laboratory, Department of Biophysics, Faculty of Science, Palack\u00fd University Olomouc, tr. 17. 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Am."},{"key":"2021042508120538700_R2","first-page":"119","article-title":"Observation of vocal fold vibration: measurements of excised larynges","volume-title":"Vocal Fold Physiology","author":"Baer","year":"1981"},{"key":"2021042508120538700_R3","doi-asserted-by":"crossref","first-page":"1304","DOI":"10.1121\/1.389279","article-title":"Laryngeal vibrations: a comparison between high-speed filming and glottographic techniques","volume":"73","author":"Baer","year":"1983","journal-title":"J. Acoust. Soc. Am."},{"key":"2021042508120538700_R4","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1016\/S0892-1997(05)80123-7","article-title":"Electroglottography","volume":"6","author":"Baken","year":"1992","journal-title":"J. Voice"},{"key":"2021042508120538700_R5","volume-title":"Clinical Measurement of Speech and Voice","author":"Baken","year":"2000","edition":"2nd edn."},{"key":"2021042508120538700_R6","doi-asserted-by":"crossref","first-page":"871","DOI":"10.1288\/00005537-198707000-00019","article-title":"Laryngeal modeling: theoretical, in vitro, in vivo","volume":"97","author":"Berke","year":"1987","journal-title":"Laryngoscope"},{"key":"2021042508120538700_R7","doi-asserted-by":"crossref","first-page":"3595","DOI":"10.1121\/1.409875","article-title":"Interpretation of biomechanical simulations of normal and chaotic vocal fold oscillations with empirical eigenfunctions","volume":"95","author":"Berry","year":"1994","journal-title":"J. Acoust. Soc. Am."},{"key":"2021042508120538700_R8","first-page":"289","article-title":"Videostroboscopic evaluation of the larynx","volume":"66","author":"Bless","year":"1987","journal-title":"Ear Nose Throat J."},{"key":"2021042508120538700_R9","first-page":"131","article-title":"A critical review of electroglottography","volume":"12","author":"Childers","year":"1985","journal-title":"Crit. Rev. Biomed. Eng."},{"key":"2021042508120538700_R10","first-page":"202","article-title":"Electroglottography, speech, and ultra-high speed cinematography","volume-title":"Vocal Fold Physiology and Biophysics of Voice","author":"Childers","year":"1983"},{"key":"2021042508120538700_R11","doi-asserted-by":"crossref","first-page":"1309","DOI":"10.1121\/1.394382","article-title":"A model for vocal fold vibratory motion, contact area, and the electroglottogram","volume":"80","author":"Childers","year":"1986","journal-title":"J. Acoust. Soc. Am."},{"key":"2021042508120538700_R12","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1097\/MOO.0b013e3283395dd4","article-title":"State of the art laryngeal imaging: research and clinical implications","volume":"18","author":"Deliyski","year":"2010","journal-title":"Curr. Opin. Otolaryngol. Head Neck Surg."},{"key":"2021042508120538700_R13","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1159\/000111802","article-title":"Clinical implementation of laryngeal high-speed videoendoscopy: challenges and evolution","volume":"60","author":"Deliyski","year":"2008","journal-title":"Folia Phoniatr. Logop."},{"key":"2021042508120538700_R14","first-page":"66","article-title":"Un proc\u00e9d\u00e9 \u00e9lectrique percuntan\u00e9 d'inscription de l'accolement glottique au cours de la phonation: glottographie de haute fr\u00e9quence; premiers r\u00e9sultats (A non-invasive electric method for measuring glottal closure during phonation: high frequency glottography; first results)","volume":"141","author":"Fabre","year":"1957","journal-title":"Bull. Acad Natl. Med."},{"key":"2021042508120538700_R15","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1109\/TAU.1968.1161949","article-title":"Self oscillating source for vocal tract synthesizers","volume":"16","author":"Flanagan","year":"1968","journal-title":"IEEE Trans. Audio Electroacoust."},{"key":"2021042508120538700_R16","first-page":"172","article-title":"First applications of a new laryngograph","volume":"21","author":"Fourcin","year":"1971","journal-title":"Med. Biol. Illus."},{"key":"2021042508120538700_R17","first-page":"141","article-title":"Objective comparison of the electroglottogram to synchronous high-speed images of vocal-fold contact during vibration","volume-title":"Proceedings of the 6th International Workshop on Models and Analysis of Vocal Emissions for Biomedical Applications MAVEBA","author":"Golla","year":"2009"},{"key":"2021042508120538700_R18","doi-asserted-by":"crossref","first-page":"1321","DOI":"10.1121\/1.1646401","article-title":"On the use of the derivative of electroglottographic signals for characterization of nonpathological phonation","volume":"115","author":"Henrich","year":"2004","journal-title":"J. Acoust. Soc. Am."},{"key":"2021042508120538700_R19","doi-asserted-by":"crossref","first-page":"1417","DOI":"10.1121\/1.1850031","article-title":"Glottal open quotient in singing: measurements and correlation with laryngeal mechanisms, vocal intensity, and fundamental frequency","volume":"117","author":"Henrich","year":"2005","journal-title":"J. Acoust. Soc. Am."},{"key":"2021042508120538700_R20","unstructured":"Herbst\u2008C. T.\n           (2012). DKG plugin for FIJI. Available at http:\/\/homepage.univie.ac.at\/christian.herbst\/index.php?page=fiji."},{"key":"2021042508120538700_R21","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1080\/14015430500376580","article-title":"A comparison of different methods to measure the EGG contact quotient","volume":"31","author":"Herbst","year":"2006","journal-title":"Logoped. Phoniatr. 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Opin. Otolaryngol. Head Neck Surg."},{"key":"2021042508120538700_R27","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1016\/S0892-1997(00)80034-X","article-title":"Strobophotoglottographic transillumination as a method for the analysis of vocal fold vibration patterns","volume":"14","author":"Hess","year":"2000","journal-title":"J. Voice"},{"key":"2021042508120538700_R28","doi-asserted-by":"crossref","first-page":"1865","DOI":"10.1121\/1.1456517","article-title":"Gender differences in vocal fold contact computed from electroglottographic signals: the influence of measurement criteria","volume":"111","author":"Higgins","year":"2002","journal-title":"J. Acoust. Soc. Am."},{"key":"2021042508120538700_R29","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/S0892-1997(05)80250-4","article-title":"Variation of electrolaryngographically derived closed quotient for trained and untrained adult female singers","volume":"9","author":"Howard","year":"1995","journal-title":"J. Voice"},{"key":"2021042508120538700_R30","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1001\/archotol.130.3.349","article-title":"Variability of electroglottographic glottal closed quotients: necessity of standardization to obtain normative values","volume":"130","author":"Kania","year":"2004","journal-title":"Arch. Otolaryngol. Head Neck Surg."},{"key":"2021042508120538700_R31","doi-asserted-by":"crossref","first-page":"641","DOI":"10.1016\/j.specom.2011.07.010","article-title":"Automatic glottal segmentation using local-based active contours and application to glottovibrography","volume":"54","author":"Karakozoglou","year":"2012","journal-title":"Speech Commun."},{"key":"2021042508120538700_R32","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1016\/j.jvoice.2011.05.005","article-title":"Visualizing phase relations of the vocal folds by means of high-speed videoendoscopy","volume":"26","author":"Krenmayr","year":"2012","journal-title":"J. Voice"},{"key":"2021042508120538700_R33","first-page":"172","article-title":"Effect of subglottal pressure variation on the \u201cclosed quotient\u201d \u2013 comparing data derived from electroglottograms and from flow glottograms","volume-title":"The Voice Foundation's 41st Annual Symposium: Care of the Professional Voice","author":"La","year":"2012"},{"key":"2021042508120538700_R34","doi-asserted-by":"crossref","first-page":"400","DOI":"10.1016\/j.media.2007.04.005","article-title":"Clinically evaluated procedure for the reconstruction of vocal fold vibrations from endoscopic digital high-speed videos","volume":"11","author":"Lohscheller","year":"2007","journal-title":"Med. Image Anal."},{"key":"2021042508120538700_R35","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1109\/TMI.2007.903690","article-title":"Phonovibrography: mapping high-speed movies of vocal fold vibrations into 2-D diagrams for visualizing and analyzing the underlying laryngeal dynamics","volume":"27","author":"Lohscheller","year":"2008","journal-title":"IEEE Trans. Med. Imaging"},{"key":"2021042508120538700_R36","doi-asserted-by":"crossref","first-page":"182","DOI":"10.3109\/14015439.2012.731083","article-title":"Vocal fold vibration amplitude, open quotient, speed quotient and their variability along glottal length: kymographic data from normal subjects","volume":"38","author":"Lohscheller","year":"2013","journal-title":"Log. Phon. Vocol."},{"key":"2021042508120538700_R37","volume-title":"DSP First: a Multimedia Approach","author":"McClellan","year":"1998"},{"key":"2021042508120538700_R38","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1044\/jshd.2702.165","article-title":"Ultra high speed photography in laryngeal physiology","volume":"27","author":"Moore","year":"1962","journal-title":"J. Speech Hear. Disord."},{"key":"2021042508120538700_R39","doi-asserted-by":"crossref","first-page":"1066","DOI":"10.1044\/jshr.3405.1066","article-title":"Assessment of the dynamics of vocal fold contact from the electroglottogram: data from normal male subjects","volume":"34","author":"Orlikoff","year":"1991","journal-title":"J. Speech Hear. Res."},{"key":"2021042508120538700_R40","doi-asserted-by":"crossref","first-page":"816.e13","DOI":"10.1016\/j.jvoice.2012.04.009","article-title":"Analysis of longitudinal phase differences in vocal-fold vibration using synchronous high-speed videoendoscopy and electroglottography","volume":"26","author":"Orlikoff","year":"2012","journal-title":"J. Voice"},{"key":"2021042508120538700_R41","first-page":"5","article-title":"Digital Audio Concepts","volume-title":"The Computer Music Tutorial","author":"Roads","year":"1998"},{"key":"2021042508120538700_R42","first-page":"88","article-title":"Some relations between glottal air flow and vocal fold contact area","volume-title":"Proceedings of the Conference on the Assessment of Vocal Pathology, Vol. ASHA Reports No. 11","author":"Rothenberg","year":"1979"},{"key":"2021042508120538700_R43","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1044\/jshr.3103.338","article-title":"Monitoring vocal fold abduction through vocal fold contact area","volume":"31","author":"Rothenberg","year":"1988","journal-title":"J. Speech Hear. Res."},{"key":"2021042508120538700_R44","doi-asserted-by":"crossref","first-page":"1072","DOI":"10.1177\/000348946006900414","article-title":"Technique of high-speed photography of the larynx","volume":"69","author":"Rubin","year":"1960","journal-title":"Ann. Otol. Rhinol. Laryngol."},{"key":"2021042508120538700_R45","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/S0892-1997(98)80073-8","article-title":"Approximations of open quotient and speed quotient from glottal airflow and EGG waveforms: effects of measurement criteria and sound pressure level","volume":"12","author":"Sapienza","year":"1998","journal-title":"J. Voice"},{"key":"2021042508120538700_R46","first-page":"279","article-title":"Electroglottography and direct measurement of vocal fold contact area","volume-title":"Vocal Fold Physiology: Voice Production, Mechanisms and Functions","author":"Scherer","year":"1988"},{"key":"2021042508120538700_R47","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1016\/S0892-1997(97)80005-7","article-title":"The membranous contact quotient: a new phonatory measure of glottal competence","volume":"11","author":"Scherer","year":"1997","journal-title":"J. Voice"},{"key":"2021042508120538700_R48","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1038\/nmeth.2019","article-title":"Fiji: an open-source platform for biological-image analysis","volume":"9","author":"Schindelin","year":"2012","journal-title":"Nat. Methods"},{"key":"2021042508120538700_R49","article-title":"Measurement of closed quotient in a female singing voice by electroglottography and videokymography","volume-title":"Vth International Conference Advances in Quantitative Laryngology, Groningen, the Netherlands, April 27-28, 2001","author":"Schutte","year":"2001"},{"key":"2021042508120538700_R50","unstructured":"\u0160vec\u2008J. G.\n           (2000). On Vibration Properties of Human Vocal Folds: Voice Registers, Bifurcations, Resonance Characteristics, Development and Application of Videokymography. Doctoral dissertation. Groningen, the Netherlands: University of Groningen."},{"key":"2021042508120538700_R51","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/S0892-1997(96)80047-6","article-title":"Videokymography: high-speed line scanning of vocal fold vibration","volume":"10","author":"\u0160vec","year":"1996","journal-title":"J. Voice"},{"key":"2021042508120538700_R52","doi-asserted-by":"crossref","first-page":"458","DOI":"10.1097\/MOO.0b013e3283581feb","article-title":"Kymographic imaging of laryngeal vibrations","volume":"20","author":"\u0160vec","year":"2012","journal-title":"Curr. Opin. Otolaryngol. Head Neck Surg."},{"key":"2021042508120538700_R53","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1121\/1.2804939","article-title":"Three registers in an untrained female singer analyzed by videokymography, strobolaryngoscopy and sound spectrography","volume":"123","author":"\u0160vec","year":"2008","journal-title":"J. Acoust. Soc. Am."},{"key":"2021042508120538700_R54","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1159\/000263973","article-title":"Analysis of high-speed motion pictures of the vocal folds","volume":"27","author":"Tanabe","year":"1975","journal-title":"Folia Phoniatr. (Basel)"},{"key":"2021042508120538700_R55","first-page":"128","article-title":"The electrolaryngograph as a clinical tool for the observation and analysis of vocal fold vibration","volume-title":"Ninth Symposium Care of the Professional Voice","author":"Teaney","year":"1980"},{"key":"2021042508120538700_R56","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/0167-6393(89)90001-0","article-title":"A four-parameter model of the glottis and vocal fold contact area","volume":"8","author":"Titze","year":"1989","journal-title":"Speech Commun."},{"key":"2021042508120538700_R57","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0892-1997(05)80076-1","article-title":"Interpretation of the electroglottographic signal","volume":"4","author":"Titze","year":"1990","journal-title":"J. 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