{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,19]],"date-time":"2025-09-19T11:15:51Z","timestamp":1758280551555},"reference-count":9,"publisher":"The Company of Biologists","issue":"1","license":[{"start":{"date-parts":[[1983,9,1]],"date-time":"1983-09-01T00:00:00Z","timestamp":431222400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1983,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>In urinary epithelia, like the turtle bladder, protons are transported by a H+-trans locating ATPase located in the luminal membrane. We have recently discovered that the H+ pump is stored in small vesicles that lie underneath the luminal membrane. CO2, a major regulator of H+ transport, causes these vesicles to fuse with the membrane thereby inserting more H+ pumps.<\/jats:p>\n               <jats:p>We have now isolated these vesicles from the turtle bladder and from beef kidney medulla. Based on inhibitor sensitivity and substrate specificity this proton translocating ATPase is different from the mitochondrial F0-F1 ATPase, yeast plasma membrane and the gastric H+,K+-ATPase. Solubilization and reconstitution of the enzyme into liposomes shows retention of transport activity and inhibitor sensitivity.<\/jats:p>","DOI":"10.1242\/jeb.106.1.135","type":"journal-article","created":{"date-parts":[[2021,4,25]],"date-time":"2021-04-25T01:31:28Z","timestamp":1619314288000},"page":"135-141","source":"Crossref","is-referenced-by-count":13,"title":["Regulation of Proton Transport in Urinary Epithelia"],"prefix":"10.1242","volume":"106","author":[{"given":"Qais","family":"Al-Awqati","sequence":"first","affiliation":[{"name":"Columbia University, 630 W 168th St.. Departments of Medicine and Physiology, College of Physicians and Surgeons , New York, NY10032 U.S.A"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen","family":"Gluck","sequence":"additional","affiliation":[{"name":"Columbia University, 630 W 168th St.. Departments of Medicine and Physiology, College of Physicians and Surgeons , New York, NY10032 U.S.A"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Westley","family":"Reeves","sequence":"additional","affiliation":[{"name":"Columbia University, 630 W 168th St.. Departments of Medicine and Physiology, College of Physicians and Surgeons , New York, NY10032 U.S.A"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher","family":"Cannon","sequence":"additional","affiliation":[{"name":"Columbia University, 630 W 168th St.. Departments of Medicine and Physiology, College of Physicians and Surgeons , New York, NY10032 U.S.A"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[1983,9,1]]},"reference":[{"key":"2023051110143207900_JEXBIO_106_1_135C1","first-page":"F502","article-title":"Transport of H+ against electrochemical gradients in the turtle bladder","volume":"233","author":"Al-Awqati","year":"1977","journal-title":"Am. J. Physiol"},{"key":"2023051110143207900_JEXBIO_106_1_135C2","doi-asserted-by":"publisher","first-page":"421","DOI":"10.1085\/jgp.68.4.421","article-title":"Active H+ transport in the turtle urinary bladder: Coupling of transport to glucose oxidation","volume":"68","author":"Beauwens","year":"1976","journal-title":"J. gen. Physiol"},{"key":"2023051110143207900_JEXBIO_106_1_135C3","first-page":"3136","article-title":"Urinary acidification in the turtle bladder is due to a reversible proton translocating ATPase","volume":"76","author":"Dixon","year":"1979","journal-title":"Proc, natn Acad. Sci. U.SA"},{"key":"2023051110143207900_JEXBIO_106_1_135C4","first-page":"1352","article-title":"Renal H+ ATPase is an Fo-Fi ATPase from lysosomal, mitochondrial and chromaffin granule pumps","volume":"42","author":"Gluck","year":"1983","journal-title":"Fedn Proc. Fedn Am. Socs exp. Biol"},{"key":"2023051110143207900_JEXBIO_106_1_135C5","doi-asserted-by":"publisher","first-page":"4327","DOI":"10.1073\/pnas.79.14.4327","article-title":"Exocytosis regulates urinary acidification in turtle bladder by rapid insertion of H+ pumps into the luminal membrane","volume":"79","author":"Gluck","year":"1982","journal-title":"Proc, natn Acad. Sci. U.S.A"},{"key":"2023051110143207900_JEXBIO_106_1_135C6","doi-asserted-by":"publisher","first-page":"9230","DOI":"10.1016\/S0021-9258(18)34052-3","article-title":"The proton translocating ATPase responsible for urinary acidification","volume":"257","author":"Gluck","year":"1982","journal-title":"J, biol. Chem"},{"key":"2023051110143207900_JEXBIO_106_1_135C7","first-page":"232","article-title":"Characteristics of a H+ ATPase from mammalian papilla","volume":"23","author":"Gluck","year":"1983","journal-title":"Kidney Int"},{"key":"2023051110143207900_JEXBIO_106_1_135C8","first-page":"237","article-title":"Role of endocytosis in H+ transport regulation by turtle bladder","volume":"23","author":"Reeves","year":"1983","journal-title":"Kidney Int"},{"key":"2023051110143207900_JEXBIO_106_1_135C9","doi-asserted-by":"publisher","first-page":"890","DOI":"10.1152\/physrev.1974.54.4.890","article-title":"Cellular mechanisms of urinary acidification","volume":"54","author":"Steinmetz","year":"1974","journal-title":"Physiol. Rev"}],"container-title":["Journal of Experimental Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.biologists.com\/jeb\/article-pdf\/106\/1\/135\/2838723\/jexbio_106_1_135.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.biologists.com\/jeb\/article-pdf\/106\/1\/135\/2838723\/jexbio_106_1_135.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,11]],"date-time":"2023-05-11T11:59:39Z","timestamp":1683806379000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.biologists.com\/jeb\/article\/106\/1\/135\/3972\/Regulation-of-Proton-Transport-in-Urinary"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1983,9,1]]},"references-count":9,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1983,9,1]]}},"URL":"https:\/\/doi.org\/10.1242\/jeb.106.1.135","relation":{},"ISSN":["0022-0949","1477-9145"],"issn-type":[{"value":"0022-0949","type":"print"},{"value":"1477-9145","type":"electronic"}],"subject":[],"published":{"date-parts":[[1983,9,1]]}}}