{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T04:50:30Z","timestamp":1780548630794,"version":"3.54.1"},"reference-count":58,"publisher":"The Company of Biologists","issue":"1","license":[{"start":{"date-parts":[[1986,7,1]],"date-time":"1986-07-01T00:00:00Z","timestamp":520560000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1986,7,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Exhaustive exercise induced a severe short-lived (0\u20131 h) respiratory, and longer-lived (0\u20134 h) metabolic, acidosis in the extracellular fluid of the rainbow trout. Blood \u2018lactate\u2019 load exceeded blood \u2018metabolic acid\u2019 load from 1\u201312 h after exercise. Overcompensation occurred, so that by 8\u201312 h, metabolic alkalosis prevailed, but by 24 h, resting acid-base status had been restored. Acid-base changes were similar, and lactate levels identical, in arterial and venous blood. However, at rest venous RBC pHi was significantly higher than arterial (7\u00b742 versus 7\u00b731). After exercise, arterial RBC pHi remained constant, whereas venous RBC pHi fell significantly (to 7\u00b718) but was fully restored by 1 h. Resting mean whole-body pHi, measured by DMO distribution, averaged approx. 7\u00b725 at a pHe of approx. 7\u00b782 and fell after exercise to a low of 6\u00b778 at a pHe of approx. 7\u00b730. Whole-body pHi was slower to recover than pHe, requiring up to 12 h, with no subsequent alkalosis. Whole-body ECFV decreased by about 70 ml kg\u22121 due to a fluid shift into the ICF. Net H+ excretion to the water increased 1 h after exercise accompanied by an elevation in ammonia efflux. At 8\u201312 h, H+ excretion was reduced to resting levels and at 12\u201324 h, a net H+ uptake occurred. Lactate excretion amounted to approx. 1 % of the net H+ excretion and only approx. 2% of the whole blood load. Only a small amount of the anaerobically produced H+ in the ICF appeared in the ECF and subsequently in the water. By 24 h, all the H+ excreted had been taken back up, thus correcting the extracellular alkalosis. The bulk of the H+ load remained intracellular, to be cleared by aerobic metabolism.<\/jats:p>","DOI":"10.1242\/jeb.123.1.93","type":"journal-article","created":{"date-parts":[[2021,4,25]],"date-time":"2021-04-25T09:19:00Z","timestamp":1619342340000},"page":"93-121","source":"Crossref","is-referenced-by-count":128,"title":["Intracellular and Extracellular Acid-Base Status and H+ Exchange with the Environment After Exhaustive Exercise in the Rainbow Trout"],"prefix":"10.1242","volume":"123","author":[{"given":"C. Louise","family":"Milligan","sequence":"first","affiliation":[{"name":"McMaster University Department of Biology , , 1280 Main St West, Hamilton, Ontario, Canada L8S 4K1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chris M.","family":"Wood","sequence":"additional","affiliation":[{"name":"McMaster University Department of Biology , , 1280 Main St West, Hamilton, Ontario, Canada L8S 4K1"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[1986,7,1]]},"reference":[{"key":"2023011316444194200_JEXBIO_123_1_93C1","doi-asserted-by":"publisher","first-page":"223","DOI":"10.1016\/0034-5687(81)90123-7","article-title":"Oxygen transport and acid-base balance in the blood of the sheatfish, Silurus glanis","volume":"46","author":"Albers","year":"1981","journal-title":"Respir. 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