{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T22:26:53Z","timestamp":1767220013268,"version":"3.41.2"},"reference-count":31,"publisher":"The Company of Biologists","license":[{"start":{"date-parts":[[2016,10,15]],"date-time":"2016-10-15T00:00:00Z","timestamp":1476489600000},"content-version":"vor","delay-in-days":288,"URL":"http:\/\/www.biologists.com\/user-licence-1-1"}],"funder":[{"name":"National High Technology Research and Development Program of China","award":["2012AA10A401"],"award-info":[{"award-number":["2012AA10A401"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31302168"],"award-info":[{"award-number":["31302168"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006606","name":"Natural Science Foundation of Tianjin City","doi-asserted-by":"crossref","award":["14JCYBJC30700"],"award-info":[{"award-number":["14JCYBJC30700"]}],"id":[{"id":"10.13039\/501100006606","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Key Laboratory of freshwater aquaculture germplasm resources of Ministry of Agriculture: National Key Technology Program","award":["2012BAD26B00"],"award-info":[{"award-number":["2012BAD26B00"]}]},{"name":"Science and PhD Start-up Fund of Tianjin Normal University","award":["52XB1303"],"award-info":[{"award-number":["52XB1303"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,1,1]]},"abstract":"<jats:p>Crustacean hyperglycemic hormone (CHH) is a neurohormone found only in arthropods that plays a pivotal role in the regulation of hemolymph glucose levels, molting, and stress responses. Although it was determined that a membrane guanylyl cyclase (GC) acts as the CHH receptor in the Y-organ during ecdysteroidogenesis, the identity of the CHH receptor in the hepatopancreas has not been established. In this study, we identified a new molecular, CHH binding protein (CHHBP), as a potential receptor by screening the annotated unigenes from the transcriptome of Eriocheir sinensis, after removal of eyestalk. Analysis of the binding affinity between CHH and CHHBP provided direct evidence that CHH interacts with CHHBP in a specific binding mode. Subsequent analysis showed that CHHBP was expressed primarily in the hepatopancreas and localized on cell membrane. In addition, real-time PCR analysis showed that CHHBP transcript levels gradually increased in the hepatopancreas following eyestalk ablation. RNAi-mediated suppression of CHHBP expression resulted in decreased glucose levels. Furthermore, the reduction of blood glucose induced by CHHBP RNAi reached the same degree as that observed in the eyestalk ablation group, suggesting that CHHBP contributes to glucose metabolism regulated by CHH. Besides, compared to the control group, injection of CHH was unable to rescue the decreased glucose levels in CHHBP RNAi crabs. CHH induced transport of 2-NBDG to the outside of cells, with indispensable assist from CHHBP. Taken together, these findings imply that CHHBP probably acts as one type of the primary signal processor of CHH-mediated regulation of cellular glucose metabolism.<\/jats:p>","DOI":"10.1242\/jeb.133181","type":"journal-article","created":{"date-parts":[[2016,2,20]],"date-time":"2016-02-20T02:34:30Z","timestamp":1455935670000},"source":"Crossref","is-referenced-by-count":10,"title":["CHH binding protein (CHHBP): a newly identified receptor of crustacean hyperglycemic hormone (CHH)"],"prefix":"10.1242","author":[{"given":"Ran","family":"Li","sequence":"first","affiliation":[{"name":"Tianjin Key Laboratory of Animal and Plant Resistance, College of Life Science, Tianjin Normal University, Tianjin, People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jin-Ze","family":"Tian","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory of Animal and Plant Resistance, College of Life Science, Tianjin Normal University, Tianjin, People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cui-Heng","family":"Zhuang","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory of Animal and Plant Resistance, College of Life Science, Tianjin Normal University, Tianjin, People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi-Chen","family":"Zhang","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory of Animal and Plant Resistance, College of Life Science, Tianjin Normal University, Tianjin, People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xu-Yun","family":"Geng","sequence":"additional","affiliation":[{"name":"Tianjin Center for Control and Prevention of Aquatic Animal Infectious Disease, Tianjin, People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li-Na","family":"Zhu","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory of Animal and Plant Resistance, College of Life Science, Tianjin Normal University, Tianjin, People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jin-Sheng","family":"Sun","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory of Animal and Plant Resistance, College of Life Science, Tianjin Normal University, Tianjin, People's Republic of China"},{"name":"Tianjin Center for Control and Prevention of Aquatic Animal Infectious Disease, Tianjin, People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","reference":[{"key":"2021042510054982000_JEB133181C1","doi-asserted-by":"publisher","first-page":"253","DOI":"10.1023\/B:NEUR.0000010084.10383.3b","article-title":"Morphology and monoaminergic modulation of crustacean hyperglycemic hormone-like immunoreactive neurons in the lobster nervous system","volume":"32","author":"Basu","year":"2003","journal-title":"J. Neurocytol."},{"key":"2021042510054982000_JEB133181C32","doi-asserted-by":"publisher","first-page":"460","DOI":"10.1177\/030098589503200502","article-title":"Glycogen storage disease type Ia in two littermate maltese puppies","volume":"32","author":"Brix","year":"1995","journal-title":"Vet. Pathol."},{"key":"2021042510054982000_JEB133181C2","doi-asserted-by":"publisher","first-page":"214","DOI":"10.1016\/S0016-6480(03)00263-6","article-title":"Crustacean neuropeptide genes of the CHH\/MIH\/GIH family: implications from molecular studies","volume":"134","author":"Chan","year":"2003","journal-title":"Gen. Comp. Endocrinol."},{"key":"2021042510054982000_JEB133181C3","doi-asserted-by":"publisher","first-page":"3280","DOI":"10.1046\/j.1432-1033.2003.03720.x","article-title":"Moult cycle-related changes in biological activity of moult-inhibiting hormone (MIH) and crustacean hyperglycaemic hormone (CHH) in the crab, Carcinus maenas. From target to transcript","volume":"270","author":"Chung","year":"2003","journal-title":"FEBS J."},{"key":"2021042510054982000_JEB133181C4","doi-asserted-by":"publisher","first-page":"206","DOI":"10.1016\/j.ygcen.2006.01.002","article-title":"Binding sites of crustacean hyperglycemic hormone and its second messengers on gills and hindgut of the green shore crab, Carcinus maenas: a possible osmoregulatory role","volume":"147","author":"Chung","year":"2006","journal-title":"Gen. Comp. Endocrinol."},{"key":"2021042510054982000_JEB133181C5","doi-asserted-by":"publisher","first-page":"447","DOI":"10.1016\/j.ygcen.2009.12.011","article-title":"Crustacean hyperglycemic hormone (CHH) neuropeptides family: functions, titer, and binding to target tissues","volume":"166","author":"Chung","year":"2010","journal-title":"Gen. Comp. Endocrinol."},{"key":"2021042510054982000_JEB133181C6","doi-asserted-by":"publisher","first-page":"1705","DOI":"10.1074\/jbc.M707853200","article-title":"Involvement of p90 ribosomal S6 kinase in termination of cell cycle arrest during development of Artemia-encysted embryos","volume":"283","author":"Dai","year":"2008","journal-title":"J. Biol. Chem."},{"key":"2021042510054982000_JEB133181C7","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1016\/j.ygcen.2007.07.001","article-title":"Ecdysteroids, juvenile hormone and insect neuropeptides: recent successes and remaining major challenges","volume":"155","author":"De Loof","year":"2008","journal-title":"Gen. Comp. Endocrinol."},{"key":"2021042510054982000_JEB133181C8","doi-asserted-by":"publisher","first-page":"705","DOI":"10.1096\/fj.05-4661fje","article-title":"Spatial and temporal regulation of GLUT4 translocation by flotillin-1 and caveolin-3 in skeletal muscle cells","volume":"20","author":"Fecchi","year":"2006","journal-title":"FEBS J."},{"key":"2021042510054982000_JEB133181C9","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.pneurobio.2006.07.005","article-title":"A review of neurohormone GPCRs present in the fruitfly Drosophila melanogaster and the honey bee Apis mellifera","volume":"80","author":"Hauser","year":"2006","journal-title":"Prog. Neurobiol."},{"key":"2021042510054982000_JEB133181C10","doi-asserted-by":"publisher","first-page":"142","DOI":"10.1016\/j.yfrne.2007.10.003","article-title":"A genome-wide inventory of neurohormone GPCRs in the red flour beetle Tribolium castaneum","volume":"29","author":"Hauser","year":"2008","journal-title":"Front. Neuroendocrinol."},{"key":"2021042510054982000_JEB133181C11","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/S1673-8527(08)60001-6","article-title":"New players in the regulation of ecdysone biosynthesis","volume":"35","author":"Huang","year":"2008","journal-title":"J. Genet. Genomics"},{"key":"2021042510054982000_JEB133181C12","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1016\/0014-5793(89)81051-8","article-title":"Amino acid sequence of the crustacean hyperglycemic hormone (CHH) from the shore crab, Carcinus maenas","volume":"255","author":"Kegel","year":"1989","journal-title":"FEBS Lett."},{"key":"2021042510054982000_JEB133181C31","doi-asserted-by":"publisher","first-page":"83","DOI":"10.1354\/vp.38-1-83","article-title":"Canine model and genomic structural organization of glycogen storage disease Type Ia (GSD Ia)","volume":"38","author":"Kishnani","year":"2001","journal-title":"Vet. Pathol."},{"key":"2021042510054982000_JEB133181C14","doi-asserted-by":"publisher","first-page":"727","DOI":"10.1002\/jez.a.59","article-title":"Species-specific effects on hemolymph glucose control by serotonin, dopamine, and L-enkephalin and their inhibitors in Squilla mantis and Astacus leptodactylus (crustacea)","volume":"301A","author":"Lorenzon","year":"2004","journal-title":"J. Exp. Zool. A Comp. Exp. Biol."},{"key":"2021042510054982000_JEB133181C15","doi-asserted-by":"publisher","first-page":"3341","DOI":"10.1242\/jeb.01761","article-title":"Role of biogenic amines and cHH in the crustacean hyperglycemic stress response","volume":"208","author":"Lorenzon","year":"2005","journal-title":"J. Exp. Biol."},{"key":"2021042510054982000_JEB133181C16","doi-asserted-by":"publisher","first-page":"189","DOI":"10.1046\/j.1365-2095.2003.00243.x","article-title":"Utilization of gelatinized carbohydrate in diets of Labeo rohita fry","volume":"9","author":"Mohapatra","year":"2003","journal-title":"Aquac. Nut."},{"key":"2021042510054982000_JEB133181C17","doi-asserted-by":"publisher","first-page":"62","DOI":"10.1186\/1471-2148-10-62","article-title":"Molecular evolution of the crustacean hyperglycemic hormone family in ecdysozoans","volume":"10","author":"Montagn\u00e9","year":"2010","journal-title":"BMC Evol. Biol."},{"key":"2021042510054982000_JEB133181C18","doi-asserted-by":"publisher","first-page":"48834","DOI":"10.1074\/jbc.M209082200","article-title":"Flotillin-1\/reggie-2 traffics to surface raft domains via a novel golgi-independent pathway: identification of a novel membrane targeting domain and a role for palmitoylation","volume":"277","author":"Morrow","year":"2002","journal-title":"J. Biol. Chem."},{"key":"2021042510054982000_JEB133181C19","doi-asserted-by":"publisher","first-page":"293","DOI":"10.1016\/j.ygcen.2011.03.019","article-title":"Effects of crustacean hyperglycemic hormone (CHH) on the transcript expression of carbohydrate metabolism-related enzyme genes in the kuruma prawn, Marsupenaeus japonicus","volume":"172","author":"Nagai","year":"2011","journal-title":"Gen. Comp. Endocrinol."},{"key":"2021042510054982000_JEB133181C20","doi-asserted-by":"publisher","first-page":"32166","DOI":"10.1074\/jbc.M114.590646","article-title":"Identification and characterization of receptors for ion transport peptide (ITP) and ITP-like (ITPL) in the silkworm Bombyx mori","volume":"289","author":"Nagai","year":"2014","journal-title":"J. Biol. Chem."},{"key":"2021042510054982000_JEB133181C21","doi-asserted-by":"publisher","first-page":"17","DOI":"10.2307\/1543521","article-title":"Methionine-enkephalin induces hyperglycemia through eyestalk hormones in the estuarine crab Scylla serrata","volume":"201","author":"Reddy","year":"2001","journal-title":"Biol. Bull."},{"key":"2021042510054982000_JEB133181C22","doi-asserted-by":"publisher","first-page":"423","DOI":"10.1016\/0300-9629(87)90146-0","article-title":"The role of hepatopancreatic glycogen in the action of the crustacean hyperglycemic hormone (CHH)","volume":"87","author":"Sedlmeier","year":"1987","journal-title":"Comp. Biochem. Physiol. A Physiol."},{"key":"2021042510054982000_JEB133181C23","doi-asserted-by":"publisher","first-page":"41","DOI":"10.1006\/gcen.1996.0092","article-title":"Characterization of hyperglycemic and molt-inhibiting activity from sinus glands of the penaeid shrimp Penaeus vannamei","volume":"103","author":"Sefiani","year":"1996","journal-title":"Gen. Comp. Endocrinol."},{"key":"2021042510054982000_JEB133181C24","doi-asserted-by":"publisher","first-page":"425","DOI":"10.1016\/S0968-0004(99)01467-X","article-title":"The SPFH domain: implicated in regulating targeted protein turnover in stomatins and other membrane-associated proteins","volume":"24","author":"Tavernarakis","year":"1999","journal-title":"Trends Biochem. Sci."},{"key":"2021042510054982000_JEB133181C25","first-page":"36","article-title":"Endocrinology of metabolism and water balance: crustacean hyperglycemic hormone","volume-title":"The Natural History of the Crustacea, Vol. 4, Physiology","author":"Webster","year":"2015"},{"key":"2021042510054982000_JEB133181C26","doi-asserted-by":"publisher","first-page":"217","DOI":"10.1016\/j.ygcen.2011.11.035","article-title":"The CHH-superfamily of multifunctional peptide hormones controlling crustacean metabolism, osmoregulation, moulting, and reproduction","volume":"175","author":"Webster","year":"2012","journal-title":"Gen. Comp. Endocrinol."},{"key":"2021042510054982000_JEB133181C27","doi-asserted-by":"publisher","first-page":"387","DOI":"10.1006\/gcen.1994.1138","article-title":"Characterization of crustacean hyperglycemic hormone from the crayfish (Procambarus clarkii): multiplicity of molecular forms by stereoinversion and diverse functions","volume":"95","author":"Yasuda","year":"1994","journal-title":"Gen. Comp. Endocrinol."},{"key":"2021042510054982000_JEB133181C28","doi-asserted-by":"publisher","first-page":"351","DOI":"10.1016\/j.bbrc.2005.12.186","article-title":"YHV-protease dsRNA inhibits YHV replication in Penaeus monodon and prevents mortality","volume":"341","author":"Yodmuang","year":"2006","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"2021042510054982000_JEB133181C29","doi-asserted-by":"publisher","first-page":"479","DOI":"10.1186\/1743-422X-8-479","article-title":"Research advances on flotillins","volume":"8","author":"Zhao","year":"2011","journal-title":"Virol. J."},{"key":"2021042510054982000_JEB133181C30","doi-asserted-by":"publisher","first-page":"88","DOI":"10.1016\/j.ygcen.2007.04.002","article-title":"Complex steroid-peptide-receptor cascade controls insect ecdysis","volume":"153","author":"\u017dit\u0148an","year":"2007","journal-title":"Gen. Comp. Endocrinol."}],"container-title":["Journal of Experimental Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1242\/jeb.133181","content-type":"unspecified","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/journals.biologists.com\/jeb\/article-pdf\/219\/8\/1259\/1892472\/jeb133181.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/journals.biologists.com\/jeb\/article-pdf\/doi\/10.1242\/jeb.133181\/2039080\/jeb_133181v1.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"http:\/\/journals.biologists.com\/jeb\/article-pdf\/doi\/10.1242\/jeb.133181\/2039080\/jeb_133181v1.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,5,18]],"date-time":"2021-05-18T19:43:25Z","timestamp":1621367005000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.biologists.com\/jeb\/article\/doi\/10.1242\/jeb.133181\/262193\/CHH-binding-protein-CHHBP-a-newly-identified"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,1,1]]},"references-count":31,"URL":"https:\/\/doi.org\/10.1242\/jeb.133181","relation":{},"ISSN":["1477-9145","0022-0949"],"issn-type":[{"type":"electronic","value":"1477-9145"},{"type":"print","value":"0022-0949"}],"subject":[],"published":{"date-parts":[[2016,1,1]]},"article-number":"jeb.133181"}}