{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T06:58:14Z","timestamp":1774940294453,"version":"3.50.1"},"reference-count":30,"publisher":"The Company of Biologists","license":[{"start":{"date-parts":[[2017,3,15]],"date-time":"2017-03-15T00:00:00Z","timestamp":1489536000000},"content-version":"vor","delay-in-days":439,"URL":"http:\/\/www.biologists.com\/user-licence-1-1"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000038","name":"NSERC","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Donald M. Ross Scholarship"},{"name":"R.E. Peter Memorial Scholarship"},{"name":"Andrew Stewart Memorial Prize"},{"name":"Western Canadian Universities Marine Sciences Society"},{"name":"Dick and Leona Peter \/ Bamfield Marine Sciences Centre"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,1,1]]},"abstract":"<jats:p>Hagfish skin has been reported as an important site for ammonia excretion and as the major site of systemic oxygen acquisition. However, debate remains whether cutaneous O2 uptake is the dominant route of uptake; all evidence supporting this hypothesis has been derived using indirect measurements. Here we use separating chambers and direct measurements of oxygen consumption and ammonia excretion to quantify cutaneous and branchial exchanges in Pacific hagfish (Eptatretus stoutii) at rest and following exhaustive exercise. Hagfish primarily relied on the gills for both O2 uptake (81.0%) and ammonia excretion (70.7%). Following exercise, both O2 uptake and ammonia excretion increased, but only across the gill; cutaneous exchange was not increased. When branchial O2 availability was reduced by exposure to anteriorly-localized hypoxia (\u223c4.6 kPa O2), cutaneous O2 consumption was only slightly elevated on an absolute basis. These results refute a major role for cutaneous O2 acquisition in the Pacific hagfish.<\/jats:p>","DOI":"10.1242\/jeb.141598","type":"journal-article","created":{"date-parts":[[2016,7,12]],"date-time":"2016-07-12T05:15:06Z","timestamp":1468300506000},"source":"Crossref","is-referenced-by-count":15,"title":["It's all in the gills: Evaluation of O2 uptake in Pacific hagfish refutes a major respiratory role for the skin"],"prefix":"10.1242","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2836-5832","authenticated-orcid":false,"given":"Alexander M.","family":"Clifford","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, University of Alberta, 116 St. and 85 Ave., Edmonton, Alberta, T6G 2R3, Canada"},{"name":"Bamfield Marine Sciences Centre, 100 Pachena Rd., Bamfield, British Columbia, V0R 1B0, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alex M.","family":"Zimmer","sequence":"additional","affiliation":[{"name":"Bamfield Marine Sciences Centre, 100 Pachena Rd., Bamfield, British Columbia, V0R 1B0, Canada"},{"name":"Department of Zoology, University of British Columbia, Vancouver, British Columbia, V6T 1Z4, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chris M.","family":"Wood","sequence":"additional","affiliation":[{"name":"Bamfield Marine Sciences Centre, 100 Pachena Rd., Bamfield, British Columbia, V0R 1B0, Canada"},{"name":"Department of Zoology, University of British Columbia, Vancouver, British Columbia, V6T 1Z4, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Greg G.","family":"Goss","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, University of Alberta, 116 St. and 85 Ave., Edmonton, Alberta, T6G 2R3, Canada"},{"name":"Bamfield Marine Sciences Centre, 100 Pachena Rd., Bamfield, British Columbia, V0R 1B0, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","reference":[{"key":"2021042510233508700_JEB141598C1","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/S1546-5098(08)60323-4","article-title":"Appendix: Physicochemical parameters for use in fish respiratory physiology","volume-title":"Gills-Anatomy, Gas Transfer, and Acid-Base Regulation","author":"Boutilier","year":"1984"},{"key":"2021042510233508700_JEB141598C2","doi-asserted-by":"publisher","first-page":"82","DOI":"10.1016\/j.cbpa.2013.11.009","article-title":"Extrabranchial mechanisms of systemic pH recovery in hagfish (Eptatretus stoutii)","volume":"168","author":"Clifford","year":"2014","journal-title":"Comp. 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