{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T15:00:31Z","timestamp":1780066831218,"version":"3.54.0"},"reference-count":53,"publisher":"The Company of Biologists","license":[{"start":{"date-parts":[[2018,1,1]],"date-time":"2018-01-01T00:00:00Z","timestamp":1514764800000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>Rainbow trout (Oncorhynchus mykiss) exposed to acid-base challenges activate branchial mechanisms for the excretion of acid-base equivalents. Current models of branchial acid-base excretion in freshwater rainbow trout propose two main ionocyte types; the peanut lectin agglutinin-positive (PNA+) mitochondrion-rich cell or ionocyte is believed to secrete HCO3\u2212 in exchange for Cl\u2212, whereas H+ secretion is thought to occur across PNA\u2212 ionocytes in exchange for Na+. Both HCO3\u2212 and H+ are supplied by intracellular hydration of CO2 catalyzed by cytosolic carbonic anhydrase (CAc). Immunohistochemical approaches revealed that under control conditions, CAc was detectable in 92.3\u00b11.0% (N=11) of PNA\u2212 ionocytes, and the abundance of PNA\u2212 ionocytes increased in response to systemic acidosis elicited by 72 h exposure to water of low pH (nominally pH 4.5), hypercapnia (1% CO2, nominally 7.6 Torr) or hyperoxia (achieved by gassing water with pure O2), as did the abundance of PNA\u2212 ionocytes that exhibited immunofluorescence for CAc. However, just 4.3 \u00b1 0.6% (N=11) of PNA+ ionocytes expressed detectable CAc under control conditions. Marked increases in the abundance of CAc-positive PNA+ ionocytes were detected following exposure of trout to a base load via recovery from hypercapnia, or base infusion (72 h infusion with 140 mmol L\u22121 NaHCO3). The percentage of CAc-positive PNA+ ionocytes also was increased in trout treated with cortisol (10 mg kg\u22121 hydrocortisone 21-hemisuccinate daily for 7 d). These results suggest that regulation of CA within PNA+ ionocytes and\/or the abundance of CAc-positive PNA+ ionocytes plays a role in activating base secretion in response to systemic alkalosis.<\/jats:p>","DOI":"10.1242\/jeb.164582","type":"journal-article","created":{"date-parts":[[2018,1,30]],"date-time":"2018-01-30T01:10:27Z","timestamp":1517274627000},"source":"Crossref","is-referenced-by-count":11,"title":["Carbonic anhydrase expression in the branchial ionocytes of rainbow trout"],"prefix":"10.1242","author":[{"given":"Michael","family":"Brannen","sequence":"first","affiliation":[{"name":"Department of Biology, University of Ottawa, Ottawa, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9056-4704","authenticated-orcid":false,"given":"Kathleen M.","family":"Gilmour","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Ottawa, Ottawa, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2018,1,1]]},"reference":[{"key":"2021042513214091200_JEB164582C1","doi-asserted-by":"publisher","first-page":"43","DOI":"10.1016\/j.cbpa.2014.11.001","article-title":"Mechanisms of Cl\u2212 uptake in rainbow trout: cloning and expression of slc26a6, a prospective Cl\u2212\/HCO3\u2212 exchanger","volume":"180","author":"Boyle","year":"2015","journal-title":"Comp. 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