{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T10:28:53Z","timestamp":1790245733200,"version":"4.1.0"},"reference-count":35,"publisher":"The Company of Biologists","license":[{"start":{"date-parts":[[2018,1,1]],"date-time":"2018-01-01T00:00:00Z","timestamp":1514764800000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>Detecting the presence of a parasite within its host is crucial to the study of host-parasite interactions. The Schistocephalus solidus - threespine stickleback pair has been studied extensively to investigate host phenotypic alterations associated with a parasite with a complex life cycle. This cestode is localized inside the stickleback's abdominal cavity and can be visually detected only once it passes a mass threshold. We present a non-lethal quantitative PCR approach based on detection of environmental DNA from the worm (eDNA), sampled in the fish abdominal cavity. Using this approach on two fish populations (n=151), 98% of fish were correctly assigned to their S. solidus infection status. There was a significant correlation between eDNA concentration and total parasitic mass. We also assessed ventilation rates as a complimentary mean to detect infection. Our eDNA detection method gives a reliable presence\/absence response and its future use for quantitative assessment is promising.<\/jats:p>","DOI":"10.1242\/jeb.178137","type":"journal-article","created":{"date-parts":[[2018,4,3]],"date-time":"2018-04-03T09:56:48Z","timestamp":1522749408000},"source":"Crossref","is-referenced-by-count":21,"title":["A eDNA-qPCR assay to detect the presence of the parasite\n                    <i>Schistocephalus solidus<\/i>\n                    inside its threespine stickleback host"],"prefix":"10.1242","author":[{"given":"Chlo\u00e9 Suzanne","family":"Berger","sequence":"first","affiliation":[{"name":"D\u00e9partement de Biologie et Institut de Biologie Int\u00e9grative et des Syst\u00e8mes (IBIS), Universit\u00e9 Laval, Qu\u00e9bec, G1V 0A6, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9030-634X","authenticated-orcid":false,"given":"Nadia","family":"Aubin-Horth","sequence":"additional","affiliation":[{"name":"D\u00e9partement de Biologie et Institut de Biologie Int\u00e9grative et des Syst\u00e8mes (IBIS), Universit\u00e9 Laval, Qu\u00e9bec, G1V 0A6, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2018,1,1]]},"reference":[{"key":"2021042422512593100_JEB178137C1","doi-asserted-by":"publisher","first-page":"4692","DOI":"10.1093\/nar\/25.22.4692","article-title":"Universal and rapid salt-extraction of high quality genomic DNA for PCR-based techniques","volume":"25","author":"Aljanabi","year":"1997","journal-title":"Nucleic Acids Res."},{"key":"2021042422512593100_JEB178137C2","doi-asserted-by":"publisher","first-page":"301","DOI":"10.1017\/S0031182000072103","article-title":"Infections of the three-spined stickleback, Gasterosteus aculeatus L., with the plerocercoid larvae of Schistocephalus solidus (M\u00fcller, 1776), with special reference to pathological effects","volume":"57","author":"Arme","year":"1967","journal-title":"Parasitology"},{"key":"2021042422512593100_JEB178137C3","doi-asserted-by":"publisher","first-page":"654","DOI":"10.1111\/j.1095-8649.1997.tb01521.x","article-title":"A non-invasive morphometric technique for estimating cestode plerocercoid burden in small freshwater fish","volume":"51","author":"Barber","year":"1997","journal-title":"J. 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