{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T17:21:10Z","timestamp":1773422470164,"version":"3.50.1"},"reference-count":38,"publisher":"The Company of Biologists","issue":"1","license":[{"start":{"date-parts":[[1994,10,1]],"date-time":"1994-10-01T00:00:00Z","timestamp":780969600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1994,10,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>In the presence of 6 mmol l\u22121 Ba2+, known to block the K+ channels in the basal membrane, a rise in bath [K+] ([K+]bl) induced an increase in intracellular K+ concentration ([K+]i) similar in amount and in time course to that obtained in the absence of Ba2+. The presence of active and passive (other than through K+ channels) K+ uptake mechanisms across the basal membrane was investigated in different bath K+ concentrations. Dihydro-ouabain (10\u22123 mol l\u22121), a blocker of the Na+\/K+-ATPase, tested in low bath [K+], and Sch28080 (10\u22124 mol l\u22121), a K+\/H+-ATPase inhibitor, were without effect on fluid secretion. Dihydro-ouabain was also without effect on electrical potential differences either in the absence or in the presence of Ba2+. Vanadate (10\u22123 mol l\u22121), in contrast, strongly reduced fluid secretion not only in control solution but also in high-K+, Na+-free medium and reduced the transepithelial and the apical membrane potential differences but not the basal membrane potential difference or [K+]i. Omitting Na+ from the bathing medium, replacing Cl-by Br\u2212 or applying bumetanide (10\u22125 mol l\u22121) inhibited fluid secretion only in a low-K+ (10 mmol l\u22121) medium. In 51 mmol l\u22121 [K+]bl, omitting Na+ was without effect and 10-4 mol l\u22121 bumetanide was needed to inhibit secretion. Replacing Cl\u2212by Br\u2212stimulated fluid secretion at this K+ concentration. Bumetanide (10\u22124 mol l\u22121) had no effect in 113 mmol l\u22121 [K+]bl. Bumetanide (10\u22124 mol l\u22121) in 51 mmol l\u22121 [K+]bl did not affect membrane potentials, did not lower [K+]i and did not affect the rise in [K+]i observed on an increase in [K+]bl. The results were summarized in a model proposing that K+ channels play a dominant role in high-K+ (113 mmol l\u22121) bathing medium. A K+\/Cl\u2212cotransporter may become more important in 51 mmol l\u22121 [K+]bl and a K+\/Na+\/2Cl\u2212 cotransporter may gain in importance in 10 mmol l\u22121 [K+]bl. Active mechanisms for K+ uptake across the basal membrane seem to play no detectable role in sustaining fluid secretion. The response to vanadate might be due to an effect on the apical electrogenic H+ pump.<\/jats:p>","DOI":"10.1242\/jeb.195.1.123","type":"journal-article","created":{"date-parts":[[2021,4,23]],"date-time":"2021-04-23T23:26:01Z","timestamp":1619220361000},"page":"123-145","source":"Crossref","is-referenced-by-count":50,"title":["Mechanisms of K+ Uptake Across the Basal Membrane of Malpighian Tubules of <i>Formica Polyctena<\/i>: The Effect of Ions and Inhibitors"],"prefix":"10.1242","volume":"195","author":[{"given":"A.","family":"Leyssens","sequence":"first","affiliation":[{"name":"Limburgs Universitair Centrum Laboratory of Physiology , , B3590 Diepenbeek, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Dijkstra","sequence":"additional","affiliation":[{"name":"Limburgs Universitair Centrum Laboratory of Physiology , , B3590 Diepenbeek, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Van Kerkhove","sequence":"additional","affiliation":[{"name":"Limburgs Universitair Centrum Laboratory of Physiology , , B3590 Diepenbeek, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Steels","sequence":"additional","affiliation":[{"name":"Limburgs Universitair Centrum Laboratory of Physiology , , B3590 Diepenbeek, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[1994,10,1]]},"reference":[{"key":"2023051512452085200_JEXBIO_195_1_123C1","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1016\/0300-9629(79)90001-X","article-title":"Ouabain-sensitivity of insect epithelial tissues","volume":"62A","author":"Anstee","year":"1979","journal-title":"Comp. 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