{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,8]],"date-time":"2026-02-08T00:57:35Z","timestamp":1770512255449,"version":"3.49.0"},"reference-count":59,"publisher":"The Company of Biologists","issue":"3","license":[{"start":{"date-parts":[[1995,3,1]],"date-time":"1995-03-01T00:00:00Z","timestamp":794016000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1995,3,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>The involvement of cyclic AMP in the settlement of the cypris larva of Balanus amphitrite amphitrite Darwin has been examined through the use of compounds that affect intracellular cyclic AMP levels. The activation of adenylate cyclase with forskolin, and the inhibition of phosphodiesterase with 3-isobutyl-1-methylxanthine, caffeine and theophylline, significantly increased the settlement of cyprids. Although the analogue dibutyryl cyclic AMP appeared to increase settlement, the effect was not significant. No marked increase in settlement resulted from the incubation of cyprids with dibutyryl cyclic GMP, 8-(4-chlorophenylthio) (CPT) cyclic AMP or papaverine (a phosphodiesterase inhibitor). Miconazole nitrate, an adenylate cyclase inhibitor, prevented settlement, but this effect appeared to be physico-chemical rather than pharmacological. Radioimmunoassay did not clearly show whether cyclic AMP levels changed following exposure of cyprids to a pulse of crude barnacle extract. However, exposure to forskolin significantly increased the cyclic AMP titre of cyprids. We conclude that compounds that alter intracellular cyclic AMP levels alter normal patterns of cyprid settlement. Whether this is because of an alteration in signal transduction is unclear.<\/jats:p>","DOI":"10.1242\/jeb.198.3.655","type":"journal-article","created":{"date-parts":[[2021,4,25]],"date-time":"2021-04-25T03:47:46Z","timestamp":1619322466000},"page":"655-664","source":"Crossref","is-referenced-by-count":86,"title":["Evidence for the Involvement of Cyclic Amp in the Pheromonal Modulation of Barnacle Settlement"],"prefix":"10.1242","volume":"198","author":[{"given":"Anthony S.","family":"Clare","sequence":"first","affiliation":[{"name":"Duke University 1 , , Marine Laboratory, 111 Pivers Island Road, Beaufort, NC 28516, USA"},{"name":"School of the Environment 1 , , Marine Laboratory, 111 Pivers Island Road, Beaufort, NC 28516, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ronald F.","family":"Thomas","sequence":"additional","affiliation":[{"name":"Duke University 1 , , Marine Laboratory, 111 Pivers Island Road, Beaufort, NC 28516, USA"},{"name":"School of the Environment 1 , , Marine Laboratory, 111 Pivers Island Road, Beaufort, NC 28516, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel","family":"Rittschof","sequence":"additional","affiliation":[{"name":"Duke University 1 , , Marine Laboratory, 111 Pivers Island Road, Beaufort, NC 28516, USA"},{"name":"School of the Environment 1 , , Marine Laboratory, 111 Pivers Island Road, Beaufort, NC 28516, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[1995,3,1]]},"reference":[{"key":"2024031623065511200_JEXBIO_198_3_655C1","first-page":"3","article-title":"Phylogeny of smell and taste","volume-title":"Smell and Taste in Health and Disease","author":"Ache","year":"1991"},{"key":"2024031623065511200_JEXBIO_198_3_655C2","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1093\/chemse\/16.5.421","article-title":"Odor recognition and olfactory transduction: the new frontier","volume":"16","author":"Anholt","year":"1991","journal-title":"Chem. Senses"},{"key":"2024031623065511200_JEXBIO_198_3_655C3","doi-asserted-by":"crossref","first-page":"1474","DOI":"10.1152\/jn.1990.64.5.1474","article-title":"Reduction of voltage-activated K+ currents by forskolin is not mediated via cAMP in pleural sensory neurons of Aplysia","volume":"64","author":"Baxter","year":"1990","journal-title":"J. Neurophysiol"},{"key":"2024031623065511200_JEXBIO_198_3_655C4","doi-asserted-by":"crossref","first-page":"1867","DOI":"10.1073\/pnas.84.7.1867","article-title":"G protein and diacylglycerol regulate metamorphosis of planktonic molluscan larvae","volume":"84","author":"Baxter","year":"1987","journal-title":"Proc. natn. Acad. Sci. U.S.A."},{"key":"2024031623065511200_JEXBIO_198_3_655C5","doi-asserted-by":"crossref","first-page":"147","DOI":"10.2307\/1542416","article-title":"Cilia from abalone larvae contain a receptor-dependent G protein transduction system similar to that in mammals","volume":"183","author":"Baxter","year":"1992","journal-title":"Biol. Bull. mar. biol. Lab., Woods Hole"},{"key":"2024031623065511200_JEXBIO_198_3_655C6","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1101\/SQB.1976.040.01.017","article-title":"Cyclic nucleotides, protein phosphorylation and synaptic function","volume":"40","author":"Beam","year":"1976","journal-title":"Cold Spring Harb. Symp. quant. Biol."},{"key":"2024031623065511200_JEXBIO_198_3_655C7","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/0022-0981(84)90215-6","article-title":"An investigation of low frequency sound waves as a means of inhibiting barnacle settlement","volume":"79","author":"Branscomb","year":"1984","journal-title":"J. exp. mar. Biol. Ecol."},{"key":"2024031623065511200_JEXBIO_198_3_655C8","doi-asserted-by":"crossref","first-page":"616","DOI":"10.1038\/256616a0","article-title":"Sticky actin","volume":"256","author":"Bray","year":"1975","journal-title":"Nature"},{"key":"2024031623065511200_JEXBIO_198_3_655C9","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1038\/345065a0","article-title":"Rapid kinetics of second messenger formation in olfactory transduction","volume":"345","author":"Breer","year":"1990","journal-title":"Nature"},{"key":"2024031623065511200_JEXBIO_198_3_655C10","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/0092-8674(91)90418-X","article-title":"A novel multigene family may encode odorant receptors: a molecular basis for odor recognition","volume":"65","author":"Buck","year":"1991","journal-title":"Cell"},{"key":"2024031623065511200_JEXBIO_198_3_655C11","doi-asserted-by":"crossref","first-page":"143","DOI":"10.2307\/1542415","article-title":"Recurring themes and variations: an overview and introduction","volume":"183","author":"Carr","year":"1992","journal-title":"Biol. Bull. mar. biol. Lab., Woods Hole"},{"key":"2024031623065511200_JEXBIO_198_3_655C12","first-page":"249","article-title":"Endocrinology of cirripedes","volume-title":"Barnacle Biology","author":"Clare","year":"1987"},{"key":"2024031623065511200_JEXBIO_198_3_655C13","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1017\/S0025315400035803","article-title":"On the antennular secretion of the cyprid of Balanus amphitrite amphitrite and its role as a settlement pheromone","volume":"74","author":"Clare","year":"1994","journal-title":"J. mar. biol. Ass. U.K."},{"key":"2024031623065511200_JEXBIO_198_3_655C14","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1002\/jez.1402620410","article-title":"Effects of the nonsteroidal ecdysone mimic RH 5849 on larval crustaceans","volume":"262","author":"Clare","year":"1992","journal-title":"J. exp. Zool."},{"key":"2024031623065511200_JEXBIO_198_3_655C15","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1080\/07924259.1992.9672258","article-title":"Molecular approaches to nontoxic antifouling","volume":"22","author":"Clare","year":"1992","journal-title":"Invert. Reprod. Dev."},{"key":"2024031623065511200_JEXBIO_198_3_655C16","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1007\/978-1-4615-9720-9_15","article-title":"Overview of research on marine invertebrate larvae, 1940\u20131980","volume-title":"Marine Biodeterioration: An Interdisciplinary Study","author":"Crisp","year":"1984"},{"key":"2024031623065511200_JEXBIO_198_3_655C17","first-page":"409","article-title":"Reduced discrimination of laboratory-reared cyprids of the barnacle Balanus amphitrite amphitrite Darwin, Crustacea Cirripedia, with a description of a common abnormality","volume-title":"Marine Biodeterioration","author":"Crisp","year":"1988"},{"key":"2024031623065511200_JEXBIO_198_3_655C18","first-page":"516","article-title":"Gregariousness and systematic affinity in some North Carolinian barnacles","volume":"47","author":"Crisp","year":"1990","journal-title":"Bull. mar. Sci."},{"key":"2024031623065511200_JEXBIO_198_3_655C19","doi-asserted-by":"crossref","first-page":"500","DOI":"10.1098\/rspb.1962.0052","article-title":"The chemical basis of gregariousness in cirripedes","volume":"156","author":"Crisp","year":"1962","journal-title":"Proc. R. Soc. Lond. B"},{"key":"2024031623065511200_JEXBIO_198_3_655C20","first-page":"362","article-title":"Adsorbed layers: the stimulus to settlement in barnacles","volume":"158","author":"Crisp","year":"1963","journal-title":"Proc. R. Soc. Lond. B"},{"key":"2024031623065511200_JEXBIO_198_3_655C21","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/0022-0981(92)90249-A","article-title":"Interactive effects of salinity and adult extract upon settlement of the estuarine barnacle Balanus improvisus (Darwin, 1854)","volume":"156","author":"Dineen","year":"1992","journal-title":"J. exp. mar. Biol. Ecol."},{"key":"2024031623065511200_JEXBIO_198_3_655C22","doi-asserted-by":"crossref","first-page":"1096","DOI":"10.1080\/01621459.1955.10501294","article-title":"A multiple comparison procedure for comparing several treatments with a control","volume":"50","author":"Dunnett","year":"1955","journal-title":"J. Am. statist. Assoc."},{"key":"2024031623065511200_JEXBIO_198_3_655C23","doi-asserted-by":"crossref","first-page":"482","DOI":"10.2307\/2528490","article-title":"New tables for multiple comparisons with a control","volume":"20","author":"Dunnett","year":"1964","journal-title":"Biometrics"},{"key":"2024031623065511200_JEXBIO_198_3_655C24","doi-asserted-by":"crossref","first-page":"907","DOI":"10.1016\/0896-6273(92)90243-7","article-title":"Plasma membrane inositol 1,4,5,-trisphosphate-activated channels mediate signal transduction in lobster olfactory receptor neurons","volume":"9","author":"Fadool","year":"1992","journal-title":"Neuron"},{"key":"2024031623065511200_JEXBIO_198_3_655C25","first-page":"518","article-title":"G-proteins and inositol-phospholipid metabolism implicated in odor response of cultured lobster olfactory neurons","volume":"16","author":"Fadool","year":"1991","journal-title":"Chem. Senses"},{"key":"2024031623065511200_JEXBIO_198_3_655C26","first-page":"377","article-title":"The chemical basis of gregariousness in cirripedes: a review","volume-title":"Barnacle Biology","author":"Gabbott","year":"1987"},{"key":"2024031623065511200_JEXBIO_198_3_655C27","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1113\/jphysiol.1978.sp012189","article-title":"Characterization and ionic basis of GABA-induced depolarizations recorded in vitro from cat afferent primary neurones","volume":"275","author":"Gallagher","year":"1978","journal-title":"J. Physiol., Lond."},{"key":"2024031623065511200_JEXBIO_198_3_655C28","doi-asserted-by":"crossref","first-page":"1903","DOI":"10.1007\/BF01013485","article-title":"Chemical ecology and the search for marine antifoulants: studies of a predator-prey symbiosis","volume":"14","author":"Gerhart","year":"1988","journal-title":"J. chem. Ecol."},{"key":"2024031623065511200_JEXBIO_198_3_655C29","first-page":"227","article-title":"Concerning the fourth antennular segment of Balanus balanoides","volume-title":"Fourth European Marine Biology Symposium","author":"Gibson","year":"1971"},{"key":"2024031623065511200_JEXBIO_198_3_655C30","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1007\/BF01016500","article-title":"Chemically induced metamorphosis of polychaete larvae in both the laboratory and ocean environment","volume":"16","author":"Jensen","year":"1990","journal-title":"J. chem. Ecol."},{"key":"2024031623065511200_JEXBIO_198_3_655C31","doi-asserted-by":"crossref","first-page":"584","DOI":"10.1242\/jeb.30.4.584","article-title":"Laboratory experiments on gregariousness during settling in Balanus balanoides and other barnacles","volume":"30","author":"Knight-Jones","year":"1953","journal-title":"J. exp. Biol."},{"key":"2024031623065511200_JEXBIO_198_3_655C32","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1038\/175266a0","article-title":"The gregarious setting reaction of barnacles as a measure of their systematic affinity","volume":"174","author":"Knight-Jones","year":"1955","journal-title":"Nature"},{"key":"2024031623065511200_JEXBIO_198_3_655C33","doi-asserted-by":"crossref","first-page":"1109","DOI":"10.1038\/1711109a0","article-title":"Gregariousness in barnacles in relation to the fouling of ships and to antifouling research","volume":"171","author":"Knight-Jones","year":"1953","journal-title":"Nature"},{"key":"2024031623065511200_JEXBIO_198_3_655C34","first-page":"73","article-title":"Protein extracts from some marine animals which promote barnacle settlement: possible relationship between a protein component of arthropod cuticle and actin","volume":"77B","author":"Larman","year":"1984","journal-title":"Comp. Biochem. Physiol."},{"key":"2024031623065511200_JEXBIO_198_3_655C35","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1016\/S0165-6147(89)80008-2","article-title":"Forskolin: a specific stimulator of adenylyl cyclase or a diterpene with multiple sites of action?","volume":"10","author":"Laurenza","year":"1989","journal-title":"Trends pharmac. Sci."},{"key":"2024031623065511200_JEXBIO_198_3_655C36","doi-asserted-by":"crossref","first-page":"3979","DOI":"10.1523\/JNEUROSCI.12-10-03979.1992","article-title":"Cyclic nucleotides mediate an odor-evoked potassium conductance in lobster olfactory receptor cells","volume":"12","author":"Michel","year":"1992","journal-title":"J. Neurosci."},{"key":"2024031623065511200_JEXBIO_198_3_655C37","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1016\/S0021-9258(19)41916-9","article-title":"Induction of hepatic tyrosine aminotransferase in vivo by derivatives of cyclic adenosine 3,5 -monophosphate","volume":"250","author":"Miller","year":"1975","journal-title":"J. biol. Chem"},{"key":"2024031623065511200_JEXBIO_198_3_655C38","first-page":"465","article-title":"Recent progress in larval settlement and metamorphosis: closing the gaps between molecular biology and ecology","volume":"46","author":"Morse","year":"1990","journal-title":"Bull. mar. Sci."},{"key":"2024031623065511200_JEXBIO_198_3_655C39","first-page":"107","article-title":"Molecular mechanisms controlling metamorphosis and recruitment in abalone larvae","volume-title":"Abalones of the World","author":"Morse","year":"1992"},{"key":"2024031623065511200_JEXBIO_198_3_655C40","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1038\/309158a0","article-title":"Role for microsomal Ca++ storage in mammalian neurones","volume":"309","author":"Neering","year":"1984","journal-title":"Nature"},{"key":"2024031623065511200_JEXBIO_198_3_655C41","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1007\/BF00351147","article-title":"Settlement of barnacle larvae: surface structure of the antennular attachment disc by scanning electron microscopy","volume":"2","author":"Nott","year":"1969","journal-title":"Mar. Biol."},{"key":"2024031623065511200_JEXBIO_198_3_655C42","first-page":"115","article-title":"On the structure of the antennular attachment organ of the cypris larva of Balanus balanoides (L","volume":"256","author":"Nott","year":"1969","journal-title":"Phil. Trans. R. Soc. B"},{"key":"2024031623065511200_JEXBIO_198_3_655C43","first-page":"512","article-title":"Natural and artificial induction of metamorphosis of Phragmatopoma lapidosa californica (Polychaeta: Sabellariidae), with a critical look at the effects of bioactive compounds on marine invertebrate larvae","volume":"46","author":"Pawlik","year":"1990","journal-title":"Bull. mar. Sci."},{"key":"2024031623065511200_JEXBIO_198_3_655C44","first-page":"189","article-title":"Induction of marine invertebrate larval settlement: evidence for chemical cues","volume-title":"Ecological Roles of Marine Natural Products","author":"Pawlik","year":"1992"},{"key":"2024031623065511200_JEXBIO_198_3_655C45","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1007\/BF00346346","article-title":"Influence of delayed metamorphosis on survival and growth of juvenile barnacles Balanus amphitrite","volume":"115","author":"Pechenik","year":"1993","journal-title":"Mar. Biol."},{"key":"2024031623065511200_JEXBIO_198_3_655C46","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1093\/icb\/33.6.487-a","article-title":"Body odors and neutral-basic peptide mimics: a review of responses by marine organisms","volume":"33","author":"Rittschof","year":"1993","journal-title":"Am. Zool."},{"key":"2024031623065511200_JEXBIO_198_3_655C47","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/0022-0981(84)90099-6","article-title":"Settlement and behaviour in relation to flow and surface in larval barnacles, Balanus amphitrite Darwin","volume":"82","author":"Rittschof","year":"1984","journal-title":"J. exp. mar. Biol. Ecol."},{"key":"2024031623065511200_JEXBIO_198_3_655C48","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1080\/08927019209386217","article-title":"Barnacle in vitro assays for biologically active substances: toxicity and settlement inhibition assays using mass cultured Balanus amphitrite amphitrite Darwin","volume":"6","author":"Rittschof","year":"1992","journal-title":"Biofouling"},{"key":"2024031623065511200_JEXBIO_198_3_655C49","first-page":"411","article-title":"Surface determination of macroinvertebrate larval settlement","volume-title":"Proceedings of the 21st European Marine Biology Symposium","author":"Rittschof","year":"1989"},{"key":"2024031623065511200_JEXBIO_198_3_655C50","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1016\/0077-7579(86)90048-7","article-title":"Ion and neuropharmacological studies of barnacle settlement","volume":"20","author":"Rittschof","year":"1986","journal-title":"Neth. J. Sea Res."},{"key":"2024031623065511200_JEXBIO_198_3_655C51","first-page":"317","article-title":"Molecular mediation of settlement of selected invertebrate larvae","volume-title":"Bioactive Compounds from Marine Organisms","author":"Rittschof","year":"1991"},{"key":"2024031623065511200_JEXBIO_198_3_655C52","doi-asserted-by":"crossref","first-page":"3363","DOI":"10.1073\/pnas.78.6.3363","article-title":"Forskolin: unique diterpene activator of adenylate cyclase in membranes and intact cells","volume":"78","author":"Seamon","year":"1981","journal-title":"Proc. natn. Acad. Sci. U.S.A"},{"key":"2024031623065511200_JEXBIO_198_3_655C53","doi-asserted-by":"crossref","first-page":"699","DOI":"10.1210\/endo-125-2-699","article-title":"Nitroimidazole derivatives inhibit anterior pituitary cell function apparently by a direct effect on the catalytic subunit of the adenylate cyclase holoenzyme","volume":"125","author":"Stalla","year":"1989","journal-title":"Endocrinology"},{"key":"2024031623065511200_JEXBIO_198_3_655C54","doi-asserted-by":"crossref","first-page":"1106","DOI":"10.1016\/S0021-9258(19)45621-4","article-title":"Radioimmunoassay for cyclic nucleotides. I. Preparation of antibodies and iodinated cyclic nucleotides","volume":"247","author":"Steiner","year":"1972","journal-title":"J. biol. Chem."},{"key":"2024031623065511200_JEXBIO_198_3_655C55","doi-asserted-by":"crossref","first-page":"1403","DOI":"10.1016\/0196-9781(88)90209-4","article-title":"Synthetic peptide analogs to barnacle settlement pheromone","volume":"9","author":"Tegtmeyer","year":"1989","journal-title":"Peptides"},{"key":"2024031623065511200_JEXBIO_198_3_655C56","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1080\/08927019009378135","article-title":"Settlement of Chthamalus montagui Southward cyprids on barnacle arthropodin","volume":"2","author":"Whillis","year":"1990","journal-title":"Biofouling"},{"key":"2024031623065511200_JEXBIO_198_3_655C57","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1242\/jeb.161.1.423","article-title":"The role of cyclic AMP in the octopaminergic modulation of flight muscle in the locust","volume":"161","author":"Whim","year":"1991","journal-title":"J. exp. Biol."},{"key":"2024031623065511200_JEXBIO_198_3_655C58","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1017\/S002531540007065X","article-title":"Adhesion of cypris larvae of the barnacle, Balanus balanoides, to clean and arthropodin treated surfaces","volume":"63","author":"Yule","year":"1983","journal-title":"J. mar. biol. Ass. U.K."},{"key":"2024031623065511200_JEXBIO_198_3_655C59","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1017\/S0025315400030101","article-title":"The temporary adhesion of barnacle cyprids: effects of some differing surface characteristics","volume":"64","author":"Yule","year":"1984","journal-title":"J. mar. biol. Ass. U.K."}],"container-title":["Journal of Experimental Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.biologists.com\/jeb\/article-pdf\/198\/3\/655\/3385422\/jexbio_198_3_655.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.biologists.com\/jeb\/article-pdf\/198\/3\/655\/3385422\/jexbio_198_3_655.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,3,16]],"date-time":"2024-03-16T23:07:21Z","timestamp":1710630441000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.biologists.com\/jeb\/article\/198\/3\/655\/6893\/Evidence-for-the-Involvement-of-Cyclic-Amp-in-the"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,3,1]]},"references-count":59,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1995,3,1]]}},"URL":"https:\/\/doi.org\/10.1242\/jeb.198.3.655","relation":{},"ISSN":["0022-0949","1477-9145"],"issn-type":[{"value":"0022-0949","type":"print"},{"value":"1477-9145","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,3,1]]}}}