{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,21]],"date-time":"2025-09-21T18:13:59Z","timestamp":1758478439157},"reference-count":18,"publisher":"The Company of Biologists","issue":"8","license":[{"start":{"date-parts":[[1995,8,1]],"date-time":"1995-08-01T00:00:00Z","timestamp":807235200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1995,8,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Kinesin is a motor protein that converts chemical energy derived from ATP hydrolysis into mechanical work to transport cellular components along microtubules. We studied the properties of ATP-dependent microtubule\u2013 kinesin sliding with two different in vitro assay systems. In one assay system, a kinesin-coated glass microneedle (elastic coefficient, 1\u20132.5 pN \u03bcm\u22121) was made to slide along an axoneme. Using this system, we obtained the relationship between the force (=load) on the microneedle and the velocity of microneedle\u2013kinesin sliding in the auxotonic condition, in which the load on the microtubule\u2013kinesin contacts increased as sliding progressed. The force\u2013velocity curve was upwardly convex (maximum velocity Vmax, 0.58\u00b10.15 \u03bcm s\u22121; maximum isometric force P0, 5.0\u00b11.6 pN) and was similar to that of in vitro actin\u2013myosin sliding in the auxotonic condition, suggesting that the two motor protein systems have fundamental kinetic properties in common. In the other assay system, an axoneme attached to a glass microneedle (elastic coefficient, 4\u20135 pN \u03bcm\u22121) was made to slide on a kinesin-coated glass surface (Vmax, 0.68\u00b10.17 \u03bcm s\u22121; P0, 46.1\u00b118.6 pN). The change in shape of the axoneme indicated an enormous flexibility of randomly oriented kinesin molecules.<\/jats:p>","DOI":"10.1242\/jeb.198.8.1809","type":"journal-article","created":{"date-parts":[[2021,4,25]],"date-time":"2021-04-25T03:53:15Z","timestamp":1619322795000},"page":"1809-1815","source":"Crossref","is-referenced-by-count":2,"title":["The Mode Of ATP-Dependent Microtubule\u2013Kinesin Sliding In The Auxotonic Condition"],"prefix":"10.1242","volume":"198","author":[{"given":"I.","family":"Shirakawa","sequence":"first","affiliation":[{"name":"Teikyo University 1 Department of Physiology, School of Medicine , , Itabashi-ku, Tokyo 173, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Oiwa","sequence":"additional","affiliation":[{"name":"Teikyo University 1 Department of Physiology, School of Medicine , , Itabashi-ku, Tokyo 173, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Chaen","sequence":"additional","affiliation":[{"name":"Teikyo University 1 Department of Physiology, School of Medicine , , Itabashi-ku, Tokyo 173, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Shimizu","sequence":"additional","affiliation":[{"name":"2 National Institute of Bioscience and Human Technology, Tsukuba, Ibaraki 305, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Tanaka","sequence":"additional","affiliation":[{"name":"Teikyo University 1 Department of Physiology, School of Medicine , , Itabashi-ku, Tokyo 173, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Sugi","sequence":"additional","affiliation":[{"name":"Teikyo University 1 Department of Physiology, School of Medicine , , Itabashi-ku, Tokyo 173, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[1995,8,1]]},"reference":[{"key":"2024031720464767700_JEXBIO_198_8_1809C1","first-page":"373","article-title":"Preparation and purification of dynein","volume-title":"Methods in Cell Biology","author":"Bell","year":"1982"},{"key":"2024031720464767700_JEXBIO_198_8_1809C2","doi-asserted-by":"publisher","first-page":"348","DOI":"10.1038\/348348a0","article-title":"Bead movement by single kinesin molecules studied with optical tweezers","volume":"348","author":"Block","year":"1990","journal-title":"Nature"},{"key":"2024031720464767700_JEXBIO_198_8_1809C3","doi-asserted-by":"publisher","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein\u2013dye binding","volume":"72","author":"Bradford","year":"1976","journal-title":"Analyt. 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