{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T11:12:57Z","timestamp":1783336377175,"version":"3.54.6"},"reference-count":67,"publisher":"The Company of Biologists","issue":"8","license":[{"start":{"date-parts":[[1996,8,1]],"date-time":"1996-08-01T00:00:00Z","timestamp":838857600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1996,8,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Australian desert frogs of the genera Neobatrachus, Cyclorana and Heleioporus experience significant dehydration, and iono- and osmoconcentration, during aestivation in the laboratory and accumulate substantial amounts of urea (100\u2013200 mmol l\u22121). We expected a priori that aestivating frogs probably would not need to accumulate balancing osmolytes but would accumulate trimethylamine oxide (TMAO) or betaine as counteracting solutes to urea. These aestivating frogs did not co-accumulate a substantial quantity of any particular balancing osmolyte or counteracting solute, such as a methylamine [TMAO, trimethylamine amine (TMA), betaine, sarcosine, glycerophosphorylcholine (GPC)] or polyol (inositol, mannitol, sorbitol) in plasma or muscle relative to urea accumulation. However, for aestivating frogs, the total concentration of all measured methylamines and polyols (TMAO + TMA + betaine + sarcosine + GPC + inositol) in muscle was approximately 35\u201345 mmol kg\u22121, and so it is possible that all of these solutes have a combined counteracting osmolyte role in aestivating frogs at a ratio to urea of approximately 1:2.5, as has been described for elasmobranch fishes. Alternatively, the absence of substantial co-accumulation with urea of any particular solute suggests that aestivating frogs might not require any major extracellular or intracellular counteracting solutes (TMAO, betaine, GPC). The enzyme systems of these aestivating frogs may be insensitive to the perturbing effects of urea, or the perturbing effects of accumulated urea may be a mechanism for metabolic depression, during aestivation.<\/jats:p>","DOI":"10.1242\/jeb.199.8.1809","type":"journal-article","created":{"date-parts":[[2021,4,25]],"date-time":"2021-04-25T04:12:45Z","timestamp":1619323965000},"page":"1809-1816","source":"Crossref","is-referenced-by-count":78,"title":["Do Australian Desert Frogs Co-Accumulate Counteracting Solutes with Urea During Aestivation?"],"prefix":"10.1242","volume":"199","author":[{"given":"Philip C.","family":"Withers","sequence":"first","affiliation":[{"name":"University of Western Australia 1 Department of Zoology , , Nedlands, WA 6907, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Guppy","sequence":"additional","affiliation":[{"name":"University of Western Australia 2 Department of Biochemistry , , Nedlands, WA 6907, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[1996,8,1]]},"reference":[{"key":"2024020322293065700_JEXBIO_199_8_1809C1","first-page":"313","article-title":"Adaptations to salinity and osmoregulation in the frog Thoropa miliaris (Amphibia, Leptodactylidae)","volume":"227","author":"Abe","year":"1991","journal-title":"Zool. 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Physiol."},{"key":"2024020322293065700_JEXBIO_199_8_1809C38","doi-asserted-by":"crossref","first-page":"209","DOI":"10.2307\/1442478","article-title":"Changes in body fluids of burrowed spadefoot toads as a function of soil water potential","volume":"1972","author":"Mcclanahan","year":"1972","journal-title":"Copeia"},{"key":"2024020322293065700_JEXBIO_199_8_1809C39","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1002\/jez.1402710302","article-title":"The mechanism of glycine betaine regulation in response to hyperosmotic stress in oyster mitochondria: A comparative study of Atlantic and Chesapeake Bay oysters","volume":"271","author":"Pierce","year":"1995","journal-title":"J. exp. Zool."},{"key":"2024020322293065700_JEXBIO_199_8_1809C40","doi-asserted-by":"crossref","first-page":"1059","DOI":"10.1016\/S0021-9258(18)64242-5","article-title":"Competitive inhibition of enzyme activity by urea","volume":"236","author":"Rajagopalan","year":"1961","journal-title":"J. biol. 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Physiol."},{"key":"2024020322293065700_JEXBIO_199_8_1809C44","doi-asserted-by":"crossref","first-page":"400","DOI":"10.2307\/1444229","article-title":"Osmoregulation of the terrestrial salamander, Ambystoma tigrinum, in hypersaline media","volume":"1981","author":"Romspert","year":"1981","journal-title":"Copeia"},{"key":"2024020322293065700_JEXBIO_199_8_1809C45","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1242\/jeb.138.1.333","article-title":"Ion replacement as a buoyancy mechanism in a pelagic deep-sea crustacean","volume":"138","author":"Sanders","year":"1988","journal-title":"J. exp. Biol."},{"key":"2024020322293065700_JEXBIO_199_8_1809C46","first-page":"275","article-title":"Osmotic and ionic regulation in Xenopus laevis Daud. during adaptation to different osmotic environment","volume":"77","author":"Schlisio","year":"1973","journal-title":"Zool. Jb. Physiol."},{"key":"2024020322293065700_JEXBIO_199_8_1809C47","first-page":"23","article-title":"L\u2019osmoregulatione chez les batrachiens","volume":"96","author":"Schoffeniels","year":"1965","journal-title":"Annls Soc. r. zool. Belg."},{"key":"2024020322293065700_JEXBIO_199_8_1809C48","first-page":"129","article-title":"Preliminary studies on the effects of salinity on intracellular nitrogenous osmolytes in various tissues and hemolymph of the Japanese spiny lobster, Panuliris japonicus (Von Siebold, 1824)","volume":"68","author":"Shinagawa","year":"1995","journal-title":"Crustaceana"},{"key":"2024020322293065700_JEXBIO_199_8_1809C49","first-page":"125","article-title":"Exchange of water, ions and respiratory gases in terrestrial amphibians","volume-title":"Environmental Physiology of the Amphibia","author":"Shoemaker","year":"1992"},{"key":"2024020322293065700_JEXBIO_199_8_1809C50","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1515\/znc-1995-1-217","article-title":"Polyols accumulate in renal tissue uncouple the plasma membrane calcium pump and counteract inhibition by urea and guanidine hydrochloride","volume":"50","author":"Sola-Penna","year":"1995","journal-title":"Z. Naturforsch. 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