{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T05:31:30Z","timestamp":1792647090518,"version":"4.5.0"},"reference-count":52,"publisher":"The Company of Biologists","license":[{"start":{"date-parts":[[2021,5,18]],"date-time":"2021-05-18T00:00:00Z","timestamp":1621296000000},"content-version":"am","delay-in-days":868,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R35 NS 097343"],"award-info":[{"award-number":["R35 NS 097343"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>The heart and pyloric rhythms of crustaceans have been studied separately and extensively over many years. Local and hormonal neuromodulation and sensory inputs onto these central pattern generator circuits play a significant role in an animals\u2019 response to perturbations, but are usually lost or removed during in vitro studies. To examine simultaneously the in vivo motor output of the crustacean heart and pyloric rhythms, we used photoplethysmography (PPG). In the population measured (n=49), the heart rhythm frequencies ranged from 0.3-2.3 Hz. The pyloric rhythms varied from 0.2-1.6 Hz. We observed a weak correlation between the frequencies of the heart and pyloric rhythms. During multiple hour-long recordings, many animals held at a controlled temperature showed strong inhibitory bouts in which the heart decreased in frequency or become quiescent and the pyloric rhythm decreased in frequency. We measured the simultaneous responses of the rhythms to temperature ramps by heating or cooling the saline bath while recording both the heart and pyloric muscle movements. Q10s, critical temperatures (temperatures at which muscle function is compromised), and changes in frequency were calculated for each of the rhythms tested. The heart rhythm was more robust to high temperature than the pyloric rhythm.<\/jats:p>","DOI":"10.1242\/jeb.199190","type":"journal-article","created":{"date-parts":[[2019,1,10]],"date-time":"2019-01-10T12:26:22Z","timestamp":1547123182000},"source":"Crossref","is-referenced-by-count":35,"title":["<i>In vivo<\/i>\n                    effects of temperature on the heart and pyloric rhythms in the crab,\n                    <i>Cancer borealis<\/i>"],"prefix":"10.1242","author":[{"given":"Dahlia","family":"Kushinsky","sequence":"first","affiliation":[{"name":"Biology Department and Volen Center, Brandeis University, Waltham, MA 02454, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ekaterina O.","family":"Morozova","sequence":"additional","affiliation":[{"name":"Biology Department and Volen Center, Brandeis University, Waltham, MA 02454, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9632-5448","authenticated-orcid":false,"given":"Eve","family":"Marder","sequence":"additional","affiliation":[{"name":"Biology Department and Volen Center, Brandeis University, Waltham, MA 02454, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2019,1,1]]},"reference":[{"key":"2021042513312142600_JEB199190C1","doi-asserted-by":"publisher","first-page":"175","DOI":"10.1017\/S0025315400011498","article-title":"Some experiments on the function of the pericardial organs in Crustacea","volume":"32","author":"Alexandrowicz","year":"1953","journal-title":"J. Mar. Biol. Assoc. UK"},{"key":"2021042513312142600_JEB199190C2","first-page":"7","volume-title":"A Gentle Tutorial of the EM Algorithm and its Application to Parameter Estimation for Gaussian Mixture and Hidden Markov Models","author":"Bilmes","year":"1998"},{"key":"2021042513312142600_JEB199190C3","article-title":"Maximum entropy spectral analysis","volume-title":"Proceedings of the 37th Meeting Society of Exploration Geophysicist","author":"Burg","year":"1967"},{"key":"2021042513312142600_JEB199190C4","volume-title":"Spectral Analysis and Filter Theory","author":"Buttkus","year":"2000"},{"key":"2021042513312142600_JEB199190C5","doi-asserted-by":"publisher","first-page":"1327","DOI":"10.1007\/s00359-006-0162-1","article-title":"Temperature acclimation alters cardiac performance in the lobster Homarus americanus","volume":"192","author":"Camacho","year":"2006","journal-title":"J. Comp. Physiol. A Neuroethol. Sens. Neural Behav. Physiol."},{"key":"2021042513312142600_JEB199190C6","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1093\/czoolo\/56.5.499","article-title":"Circatidal rhythms of locomotion in the American horseshoe crab Limulus polyphemus: underlying mechanisms and cues that influence them","volume":"56","author":"Chabot","year":"2010","journal-title":"Curr. Zool."},{"key":"2021042513312142600_JEB199190C7","doi-asserted-by":"crossref","DOI":"10.1093\/czoolo\/56.5.i","article-title":"Horseshoe crab behavior: patterns and processes","volume":"56","author":"Chabot","year":"2010","journal-title":"Curr. Zool."},{"key":"2021042513312142600_JEB199190C8","doi-asserted-by":"publisher","first-page":"818","DOI":"10.1021\/pr900736t","article-title":"Mass spectral analysis of neuropeptide expression and distribution in the nervous system of the lobster Homarus americanus","volume":"9","author":"Chen","year":"2010","journal-title":"J. Proteome Res."},{"key":"2021042513312142600_JEB199190C9","doi-asserted-by":"crossref","first-page":"2431","DOI":"10.1242\/jeb.198.12.2431","article-title":"Matrix of neuromodulators in neurosecretory structures of the crab Cancer borealis","volume":"198","author":"Christie","year":"1995","journal-title":"J. Exp. Biol."},{"key":"2021042513312142600_JEB199190C10","doi-asserted-by":"publisher","first-page":"1909","DOI":"10.1016\/j.peptides.2008.07.010","article-title":"Identification and cardiotropic actions of brain\/gut-derived tachykinin-related peptides (TRPs) from the American lobster Homarus americanus","volume":"29","author":"Christie","year":"2008","journal-title":"Peptides"},{"key":"2021042513312142600_JEB199190C11","doi-asserted-by":"publisher","first-page":"4135","DOI":"10.1007\/s00018-010-0482-8","article-title":"Crustacean neuropeptides","volume":"67","author":"Christie","year":"2010","journal-title":"Cell. Mol. Life Sci."},{"key":"2021042513312142600_JEB199190C12","doi-asserted-by":"publisher","first-page":"1396","DOI":"10.1152\/jn.1998.79.3.1396","article-title":"Long-term expression of two interacting motor pattern-generating networks in the stomatogastric system of freely behaving lobster","volume":"79","author":"Clemens","year":"1998","journal-title":"J. Neurophysiol."},{"key":"2021042513312142600_JEB199190C13","doi-asserted-by":"publisher","first-page":"2788","DOI":"10.1523\/JNEUROSCI.18-07-02788.1998","article-title":"In vivo modulation of interacting central pattern generators in lobster stomatogastric ganglion: influence of feeding and partial pressure of oxygen","volume":"18","author":"Clemens","year":"1998","journal-title":"J. Neurosci."},{"key":"2021042513312142600_JEB199190C14","doi-asserted-by":"publisher","first-page":"65","DOI":"10.1016\/S0304-3940(98)00511-4","article-title":"Feeding-induced changes in temporal patterning of muscle activity in the lobster stomatogastric system","volume":"254","author":"Clemens","year":"1998","journal-title":"Neurosci. Lett."},{"key":"2021042513312142600_JEB199190C15","doi-asserted-by":"crossref","first-page":"817","DOI":"10.1242\/jeb.202.7.817","article-title":"Changes in motor network expression related to moulting behaviour in lobster: role of moult-induced deep hypoxia","volume":"202","author":"Clemens","year":"1999","journal-title":"J. Exp. Biol."},{"key":"2021042513312142600_JEB199190C16","doi-asserted-by":"publisher","first-page":"299","DOI":"10.1016\/S0034-5687(01)00308-5","article-title":"A modulatory role for oxygen in shaping rhythmic motor output patterns of neuronal networks","volume":"128","author":"Clemens","year":"2001","journal-title":"Respir. Physiol."},{"key":"2021042513312142600_JEB199190C17","doi-asserted-by":"publisher","first-page":"108","DOI":"10.2307\/1543649","article-title":"Reliable, responsive pacemaking and pattern generation with minimal cell numbers: the crustacean cardiac ganglion","volume":"202","author":"Cooke","year":"2002","journal-title":"Biol. Bull."},{"key":"2021042513312142600_JEB199190C18","doi-asserted-by":"publisher","first-page":"2873","DOI":"10.1242\/jeb.002949","article-title":"Multiple modulators act on the cardiac ganglion of the crab, Cancer borealis","volume":"210","author":"Cruz-Bermudez","year":"2007","journal-title":"J. Exp. Biol."},{"key":"2021042513312142600_JEB199190C19","first-page":"1394","article-title":"Biological clocks and rhythms in intertidal crustaceans","volume":"2","author":"De La Iglesia","year":"2010","journal-title":"Frontier. Biosci."},{"key":"2021042513312142600_JEB199190C20","doi-asserted-by":"publisher","first-page":"1782","DOI":"10.1021\/pr060603v","article-title":"Imaging mass spectrometry of neuropeptides in decapod crustacean neuronal tissues","volume":"6","author":"DeKeyser","year":"2007","journal-title":"J. Proteome Res."},{"key":"2021042513312142600_JEB199190C21","first-page":"369","article-title":"Photoplethysmography \u2013 a non-invasive technique for monitoring heart beat and ventilation rate in Decapod Crustaceans","volume":"77A","author":"Depledge","year":"1983","journal-title":"Comp. Biochem. Physiol."},{"key":"2021042513312142600_JEB199190C22","doi-asserted-by":"publisher","first-page":"149","DOI":"10.1016\/j.conb.2016.09.010","article-title":"Neuropeptide modulation of pattern-generating systems in crustaceans: comparative studies and approaches","volume":"41","author":"Dickinson","year":"2016","journal-title":"Curr. Opin. Neurobiol."},{"key":"2021042513312142600_JEB199190C23","doi-asserted-by":"publisher","first-page":"609","DOI":"10.1016\/j.neuron.2018.08.035","article-title":"Circuit robustness to temperature perturbation is altered by neuromodulators","volume":"100","author":"Haddad","year":"2018","journal-title":"Neuron"},{"key":"2021042513312142600_JEB199190C24","doi-asserted-by":"publisher","first-page":"e41877","DOI":"10.7554\/eLife.41877","article-title":"Two central pattern generators from the crab Cancer borealis respond robustly and differentially to extreme extracellular pH","volume":"7","author":"Haley","year":"2018","journal-title":"eLife"},{"key":"2021042513312142600_JEB199190C25","doi-asserted-by":"publisher","first-page":"21","DOI":"10.1101\/sqb.2014.79.024828","article-title":"Animal-to-animal variability in neuromodulation and circuit function","volume":"79","author":"Hamood","year":"2015","journal-title":"Cold Spring Harb. Symp. Quant. Biol."},{"key":"2021042513312142600_JEB199190C26","doi-asserted-by":"publisher","first-page":"528","DOI":"10.1152\/jn.1988.59.2.528","article-title":"Gastric mill activity in the lobster. I. Spontaneous modes of chewing","volume":"59","author":"Heinzel","year":"1988","journal-title":"J. Neurophysiol."},{"key":"2021042513312142600_JEB199190C27","doi-asserted-by":"publisher","first-page":"566","DOI":"10.1152\/jn.1988.59.2.566","article-title":"Gastric mill activity in the lobster. III. Effects of proctolin on the isolated central pattern generator","volume":"59","author":"Heinzel","year":"1988","journal-title":"J. Neurophysiol."},{"key":"2021042513312142600_JEB199190C28","doi-asserted-by":"publisher","first-page":"2097","DOI":"10.1523\/JNEUROSCI.07-07-02097.1987","article-title":"Modulation of the lobster pyloric rhythm by the peptide proctolin","volume":"7","author":"Hooper","year":"1987","journal-title":"J. Neurosci."},{"key":"2021042513312142600_JEB199190C29","doi-asserted-by":"publisher","first-page":"230","DOI":"10.1016\/j.peptides.2012.05.007","article-title":"Mass spectrometric elucidation of the neuropeptidome of a crustacean neuroendocrine organ","volume":"36","author":"Hui","year":"2012","journal-title":"Peptides"},{"key":"2021042513312142600_JEB199190C30","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.neuron.2012.09.010","article-title":"Neuromodulation of neuronal circuits: back to the future","volume":"76","author":"Marder","year":"2012","journal-title":"Neuron"},{"key":"2021042513312142600_JEB199190C31","doi-asserted-by":"publisher","first-page":"291","DOI":"10.1146\/annurev.physiol.69.031905.161516","article-title":"Understanding circuit dynamics using the stomatogastric nervous system of lobsters and crabs","volume":"69","author":"Marder","year":"2007","journal-title":"Annu. Rev. Physiol."},{"key":"2021042513312142600_JEB199190C32","doi-asserted-by":"publisher","first-page":"851","DOI":"10.1007\/s00359-014-0975-2","article-title":"How can motor systems retain performance over a wide temperature range? Lessons from the crustacean stomatogastric nervous system","volume":"201","author":"Marder","year":"2015","journal-title":"J. Comp. Physiol. A Neuroethol. Sens. Neural Behav. Physiol."},{"key":"2021042513312142600_JEB199190C33","doi-asserted-by":"publisher","first-page":"59","DOI":"10.1111\/j.1749-6632.1972.tb27823.x","article-title":"Simpler networks","volume":"193","author":"Maynard","year":"1972","journal-title":"Ann. NY Acad. Sci."},{"key":"2021042513312142600_JEB199190C34","doi-asserted-by":"publisher","first-page":"161","DOI":"10.1098\/rstb.1974.0024","article-title":"The structure of the stomatogastric neuromuscular system in Callinectes sapidus, Homarus americanus and Panulirus argus (decapoda crustacea)","volume":"268","author":"Maynard","year":"1974","journal-title":"Philos. Trans. R. Soc. Lond."},{"key":"2021042513312142600_JEB199190C35","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1016\/B978-012247570-2\/50040-2","article-title":"The physiology of gas exchange, circulation, ion regulation and nitrogenous excretion: an integrative approach","volume-title":"Biology of the Lobster Homarus americanus","author":"McMahon","year":"1995"},{"key":"2021042513312142600_JEB199190C36","doi-asserted-by":"publisher","first-page":"539","DOI":"10.1016\/S1095-6433(99)00147-6","article-title":"Intrinsic and extrinsic influences on cardiac rhythms in crustaceans","volume":"124","author":"McMahon","year":"1999","journal-title":"Comp. Biochem. Physiol. A Mol. Integr. Physiol."},{"key":"2021042513312142600_JEB199190C37","doi-asserted-by":"crossref","first-page":"923","DOI":"10.1242\/jeb.204.5.923","article-title":"Control of cardiovascular function and its evolution in Crustacea","volume":"204","author":"McMahon","year":"2001","journal-title":"J. Exp. Biol."},{"key":"2021042513312142600_JEB199190C38","doi-asserted-by":"publisher","first-page":"349","DOI":"10.1016\/S0034-5687(01)00311-5","article-title":"Respiratory and circulatory compensation to hypoxia in crustaceans","volume":"128","author":"McMahon","year":"2001","journal-title":"Respir. Physiol."},{"key":"2021042513312142600_JEB199190C39","doi-asserted-by":"publisher","first-page":"691","DOI":"10.1016\/S0006-3495(99)77236-X","article-title":"Analysis of dynamic brain imaging data","volume":"76","author":"Mitra","year":"1999","journal-title":"Biophys. J."},{"key":"2021042513312142600_JEB199190C40","doi-asserted-by":"publisher","first-page":"1345","DOI":"10.1038\/nn1352","article-title":"Similar network activity from disparate circuit parameters","volume":"7","author":"Prinz","year":"2004","journal-title":"Nat. Neurosci."},{"key":"2021042513312142600_JEB199190C41","doi-asserted-by":"publisher","first-page":"257","DOI":"10.1109\/5.18626","article-title":"A tutorial on hidden Markov models and selected applications in speech recognition","volume":"77","author":"Rabiner","year":"1989","journal-title":"Proc. IEEE"},{"key":"2021042513312142600_JEB199190C42","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1007\/BF00610338","article-title":"Expression of the crustacean pyloric pattern generator in the intact animal","volume":"153","author":"Rezer","year":"1983","journal-title":"J. Comp. Physiol. A"},{"key":"2021042513312142600_JEB199190C43","doi-asserted-by":"publisher","first-page":"e1002857","DOI":"10.1371\/journal.pcbi.1002857","article-title":"The effects of temperature on the stability of a neuronal oscillator","volume":"9","author":"Rinberg","year":"2013","journal-title":"PLoS Comput. Biol."},{"key":"2021042513312142600_JEB199190C44","doi-asserted-by":"publisher","first-page":"356","DOI":"10.1038\/nn1639","article-title":"Variable channel expression in identified single and electrically coupled neurons in different animals","volume":"9","author":"Schulz","year":"2006","journal-title":"Nat. Neurosci."},{"key":"2021042513312142600_JEB199190C45","doi-asserted-by":"publisher","first-page":"13187","DOI":"10.1073\/pnas.0705827104","article-title":"Quantitative expression profiling of identified neurons reveals cell-specific constraints on highly variable levels of gene expression","volume":"104","author":"Schulz","year":"2007","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2021042513312142600_JEB199190C46","doi-asserted-by":"publisher","first-page":"2603","DOI":"10.1152\/jn.00906.2013","article-title":"Phase maintenance in a rhythmic motor pattern during temperature changes in vivo","volume":"111","author":"Soofi","year":"2014","journal-title":"J. Neurophysiol."},{"key":"2021042513312142600_JEB199190C47","doi-asserted-by":"publisher","first-page":"173","DOI":"10.1002\/neu.480150302","article-title":"Dual effects of proctolin on the rhythmic burst activity of the cardiac ganglion","volume":"15","author":"Sullivan","year":"1984","journal-title":"J. Neurobiol."},{"key":"2021042513312142600_JEB199190C48","doi-asserted-by":"publisher","first-page":"e1000469","DOI":"10.1371\/journal.pbio.1000469","article-title":"Precise temperature compensation of phase in a rhythmic motor pattern","volume":"8","author":"Tang","year":"2010","journal-title":"PLoS Biol."},{"key":"2021042513312142600_JEB199190C49","doi-asserted-by":"publisher","first-page":"10075","DOI":"10.1523\/JNEUROSCI.1443-12.2012","article-title":"Robustness of a rhythmic circuit to short- and long-term temperature changes","volume":"32","author":"Tang","year":"2012","journal-title":"J. Neurosci."},{"key":"2021042513312142600_JEB199190C50","doi-asserted-by":"publisher","first-page":"1899","DOI":"10.1016\/j.cub.2014.06.067","article-title":"Activity-dependent feedback regulates correlated ion channel mRNA levels in single identified motor neurons","volume":"24","author":"Temporal","year":"2014","journal-title":"Curr. Biol."},{"key":"2021042513312142600_JEB199190C51","doi-asserted-by":"publisher","first-page":"16565","DOI":"10.1523\/JNEUROSCI.2903-13.2013","article-title":"The neuromuscular transform of the lobster cardiac system explains the opposing effects of a neuromodulator on muscle output","volume":"33","author":"Williams","year":"2013","journal-title":"J. Neurosci."},{"key":"2021042513312142600_JEB199190C52","doi-asserted-by":"publisher","first-page":"1024","DOI":"10.1242\/jeb.02082","article-title":"Temperature dependence of cardiac performance in the lobster Homarus americanus","volume":"209","author":"Worden","year":"2006","journal-title":"J. Exp. Biol."}],"container-title":["Journal of Experimental Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1242\/jeb.199190","content-type":"unspecified","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/journals.biologists.com\/jeb\/article-pdf\/doi\/10.1242\/jeb.199190\/1920050\/jeb199190.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/journals.biologists.com\/jeb\/article-pdf\/doi\/10.1242\/jeb.199190\/2043118\/jeb_199190v1.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"http:\/\/journals.biologists.com\/jeb\/article-pdf\/doi\/10.1242\/jeb.199190\/2043118\/jeb_199190v1.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,5,18]],"date-time":"2021-05-18T15:02:32Z","timestamp":1621350152000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.biologists.com\/jeb\/article\/doi\/10.1242\/jeb.199190\/259667\/In-vivo-effects-of-temperature-on-the-heart-and"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,1,1]]},"references-count":52,"URL":"https:\/\/doi.org\/10.1242\/jeb.199190","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/249631","asserted-by":"object"}]},"ISSN":["1477-9145","0022-0949"],"issn-type":[{"value":"1477-9145","type":"electronic"},{"value":"0022-0949","type":"print"}],"subject":[],"published":{"date-parts":[[2019,1,1]]},"article-number":"jeb.199190"}}