{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T08:40:34Z","timestamp":1769157634258,"version":"3.49.0"},"reference-count":30,"publisher":"The Company of Biologists","license":[{"start":{"date-parts":[[2019,1,1]],"date-time":"2019-01-01T00:00:00Z","timestamp":1546300800000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"funder":[{"DOI":"10.13039\/501100008982","name":"National Science Foundation","doi-asserted-by":"publisher","award":["IOS #1354181"],"award-info":[{"award-number":["IOS #1354181"]}],"id":[{"id":"10.13039\/501100008982","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005384","name":"American Physiological Society","doi-asserted-by":"publisher","award":["William Townsend Porter Predoctoral Fellowship"],"award-info":[{"award-number":["William Townsend Porter Predoctoral Fellowship"]}],"id":[{"id":"10.13039\/100005384","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>Na+\/K+-ATPase (NKA)- and vacuolar H+-ATPase (VHA)-rich cells in shark gills respectively secrete excess acid and base to seawater to maintain blood acid-base homeostasis. Both cell types are rich in mitochondria indicating high ATP demand; however, their metabolic fuel is unknown. Here we report that NKA- and VHA-rich cells have large glycogen stores. Glycogen abundance in NKA-rich cells was lower in starved sharks compared to 24h post-fed sharks, reflecting higher energy demand for acid secretion during normal activity and glycogen replenishment during the post-feeding period. Conversely, glycogen abundance in VHA-rich cells was high in starved sharks and it became depleted post-feeding. Furthermore, inactive cells with cytoplasmic VHA had large glycogen stores and active cells with basolateral VHA had depleted glycogen stores. These results indicate glycogen is a main energy source in both NKA- and VHA-rich cells, and point to differential energy use associated with net acid and net base secretion, respectively.<\/jats:p>","DOI":"10.1242\/jeb.199448","type":"journal-article","created":{"date-parts":[[2019,5,13]],"date-time":"2019-05-13T17:25:40Z","timestamp":1557768340000},"source":"Crossref","is-referenced-by-count":3,"title":["Differential glycogen utilization in shark acid- and base-regulatory gill cells"],"prefix":"10.1242","author":[{"given":"Jinae N.","family":"Roa","sequence":"first","affiliation":[{"name":"Marine Biology Research Division, Scripps Institution of Oceanography, University of California San Diego, 9500 Gilman Drive, La Jolla, California, 92093, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7090-9266","authenticated-orcid":false,"given":"Martin","family":"Tresguerres","sequence":"additional","affiliation":[{"name":"Marine Biology Research Division, Scripps Institution of Oceanography, University of California San Diego, 9500 Gilman Drive, La Jolla, California, 92093, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[2019,1,1]]},"reference":[{"key":"2021042523211298800_JEB199448C1","doi-asserted-by":"publisher","first-page":"264","DOI":"10.5357\/koubyou.60.264","article-title":"Production of monoclonal antibody that recognizes glycogen and its application for immunohistochemistry","volume":"60","author":"Baba","year":"1993","journal-title":"Kokubyo Gakkai Zasshi"},{"key":"2021042523211298800_JEB199448C2","doi-asserted-by":"publisher","first-page":"537","DOI":"10.1111\/j.1471-4159.2004.02421.x","article-title":"Brain glycogen re-awakened","volume":"89","author":"Brown","year":"2004","journal-title":"J. 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