{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T19:49:17Z","timestamp":1787946557175,"version":"build-2784847793"},"reference-count":53,"publisher":"The Company of Biologists","issue":"18","license":[{"start":{"date-parts":[[1998,9,15]],"date-time":"1998-09-15T00:00:00Z","timestamp":905817600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1998,9,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>We have examined the accumulative transport properties of the Malpighian (excretory) tubules of the tobacco hornworm Manduca sexta to test the hypothesis that a P-glycoprotein-like multidrug transporter is active and is responsible for the excretion of dietary nicotine in this tissue. Isolated tubules were cannulated and exposed to radiolabelled forms of either nicotine (5 min exposure) or the P-glycoprotein substrate vinblastine (60 min exposure) in the bathing (basal surface) fluid. The luminal (apical) contents were then flushed, and lumen-to-bath ratios were measured. Although these ratios provide conservative estimates of the physiological ability of Malpighian tubules to move compounds from blood to lumen, tubules concentrated nicotine 10-fold from an initial bath concentration of 0.5 mmol l\u22121 and vinblastine threefold (from an initial concentration of 1 \u03bcmol l\u22121).<\/jats:p>\n               <jats:p>Vectorial transport of vinblastine and nicotine was eliminated by 25 \u03bcmol l\u22121 verapamil (a P-glycoprotein inhibitor) and was not dependent on the presence of a transepithelial electrical potential. Nicotine transport was inhibited by atropine (3 mmol l\u22121), while nicotine (\u2a7e50 \u03bcmol l\u22121) significantly reduced vinblastine transport. Verapamil was effective at reducing vinblastine transport when applied to the basal side alone, but not when applied to the apical side alone. Taken together, these results are consistent with the idea that the active excretion of nicotine and other alkaloids by the tobacco hornworm is mediated by a P-glycoprotein-like mechanism.<\/jats:p>","DOI":"10.1242\/jeb.201.18.2637","type":"journal-article","created":{"date-parts":[[2021,4,23]],"date-time":"2021-04-23T23:27:57Z","timestamp":1619220477000},"page":"2637-2645","source":"Crossref","is-referenced-by-count":81,"title":["Transepithelial transport of nicotine and vinblastine in isolated malpighian tubules of the tobacco hornworm (<i>Manduca sexta<\/i>) suggests a P-glycoprotein-like mechanism"],"prefix":"10.1242","volume":"201","author":[{"given":"Lorin S.","family":"Gaertner","sequence":"first","affiliation":[{"name":"University of Ottawa , Department of Biology and Neurosciences , , Ottawa Hospital, 725 Parkdale Avenue, Ottawa, Ontario, Canada K1Y 4E9"},{"name":"Loeb Research Institute , Department of Biology and Neurosciences , , Ottawa Hospital, 725 Parkdale Avenue, Ottawa, Ontario, Canada K1Y 4E9"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine L.","family":"Murray","sequence":"additional","affiliation":[{"name":"University of Ottawa , Department of Biology and Neurosciences , , Ottawa Hospital, 725 Parkdale Avenue, Ottawa, Ontario, Canada K1Y 4E9"},{"name":"Loeb Research Institute , Department of Biology and Neurosciences , , Ottawa Hospital, 725 Parkdale Avenue, Ottawa, Ontario, Canada K1Y 4E9"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine E.","family":"Morris","sequence":"additional","affiliation":[{"name":"University of Ottawa , Department of Biology and Neurosciences , , Ottawa Hospital, 725 Parkdale Avenue, Ottawa, Ontario, Canada K1Y 4E9"},{"name":"Loeb Research Institute , Department of Biology and Neurosciences , , Ottawa Hospital, 725 Parkdale Avenue, Ottawa, Ontario, Canada K1Y 4E9"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[1998,9,15]]},"reference":[{"key":"2024110710410889200_JEXBIO_201_18_2637C1","first-page":"144","article-title":"Most drugs that reverse multidrug resistance also inhibit photoaffinity labeling of P-glycoprotein by a vinblastine analog","volume":"33","author":"Akiyama","year":"1988","journal-title":"Mol. 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