{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,12,19]],"date-time":"2024-12-19T05:09:22Z","timestamp":1734584962309,"version":"3.30.2"},"reference-count":36,"publisher":"The Company of Biologists","issue":"19","license":[{"start":{"date-parts":[[2000,10,1]],"date-time":"2000-10-01T00:00:00Z","timestamp":970358400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2000,10,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>The blood serum of the European flounder Platichthys flesus strongly inhibits soluble erythrocytic carbonic anhydrase from the same species. The inhibition is of the uncompetitive type. Hence, the mechanism of the carbonic anhydrase inhibition is different from that of all other known carbonic anhydrase inhibitors. The serum showed no inhibitory effect on carbonic anhydrase from human and bovine red blood cells. By applying the 18O exchange reaction, it could be demonstrated that the presence of the carbonic anhydrase inhibitor in the extracellular fluid has no effect on carbonic anhydrase in intact red blood cells. Thus, this carbonic anhydrase inhibitor seems to act only within the plasma space of the circulatory system.<\/jats:p>\n               <jats:p>However, the carbonic anhydrase inhibitor does appear to reduce the bicarbonate permeability of flounder red cells to approximately one-quarter of normal levels as measured by the 18O exchange reaction. The 28 kDa carbonic anhydrase inhibitor was isolated from the serum by gel filtration. The isolated inhibitor was detected in acrylamide gels as a single band representing a 7 kDa protein. The denaturing conditions used in electrophoresis presumably led to a dissociation of the native protein into subunits.<\/jats:p>","DOI":"10.1242\/jeb.203.19.3003","type":"journal-article","created":{"date-parts":[[2021,4,25]],"date-time":"2021-04-25T04:37:39Z","timestamp":1619325459000},"page":"3003-3009","source":"Crossref","is-referenced-by-count":8,"title":["Properties of a Carbonic Anhydrase Inhibitor Protein in Flounder Serum"],"prefix":"10.1242","volume":"203","author":[{"given":"Thomas","family":"Peters","sequence":"first","affiliation":[{"name":"Zentrum Physiologie, Medizinische Hochschule Hannover, Carl-Neuberg-Strasse 1, D-30623 Hannover, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frauke","family":"Papadopoulos","sequence":"additional","affiliation":[{"name":"Zentrum Physiologie, Medizinische Hochschule Hannover, Carl-Neuberg-Strasse 1, D-30623 Hannover, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hans-Peter","family":"Kubis","sequence":"additional","affiliation":[{"name":"Zentrum Physiologie, Medizinische Hochschule Hannover, Carl-Neuberg-Strasse 1, D-30623 Hannover, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gerolf","family":"Gros","sequence":"additional","affiliation":[{"name":"Zentrum Physiologie, Medizinische Hochschule Hannover, Carl-Neuberg-Strasse 1, D-30623 Hannover, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[2000,10,1]]},"reference":[{"key":"2024121805084289900_JEXBIO_203_19_3003C1","doi-asserted-by":"publisher","first-page":"449","DOI":"10.1111\/j.1748-1716.1989.tb08776.x","article-title":"Tissue distribution of 125I-labelled carbonic anhydrase isozymes I, II and II in the rat","volume":"137","author":"Appelgren","year":"1989","journal-title":"Acta Physiol. Scand"},{"key":"2024121805084289900_JEXBIO_203_19_3003C2","doi-asserted-by":"publisher","first-page":"679","DOI":"10.1111\/j.1469-7793.1997.679bj.x","article-title":"Mass spectrometric determination of HCO3\u2212 permeability and carbonic anhydrase activity in intact guinea-pig colon epithelium","volume":"502","author":"B\u00f6llert","year":"1997","journal-title":"J. Physiol., Lond"},{"key":"2024121805084289900_JEXBIO_203_19_3003C3","doi-asserted-by":"publisher","first-page":"474","DOI":"10.1113\/jphysiol.1938.sp003574","article-title":"The carbonic anhydrase inhibitor in serum","volume":"91","author":"Booth","year":"1938","journal-title":"J. Physiol., Lond"},{"key":"2024121805084289900_JEXBIO_203_19_3003C4","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1007\/978-1-4899-0750-9_9","article-title":"Modified micromethod for assay of carbonic anhydrase activity","volume-title":"In The Carbonic Anhydrases: Cellular Physiology and Molecular Genetics","author":"Bruns","year":"1991"},{"key":"2024121805084289900_JEXBIO_203_19_3003C5","doi-asserted-by":"publisher","first-page":"812","DOI":"10.1039\/j19660000812","article-title":"The catalysis of the reaction between carbon dioxide and water","volume":"1966","author":"Dennard","year":"1966","journal-title":"J. Chem. Soc. A"},{"key":"2024121805084289900_JEXBIO_203_19_3003C6","doi-asserted-by":"publisher","first-page":"381","DOI":"10.1007\/BF00004302","article-title":"The carbonic anhydrase inhibitor in trout plasma: purification and its effect on carbonic anhydrase activity and the Root effect","volume":"12","author":"Dimberg","year":"1994","journal-title":"Fish Physiol. Biochem"},{"key":"2024121805084289900_JEXBIO_203_19_3003C7","first-page":"449","article-title":"Extracellular carbonic anhydrase and blood pH equilibrium in exercising skeletal muscle","volume":"433","author":"Geers","year":"1997","journal-title":"Pfl\u00fcgers Arch. (Suppl"},{"key":"2024121805084289900_JEXBIO_203_19_3003C8","doi-asserted-by":"publisher","first-page":"681","DOI":"10.1152\/physrev.2000.80.2.681","article-title":"CO2 transport and carbonic anhydrase in blood and muscle","volume":"80","author":"Geers","year":"2000","journal-title":"Physiol. Rev"},{"key":"2024121805084289900_JEXBIO_203_19_3003C9","doi-asserted-by":"publisher","first-page":"175","DOI":"10.1016\/0300-9629(83)90731-4","article-title":"An investigation of the carbonic anhydrase inhibitor in eel plasma","volume":"74A","author":"Haswell","year":"1983","journal-title":"Comp. Biochem. Physiol"},{"key":"2024121805084289900_JEXBIO_203_19_3003C10","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1016\/0034-5687(76)90082-7","article-title":"Carbonic anhydrase inhibitor in trout plasma","volume":"28","author":"Haswell","year":"1976","journal-title":"Respir. Physiol"},{"key":"2024121805084289900_JEXBIO_203_19_3003C11","doi-asserted-by":"publisher","first-page":"1642","DOI":"10.1152\/jappl.1993.75.4.1642","article-title":"Inhibitory sensitivity of pulmonary vascular carbonic anhydrase","volume":"75","author":"Heming","year":"1993","journal-title":"J. Appl. Physiol"},{"key":"2024121805084289900_JEXBIO_203_19_3003C12","doi-asserted-by":"publisher","first-page":"650","DOI":"10.1086\/515878","article-title":"Extracellular carbonic anhydrase activity and carbonic anhydrase inhibitors in the circulatory system of fish","volume":"70","author":"Henry","year":"1997","journal-title":"Physiol. Zool"},{"key":"2024121805084289900_JEXBIO_203_19_3003C13","doi-asserted-by":"publisher","first-page":"201","DOI":"10.1242\/jeb.134.1.201","article-title":"The distribution of branchial carbonic anhydrase and the effects of gill and erythrocyte carbonic anhydrase inhibition in the channel catfish Ictalurus punctatus","volume":"134","author":"Henry","year":"1988","journal-title":"J. Exp. Biol"},{"key":"2024121805084289900_JEXBIO_203_19_3003C14","doi-asserted-by":"publisher","first-page":"380","DOI":"10.1007\/BF00265642","article-title":"The distribution of carbonic anhydrase type I and II isozymes in lamprey and trout: possible co-evolution with erythrocyte chloride\/bicarbonate exchange","volume":"163","author":"Henry","year":"1993","journal-title":"J. Comp. Physiol. B"},{"key":"2024121805084289900_JEXBIO_203_19_3003C15","first-page":"R1754","article-title":"Carbonic anhydrase facilitates CO2 and NH3 transport across the sarcolemma of trout white muscle","volume":"272","author":"Henry","year":"1997","journal-title":"Am. J. Physiol"},{"key":"2024121805084289900_JEXBIO_203_19_3003C16","doi-asserted-by":"publisher","first-page":"103","DOI":"10.1002\/elps.1150060302","article-title":"Simplified method for silver staining of proteins in polyacrylamide gels and the mechanism of silver staining","volume":"6","author":"Heukeshoven","year":"1985","journal-title":"Electrophoresis"},{"key":"2024121805084289900_JEXBIO_203_19_3003C17","doi-asserted-by":"publisher","first-page":"191","DOI":"10.1152\/jappl.1986.60.1.191","article-title":"Inhibition of carbonic anhydrase by plasma of dogs and rabbits","volume":"60","author":"Hill","year":"1986","journal-title":"J. Appl. Physiol"},{"key":"2024121805084289900_JEXBIO_203_19_3003C18","doi-asserted-by":"publisher","first-page":"3881","DOI":"10.1016\/S0021-9258(17)40334-6","article-title":"Carbonic anhydrase activity in intact red blood cells measured with 18O exchange","volume":"252","author":"Itada","year":"1977","journal-title":"J. Biol. Chem"},{"key":"2024121805084289900_JEXBIO_203_19_3003C19","doi-asserted-by":"publisher","first-page":"625","DOI":"10.1146\/annurev.ph.50.030188.003205","article-title":"Velocity of CO2 exchange in blood","volume":"50","author":"Klocke","year":"1988","journal-title":"Annu. Rev. Physiol"},{"key":"2024121805084289900_JEXBIO_203_19_3003C20","doi-asserted-by":"publisher","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during the assembly of the head of bacteriophage T4","volume":"227","author":"Laemmli","year":"1970","journal-title":"Nature"},{"key":"2024121805084289900_JEXBIO_203_19_3003C21","doi-asserted-by":"publisher","first-page":"144","DOI":"10.1515\/bchm2.1962.327.1.144","article-title":"\u00dcber die Kohlens\u00e4ure-Dehydratase in den einzelnen Wirbeltierklassen. Der Zinkgehalt in den einzelnen Fermenten und die Wirkung des Inhibitors aus dem Schafblut auf die einzelnen Enzyme","volume":"327","author":"Leiner","year":"1962","journal-title":"Hoppe Seyler Physiol. Chem"},{"key":"2024121805084289900_JEXBIO_203_19_3003C22","first-page":"447","article-title":"Le dosage de l\u2019anhydrase carbonique. \u00c9tude de quelques substances inhibitrices et activatrices","volume":"38","author":"Maetz","year":"1956","journal-title":"Bull. Soc. Chim. Biol"},{"key":"2024121805084289900_JEXBIO_203_19_3003C23","first-page":"26","article-title":"A simplified micromethod for the determination of carbonic anhydrase and its inhibitors","volume":"130","author":"Maren","year":"1960","journal-title":"J. Pharmac. Exp. Ther"},{"key":"2024121805084289900_JEXBIO_203_19_3003C24","doi-asserted-by":"publisher","first-page":"595","DOI":"10.1152\/physrev.1967.47.4.595","article-title":"Carbonic anhydrase: chemistry, physiology and inhibition","volume":"47","author":"Maren","year":"1967","journal-title":"Physiol. Rev"},{"key":"2024121805084289900_JEXBIO_203_19_3003C25","doi-asserted-by":"publisher","first-page":"426","DOI":"10.1016\/0003-9861(84)90289-3","article-title":"Isolation, characterization and esterase and CO2 hydration activities of ubiquitin from bovine erythrocytes","volume":"234","author":"Matsumoto","year":"1984","journal-title":"Arch. Biochem. Biophys"},{"key":"2024121805084289900_JEXBIO_203_19_3003C26","doi-asserted-by":"publisher","first-page":"531","DOI":"10.1111\/j.1748-1716.1994.tb09776.x","article-title":"Renal handling and plasma elimination kinetics of carbonic anhydrase isoenzymes I, II and III in the rat","volume":"151","author":"\u00d6jteg","year":"1994","journal-title":"Acta Physiol. Scand"},{"key":"2024121805084289900_JEXBIO_203_19_3003C27","doi-asserted-by":"publisher","first-page":"1551","DOI":"10.1242\/jeb.203.10.1551","article-title":"Hagfish (Myxine glutinosa) red cell membrane exhibits no bicarbonate permeability as detected by 18O exchange","volume":"203","author":"Peters","year":"2000","journal-title":"J. Exp. Biol"},{"key":"2024121805084289900_JEXBIO_203_19_3003C28","doi-asserted-by":"publisher","first-page":"344","DOI":"10.1007\/BF00589244","article-title":"Inhibition of carbonic anhydrase in dog plasma","volume":"403","author":"Rispens","year":"1985","journal-title":"Pfl\u00fcgers Arch"},{"key":"2024121805084289900_JEXBIO_203_19_3003C29","doi-asserted-by":"publisher","first-page":"12536","DOI":"10.1021\/bi00164a034","article-title":"Purification and characterization of a carbonic anhydrase II inhibitor from porcine plasma","volume":"31","author":"Roush","year":"1992","journal-title":"Biochemistry"},{"key":"2024121805084289900_JEXBIO_203_19_3003C30","doi-asserted-by":"publisher","first-page":"43","DOI":"10.1177\/002215549904700105","article-title":"Carbonic anhydrase in the gills of seawater- and freshwater-acclimated flounders Platichthys flesus: Purification, characterization and immunohistochemical localization","volume":"47","author":"Sender","year":"1999","journal-title":"J. Histochem. Cytochem"},{"key":"2024121805084289900_JEXBIO_203_19_3003C31","doi-asserted-by":"publisher","first-page":"855","DOI":"10.1177\/002215549804600709","article-title":"Localization of carbonic anhydrase IV in rat and human heart muscle","volume":"46","author":"Sender","year":"1998","journal-title":"J. Histochem. Cytochem"},{"key":"2024121805084289900_JEXBIO_203_19_3003C32","doi-asserted-by":"publisher","first-page":"375","DOI":"10.1146\/annurev.bi.64.070195.002111","article-title":"Human carbonic anhydrases and carbonic anhydrase deficiencies","volume":"64","author":"Sly","year":"1995","journal-title":"Annu. Rev. Biochem"},{"key":"2024121805084289900_JEXBIO_203_19_3003C33","first-page":"17","article-title":"Effect of membrane-bound carbonic anhydrase (CA) inhibition on bicarbonate excretion in the shark Squalus acanthias","volume":"35","author":"Swenson","year":"1996","journal-title":"Bull. Mt Desert Island Biol. Lab"},{"key":"2024121805084289900_JEXBIO_203_19_3003C34","doi-asserted-by":"publisher","first-page":"151","DOI":"10.1006\/abbi.1998.0762","article-title":"Inhibition and kinetic properties of membrane-bound carbonic anhydrases in rabbit skeletal muscles","volume":"356","author":"Wetzel","year":"1998","journal-title":"Arch. Biochem. Biophys"},{"key":"2024121805084289900_JEXBIO_203_19_3003C35","doi-asserted-by":"publisher","first-page":"4327","DOI":"10.1021\/bi9627424","article-title":"Cloning, sequencing and recombinant expression of the porcine inhibitor of carbonic anhydrase: A novel member of the transferrin family","volume":"36","author":"Wuebbens","year":"1997","journal-title":"Biochemistry"},{"key":"2024121805084289900_JEXBIO_203_19_3003C36","doi-asserted-by":"publisher","first-page":"303","DOI":"10.1080\/10256019808234064","article-title":"18O exchange in suspensions of red blood cells: Determination of parameters of mass spectrometer inlet system","volume":"34","author":"Wunder","year":"1998","journal-title":"Isotopes Env. Health Stud"}],"container-title":["Journal of Experimental Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.biologists.com\/jeb\/article-pdf\/203\/19\/3003\/3602792\/jexbio_203_19_3003.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.biologists.com\/jeb\/article-pdf\/203\/19\/3003\/3602792\/jexbio_203_19_3003.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,18]],"date-time":"2024-12-18T05:08:54Z","timestamp":1734498534000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.biologists.com\/jeb\/article\/203\/19\/3003\/8604\/Properties-of-a-Carbonic-Anhydrase-Inhibitor"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,10,1]]},"references-count":36,"journal-issue":{"issue":"19","published-print":{"date-parts":[[2000,10,1]]}},"URL":"https:\/\/doi.org\/10.1242\/jeb.203.19.3003","relation":{},"ISSN":["0022-0949","1477-9145"],"issn-type":[{"type":"print","value":"0022-0949"},{"type":"electronic","value":"1477-9145"}],"subject":[],"published":{"date-parts":[[2000,10,1]]}}}