{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T00:53:01Z","timestamp":1790902381697,"version":"4.1.0"},"reference-count":29,"publisher":"The Company of Biologists","issue":"10","license":[{"start":{"date-parts":[[2001,5,15]],"date-time":"2001-05-15T00:00:00Z","timestamp":989884800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2001,5,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>The Drosophila melanogaster homologue of an insect calcitonin-like diuretic hormone was identified in a BLAST search of the Drosophila genome database. The predicted 31-residue amidated peptide (D. melanogaster DH31; Drome-DH31) was synthesised and tested for activity on fruit fly Malpighian tubules. It increases tubule secretion by approximately 35 % of the response obtained with a myokinin from the housefly Musca domestica (muscakinin; Musdo-K) and has an EC50 of 4.3 nmol l\u22121.<\/jats:p>\n               <jats:p>The diuretic activities of Drome-DH31 and Musdo-K were additive when tested at threshold and supra-maximal concentrations, which suggests that they target different transport processes. In support of this, Drome-DH31 increased the rate of secretion by tubules held in bathing fluid with a reduced Cl\u2212 concentration, whereas Musdo-K did so only in the presence of Drome-DH31. Stimulation with Drome-DH31 increased the lumen-positive transepithelial potential in the main secretory segment of the tubule. This was attributed to activation of an apical electrogenic proton-translocating V-ATPase in principal cells, since it was associated with hyperpolarisation of the apical membrane potential and acidification of secreted urine by 0.25 pH units.<\/jats:p>\n               <jats:p>Exogenous 8-bromo-cyclic AMP and cyclic GMP increased tubule secretion to the same extent as Drome-DH31 and, when tested together with the diuretic peptide, their activities were not additive. Stimulation with Drome-DH31 resulted in a dose-dependent increase in cyclic AMP production by tubules incubated in saline containing 0.5 mmol l\u22121 3-isobutyl-1-methylxanthine, whereas cyclic GMP production was unchanged. Taken together, the data are consistent with Drome-DH31 activating an apical membrane V-ATPase via cyclic AMP. Since the K+ concentration of the secreted urine was unchanged, it is likely that Drome-DH31 has an equal effect on K+ and Na+ entry across the basolateral membrane.<\/jats:p>","DOI":"10.1242\/jeb.204.10.1795","type":"journal-article","created":{"date-parts":[[2021,4,23]],"date-time":"2021-04-23T23:48:54Z","timestamp":1619221734000},"page":"1795-1804","source":"Crossref","is-referenced-by-count":154,"title":["The <i>Drosophila Melanogaster<\/i> Homologue of an Insect Calcitonin-Like Diuretic Peptide Stimulates V-ATPase Activity in Fruit Fly Malpighian Tubules"],"prefix":"10.1242","volume":"204","author":[{"given":"Geoffrey M.","family":"Coast","sequence":"first","affiliation":[{"name":"1 Department of Biology, Birkbeck College, Malet Street, London WC1E 7HX, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon G.","family":"Webster","sequence":"additional","affiliation":[{"name":"School of Biological Sciences 2 , University of North Wales, Gwynedd LL57 2UW, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathleen M.","family":"Schegg","sequence":"additional","affiliation":[{"name":"3 Department of Biochemistry, University of Nevada, Reno, NV 89557, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen S.","family":"Tobe","sequence":"additional","affiliation":[{"name":"4 Department of Zoology, University of Toronto, Ontario, Canada M55 3G5"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David A.","family":"Schooley","sequence":"additional","affiliation":[{"name":"3 Department of Biochemistry, University of Nevada, Reno, NV 89557, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2001,5,15]]},"reference":[{"key":"2024121805265811100_JEXBIO_204_10_1795C1","doi-asserted-by":"publisher","first-page":"2185","DOI":"10.1126\/science.287.5461.2185","article-title":"The genome sequence of Drosophila melanogaster","volume":"287","author":"Adams","year":"2000","journal-title":"Science"},{"key":"2024121805265811100_JEXBIO_204_10_1795C2","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1016\/B978-0-12-185150-7.50032-4","article-title":"Comparative methods for the radiolabelling of peptides","volume-title":"In Neuroendocrine Peptide Methodology","author":"Bolton","year":"1989"},{"key":"2024121805265811100_JEXBIO_204_10_1795C3","first-page":"1","article-title":"Radioimmunoassy of cyclic AMP and cyclic GMP","volume-title":"In Advances in Cyclic Nucleotide Research","author":"Brooker","year":"1979"},{"key":"2024121805265811100_JEXBIO_204_10_1795C4","doi-asserted-by":"publisher","first-page":"283","DOI":"10.1016\/0167-0115(95)00042-A","article-title":"Synergism between diuretic peptides controlling ion and fluid transport in insect Malpighian tubules","volume":"57","author":"Coast","year":"1995","journal-title":"Regul. 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