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Control trout were treated identically but without exposure to ammonia. Ammonia exposure caused an increase in plasma total ammonia level to 436\u00b134\u03bcmoll\u22121, compared to 183\u00b130\u03bcmoll\u22121in control animals (N=6). A significant reduction in total plasma ammonia level was found in both groups during exercise, despite a large negative concentration gradient in those exposed to an elevated concentration of ammonia in water, which may indicate an active excretory process. The overall increase in plasma ammonia levels in exposed trout was associated with a significant reduction in critical swimming speed (Ucrit) to 1.61\u00b10.17BLs\u22121 from 2.23\u00b10.15BLs\u22121 in control animals. Ammonia-exposed trout had a significantly higher maintenance metabolic rate (MMR) than control fish, when estimated as the y-intercept of the relationship between swimming speed and MO2. Active metabolic rate (AMR, maximum MO2 as measured at Ucrit) was significantly lower in ammonia-exposed animals, leading to a profound reduction in factorial aerobic scope (AMR\/MMR). Reduced Ucrit was also linked to a reduction in maximum tailbeat frequency. Calculation of membrane potentials (EM) in the white muscle of fish swum to Ucrit revealed a significant partial depolarisation of white muscle in ammonia-exposed fish. This may have prevented white muscle recruitment and contributed to the reduced maximum tailbeat frequency and overall impairment of swimming performance in the ammonia-exposed fish.<\/jats:p>","DOI":"10.1242\/jeb.204.15.2691","type":"journal-article","created":{"date-parts":[[2021,4,23]],"date-time":"2021-04-23T23:40:10Z","timestamp":1619221210000},"page":"2691-2698","source":"Crossref","is-referenced-by-count":90,"title":["Effects of sublethal ammonia exposure on swimming performance in rainbow trout (<i>Oncorhynchus mykiss<\/i>)"],"prefix":"10.1242","volume":"204","author":[{"given":"A.","family":"Shingles","sequence":"first","affiliation":[{"name":"School of Biosciences, University of Birmingham, Birmingham, B15 2TT, UK and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. 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