{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T05:57:48Z","timestamp":1791179868445,"version":"4.1.0"},"reference-count":47,"publisher":"The Company of Biologists","issue":"7","license":[{"start":{"date-parts":[[2001,4,1]],"date-time":"2001-04-01T00:00:00Z","timestamp":986083200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2001,4,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Catecholamines and pro-opiomelanocortin (POMC)-derived peptides, some of the central regulators of the stress-response systems of vertebrates, are also present in invertebrates. However, studies are needed to determine how these hormones participate in the organisation of neuroendocrine stress-response axes in invertebrates. Our present work provides evidence for the presence of an adrenergic stress-response system in the oyster Crassostrea gigas. Noradrenaline and dopamine are released into the circulation in response to stress. Storage and release of these hormones take place in neurosecretory cells presenting morphological and biochemical similarities with vertebrate chromaffin cells. Both in vivo and in vitro experiments showed that applications of the neurotransmitters acetylcholine or carbachol caused no significant release of noradrenaline or dopamine. Moreover, the nicotinic antagonists hexamethonium and \u03b1-bungarotoxin and the muscarinic antagonist atropine caused no significant inhibition of catecholamine release in stressed oysters. Adrenocorticotropic hormone (ACTH) induced a significant release of noradrenaline, but the release of dopamine in response to ACTH was not significant. These results suggest that, unlike that of vertebrates, the adrenergic stress-response system of oysters is not under the control of acetylcholine and that other factors, such as the neuropeptide ACTH, might control this system.<\/jats:p>","DOI":"10.1242\/jeb.204.7.1247","type":"journal-article","created":{"date-parts":[[2021,4,25]],"date-time":"2021-04-25T00:43:39Z","timestamp":1619311419000},"page":"1247-1255","source":"Crossref","is-referenced-by-count":80,"title":["Evidence for a form of adrenergic response to stress in the mollusc\n                    <i>Crassostrea gigas<\/i>"],"prefix":"10.1242","volume":"204","author":[{"given":"A.","family":"Lacoste","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. K.","family":"Malham","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Pierre et Marie Curie, Paris 6, BP 74, F-29682 ROSCOFF, France Station Biologique de Roscoff, CNRS, INSU ,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Cueff","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Pierre et Marie Curie, Paris 6, BP 74, F-29682 ROSCOFF, France Station Biologique de Roscoff, CNRS, INSU ,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Jalabert","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Pierre et Marie Curie, Paris 6, BP 74, F-29682 ROSCOFF, France Station Biologique de Roscoff, CNRS, INSU ,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"G\u00e9l\u00e9bart","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Pierre et Marie Curie, Paris 6, BP 74, F-29682 ROSCOFF, France Station Biologique de Roscoff, CNRS, INSU ,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. A.","family":"Poulet","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Pierre et Marie Curie, Paris 6, BP 74, F-29682 ROSCOFF, France Station Biologique de Roscoff, CNRS, INSU ,"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2001,4,1]]},"reference":[{"key":"2024121805234764200_JEXBIO_204_7_1247C1","first-page":"319","article-title":"Diferentes localiza\u00e7\u00f5es dos paraganglios interatriais no cora\u00e7\u00e3o humano adulto","volume":"46","author":"Almeida","year":"1985","journal-title":"Arq. Bras. Cardiol."},{"key":"2024121805234764200_JEXBIO_204_7_1247C2","doi-asserted-by":"publisher","first-page":"81","DOI":"10.1007\/BF00571433","article-title":"Monoamines in the nervous system of the tube-worm Chaetopterus variopedatus (Polychaeta): Biochemical detection and serotonin immunoreactivity","volume":"259","author":"Anctil","year":"1990","journal-title":"Cell Tissue Res"},{"key":"2024121805234764200_JEXBIO_204_7_1247C3","doi-asserted-by":"publisher","first-page":"549","DOI":"10.1016\/0145-305X(88)90071-7","article-title":"Separation of Crassostrea gigas hemocytes by density gradient centrifugation and counterflow centrifugal elutriation","volume":"12","author":"Bach\u00e8re","year":"1988","journal-title":"Dev. Comp. Immunol"},{"key":"2024121805234764200_JEXBIO_204_7_1247C4","doi-asserted-by":"publisher","first-page":"2485","DOI":"10.1242\/jeb.199.11.2485","article-title":"Control of catecholamine and serotonin release from the chromaffin tissue of the Atlantic hagfish","volume":"199","author":"Bernier","year":"1996","journal-title":"J. Exp. Biol."},{"key":"2024121805234764200_JEXBIO_204_7_1247C5","first-page":"49","article-title":"Serotonin and dopamine: distribution and accumulation in Aplysia nervous and non-nervous tissues","volume":"2","author":"Carpenter","year":"1971","journal-title":"J. Neurosci."},{"key":"2024121805234764200_JEXBIO_204_7_1247C6","doi-asserted-by":"publisher","first-page":"1244","DOI":"10.1001\/jama.1992.03480090092034","article-title":"The concepts of stress and stress system disorders. Overview of physical and behavioural homeostasis","volume":"267","author":"Chrousos","year":"1992","journal-title":"J. Am. Med. Assoc."},{"key":"2024121805234764200_JEXBIO_204_7_1247C7","doi-asserted-by":"publisher","first-page":"109","DOI":"10.1007\/BF00754613","article-title":"Identification of media supplements that improve the viability of primarily cell cultures of Crassostrea gigas oysters","volume":"16","author":"Domart-Coulon","year":"1994","journal-title":"Cytotechnol."},{"key":"2024121805234764200_JEXBIO_204_7_1247C8","first-page":"271","article-title":"The circulatory system","volume-title":"The Eastern Oyster Crassostrea virginica","author":"Eble","year":"1996"},{"key":"2024121805234764200_JEXBIO_204_7_1247C9","doi-asserted-by":"publisher","first-page":"159","DOI":"10.1007\/BF00492292","article-title":"Water-stable fluorophores, produced by reaction with aldehyde solutions, for the histochemical localization of catechol- and indolethylamines","volume":"52","author":"Furness","year":"1977","journal-title":"Histochem."},{"key":"2024121805234764200_JEXBIO_204_7_1247C10","first-page":"13","article-title":"Cholinergic mechanisms in the neurocontrol of the branchial heart of the cephalopod Sepia officinalis L","volume":"119C","author":"Gebauer","year":"1998","journal-title":"Comp. Biochem. Physiol."},{"key":"2024121805234764200_JEXBIO_204_7_1247C11","doi-asserted-by":"publisher","first-page":"337","DOI":"10.1007\/BF00266960","article-title":"Immunocytochemical identification of neuroendocrine markers in human cardiac paraganglion-like structures","volume":"95","author":"Gobbi","year":"1991","journal-title":"Histochem."},{"key":"2024121805234764200_JEXBIO_204_7_1247C12","doi-asserted-by":"publisher","first-page":"467","DOI":"10.1016\/0024-3205(81)90139-9","article-title":"Validity and reliability of liquid chromatography with electrochemical detection for measuring plasma levels of noradrenaline and epinephrine in man","volume":"28","author":"Goldstein","year":"1981","journal-title":"Life Sci."},{"key":"2024121805234764200_JEXBIO_204_7_1247C13","doi-asserted-by":"publisher","first-page":"498","DOI":"10.1007\/BF00928417","article-title":"Localisation and identification of catecholamines in the nervous system of Diphyllobothrium dendriticum (Cestoda)","volume":"77","author":"Gustafsson","year":"1991","journal-title":"Parasitol. Res."},{"key":"2024121805234764200_JEXBIO_204_7_1247C14","doi-asserted-by":"publisher","first-page":"1203","DOI":"10.1097\/00003246-198911000-00021","article-title":"Catecholamines: Study of interspecies variation","volume":"17","author":"Hart","year":"1989","journal-title":"Crit. Care Med."},{"key":"2024121805234764200_JEXBIO_204_7_1247C15","first-page":"999","article-title":"Neuromuscular transmission in molluscan hearts","volume":"7","author":"Hill","year":"1990","journal-title":"Zool. Sci."},{"key":"2024121805234764200_JEXBIO_204_7_1247C16","first-page":"280","article-title":"The distribution of biogenic amines in invertebrates","volume-title":"Neurobiology, Current Comparative Approaches","author":"Klemm","year":"1985"},{"key":"2024121805234764200_JEXBIO_204_7_1247C17","doi-asserted-by":"publisher","first-page":"2086","DOI":"10.1073\/pnas.79.6.2086","article-title":"Corticotropin and \u03b2-endorphin-like materials are native to unicellular organisms","volume":"79","author":"LeRoith","year":"1982","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2024121805234764200_JEXBIO_204_7_1247C18","first-page":"277","article-title":"Noncholinergic control of adrenal catecholamine secretion","volume":"183","author":"Livett","year":"1993","journal-title":"J. Anat."},{"key":"2024121805234764200_JEXBIO_204_7_1247C19","doi-asserted-by":"publisher","first-page":"14184","DOI":"10.1073\/pnas.95.24.14184","article-title":"Unmasking the functions of the chromaffin cell \u03b17nicotinic receptor by using short pulses of acetylcholine and selective blockers","volume":"95","author":"Lopez","year":"1998","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2024121805234764200_JEXBIO_204_7_1247C20","first-page":"171","article-title":"Seasonal variations of catecholamine levels in the tissues of the Japanese oyster, Crassostrea gigas","volume":"93C","author":"Osada","year":"1989","journal-title":"Comp. Biochem. Physiol."},{"key":"2024121805234764200_JEXBIO_204_7_1247C21","doi-asserted-by":"publisher","first-page":"446","DOI":"10.1006\/bbrc.1993.1644","article-title":"CRF provokes the release of norepinephrine by hemocytes of Viviparus ater (Gastropoda, Prosobranchia): further evidence in favour of the evolutionary hypothesis of the \u2018mobile immune brain\u2019","volume":"193","author":"Ottaviani","year":"1993","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"2024121805234764200_JEXBIO_204_7_1247C22","doi-asserted-by":"publisher","first-page":"215","DOI":"10.1098\/rspb.1991.0112","article-title":"ACTH-like molecules in gastropod molluscs: a possible role in ancestral immune response and stress","volume":"245","author":"Ottaviani","year":"1991","journal-title":"Proc. R. Soc. Lond. B"},{"key":"2024121805234764200_JEXBIO_204_7_1247C23","doi-asserted-by":"publisher","first-page":"421","DOI":"10.1016\/0301-0082(95)00049-6","article-title":"The neuroendocrinology of stress from invertebrates to man","volume":"48","author":"Ottaviani","year":"1996","journal-title":"Prog. Neurobiol."},{"key":"2024121805234764200_JEXBIO_204_7_1247C24","doi-asserted-by":"publisher","first-page":"139","DOI":"10.1007\/s000180050133","article-title":"In situ localization of ACTH receptor-like mRNA in molluscan and human immunocyte","volume":"54","author":"Ottaviani","year":"1998","journal-title":"Cell. Mol. Life Sci."},{"key":"2024121805234764200_JEXBIO_204_7_1247C25","doi-asserted-by":"publisher","first-page":"R1247","DOI":"10.1152\/ajpcell.1998.275.5.C1247","article-title":"Heterogeneous neurochemical responses to different stressors: a test of Selye\u2019s doctrine of nonspecificity","volume":"275","author":"Pacak","year":"1998","journal-title":"Am. J. Physiol."},{"key":"2024121805234764200_JEXBIO_204_7_1247C26","first-page":"161","article-title":"Quantitative survey of biogenic amines, their precursors and metabolites in the coelenterate Renilla koellikeri","volume":"10","author":"Pani","year":"1994","journal-title":"Biogenic Amines"},{"key":"2024121805234764200_JEXBIO_204_7_1247C27","doi-asserted-by":"publisher","first-page":"371","DOI":"10.1007\/BF02089947","article-title":"Distribution of catecholamines, indoleamines and their precursors and metabolites in the scallop, Placopecten magellanicus (Bivalvia, Pectinidae)","volume":"15","author":"Pani","year":"1995","journal-title":"Cell. Mol. Neurobiol."},{"key":"2024121805234764200_JEXBIO_204_7_1247C28","doi-asserted-by":"publisher","first-page":"165","DOI":"10.1242\/jeb.183.1.165","article-title":"Storage and release of catecholamines from the chromaffin tissue of the atlantic hagfish Myxine glutinosa","volume":"183","author":"Perry","year":"1993","journal-title":"J. Exp. Biol."},{"key":"2024121805234764200_JEXBIO_204_7_1247C29","first-page":"255","article-title":"Catecholamines in fish","volume-title":"Fish Physiology, vol. 12B","author":"Randall","year":"1992"},{"key":"2024121805234764200_JEXBIO_204_7_1247C30","first-page":"1","article-title":"The adrenergic response in fish: control of catecholamine storage and release","volume":"120C","author":"Reid","year":"1998","journal-title":"Comp. Biochem. Physiol."},{"key":"2024121805234764200_JEXBIO_204_7_1247C31","doi-asserted-by":"publisher","first-page":"665","DOI":"10.1007\/BF00301135","article-title":"Modulation of catecholamine storage and release by the pituitary\u2013interrenal axis in the rainbow trout, Oncorhynchus mykiss","volume":"B165","author":"Reid","year":"1996","journal-title":"J. Comp. Physiol."},{"key":"2024121805234764200_JEXBIO_204_7_1247C32","doi-asserted-by":"publisher","first-page":"79","DOI":"10.1016\/0301-0082(84)90013-3","article-title":"The pharmacology of molluscan neurons","volume":"23","author":"Rosza","year":"1984","journal-title":"Prog. Neurobiol."},{"key":"2024121805234764200_JEXBIO_204_7_1247C33","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1016\/S0169-328X(98)00103-X","article-title":"Putative leech dopamine1-like receptor molecular characterization: sequence homologies between dopamine and serotonin leech CNS receptors explain pharmacological crossreactivities","volume":"58","author":"Salzet","year":"1998","journal-title":"Mol. Brain Res."},{"key":"2024121805234764200_JEXBIO_204_7_1247C34","doi-asserted-by":"crossref","first-page":"5400","DOI":"10.4049\/jimmunol.159.11.5400","article-title":"Leech immunocytes contain proopiomelanocortin: nitric oxide mediates hemolymph proopiomelanocortin processing","volume":"159","author":"Salzet","year":"1997","journal-title":"J. Immunol."},{"key":"2024121805234764200_JEXBIO_204_7_1247C35","doi-asserted-by":"publisher","first-page":"230","DOI":"10.1176\/jnp.10.2.230a","article-title":"A syndrome produced by diverse nocuous agents. 1936. (Classical Article)","volume":"10","author":"Selye","year":"1998","journal-title":"J. Neuropsych. Clin. Neurosci."},{"key":"2024121805234764200_JEXBIO_204_7_1247C36","doi-asserted-by":"publisher","first-page":"193","DOI":"10.1016\/S0021-9673(01)95373-7","article-title":"High-performance liquid chromatography of biogenic amines in the corpus cardiacum of the American cockroach, Periplaneta americana","volume":"539","author":"Shimizu","year":"1991","journal-title":"J. Chromatogr"},{"key":"2024121805234764200_JEXBIO_204_7_1247C37","doi-asserted-by":"publisher","first-page":"175","DOI":"10.1007\/BF00734572","article-title":"Highperformance liquid chromatographic analysis of monoamines and some of their \u03b3-glutamyl conjugates produced by the brain and other tissues of Helix aspersa (Gastropoda)","volume":"10","author":"Sloley","year":"1990","journal-title":"Cell. Mol. Neurobiol."},{"key":"2024121805234764200_JEXBIO_204_7_1247C38","doi-asserted-by":"publisher","first-page":"1254","DOI":"10.1139\/z98-063","article-title":"Distribution of catecholamines in the sea scallop, Placopecten magellanicus","volume":"76","author":"Smith","year":"1998","journal-title":"Can. J. Zool."},{"key":"2024121805234764200_JEXBIO_204_7_1247C39","doi-asserted-by":"publisher","first-page":"11099","DOI":"10.1073\/pnas.90.23.11099","article-title":"Opiate-like substances in an invertebrate, a novel opiate receptor on invertebrate and human immunocytes and a role in immunosuppression","volume":"90","author":"Stefano","year":"1993","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2024121805234764200_JEXBIO_204_7_1247C40","doi-asserted-by":"publisher","first-page":"9316","DOI":"10.1073\/pnas.89.19.9316","article-title":"(D-Ala2)-Deltorphin I binding and pharmacological evidence for a special subtype of delta opioid receptor on human and invertebrate immune cells","volume":"89","author":"Stefano","year":"1992","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2024121805234764200_JEXBIO_204_7_1247C41","doi-asserted-by":"publisher","first-page":"261","DOI":"10.1016\/S0074-7696(08)62420-1","article-title":"Invertebrate opioid precursors: evolutionary conservation and the significance of enzymatic processing","volume":"187","author":"Stefano","year":"1999","journal-title":"Int. Rev. Cytol."},{"key":"2024121805234764200_JEXBIO_204_7_1247C42","first-page":"340","article-title":"Mytilus edulis hemolymph contains pro-opiomelanocortin: LPS and morphine stimulate differential processing","volume":"63","author":"Stefano","year":"1999","journal-title":"Brain Res."},{"key":"2024121805234764200_JEXBIO_204_7_1247C43","first-page":"511","article-title":"Biogenic monoamine levels in the central nervous system of the sea hare, Aplysia kurodai","volume":"103C","author":"Takeda","year":"1992","journal-title":"Comp. Biochem. Physiol."},{"key":"2024121805234764200_JEXBIO_204_7_1247C44","doi-asserted-by":"publisher","first-page":"1178","DOI":"10.1177\/24.11.63507","article-title":"Ultrastructural cytochemistry of biogenic amines in nervous tissue: methodologic improvements","volume":"24","author":"Tranzer","year":"1976","journal-title":"J. Histochem. Cytochem."},{"key":"2024121805234764200_JEXBIO_204_7_1247C45","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1007\/978-1-4615-9804-6_4","article-title":"Amine transmitters and their associated second messenger systems","volume-title":"Comparative Invertebrate Neurochemistry","author":"Vaughan","year":"1988"},{"key":"2024121805234764200_JEXBIO_204_7_1247C46","doi-asserted-by":"publisher","first-page":"285","DOI":"10.1002\/(SICI)1096-9861(19990215)404:3&lt;285::AID-CNE1&gt;3.0.CO;2-S","article-title":"Development of catecholaminergic neurons in the pond snail, Lymnea stagnalis. I. Embryonic development of dopaminecontaining neurons and dopamine-dependent behaviors","volume":"404","author":"Voronezhskaya","year":"1999","journal-title":"J. Comp. Neurol."},{"key":"2024121805234764200_JEXBIO_204_7_1247C47","doi-asserted-by":"publisher","first-page":"591","DOI":"10.1152\/physrev.1997.77.3.591","article-title":"The stress response in fish","volume":"77","author":"Wendelaar Bonga","year":"1997","journal-title":"Physiol. Rev."}],"container-title":["Journal of Experimental Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.biologists.com\/jeb\/article-pdf\/204\/7\/1247\/3605978\/jexbio_204_7_1247.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.biologists.com\/jeb\/article-pdf\/204\/7\/1247\/3605978\/jexbio_204_7_1247.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,18]],"date-time":"2024-12-18T00:24:02Z","timestamp":1734481442000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.biologists.com\/jeb\/article\/204\/7\/1247\/8737\/Evidence-for-a-form-of-adrenergic-response-to"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,4,1]]},"references-count":47,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2001,4,1]]}},"URL":"https:\/\/doi.org\/10.1242\/jeb.204.7.1247","relation":{},"ISSN":["0022-0949","1477-9145"],"issn-type":[{"value":"0022-0949","type":"print"},{"value":"1477-9145","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,4,1]]}}}