{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T06:14:41Z","timestamp":1791353681588,"version":"4.3.1"},"reference-count":41,"publisher":"The Company of Biologists","license":[{"start":{"date-parts":[[2019,1,1]],"date-time":"2019-01-01T00:00:00Z","timestamp":1546300800000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"crossref","award":["PDF-502414-2017"],"award-info":[{"award-number":["PDF-502414-2017"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>In water-breathing fishes, the hypoxic ventilatory response (HVR) represents an increase in water flow over the gills during exposure to lowered ambient O2 levels. The HVR is a critical defense mechanism that serves to delay the negative consequences of hypoxia on aerobic respiration. However, the physiological significance of the HVR in larval fishes is unclear as they do not have a fully developed gill and rely primarily on cutaneous gas transfer. Using larval zebrafish (4, 7, 10, and 15 days post fertilization; dpf), we examined their HVR under three levels of hypoxia (25, 45 and 60 mmHg). The larvae exhibited widely different HVRs as a function of developmental age and level of the hypoxia. Yet, critical O2 tensions (Pcrit) remained constant (30 - 34 mmHg) over the same period of development. Micro-optrode O2 sensors were used to measure a significant decrease in buccal cavity water O2 tensions in 4 and 7 dpf larvae compared to the water they inspired, demonstrating significant extraction of O2 from the buccal cavity. To assess the physiological significance of the HVR, ventilatory water flow was prevented in larvae at 4 and 7 dpf by embedding their heads in agar. An increase in Pcrit was observed in 7 but not 4 dpf larvae, suggesting that buccal ventilation is important for O2 extraction by 7 dpf. Combined, these data indicate that branchial\/buccal gas transfer plays a significant role in O2 uptake during hypoxia, and supports a physiological benefit of the HVR in early life stages of zebrafish.<\/jats:p>","DOI":"10.1242\/jeb.204800","type":"journal-article","created":{"date-parts":[[2019,6,13]],"date-time":"2019-06-13T15:05:15Z","timestamp":1560438315000},"source":"Crossref","is-referenced-by-count":25,"title":["Evaluating the physiological significance of hypoxic hyperventilation in larval zebrafish (<i>Danio rerio<\/i>)"],"prefix":"10.1242","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1455-1439","authenticated-orcid":false,"given":"Yihang K.","family":"Pan","sequence":"first","affiliation":[{"name":"Department of Biology, University of Ottawa, 30 Marie Curie, Ottawa, Ontario K1N 6N5 Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Milica","family":"Mandic","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Ottawa, 30 Marie Curie, Ottawa, Ontario K1N 6N5 Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alex M.","family":"Zimmer","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Ottawa, 30 Marie Curie, Ottawa, Ontario K1N 6N5 Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steve F.","family":"Perry","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Ottawa, 30 Marie Curie, Ottawa, Ontario K1N 6N5 Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2019,1,1]]},"reference":[{"key":"2021042500342160100_JEB204800C1","first-page":"403","article-title":"Physicochemical parameters for use in fish respiratory physiology","volume-title":"Fish Physiology","author":"Boutilier","year":"1984"},{"key":"2021042500342160100_JEB204800C2","doi-asserted-by":"publisher","first-page":"R689","DOI":"10.1152\/ajpregu.00022.2016","article-title":"Developmental cardiorespiratory physiology of the air-breathing tropical gar, Atractosteus tropicus","volume":"311","author":"Burggren","year":"2016","journal-title":"Am. 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