{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T17:20:28Z","timestamp":1773422428858,"version":"3.50.1"},"reference-count":45,"publisher":"The Company of Biologists","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2002,6,1]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>Intracellular ion activities (aion) and basolateral membrane potential (Vbl) were measured in Malpighian tubule cells of Rhodnius prolixus using double-barrelled ion-selective microelectrodes. In saline containing 103mmoll-1Na+, 6mmoll-1 K+ and 93mmoll-1Cl-, intracellular ion activities in unstimulated upper Malpighian tubules were 21, 86 and 32mmoll-1, respectively. In serotonin-stimulated tubules, aCl was unchanged, whereas aNa increased to 33mmoll-1 and aK declined to 71mmoll-1. Vbl was -59mV and -63mV for unstimulated and stimulated tubules, respectively. Calculated electrochemical potentials(\u0394\u03bc\/F) favour passive movement of Na+ into the cell and passive movement of Cl- out of the cell in both unstimulated and serotonin-stimulated tubules. Passive movement of K+ out of the cell is favoured in unstimulated tubules. In stimulated tubules, \u0394\u03bc\/F for K+ is close to 0 mV.<\/jats:p><jats:p>The thermodynamic feasibilities of Na+-K+-2Cl-, Na+-Cl-and K+-Cl- cotransporters were evaluated by calculating the net electrochemical potential (\u0394\u03bcnet\/F) for each transporter. Our results show that a Na+-K+-2Cl- or a Na+-Cl- cotransporter but not a K+-Cl- cotransporter would permit the movement of ions into the cell in stimulated tubules. The effects of Ba2+ and ouabain on Vbl and rates of fluid and ion secretion show that net entry of K+ through ion channels or the Na+\/K+-ATPase can be ruled out in stimulated tubules. Maintenance of intracellular Cl- activity was dependent upon the presence of both Na+ and K+ in the bathing saline. Bumetanide reduced the fluxes of both Na+ and K+. Taken together, the results support the involvement of a basolateral Na+-K+-2Cl- cotransporter in serotonin-stimulated fluid secretion by Rhodnius prolixus Malpighian tubules.<\/jats:p>","DOI":"10.1242\/jeb.205.11.1645","type":"journal-article","created":{"date-parts":[[2021,4,25]],"date-time":"2021-04-25T04:58:15Z","timestamp":1619326695000},"page":"1645-1655","source":"Crossref","is-referenced-by-count":42,"title":["Intracellular ion activities in Malpighian tubule cells of<i>Rhodnius prolixus<\/i>: evaluation of Na+-K+-2Cl-cotransport across the basolateral membrane"],"prefix":"10.1242","volume":"205","author":[{"given":"Juan P.","family":"Ianowski","sequence":"first","affiliation":[{"name":"Department of Biology, McMaster University, 1280 Main Street West,Hamilton, Ontario, Canada L8S 4K1"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert J.","family":"Christensen","sequence":"additional","affiliation":[{"name":"Department of Biology, McMaster University, 1280 Main Street West,Hamilton, Ontario, Canada L8S 4K1"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael J.","family":"O'Donnell","sequence":"additional","affiliation":[{"name":"Department of Biology, McMaster University, 1280 Main Street West,Hamilton, Ontario, Canada L8S 4K1"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","reference":[{"key":"2021042504545409500_REF1","doi-asserted-by":"crossref","unstructured":"Al-Fifi, Z. 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