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In contrast to samples from other organs, the protein analysis of mouse testis identified predominantly full-length 23 kDa Alrp. This finding prompted us to investigate in more detail the expression of Alrp during spermatogenesis. Testis samples of individual mice from postnatal days 13-29 were probed with an antibody specific for mammalian Alrp. In addition, cells from whole testis preparations were fractionated on a bovine serum albumin column gradient. Protein expression of mouse Alrp was compared with those of testis-specific cyritestin, the cytoskeleton marker actin and mitochondrial subunit Vb of cytochrome oxidase and cytochrome c. These studies demonstrated a specific accumulation of full-length mouse Alrp during the early stages of spermatogenesis. The highest levels of Alrp were found in spermatogonia and primary spermatocytes. Levels of expression of Alrp did not correlate with the synthesis of components of the respiratory chain,indicating that full-length Alrp in the mitochondria of spermatogonia and spermatocytes has another function in addition to its role in oxidative phosphorylation.<\/jats:p>","DOI":"10.1242\/jeb.205.14.1979","type":"journal-article","created":{"date-parts":[[2021,4,25]],"date-time":"2021-04-25T04:59:36Z","timestamp":1619326776000},"page":"1979-1986","source":"Crossref","is-referenced-by-count":33,"title":["Accumulation of the mitochondrial form of the sulphydryl oxidase Erv1p\/Alrp during the early stages of spermatogenesis"],"prefix":"10.1242","volume":"205","author":[{"given":"Monika","family":"Klissenbauer","sequence":"first","affiliation":[{"name":"Institut fu\u0308r Botanik, Heinrich-Heine-Universita\u0308t Du\u0308sseldorf, Universita\u0308tsstra\u00dfe, D-40225 Du\u0308sseldorf,Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silke","family":"Winters","sequence":"additional","affiliation":[{"name":"Institut fu\u0308r Genetik, Heinrich-Heine-Universita\u0308t Du\u0308sseldorf, Universita\u0308tsstra\u00dfe, D-40225 Du\u0308sseldorf,Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Uwe A. O.","family":"Heinlein","sequence":"additional","affiliation":[{"name":"Institut fu\u0308r Genetik, Heinrich-Heine-Universita\u0308t Du\u0308sseldorf, Universita\u0308tsstra\u00dfe, D-40225 Du\u0308sseldorf,Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Lisowsky","sequence":"additional","affiliation":[{"name":"Institut fu\u0308r Botanik, Heinrich-Heine-Universita\u0308t Du\u0308sseldorf, Universita\u0308tsstra\u00dfe, D-40225 Du\u0308sseldorf,Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","reference":[{"key":"2021042504555193300_REF1","doi-asserted-by":"crossref","unstructured":"Becher, D., Kricke, J., Stein, G. and Lisowsky, T.(1999). A mutant for the yeast scERV1 gene displays a new defect in mitochondrial morphology and distribution. 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Stimulation of the mitogen-activated protein kinase cascade and tyrosine phosphorylation of the epidermal growth factor receptor by hepatopoietin. J. Biol. Chem. 275,37443-37447.","DOI":"10.1074\/jbc.M004373200"},{"key":"2021042504555193300_REF30","doi-asserted-by":"crossref","unstructured":"Lill, R., Diekert, K., Kaut, A., Lange, H., Pelzer, W., Prohl,C. and Kispal, G. (1999). The essential role of mitochondria in the biogenesis of cellular iron\u2014sulphur proteins. Biol. Chem. 380,1157-1166.","DOI":"10.1515\/BC.1999.147"},{"key":"2021042504555193300_REF31","doi-asserted-by":"crossref","unstructured":"Lill, R. and Kispal, G. (2000). Maturation of cellular Fe\u2014S proteins: an essential function of mitochondria. Trends Biochem. Sci.25,352-356.","DOI":"10.1016\/S0968-0004(00)01589-9"},{"key":"2021042504555193300_REF32","doi-asserted-by":"crossref","unstructured":"Linder, B., Bammer, S. and Heinlein, U. A. O.(1995). 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