{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T23:25:31Z","timestamp":1784589931478,"version":"3.55.0"},"reference-count":57,"publisher":"The Company of Biologists","issue":"17","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2002,9,1]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>A cDNA encoding the homologue of mammalian aquaporin 3 (AQP-3) was isolated by reverse transcription\u2014polymerase chain reaction from the gill of the European eel. The derived amino acid sequence shares 67-70% homology with other vertebrate AQP-3 homologues. Northern blot analysis revealed two AQP-3-specific mRNA species of 2.4 kb and 7 kb. AQP-3 mRNA is expressed predominantly in the eye, oesophagus, intestine (as found in mammals) and the gill; no expression could be demonstrated in the stomach and only low and sporadic levels in the kidney. Quantitative studies demonstrated that,following the 3-week acclimation of freshwater (FW)-adapted yellow and silver eels to seawater (SW), transcript abundance in the gill was reduced by 76% and 97%, respectively. The half time of branchial AQP-3 mRNA downregulation in yellow eels was approximately 10 h, with a maximal 94% decrease in expression after 2 days in SW (compared to time-matched FW controls). However, in fish acclimated to SW for more than 4 days, the fall in AQP-3 mRNA abundance recovered slightly, such that after 3 weeks, expression was 16% of that in time-matched FW controls. The potential roles for this aquaporin isoform in water or solute transport in the eel gill are discussed.<\/jats:p>","DOI":"10.1242\/jeb.205.17.2643","type":"journal-article","created":{"date-parts":[[2021,4,25]],"date-time":"2021-04-25T05:01:47Z","timestamp":1619326907000},"page":"2643-2651","source":"Crossref","is-referenced-by-count":118,"title":["Branchial expression of an aquaporin 3 (AQP-3) homologue is downregulated in the European eel<i>Anguilla anguilla<\/i>following seawater acclimation"],"prefix":"10.1242","volume":"205","author":[{"given":"Christopher P.","family":"Cutler","sequence":"first","affiliation":[{"name":"School of Biology, Bute Medical Buildings, University of St Andrews,St Andrews, Fife KY16 9TS, Scotland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gordon","family":"Cramb","sequence":"additional","affiliation":[{"name":"School of Biology, Bute Medical Buildings, University of St Andrews,St Andrews, Fife KY16 9TS, Scotland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","reference":[{"key":"2021042504574508200_REF1","doi-asserted-by":"crossref","unstructured":"Bergman, H. L., Olson, K. R. and Fromm, P. O.(1974). The effects of vasoactive agents on the functional surface area of isolated-perfused gills of rainbow trout. J. Comp. Physiol.94,267-286.","DOI":"10.1007\/BF00710640"},{"key":"2021042504574508200_REF2","unstructured":"Bill, R. M., Hedfalk, K., Kalgren, S., Rydstrom, J. and Hohmann,S. (2000). Functional analysis of the unusual signature motifs of the yeast mip channel Fps 1p. In Molecular Biology and Physiology of Water and Solute Transport (ed. S. Hohmann and S. Neilsen), pp. 3-11. New York, London: Kluwer Academic\/Plenum Publishers."},{"key":"2021042504574508200_REF3","doi-asserted-by":"crossref","unstructured":"Birrell, L., Cramb, G. and Hazon, N. (2000). Osmoregulation during the development of glass eels and elvers. J. Fish Biol.56,1450-1459.","DOI":"10.1111\/j.1095-8649.2000.tb02156.x"},{"key":"2021042504574508200_REF4","doi-asserted-by":"crossref","unstructured":"Borgnia, M., Nielsen, S., Engel, A. and Agre, P.(1999). 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L., Davis, B. A., Romero, M. F. and Boron, W. F.(1998). Effect of expressing the water channel aquaporin-1 on the CO2 permeability of Xenopus oocytes. Am. J. Physiol.274,C543-C548."},{"key":"2021042504574508200_REF37","unstructured":"Nakhoul, N. L., Hering-Smith, K. S., Abdulnour-Nakhoul, S. M. and Hamm, L. L. (2001). Transport of NH3\/NH4+ in oocytes expressing aquaporin-1. Am. J. Physiol.281,F255-F263"},{"key":"2021042504574508200_REF38","doi-asserted-by":"crossref","unstructured":"Ogasawara, T. and Hirano, T. (1984). Effects of prolactin and environmental calcium on osmotic water permeability of the gills in the eel Anguilla japonica.Gen. Comp. Endocrinol.53,315-324.","DOI":"10.1016\/0016-6480(84)90258-2"},{"key":"2021042504574508200_REF39","unstructured":"Ogawa, M. (1974). The effects of bovine prolactin, sea water and environmental calcium on water influx in isolated gills of the euryhaline teleosts, Anguilla japonica and Salmo gairdnerii.Comp. Biochem. 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