{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T03:04:01Z","timestamp":1789873441541,"version":"4.0.1"},"reference-count":58,"publisher":"The Company of Biologists","issue":"19","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2002,10,1]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>Many marine dinoflagellates emit bright discrete flashes of light nearly instantaneously in response to either laminar or turbulent flows as well as to direct mechanical stimulation. The flash involves a unique pH-dependent luciferase and a proton-mediated action potential across the vacuole membrane. The mechanotransduction process initiating this action potential is unknown. The present study investigated the role of Ca2+ in the mechanotransduction process regulating bioluminescence in the dinoflagellate Lingulodinium polyedrum. Calcium ionophores and digitonin stimulated luminescence in a Ca2+-dependent manner in the absence of mechanical stimulation. Mechanically sensitive luminescence was strongly inhibited by the intracellular Ca2+ chelator BAPTA-AM[1,2-bis(o-aminophenoxy)ethane-N,N,N\u2032,N\u2032-tetraacetic acid acetoxymethyl ester]; there was only a partial and irreversible dependence on extracellular Ca2+. Ruthenium Red, a blocker of intracellular Ca2+ release channels, inhibited mechanically sensitive luminescence. Luminescence was also stimulated by increasing K+, even in the absence of extracellular Ca2+; K+ stimulation was inhibited both by BAPTA-AM and Ruthenium Red. These results support the hypothesis that Ca2+mediates stimulated bioluminescence and also indicate the involvement of intracellular Ca2+ stores. Rapid coupling between mechanical stimulation and mobilization of intracellular Ca2+ stores might occur through a mechanism similar to excitation-contraction coupling in skeletal muscle.<\/jats:p>","DOI":"10.1242\/jeb.205.19.2971","type":"journal-article","created":{"date-parts":[[2021,4,25]],"date-time":"2021-04-25T04:58:47Z","timestamp":1619326727000},"page":"2971-2986","source":"Crossref","is-referenced-by-count":57,"title":["The role of Ca2+ in stimulated bioluminescence of the dinoflagellate<i>Lingulodinium polyedrum<\/i>"],"prefix":"10.1242","volume":"205","author":[{"given":"Peter","family":"von Dassow","sequence":"first","affiliation":[{"name":"Scripps Institution of Oceanography, University of California, San Diego, La Jolla, CA 92037-0202, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael I.","family":"Latz","sequence":"additional","affiliation":[{"name":"Scripps Institution of Oceanography, University of California, San Diego, La Jolla, CA 92037-0202, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","reference":[{"key":"2021042504551465400_REF1","doi-asserted-by":"crossref","unstructured":"Blaustein, M. 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