{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T21:32:24Z","timestamp":1791235944813,"version":"4.1.0"},"reference-count":45,"publisher":"The Company of Biologists","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2002,3,15]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>Sections of posterior intestine of the euryhaline killifish Fundulus heteroclitus adapted to sea water were stimulated by the calcium ionophore ionomycin (1\u00a0\u03bcmol\u00a0l\u20131) in combination with agents to elevate intracellular cyclic AMP levels, 0.5\u00a0mmol\u00a0l\u20131 dibutyryl-cyclic AMP (db-cAMP) with 0.1\u00a0mmol\u00a0l\u20131 3-isobutyl-1-methylxanthine (IBMX). Intestinal bag preparations from recently fed animals (but not from overnight unfed animals) changed from fluid absorption (+18.9\u00b18.30\u00a0\u03bcl\u00a0cm\u20132\u00a0h\u20131 , N=8) in the untreated control period to net fluid secretion after stimulation (\u20137.43\u00b11.30\u00a0\u03bcl\u00a0cm\u20132\u00a0h\u20131, N=8, P&amp;lt;0.01; means \u00b1 s.e.m.), indicative of the capacity of teleost intestine to undergo secretion. Posterior intestinal pieces mounted in vitro in Ussing-style membrane chambers showed net Cl\u2013 uptake (+2.245\u00b10.633\u00a0\u03bcequiv\u00a0cm\u20132\u00a0h\u20131, N=7) that turned to net secretion following stimulation by ionomycin + db-cAMP + IBMX (\u20133.809\u00b11.22\u00a0\u03bcequiv\u00a0cm\u20132\u00a0h\u20131, N=7, P&amp;lt;0.01). Mucosal application of the anion channel blocker 1\u00a0mmol\u00a0l\u20131 diphenylamine-2-carboxylate (DPC) after ionomycin + db-cAMP + IBMX treatment significantly reduced serosal-to-mucosal unidirectional Cl\u2013 flux (P&amp;lt;0.001), net Cl\u2013 flux (P&amp;lt;0.05), short-circuit current (Isc, P&amp;lt;0.001) and tissue conductance (Gt, P&amp;lt;0.001), while 0.1\u00a0mmol\u00a0l\u20131 4,4\u2032-diisothiocyano-2,2\u2032-stilbene-disulphonic acid (DIDS, a blocker of anion exchange) was without effect. Stimulation by db-cAMP + IBMX (no ionomycin) significantly increased unidirectional fluxes, Isc and Gt but did not produce net Cl\u2013 secretion. Ionomycin alone produced a transient increase in Isc but had no effect on Gt and caused no significant changes in unidirectional or net Cl\u2013 fluxes. Addition of db-cAMP + IBMX after ionomycin treatment produced net secretion of Cl\u2013 and large increases in unidirectional fluxes and Gt. Cystic fibrosis transmembrane conductance regulator (CFTR) was immunocytochemically localized with a monoclonal mouse antibody to the carboxy terminus and found to be present in the cytoplasm and basolateral membranes of all enterocytes and in the brush-border membrane of some cells, whereas NKCC immunofluorescence, demonstrating the presence of the Na+\/K+\/2Cl\u2013 cotransporter, was present in the cytoplasm and brush-border membrane. We conclude that the teleost intestine is capable of salt and fluid secretion only if intracellular Ca2+ and cyclic AMP pathways are stimulated together and that this secretion appears to involve activation of CFTR ion channels in the apical membrane of a subpopulation of enterocytes.<\/jats:p>","DOI":"10.1242\/jeb.205.6.745","type":"journal-article","created":{"date-parts":[[2021,4,25]],"date-time":"2021-04-25T04:52:39Z","timestamp":1619326359000},"page":"745-758","source":"Crossref","is-referenced-by-count":82,"title":["NaCl and fluid secretion by the intestine of the teleost<i>Fundulus heteroclitus<\/i>: involvement of CFTR"],"prefix":"10.1242","volume":"205","author":[{"given":"W. S.","family":"Marshall","sequence":"first","affiliation":[{"name":"Department of Biology, St Francis Xavier University, Antigonish, Nova Scotia, Canada B2G 2W5"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. 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