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As physical performance often affects the outcome of predator-prey interactions, environmental temperature can influence the ability of ectotherms to capture prey and\/or defend themselves against predators. However, previous research on the kinematics of ectotherms suggests that some species may use elastic storage mechanisms when attacking or defending, thereby mitigating the effects of sub-optimal temperature. Rattlesnakes (Crotalus) are a speciose group of ectothermic viperid snakes that rely on crypsis, rattling, and striking to deter predators. We examined the influence of body temperature on the behavior and kinematics of two rattlesnake species (C. oreganus helleri and C. scutulatus) when defensively striking towards a threatening stimulus. We recorded defensive strikes at body temperatures ranging from 15\u00b0C\u201335\u00b0C. We found that strike speed and speed of mouth gaping during the strike were positively correlated with temperature. We also found a marginal effect of temperature on the probability of striking, latency to strike, and strike outcome. Overall, warmer snakes are more likely to strike, strike faster, open their mouth faster, and reach maximum gape earlier than colder snakes. However, the effects of temperature were less than would be expected for purely muscle-driven movements. Our results suggest that, although rattlesnakes are at a greater risk of predation at colder body temperatures, their decrease in strike performance may be mitigated to some extent by employing mechanisms in addition to skeletal muscle contraction (e.g. elastic energy storage) to power strikes.<\/jats:p>","DOI":"10.1242\/jeb.223859","type":"journal-article","created":{"date-parts":[[2020,6,19]],"date-time":"2020-06-19T16:49:33Z","timestamp":1592585373000},"source":"Crossref","is-referenced-by-count":21,"title":["The effects of temperature on the defensive strikes of rattlesnakes"],"prefix":"10.1242","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2516-2607","authenticated-orcid":false,"given":"Malachi D.","family":"Whitford","sequence":"first","affiliation":[{"name":"Department of Biology, San Diego State University, San Diego, CA, USA"},{"name":"Ecology Graduate Group, University of California, Davis, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7786-0610","authenticated-orcid":false,"given":"Grace A.","family":"Freymiller","sequence":"additional","affiliation":[{"name":"Department of Biology, San Diego State University, San Diego, CA, USA"},{"name":"Department of Evolution, Ecology, and Organismal Biology, University of California, Riverside, CA 92521, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3538-6671","authenticated-orcid":false,"given":"Timothy E.","family":"Higham","sequence":"additional","affiliation":[{"name":"Department of Evolution, Ecology, and Organismal Biology, University of California, Riverside, CA 92521, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1100-1856","authenticated-orcid":false,"given":"Rulon W.","family":"Clark","sequence":"additional","affiliation":[{"name":"Department of Biology, San Diego State University, San Diego, CA, USA"},{"name":"Chiricahua Desert Museum, Rodeo, NM, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2020,1,1]]},"reference":[{"key":"2021042623545217200_JEB223859C1","doi-asserted-by":"publisher","first-page":"5495","DOI":"10.1073\/pnas.0910778107","article-title":"Ballistic tongue projection in chameleons maintains high performance at low temperature","volume":"107","author":"Anderson","year":"2010","journal-title":"Proc. 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