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By suppressing action potentials in excitatory glutamatergic neurons, the goldfish brain decreases its overall energy expenditure. Coincident with reductions in O2 availability is a natural decrease in cellular reactive oxygen species (ROS) generation, which has been proposed to function as part of a low-oxygen signal transduction pathway. Using live-tissue fluorescence microscopy, we found that ROS production decreased by 10% with the onset of anoxia in goldfish telencephalic brain slices. Employing whole-cell patch-clamp recording, we found that, similar to severe hypoxia, the ROS scavengers N-acetyl cysteine (NAC) and MitoTEMPO, added during normoxic periods, depolarized membrane potential (severe hypoxia \u221273.6 to \u221261.4\u2005mV, NAC \u221276.6 to \u221266.2\u2005mV and MitoTEMPO \u221271.5\u2005mV to \u221262.5\u2005mV) and increased whole-cell conductance (severe hypoxia 5.7\u00a0nS to 8.0\u2005nS, NAC 6.0\u2005nS to 7.5\u2005nS and MitoTEMPO 6.0\u2005nS to 7.6\u2005nS). Also, in a subset of active pyramidal neurons, these treatments reduced action potential firing frequency (severe hypoxia 0.18\u2005Hz to 0.03\u2005Hz, NAC 0.27\u2005Hz to 0.06\u2005Hz and MitoTEMPO 0.35\u2005Hz to 0.08\u2005Hz). Neither severe hypoxia nor ROS scavenging impacted action potential threshold. The addition of exogenous hydrogen peroxide could reverse the effects of the antioxidants. Taken together, this supports a role for a reduction in [ROS] as a low-oxygen signal in goldfish brain.<\/jats:p>","DOI":"10.1242\/jeb.238147","type":"journal-article","created":{"date-parts":[[2021,4,30]],"date-time":"2021-04-30T10:05:15Z","timestamp":1619777115000},"source":"Crossref","is-referenced-by-count":7,"title":["Scavenging of reactive oxygen species mimics the anoxic response in goldfish pyramidal neurons"],"prefix":"10.1242","volume":"224","author":[{"given":"Varshinie","family":"Pillai","sequence":"first","affiliation":[{"name":"Department of Cell & Systems Biology, University of Toronto, Toronto, ON, Canada, M3A 3A7"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2637-7821","authenticated-orcid":false,"given":"Leslie","family":"Buck","sequence":"additional","affiliation":[{"name":"Department of Cell & Systems Biology, University of Toronto, Toronto, ON, Canada, M3A 3A7"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ebrahim","family":"Lari","sequence":"additional","affiliation":[{"name":"Department of Cell & Systems Biology, University of Toronto, Toronto, ON, Canada, M3A 3A7"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2021,5,28]]},"reference":[{"key":"2021060110333690900_JEB238147C1","doi-asserted-by":"publisher","first-page":"413","DOI":"10.1016\/0896-6273(90)90053-I","article-title":"Excitotoxicity induced by enhanced excitatory neurotransmission in cultured hippocampal pyramidal neurons","volume":"4","author":"Abele","year":"1990","journal-title":"Neuron"},{"key":"2021060110333690900_JEB238147C2","doi-asserted-by":"publisher","first-page":"S413","DOI":"10.3233\/JAD-2010-100465","article-title":"Calcium and mitochondrial reactive oxygen species generation: How to read the facts","volume":"20","author":"Adam-Vizi","year":"2010","journal-title":"J. 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