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On each fiber bundle, we performed active and passive stretches from an average sarcomere length of 2.6\u20133.0\u2005\u00b5m at a slow rate of 0.04\u2005\u00b5m\u00a0s\u22121, as well as isometric contractions at the initial and final lengths. One-way ANOVA showed that SOL and EDL fiber bundles from mdm mice exhibited significantly lower RFE than WT mice (P&amp;lt;0.0001). This result is consistent with previous observations in single myofibrils and intact muscles. However, it contradicts the results from a previous study that appeared to show that compensatory mechanisms could restore titin force enhancement in single fibers from mdm psoas. We suggest that RFE measured previously in mdm single fibers was an artifact of the high variability in passive tension found in degenerating fibers, which begins after \u223c24\u2005days of age. The results are consistent with the hypothesis that RFE is reduced in mdm skeletal muscles owing to impaired Ca2+-dependent titin\u2013actin interactions resulting from the small deletion in N2A titin.<\/jats:p>","DOI":"10.1242\/jeb.243732","type":"journal-article","created":{"date-parts":[[2022,5,6]],"date-time":"2022-05-06T08:22:28Z","timestamp":1651825348000},"source":"Crossref","is-referenced-by-count":8,"title":["Residual force enhancement is reduced in permeabilized fiber bundles from <i>mdm<\/i> muscles"],"prefix":"10.1242","volume":"225","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3652-6189","authenticated-orcid":false,"given":"Dhruv","family":"Mishra","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, Northern Arizona University, Flagstaff, AZ 86011, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8252-0285","authenticated-orcid":false,"given":"Kiisa C.","family":"Nishikawa","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Northern Arizona University, Flagstaff, AZ 86011, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2022,5,26]]},"reference":[{"key":"2022052608110406700_JEB243732C1","doi-asserted-by":"publisher","first-page":"77","DOI":"10.1113\/jphysiol.1952.sp004755","article-title":"The force exerted by active striated muscle during and after change of length","volume":"117","author":"Abbott","year":"1952","journal-title":"J. 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