{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,18]],"date-time":"2025-12-18T19:59:42Z","timestamp":1766087982888,"version":"3.41.2"},"reference-count":44,"publisher":"The Company of Biologists","issue":"2","license":[{"start":{"date-parts":[[2023,1,30]],"date-time":"2023-01-30T00:00:00Z","timestamp":1675036800000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100006087","name":"Direcci\u00f3n General de Asuntos del Personal Acad\u00e9mico, Universidad Nacional Aut\u00f3noma de M\u00e9xico","doi-asserted-by":"crossref","award":["IN218123","CD.CBS.481.2021","IN207122"],"award-info":[{"award-number":["IN218123","CD.CBS.481.2021","IN207122"]}],"id":[{"id":"10.13039\/501100006087","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Programa para el Desarrollo Profesional Docente","award":["UAM-PTC-675"],"award-info":[{"award-number":["UAM-PTC-675"]}]},{"DOI":"10.13039\/501100003141","name":"Consejo Nacional de Ciencia y Tecnolog\u00eda","doi-asserted-by":"crossref","award":["925549"],"award-info":[{"award-number":["925549"]}],"id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100005739","name":"Universidad Nacional Aut\u00f3noma de M\u00e9xico","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100005739","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,1,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Mammalian sperm capacitation involves biochemical and physiological changes, such as an increase in intracellular calcium ion concentration ([Ca2+]i), hyperpolarization of the plasma membrane potential and sperm hyperactivation, among others. These changes provide sperm with the ability to fertilize. In the bat Corynorhinus mexicanus, there is an asynchrony between spermatogenesis and sperm storage in the male with the receptivity of the female. For instance, in C. mexicanus, spermatogenesis occurs before the reproductive season. During the reproductive period, sperm are stored in the epididymis for a few months and the testis undergoes a regression, indicating low or almost null sperm production. Therefore, it is unclear whether the elements necessary for sperm fertilization success undergo maturation or preparation during epididymis storage. Here, we characterized pH-sensitive motility hyperactivation and Ca2+ influx in sperm, regulated by alkalinization and progesterone. In addition, by electrophysiological recordings, we registered currents that were stimulated by alkalinization and inhibited by RU1968 (a CatSper-specific inhibitor), strongly suggesting that these currents were evoked via CatSper, a sperm Ca2+-specific channel indispensable for mammalian fertilization. We also found hyperpolarization of the membrane potential, such as in other mammalian species, which increased according to the month of capture, reaching the biggest hyperpolarization during the mating season. In conclusion, our results suggest that C. mexicanus sperm have functional CatSper and undergo a capacitation-like process such as in other mammals, particularly Ca2+ influx and membrane potential hyperpolarization.<\/jats:p>","DOI":"10.1242\/jeb.244878","type":"journal-article","created":{"date-parts":[[2022,12,21]],"date-time":"2022-12-21T10:08:50Z","timestamp":1671617330000},"source":"Crossref","is-referenced-by-count":3,"title":["Sperm calcium flux and membrane potential hyperpolarization observed in the Mexican big-eared bat <i>Corynorhinus mexicanus<\/i>"],"prefix":"10.1242","volume":"226","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3194-1175","authenticated-orcid":false,"given":"Jos\u00e9 Edwin","family":"Mendoza-S\u00e1nchez","sequence":"first","affiliation":[{"name":"Doctorado en Biolog\u00eda Experimental, Universidad Aut\u00f3noma Metropolitana 1 , Iztapalapa, 09310 Ciudad de M\u00e9xico , M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ahiezer","family":"Rodr\u00edguez-Tob\u00f3n","sequence":"additional","affiliation":[{"name":"Laboratorio de Biolog\u00eda y Ecolog\u00eda de Mam\u00edferos, Universidad Aut\u00f3noma Metropolitana 2 , Iztapalapa, 09310 Ciudad de M\u00e9xico , M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2534-9351","authenticated-orcid":false,"given":"Edith","family":"Arenas-R\u00edos","sequence":"additional","affiliation":[{"name":"Laboratorio de Morfofisiolog\u00eda y Bioqu\u00edmica del Espermatozoide, Universidad Aut\u00f3noma Metropolitana 3 , Iztapalapa, 09310 Ciudad de M\u00e9xico , M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6817-6673","authenticated-orcid":false,"given":"Gerardo J.","family":"Orta-Salazar","sequence":"additional","affiliation":[{"name":"Consorcio de Fisiolog\u00eda del Espermatozoide, Instituto de Biotecnolog\u00eda, Universidad Nacional Aut\u00f3noma de M\u00e9xico 4 , Cuernavaca, 62210 Morelos , M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miguel A.","family":"Le\u00f3n-Galv\u00e1n","sequence":"additional","affiliation":[{"name":"Laboratorio de Biolog\u00eda y Ecolog\u00eda de Mam\u00edferos, Universidad Aut\u00f3noma Metropolitana 2 , Iztapalapa, 09310 Ciudad de M\u00e9xico , M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia L.","family":"Trevi\u00f1o Santa Cruz","sequence":"additional","affiliation":[{"name":"Consorcio de Fisiolog\u00eda del Espermatozoide, Instituto de Biotecnolog\u00eda, Universidad Nacional Aut\u00f3noma de M\u00e9xico 4 , Cuernavaca, 62210 Morelos , M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7375-9531","authenticated-orcid":false,"given":"Julio C.","family":"Ch\u00e1vez","sequence":"additional","affiliation":[{"name":"Consorcio de Fisiolog\u00eda del Espermatozoide, Instituto de Biotecnolog\u00eda, Universidad Nacional Aut\u00f3noma de M\u00e9xico 4 , Cuernavaca, 62210 Morelos , M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[2023,1,30]]},"reference":[{"key":"2023013010173448100_JEB244878C1","doi-asserted-by":"publisher","first-page":"1802","DOI":"10.1093\/humrep\/dey280","article-title":"Effects of pH manipulation, CatSper stimulation and Ca2+-store mobilization on [Ca2+]i and behaviour of human sperm","volume":"33","author":"Achikanu","year":"2018","journal-title":"Hum. 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Pharmacol."},{"volume-title":"Estudio de la maduraci\u00f3n de espermatozoides epididimarios del murci\u00e9lago estacional Corynorhinus mexicanus","year":"2016","author":"Rodr\u00edguez-Tob\u00f3n","key":"2023013010173448100_JEB244878C27"},{"key":"2023013010173448100_JEB244878C28","doi-asserted-by":"publisher","first-page":"310","DOI":"10.1111\/azo.12124","article-title":"Tyrosine phosphorylation as evidence of epididymal cauda participation in the sperm maturation process of C orynorhinus mexicanus bat","volume":"97","author":"Rodr\u00edguez-Tob\u00f3n","year":"2016","journal-title":"Acta Zool."},{"key":"2023013010173448100_JEB244878C29","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1095\/biolreprod.115.136085","article-title":"A specific transitory increase in intracellular calcium induced by progesterone promotes acrosomal exocytosis in mouse sperm","volume":"94","author":"Romarowski","year":"2016","journal-title":"Biol. 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