{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T22:40:56Z","timestamp":1787784056623,"version":"build-2784847793"},"reference-count":54,"publisher":"The Company of Biologists","issue":"3","license":[{"start":{"date-parts":[[2023,2,1]],"date-time":"2023-02-01T00:00:00Z","timestamp":1675209600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["31872288"],"award-info":[{"award-number":["31872288"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["31970471"],"award-info":[{"award-number":["31970471"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,2,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>The Drosophila testis is an excellent system for studying the process from germ stem cells to motile sperm, including the proliferation of male germ cells, meiosis of primary spermatocytes, mitochondrial morphogenesis, and spermatid individualization. We previously demonstrated that ocnus (ocn) plays an essential role in male germ cell development. Among those genes and proteins whose expression levels were changed as a result of ocn knockdown, cytochrome c1-like (cyt-c1L) was downregulated significantly. Here, we show that cyt-c1L is highly expressed in the testis of D. melanogaster. Knockdown or mutation of cyt-c1L in early germ cells of flies resulted in male sterility. Immunofluorescence staining showed that cyt-c1L knockdown testes had no defects in early spermatogenesis; however, in late stages, in contrast to many individualization complexes (ICs) composed of F-actin cones that appeared at different positions in control testes, no actin cones or ICs were observed in cyt-c1L knockdown testes. Furthermore, no mature sperm were found in the seminal vesicle of cyt-c1L knockdown testes whereas the control seminal vesicle was full of mature sperm with needle-like nuclei. cyt-c1L knockdown also caused abnormal mitochondrial morphogenesis during spermatid elongation. Excessive apoptotic signals accumulated in the base of cyt-c1L knockdown fly testes. These results suggest that cyt-c1L may play an important role in spermatogenesis by affecting the mitochondrial morphogenesis and individualization of sperm in D. melanogaster.<\/jats:p>","DOI":"10.1242\/jeb.245277","type":"journal-article","created":{"date-parts":[[2023,1,16]],"date-time":"2023-01-16T06:25:01Z","timestamp":1673850301000},"source":"Crossref","is-referenced-by-count":10,"title":["<i>Cytochrome c1-like<\/i>\n                    is required for mitochondrial morphogenesis and individualization during spermatogenesis in\n                    <i>Drosophila melanogaster<\/i>"],"prefix":"10.1242","volume":"226","author":[{"given":"Meng-Yan","family":"Chen","sequence":"first","affiliation":[{"name":"School of Life Sciences, Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Central China Normal University , Wuhan 430079 , PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Duan","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Central China Normal University , Wuhan 430079 , PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Central China Normal University , Wuhan 430079 , PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mao-Jiu","family":"Ran","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Central China Normal University , Wuhan 430079 , PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Ai","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Central China Normal University , Wuhan 430079 , PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya","family":"Zheng","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Central China Normal University , Wuhan 430079 , PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1823-373X","authenticated-orcid":false,"given":"Yu-Feng","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Central China Normal University , Wuhan 430079 , PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2023,2,3]]},"reference":[{"key":"2023020309481702800_JEB245277C1","doi-asserted-by":"publisher","first-page":"687","DOI":"10.1016\/S1534-5807(03)00120-5","article-title":"Caspase activity and a specific cytochrome C are required for sperm differentiation in Drosophila","volume":"4","author":"Arama","year":"2003","journal-title":"Dev. 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