{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T12:50:09Z","timestamp":1775911809994,"version":"3.50.1"},"reference-count":54,"publisher":"The Company of Biologists","issue":"18","license":[{"start":{"date-parts":[[2023,9,22]],"date-time":"2023-09-22T00:00:00Z","timestamp":1695340800000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["R01NS114514"],"award-info":[{"award-number":["R01NS114514"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100007165","name":"University of Missouri","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100007165","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,9,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Breathing is generated by a rhythmic neural circuit in the brainstem, which contains conserved elements across vertebrate groups. In adult frogs, the \u2018lung area\u2019 located in the reticularis parvocellularis is thought to represent the core rhythm generator for breathing. Although this region is necessary for breathing-related motor output, whether it functions as an endogenous oscillator when isolated from other brainstem centers is not clear. Therefore, we generated thick brainstem sections that encompass the lung area to determine whether it can generate breathing-related motor output in a highly reduced preparation. Brainstem sections did not produce activity. However, subsaturating block of glycine receptors reliably led to the emergence of rhythmic motor output that was further enhanced by blockade of GABAA receptors. Output occurred in singlets and multi-burst episodes resembling the intact network. However, burst frequency was slower and individual bursts had longer durations than those produced by the intact preparation. In addition, burst frequency was reduced by noradrenaline and \u03bc-opioids, and increased by serotonin, as observed in the intact network and in vivo. These results suggest that the lung area can be activated to produce rhythmic respiratory-related motor output in a reduced brainstem section and provide new insights into respiratory rhythm generation in adult amphibians. First, clustering breaths into episodes can occur within the rhythm-generating network without long-range input from structures such as the pons. Second, local inhibition near, or within, the rhythmogenic center may need to be overridden to express the respiratory rhythm.<\/jats:p>","DOI":"10.1242\/jeb.245951","type":"journal-article","created":{"date-parts":[[2023,9,4]],"date-time":"2023-09-04T13:35:30Z","timestamp":1693834530000},"source":"Crossref","is-referenced-by-count":5,"title":["Activation of respiratory-related bursting in an isolated medullary section from adult bullfrogs"],"prefix":"10.1242","volume":"226","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7222-6047","authenticated-orcid":false,"given":"Sandy E.","family":"Saunders","sequence":"first","affiliation":[{"name":"Biological Sciences, University of Missouri , Columbia, MO 65211 , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joseph M.","family":"Santin","sequence":"additional","affiliation":[{"name":"Biological Sciences, University of Missouri , Columbia, MO 65211 , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[2023,9,22]]},"reference":[{"key":"2023092810004445800_JEB245951C1","doi-asserted-by":"publisher","first-page":"R105","DOI":"10.1152\/ajpregu.00209.2020","article-title":"Neuromodulation or energy failure? 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