{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T01:12:36Z","timestamp":1790730756548,"version":"4.1.0"},"reference-count":22,"publisher":"The Company of Biologists","issue":"4","license":[{"start":{"date-parts":[[2025,2,20]],"date-time":"2025-02-20T00:00:00Z","timestamp":1740009600000},"content-version":"vor","delay-in-days":5,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"National Science Foundation","award":["2131037"],"award-info":[{"award-number":["2131037"]}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["R33AT010408"],"award-info":[{"award-number":["R33AT010408"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100006686","name":"University of Miami","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100006686","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,2,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Drosophila\u2019s innate response to gravity, geotaxis, has been used to assess the impact of aging and disease on motor performance. Despite its rich history, fly geotaxis continues to be largely measured manually and assessed through simplistic metrics, limiting analytic insights into the behavior. Here, we have constructed a fully programmable apparatus and developed a multi-object tracking software capable of following sub-second movements of individual flies, thus allowing quantitative analysis of geotaxis. The apparatus monitors 10 fly cohorts simultaneously, with each cohort consisting of up to 7 flies. The software tracks single flies during the entire run with \u223c97% accuracy, yielding detailed climbing curve, speed and movement direction with 1\/30\u2005s resolution. Our tracking permits the construction of multi-variable metrics and the detection of transitory movement phenotypes, such as slips and falls. The platform is therefore poised to advance Drosophila geotaxis assay into a comprehensive assessment of locomotor behavior.<\/jats:p>","DOI":"10.1242\/jeb.248029","type":"journal-article","created":{"date-parts":[[2025,2,20]],"date-time":"2025-02-20T10:47:52Z","timestamp":1740048472000},"source":"Crossref","is-referenced-by-count":4,"title":["High-resolution, high-throughput analysis of <i>Drosophila<\/i> geotactic behavior"],"prefix":"10.1242","volume":"228","author":[{"given":"Tijana","family":"Canic","sequence":"first","affiliation":[{"name":"University of Miami 1 Department of Physics , , Coral Gables, FL 33146 ,","place":["USA"]},{"name":"University of Miami Miller School of Medicine 2 Department of Molecular and Cellular Pharmacology , , Miami, FL 33136 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Lopez","sequence":"additional","affiliation":[{"name":"University of Miami 1 Department of Physics , , Coral Gables, FL 33146 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalie","family":"Ortiz-Vega","sequence":"additional","affiliation":[{"name":"University of Miami Miller School of Medicine 2 Department of Molecular and Cellular Pharmacology , , Miami, FL 33136 ,","place":["USA"]},{"name":"University of Chicago 3 Department of Neurology , , Chicago, IL 60637 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7599-1430","authenticated-orcid":false,"given":"R. Grace","family":"Zhai","sequence":"additional","affiliation":[{"name":"University of Miami Miller School of Medicine 2 Department of Molecular and Cellular Pharmacology , , Miami, FL 33136 ,","place":["USA"]},{"name":"University of Chicago 3 Department of Neurology , , Chicago, IL 60637 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4642-6678","authenticated-orcid":false,"given":"Sheyum","family":"Syed","sequence":"additional","affiliation":[{"name":"University of Miami 1 Department of Physics , , Coral Gables, FL 33146 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2025,2,20]]},"reference":[{"key":"2025022010473718000_JEB248029C1","doi-asserted-by":"publisher","first-page":"222","DOI":"10.1111\/j.1601-183X.2005.00154.x","article-title":"Gravitaxis in Drosophila melanogaster: a forward genetic screen","volume":"5","author":"Armstrong","year":"2006","journal-title":"Genes Brain Behav."},{"key":"2025022010473718000_JEB248029C2","doi-asserted-by":"publisher","first-page":"859","DOI":"10.1242\/jeb.078758","article-title":"Dietary composition regulates Drosophila mobility and cardiac physiology","volume":"216","author":"Bazzell","year":"2013","journal-title":"J. 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Gerontol."},{"key":"2025022010473718000_JEB248029C9","doi-asserted-by":"publisher","first-page":"835","DOI":"10.1126\/science.134.3482.835","article-title":"Sign of taxis as a property of the genotype","volume":"134","author":"Hirsch","year":"1961","journal-title":"Science"},{"key":"2025022010473718000_JEB248029C10","doi-asserted-by":"publisher","first-page":"165","DOI":"10.1038\/nature07810","article-title":"The neural basis of Drosophila gravity-sensing and hearing","volume":"458","author":"Kamikouchi","year":"2009","journal-title":"Nature"},{"key":"2025022010473718000_JEB248029C11","doi-asserted-by":"publisher","first-page":"2023.03.08.531317","DOI":"10.1101\/2023.03.08.531317","article-title":"Drosophila antennae are dispensable for gravity orientation","author":"Kladt","year":"2023","journal-title":"bioRxiv"},{"key":"2025022010473718000_JEB248029C12","doi-asserted-by":"publisher","first-page":"755","DOI":"10.1039\/c0ib00149j","article-title":"The iFly tracking system for an automated locomotor and behavioural analysis of Drosophila melanogaster","volume":"3","author":"Kohlhoff","year":"2011","journal-title":"Integr. 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