{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T23:10:26Z","timestamp":1773184226360,"version":"3.50.1"},"reference-count":61,"publisher":"The Company of Biologists","issue":"21","license":[{"start":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T00:00:00Z","timestamp":1762473600000},"content-version":"vor","delay-in-days":6,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["NS086932"],"award-info":[{"award-number":["NS086932"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["P40-OD010440"],"award-info":[{"award-number":["P40-OD010440"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"name":"National Science Foundation","award":["IOS-1844657"],"award-info":[{"award-number":["IOS-1844657"]}]},{"DOI":"10.13039\/100006686","name":"University of Miami","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100006686","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,11,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Animals alter their behavior in response to changes in the environment. Upon encountering hyperosmotic conditions, the nematode worm Caenorhabditis elegans initiates avoidance and cessation of egg-laying behavior. While the sensory pathway for osmotic avoidance is well understood, less is known about how egg laying is inhibited. We analyzed egg-laying behavior after acute and chronic shifts to and from hyperosmotic media. Animals on 400\u2005mmol\u2005l\u22121 sorbitol stop laying eggs immediately but then resume \u223c3\u2005h later, after accumulating additional eggs in the uterus. Surprisingly, the hyperosmotic cessation of egg laying still occurred in known osmotic avoidance signaling mutants. Acute hyperosmotic shifts in hyperosmotic-resistant mutants overproducing glycerol also blocked egg laying, but these animals resumed egg laying more quickly than similarly treated wild-type animals. These results suggest that hyperosmotic conditions disrupt a \u2018high-inside\u2019 hydrostatic pressure gradient required for egg laying. Consistent with this hypothesis, animals adapted to hyperosmotic conditions laid more eggs after acute shifts back to normosmic conditions. Optogenetic stimulation of the HSN egg-laying command neurons in hyper-osmotic treated animals led to fewer and slower egg-laying events, an effect not seen following direct optogenetic stimulation of the postsynaptic vulval muscles. Hyperosmotic conditions also affected egg-laying circuit activity with the vulval muscles, showing reduced Ca2+ transient amplitudes and frequency even after egg-laying resumes. Together, these results indicate that hyperosmotic conditions regulate egg-laying via two mechanisms: a sensory pathway that acts to reduce HSN excitability and neurotransmitter release, and a biophysical mechanism where a hydrostatic pressure gradient reports egg accumulation in the uterus.<\/jats:p>","DOI":"10.1242\/jeb.250132","type":"journal-article","created":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T07:17:44Z","timestamp":1759735064000},"source":"Crossref","is-referenced-by-count":3,"title":["Osmolarity regulates\n                    <i>Caenorhabditis elegans<\/i>\n                    egg-laying behavior via chemosensory and biophysical mechanisms"],"prefix":"10.1242","volume":"228","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2773-3496","authenticated-orcid":false,"given":"Emmanuel","family":"Medrano","sequence":"first","affiliation":[{"name":"University of Miami Department of Biology , , 1301 Memorial Drive, Coral Gables, FL 33146 ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karen","family":"Jendrick","sequence":"additional","affiliation":[{"name":"University of Miami Department of Biology , , 1301 Memorial Drive, Coral Gables, FL 33146 ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julian","family":"McQuirter","sequence":"additional","affiliation":[{"name":"University of Miami Department of Biology , , 1301 Memorial Drive, Coral Gables, FL 33146 ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claire","family":"Moxham","sequence":"additional","affiliation":[{"name":"University of Miami Department of Biology , , 1301 Memorial Drive, Coral Gables, FL 33146 ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dominique","family":"Rajic","sequence":"additional","affiliation":[{"name":"University of Miami Department of Biology , , 1301 Memorial Drive, Coral Gables, FL 33146 ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lila","family":"Rosendorf","sequence":"additional","affiliation":[{"name":"University of Miami Department of Biology , , 1301 Memorial Drive, Coral Gables, FL 33146 ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liraz","family":"Stilman","sequence":"additional","affiliation":[{"name":"University of Miami Department of Biology , , 1301 Memorial Drive, Coral Gables, FL 33146 ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dontrel","family":"Wilright","sequence":"additional","affiliation":[{"name":"University of Miami Department of Biology , , 1301 Memorial Drive, Coral Gables, FL 33146 ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9930-0924","authenticated-orcid":false,"given":"Kevin M.","family":"Collins","sequence":"additional","affiliation":[{"name":"University of Miami Department of Biology , , 1301 Memorial Drive, Coral Gables, FL 33146 ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[2025,11,7]]},"reference":[{"key":"2025110705274385400_JEB250132C1","doi-asserted-by":"publisher","first-page":"247","DOI":"10.1016\/j.neuron.2005.02.024","article-title":"Tyramine functions independently of octopamine in the Caenorhabditis elegans nervous system","volume":"46","author":"Alkema","year":"2005","journal-title":"Neuron"},{"key":"2025110705274385400_JEB250132C2","doi-asserted-by":"publisher","first-page":"1512","DOI":"10.1101\/gad.14.12.1512","article-title":"daf-12 encodes a nuclear receptor that regulates the dauer diapause and developmental age in C. elegans","volume":"14","author":"Antebi","year":"2000","journal-title":"Genes Dev."},{"key":"2025110705274385400_JEB250132C3","first-page":"679","article-title":"24 feeding and defecation","volume":"33","author":"Avery","year":"1997","journal-title":"Cold Spring Harb. Monograph Archive"},{"key":"2025110705274385400_JEB250132C4","doi-asserted-by":"publisher","first-page":"e1006697","DOI":"10.1371\/journal.pgen.1006697","article-title":"Local neuropeptide signaling modulates serotonergic transmission to shape the temporal organization of C. elegans egg-laying behavior","volume":"13","author":"Banerjee","year":"2017","journal-title":"PLoS Genet."},{"key":"2025110705274385400_JEB250132C5","doi-asserted-by":"crossref","DOI":"10.1895\/wormbook.1.123.1","volume-title":"Chemosensation in C. elegans","author":"Bargmann","year":"2006"},{"key":"2025110705274385400_JEB250132C6","doi-asserted-by":"publisher","first-page":"1805","DOI":"10.1093\/genetics\/165.4.1805","article-title":"Caenorhabditis elegans Galphaq regulates egg-laying behavior via a PLCbeta-independent and serotonin-dependent signaling pathway and likely functions both in the nervous system and in muscle","volume":"165","author":"Bastiani","year":"2003","journal-title":"Genetics"},{"key":"2025110705274385400_JEB250132C7","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1093\/genetics\/77.1.71","article-title":"The Genetics of Caenorhabditis elegans","volume":"77","author":"Brenner","year":"1974","journal-title":"Genetics"},{"key":"2025110705274385400_JEB250132C8","first-page":"1","volume-title":"Assaying Mechanosensation","author":"Chalfie","year":"2014"},{"key":"2025110705274385400_JEB250132C9","doi-asserted-by":"publisher","first-page":"316","DOI":"10.3389\/fgene.2015.00316","article-title":"Sorbitol treatment extends lifespan and induces the osmotic stress response in Caenorhabditis elegans","volume":"6","author":"Chandler-Brown","year":"2015","journal-title":"Front. Genet."},{"key":"2025110705274385400_JEB250132C10","doi-asserted-by":"publisher","first-page":"305","DOI":"10.1016\/S0076-6879(04)89018-9","article-title":"Genetic analysis of RGS protein function in Caenorhabditis elegans","volume":"389","author":"Chase","year":"2004","journal-title":"Methods Enzymol."},{"key":"2025110705274385400_JEB250132C11","doi-asserted-by":"publisher","first-page":"55","DOI":"10.2174\/1875036201206010055","article-title":"Primer1: primer design web service for tetra-primer ARMS-PCR","volume":"6","author":"Collins","year":"2012","journal-title":"The Open Bioinformatics Journal"},{"key":"2025110705274385400_JEB250132C12","doi-asserted-by":"publisher","first-page":"761","DOI":"10.1523\/JNEUROSCI.3896-12.2013","article-title":"Postsynaptic ERG potassium channels limit muscle excitability to allow distinct egg-laying behavior states in Caenorhabditis elegans","volume":"33","author":"Collins","year":"2013","journal-title":"J. Neurosci."},{"key":"2025110705274385400_JEB250132C13","doi-asserted-by":"publisher","first-page":"e21126","DOI":"10.7554\/eLife.21126","article-title":"Activity of the C. elegans egg-laying behavior circuit is controlled by competing activation and feedback inhibition","volume":"5","author":"Collins","year":"2016","journal-title":"eLife"},{"key":"2025110705274385400_JEB250132C14","doi-asserted-by":"publisher","first-page":"243","DOI":"10.1093\/genetics\/90.2.243","article-title":"Osmotic avoidance defective mutants of the nematode Caenorhabditis elegans","volume":"90","author":"Culotti","year":"1978","journal-title":"Genetics"},{"key":"2025110705274385400_JEB250132C15","doi-asserted-by":"publisher","first-page":"iyac084","DOI":"10.1093\/genetics\/iyac084","article-title":"Serotonin signals through postsynaptic G\u03b1q, Trio RhoGEF, and diacylglycerol to promote Caenorhabditis elegans egg-laying circuit activity and behavior","volume":"221","author":"Dhakal","year":"2022","journal-title":"Genetics"},{"key":"2025110705274385400_JEB250132C16","doi-asserted-by":"publisher","first-page":"249","DOI":"10.1016\/S0896-6273(01)00587-6","article-title":"Regulation of retrograde signaling at neuromuscular junctions by the novel C2 domain protein AEX-1","volume":"33","author":"Doi","year":"2002","journal-title":"Neuron"},{"key":"2025110705274385400_JEB250132C17","doi-asserted-by":"publisher","first-page":"16285","DOI":"10.1523\/JNEUROSCI.2667-12.2012","article-title":"IRK-1 potassium channels mediate peptidergic inhibition of Caenorhabditis elegans serotonin neurons via a go signaling pathway","volume":"32","author":"Emtage","year":"2012","journal-title":"J. Neurosci."},{"key":"2025110705274385400_JEB250132C18","doi-asserted-by":"publisher","first-page":"1272","DOI":"10.1016\/j.neuron.2018.12.001","article-title":"Synaptic communication upon gentle touch","volume":"100","author":"Fechner","year":"2018","journal-title":"Neuron"},{"key":"2025110705274385400_JEB250132C19","doi-asserted-by":"publisher","first-page":"353","DOI":"10.1016\/j.neuron.2007.11.030","article-title":"GFP Reconstitution Across Synaptic Partners (GRASP) defines cell contacts and synapses in living nervous systems","volume":"57","author":"Feinberg","year":"2008","journal-title":"Neuron"},{"key":"2025110705274385400_JEB250132C20","doi-asserted-by":"publisher","first-page":"E3525","DOI":"10.1073\/pnas.1423808112","article-title":"Environmental CO2 inhibits Caenorhabditis elegans egg-laying by modulating olfactory neurons and evokes widespread changes in neural activity","volume":"112","author":"Fenk","year":"2015","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2025110705274385400_JEB250132C21","doi-asserted-by":"publisher","first-page":"127","DOI":"10.1111\/j.1474-9726.2006.00203.x","article-title":"The nuclear hormone receptor DAF-12 has opposing effects on Caenorhabditis elegans lifespan and regulates genes repressed in multiple long-lived worms","volume":"5","author":"Fisher","year":"2006","journal-title":"Aging Cell"},{"key":"2025110705274385400_JEB250132C22","doi-asserted-by":"publisher","first-page":"1887","DOI":"10.1016\/j.bpj.2015.03.020","article-title":"Worms under pressure: bulk mechanical properties of C. elegans are independent of the cuticle","volume":"108","author":"Gilpin","year":"2015","journal-title":"Biophys. J."},{"key":"2025110705274385400_JEB250132C23","doi-asserted-by":"crossref","DOI":"10.1895\/wormbook.1.62.1","volume-title":"Mechanosensation","author":"Goodman","year":"2006"},{"key":"2025110705274385400_JEB250132C24","doi-asserted-by":"publisher","first-page":"1359","DOI":"10.1534\/genetics.112.142125","article-title":"Receptors and other signaling proteins required for serotonin control of locomotion in Caenorhabditis elegans","volume":"192","author":"G\u00fcrel","year":"2012","journal-title":"Genetics"},{"key":"2025110705274385400_JEB250132C25","doi-asserted-by":"publisher","first-page":"116","DOI":"10.1242\/jeb.34.1.116","article-title":"Structure and function in the nematodes: internal pressure and cuticular structure in Ascaris","volume":"34","author":"Harris","year":"1957","journal-title":"J. Exp. Biol."},{"key":"2025110705274385400_JEB250132C26","doi-asserted-by":"publisher","first-page":"1952","DOI":"10.1523\/JNEUROSCI.19-06-01952.1999","article-title":"Distinct signaling pathways mediate touch and osmosensory responses in a polymodal sensory neuron","volume":"19","author":"Hart","year":"1999","journal-title":"J. Neurosci."},{"key":"2025110705274385400_JEB250132C27","doi-asserted-by":"publisher","first-page":"C1867","DOI":"10.1152\/ajpcell.00514.2006","article-title":"Functional analysis of the aquaporin gene family in Caenorhabditis elegans","volume":"292","author":"Huang","year":"2007","journal-title":"Am. J. Physiol. Cell Physiol."},{"key":"2025110705274385400_JEB250132C28","doi-asserted-by":"publisher","first-page":"4430","DOI":"10.1016\/j.cub.2023.08.088","article-title":"A single neuron in C. elegans orchestrates multiple motor outputs through parallel modes of transmission","volume":"33","author":"Huang","year":"2023","journal-title":"Curr. Biol."},{"key":"2025110705274385400_JEB250132C29","doi-asserted-by":"publisher","first-page":"93","DOI":"10.1534\/genetics.106.065516","article-title":"A specific subset of transient receptor potential vanilloid-type channel subunits in Caenorhabditis elegans endocrine cells function as mixed heteromers to promote neurotransmitter release","volume":"175","author":"Jose","year":"2007","journal-title":"Genetics"},{"key":"2025110705274385400_JEB250132C30","doi-asserted-by":"publisher","first-page":"2227","DOI":"10.1073\/pnas.90.6.2227","article-title":"A dual mechanosensory and chemosensory neuron in Caenorhabditis elegans","volume":"90","author":"Kaplan","year":"1993","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2025110705274385400_JEB250132C31","doi-asserted-by":"publisher","first-page":"3635","DOI":"10.1523\/JNEUROSCI.2150-20.2021","article-title":"The sex-specific VC neurons are mechanically activated motor neurons that facilitate serotonin-induced egg laying in C. elegans","volume":"41","author":"Kopchock","year":"2021","journal-title":"J. Neurosci."},{"key":"2025110705274385400_JEB250132C32","doi-asserted-by":"publisher","first-page":"C785","DOI":"10.1152\/ajpcell.00381.2003","article-title":"Adaptation of the nematode Caenorhabditis elegans to extreme osmotic stress","volume":"286","author":"Lamitina","year":"2004","journal-title":"Am. J. Physiol. Cell Physiol."},{"key":"2025110705274385400_JEB250132C33","doi-asserted-by":"publisher","first-page":"14531","DOI":"10.1073\/pnas.2235619100","article-title":"Mammalian TRPV4 (VR-OAC) directs behavioral responses to osmotic and mechanical stimuli in Caenorhabditis elegans","volume":"100","author":"Liedtke","year":"2003","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"2025110705274385400_JEB250132C34","doi-asserted-by":"publisher","first-page":"2330","DOI":"10.1016\/j.cub.2023.05.008","article-title":"Muscle-directed mechanosensory feedback activates egg-laying circuit activity and behavior in Caenorhabditis elegans","volume":"33","author":"Medrano","year":"2023","journal-title":"Curr. Biol."},{"key":"2025110705274385400_JEB250132C35","doi-asserted-by":"publisher","first-page":"RP88253","DOI":"10.7554\/eLife.88253","article-title":"Natural variation in the Caenorhabditis elegans egg-laying circuit modulates an intergenerational fitness trade-off","volume":"12","author":"Mignerot","year":"2024","journal-title":"eLife"},{"key":"2025110705274385400_JEB250132C36","doi-asserted-by":"publisher","first-page":"3789","DOI":"10.1523\/JNEUROSCI.2301-22.2023","article-title":"Multiple subthreshold GPCR signals combined by the G proteins G\u03b1q and G\u03b1s activate the C. elegans egg-laying muscles","volume":"43","author":"Olson","year":"2023","journal-title":"J. Neurosci."},{"key":"2025110705274385400_JEB250132C37","doi-asserted-by":"publisher","first-page":"3361","DOI":"10.1242\/dev.127.15.3361","article-title":"The homeodomain protein CePHOX2\/CEH-17 controls antero-posterior axonal growth in C. elegans","volume":"127","author":"Pujol","year":"2000","journal-title":"Development"},{"key":"2025110705274385400_JEB250132C38","doi-asserted-by":"publisher","first-page":"56911","DOI":"10.3791\/56911","article-title":"Ratiometric calcium imaging of individual neurons in behaving Caenorhabditis elegans","volume":"132","author":"Ravi","year":"2018","journal-title":"J. Vis. Exp."},{"key":"2025110705274385400_JEB250132C39","doi-asserted-by":"publisher","first-page":"6283","DOI":"10.1523\/JNEUROSCI.3658-17.2018","article-title":"Homeostatic feedback modulates the development of two-state patterned activity in a model serotonin motor circuit in Caenorhabditis elegans","volume":"38","author":"Ravi","year":"2018","journal-title":"J. Neurosci."},{"key":"2025110705274385400_JEB250132C40","doi-asserted-by":"publisher","first-page":"iyab080","DOI":"10.1093\/genetics\/iyab080","article-title":"Presynaptic G\u03b1o (GOA-1) signals to depress command neuron excitability and allow stretch-dependent modulation of egg laying in Caenorhabditis elegans","volume":"218","author":"Ravi","year":"2021","journal-title":"Genetics"},{"key":"2025110705274385400_JEB250132C41","doi-asserted-by":"publisher","first-page":"1168","DOI":"10.1038\/nn.2186","article-title":"FMRFamide neuropeptides and acetylcholine synergistically inhibit egg-laying by C. elegans","volume":"11","author":"Ringstad","year":"2008","journal-title":"Nat. Neurosci."},{"key":"2025110705274385400_JEB250132C42","doi-asserted-by":"publisher","first-page":"55","DOI":"10.1016\/S0896-6273(00)80434-1","article-title":"The G\u03b1 protein ODR-3 mediates olfactory and nociceptive function and controls cilium morphogenesis in C. elegans olfactory neurons","volume":"20","author":"Roayaie","year":"1998","journal-title":". Neuron"},{"key":"2025110705274385400_JEB250132C43","doi-asserted-by":"publisher","first-page":"1069","DOI":"10.1093\/genetics\/156.3.1069","article-title":"Calcium\/calmodulin-dependent protein kinase II regulates Caenorhabditis elegans locomotion in concert with a Go\/Gq signaling network","volume":"156","author":"Robatzek","year":"2000","journal-title":"Genetics"},{"key":"2025110705274385400_JEB250132C44","doi-asserted-by":"publisher","first-page":"e1001267","DOI":"10.1371\/journal.pgen.1001267","article-title":"The Caenorhabditis elegans mucin-like protein OSM-8 negatively regulates osmosensitive physiology via the transmembrane protein PTR-23","volume":"7","author":"Rohlfing","year":"2011","journal-title":"PLoS Genet."},{"key":"2025110705274385400_JEB250132C45","unstructured":"Sawin, E. R.\n           (1996). Genetic and cellular analysis of modulated behaviors in Caenorhabditis elegans. PhD dissertation, Massachusetts Institute of Technology."},{"key":"2025110705274385400_JEB250132C46","doi-asserted-by":"publisher","first-page":"619","DOI":"10.1016\/S0896-6273(00)81199-X","article-title":"C. elegans locomotory rate is modulated by the environment through a dopaminergic pathway and by experience through a serotonergic pathway","volume":"26","author":"Sawin","year":"2000","journal-title":"Neuron"},{"key":"2025110705274385400_JEB250132C47","doi-asserted-by":"publisher","first-page":"1648","DOI":"10.1126\/science.7886454","article-title":"Modulation of serotonin-controlled behaviors by go in Caenorhabditis elegans","volume":"267","author":"S\u00e9galat","year":"1995","journal-title":"Science"},{"key":"2025110705274385400_JEB250132C48","doi-asserted-by":"publisher","first-page":"536","DOI":"10.1126\/science.1242958","article-title":"Mating induces shrinking and death in Caenorhabditis mothers","volume":"343","author":"Shi","year":"2014","journal-title":"Science"},{"key":"2025110705274385400_JEB250132C49","doi-asserted-by":"publisher","first-page":"1015","DOI":"10.1523\/JNEUROSCI.3107-04.2005","article-title":"Polymodal sensory function of the Caenorhabditis elegans OCR-2 channel arises from distinct intrinsic determinants within the protein and is selectively conserved in mammalian TRPV proteins","volume":"25","author":"Sokolchik","year":"2005","journal-title":"J. Neurosci."},{"key":"2025110705274385400_JEB250132C50","doi-asserted-by":"publisher","first-page":"161","DOI":"10.1534\/genetics.167.1.161","article-title":"Caenorhabditis elegans OSR-1 regulates behavioral and physiological responses to hyperosmotic environments","volume":"167","author":"Solomon","year":"2004","journal-title":"Genetics"},{"key":"2025110705274385400_JEB250132C51","doi-asserted-by":"publisher","first-page":"411","DOI":"10.1016\/S1097-2765(00)80436-0","article-title":"The RFX-type transcription factor DAF-19 regulates sensory neuron cilium formation in C. elegans","volume":"5","author":"Swoboda","year":"2000","journal-title":"Mol. Cell"},{"key":"2025110705274385400_JEB250132C52","doi-asserted-by":"publisher","first-page":"307","DOI":"10.1016\/S0896-6273(02)00757-2","article-title":"Combinatorial expression of TRPV channel proteins defines their sensory functions and subcellular localization in C. elegans neurons","volume":"35","author":"Tobin","year":"2002","journal-title":"Neuron"},{"key":"2025110705274385400_JEB250132C53","doi-asserted-by":"publisher","first-page":"203","DOI":"10.1016\/S0896-6273(00)80527-9","article-title":"Control of alternative behavioral states by serotonin in Caenorhabditis elegans","volume":"21","author":"Waggoner","year":"1998","journal-title":"Neuron"},{"key":"2025110705274385400_JEB250132C54","doi-asserted-by":"publisher","first-page":"1327","DOI":"10.1534\/genetics.106.059089","article-title":"Identification of a novel gene family involved in osmotic stress response in Caenorhabditis elegans","volume":"174","author":"Wheeler","year":"2006","journal-title":"Genetics"},{"key":"2025110705274385400_JEB250132C55","doi-asserted-by":"publisher","first-page":"e0678242024","DOI":"10.1523\/JNEUROSCI.0678-24.2024","article-title":"The C. elegans uv1 neuroendocrine cells provide mechanosensory feedback of vulval opening","volume":"45","author":"Yan","year":"2025","journal-title":"J. Neurosci."},{"key":"2025110705274385400_JEB250132C56","doi-asserted-by":"publisher","first-page":"E88","DOI":"10.1093\/nar\/29.17.e88","article-title":"An efficient procedure for genotyping single nucleotide polymorphisms","volume":"29","author":"Ye","year":"2001","journal-title":"Nucleic Acids Res."},{"key":"2025110705274385400_JEB250132C57","doi-asserted-by":"publisher","first-page":"ENEURO.0282-16.2017","DOI":"10.1523\/ENEURO.0282-16.2017","article-title":"An aversive response to osmotic upshift in Caenorhabditis elegans","volume":"4","author":"Yu","year":"2017","journal-title":"eNeuro"},{"key":"2025110705274385400_JEB250132C58","doi-asserted-by":"publisher","first-page":"1445","DOI":"10.1016\/j.cub.2008.08.047","article-title":"A self-regulating feed-forward circuit controlling C. elegans egg-laying behavior","volume":"18","author":"Zhang","year":"2008","journal-title":"Curr. Biol."},{"key":"2025110705274385400_JEB250132C59","doi-asserted-by":"publisher","first-page":"81","DOI":"10.1186\/1752-0509-4-81","article-title":"A circuit model of the temporal pattern generator of Caenorhabditis egg-laying behavior","volume":"9","author":"Zhang","year":"2010","journal-title":"BMC Syst. Biol."},{"key":"2025110705274385400_JEB250132C60","doi-asserted-by":"publisher","first-page":"366","DOI":"10.1111\/ele.13925","article-title":"Directed movement changes coexistence outcomes in heterogeneous environments","volume":"25","author":"Zhang","year":"2022","journal-title":"Ecol. Lett."},{"key":"2025110705274385400_JEB250132C61","doi-asserted-by":"publisher","first-page":"2797","DOI":"10.1016\/j.cub.2014.09.076","article-title":"Egg-laying demand induces aversion of UV light in Drosophila females","volume":"24","author":"Zhu","year":"2014","journal-title":"Curr. Biol."}],"container-title":["Journal of Experimental Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.biologists.com\/jeb\/article-pdf\/doi\/10.1242\/jeb.250132\/3667842\/jeb250132.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/journals.biologists.com\/jeb\/article-pdf\/doi\/10.1242\/jeb.250132\/3672451\/jeb250132.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.biologists.com\/jeb\/article-pdf\/doi\/10.1242\/jeb.250132\/3672451\/jeb250132.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T10:27:50Z","timestamp":1762511270000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.biologists.com\/jeb\/article\/228\/21\/jeb250132\/369752\/Osmolarity-regulates-Caenorhabditis-elegans-egg"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,1]]},"references-count":61,"journal-issue":{"issue":"21","published-print":{"date-parts":[[2025,11,1]]}},"URL":"https:\/\/doi.org\/10.1242\/jeb.250132","relation":{},"ISSN":["0022-0949","1477-9145"],"issn-type":[{"value":"0022-0949","type":"print"},{"value":"1477-9145","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,1]]},"article-number":"jeb250132"}}