{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T00:43:25Z","timestamp":1770079405918,"version":"3.49.0"},"reference-count":93,"publisher":"The Company of Biologists","issue":"2","license":[{"start":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T00:00:00Z","timestamp":1769990400000},"content-version":"vor","delay-in-days":18,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"crossref","award":["BB\/N005740\/1"],"award-info":[{"award-number":["BB\/N005740\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000770","name":"University of Manchester","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100000770","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,1,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>The effect of global warming on rising aquatic temperatures is producing ever-steeper thermoclines. Fish encountering these sharp changes in water temperature might experience an acute-warming stress. Temperature is the most dominant environmental factor affecting heart function in fish, and without compensatory mechanisms as temperatures rise (e.g. higher heart rate), it could imperil cardiovascular performance. To enhance heart function during acute warming, fish release adrenaline to boost Ca2+ influx in heart cells (cardiomyocytes). However, the relationship between acute warming, elevated heart rate, adrenergic stimulation and intracellular Ca2+ handling is not well understood at the cellular level. In this study, we investigated the interplay between these key functional drivers in isolated ventricular cardiomyocytes of rainbow trout, at either their acclimation temperature of 10\u00b0C or following acute warming (22\u00b0C). A subset of cardiomyocytes from each group was treated with adrenaline, sarcoplasmic reticulum (SR) inhibitors (that inhibit intracellular Ca2+ cycling via the SR) or both, whereas pacing frequency was simultaneously increased (simulating faster heart rate). Using epifluorescent microscopy, we measured intracellular Ca2+ transients (\u0394[Ca2+]i) and Ca2+-cycling kinetics. Across all pacing frequencies, we found no differences in \u0394[Ca2+]i between control (untreated) 10\u00b0C and 22\u00b0C cardiomyocytes, and that adrenaline had a positive inotropic effect at both temperatures, but was less effective at 22\u00b0C. SR inhibition had no effect on \u0394[Ca2+]i, but was associated with a greater incidence of irregular \u0394[Ca2+]i. Our data suggest that acute thermal stress can disrupt Ca2+-homeostatic mechanisms in trout cardiomyocytes, potentially disrupting whole-heart contractility as global temperatures rise.<\/jats:p>","DOI":"10.1242\/jeb.251460","type":"journal-article","created":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T08:50:50Z","timestamp":1764579050000},"source":"Crossref","is-referenced-by-count":0,"title":["Thermal plasticity of adrenaline-mediated, frequency-dependent calcium homeostasis in rainbow trout ventricular cardiomyocytes"],"prefix":"10.1242","volume":"229","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9243-7055","authenticated-orcid":false,"given":"Ilan M.","family":"Ruhr","sequence":"first","affiliation":[{"name":"Biomedical Research and Innovation Centre, School of Science, Engineering and Environment, University of Salford 1 , Salford M5 4NT ,","place":["UK"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1023-915X","authenticated-orcid":false,"given":"Gina L. J.","family":"Galli","sequence":"additional","affiliation":[{"name":"University of Manchester 2 Division of Cardiovascular Sciences, Faculty of Biology, Medicine and Health , , Manchester M13 9NT ,","place":["UK"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5223-5205","authenticated-orcid":false,"given":"Holly A.","family":"Shiels","sequence":"additional","affiliation":[{"name":"University of Manchester 2 Division of Cardiovascular Sciences, Faculty of Biology, Medicine and Health , , Manchester M13 9NT ,","place":["UK"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[2026,2,2]]},"reference":[{"key":"2026020208264063600_JEB251460C1","doi-asserted-by":"publisher","first-page":"525","DOI":"10.1242\/jeb.201.4.525","article-title":"Ca2+-ATPase activity and Ca2+ uptake by sarcoplasmic reticulum in fish heart: effects of thermal acclimation","volume":"201","author":"Aho","year":"1998","journal-title":"J. Exp. Biol."},{"key":"2026020208264063600_JEB251460C2","doi-asserted-by":"publisher","first-page":"173","DOI":"10.1007\/s003600000171","article-title":"Cold acclimation increases basal heart rate but decreases its thermal tolerance in rainbow trout (Oncorhynchus mykiss)","volume":"171","author":"Aho","year":"2001","journal-title":"J. Comp. Physiol. B Biochem. Syst. Environ. Physiol."},{"key":"2026020208264063600_JEB251460C3","doi-asserted-by":"publisher","first-page":"R168","DOI":"10.1152\/ajpregu.00127.2012","article-title":"Effect of cold acclimation on troponin I isoform expression in striated muscle of rainbow trout","volume":"303","author":"Alderman","year":"2012","journal-title":"Am. J. Physiol. Regul. Integr. Comp. Physiol."},{"key":"2026020208264063600_JEB251460C4","doi-asserted-by":"publisher","first-page":"103","DOI":"10.1016\/j.jtherbio.2003.12.001","article-title":"Effects of temperature and calcium availability on ventricular myocardium from the neotropical teleost Piaractus mesopotamicus (Holmberg 1887 - Teleostei, Serrasalmidae)","volume":"29","author":"Anelli","year":"2004","journal-title":"J. Therm. Biol."},{"key":"2026020208264063600_JEB251460C5","doi-asserted-by":"publisher","first-page":"1495","DOI":"10.1242\/jeb.138347","article-title":"Effects of seasonal acclimatization on temperature-dependence of cardiac excitability in the roach, Rutilus rutilus","volume":"219","author":"Badr","year":"2016","journal-title":"J. Exp. Biol."},{"key":"2026020208264063600_JEB251460C6","doi-asserted-by":"publisher","first-page":"196","DOI":"10.1139\/f53-015","article-title":"Upper lethal temperatures of some British Columbia freshwater fishes","volume":"10","author":"Black","year":"1953","journal-title":"J. Fish. Res. Board Can."},{"key":"2026020208264063600_JEB251460C7","doi-asserted-by":"publisher","first-page":"309","DOI":"10.1093\/acprof:oso\/9780199672547.003.0014","article-title":"Linear and generalized linear mixed models","volume-title":"Ecological Statistics: Contemporary Theory and Application","author":"Bolker","year":"2015"},{"key":"2026020208264063600_JEB251460C8","doi-asserted-by":"publisher","first-page":"1775","DOI":"10.1007\/s00424-013-1312-2","article-title":"Mechanisms of Ca2+ handling in zebrafish ventricular myocytes","volume":"465","author":"Bovo","year":"2013","journal-title":"Pfl\u00fcgers Arch Eur. J. Physiol."},{"key":"2026020208264063600_JEB251460C9","doi-asserted-by":"publisher","first-page":"99","DOI":"10.1093\/icb\/11.1.99","article-title":"Energetic responses of salmon to temperature. A study of some thermal relations in the physiology and freshwater ecology of sockeye salmon (Oncorhynchus nerka)","volume":"11","author":"Brett","year":"1971","journal-title":"Am. Zool."},{"key":"2026020208264063600_JEB251460C10","doi-asserted-by":"publisher","first-page":"439","DOI":"10.1006\/jfbi.2000.1461","article-title":"Assessment of cardiac output as a predictor of metabolic rate in rainbow trout","volume":"58","author":"Brodeur","year":"2001","journal-title":"J. Fish Biol."},{"key":"2026020208264063600_JEB251460C11","doi-asserted-by":"publisher","first-page":"562","DOI":"10.3389\/fphys.2016.00562","article-title":"Myofilament calcium sensitivity: role in regulation of in vivo cardiac contraction and relaxation","volume":"7","author":"Chung","year":"2016","journal-title":"Front. Physiol."},{"key":"2026020208264063600_JEB251460C12","doi-asserted-by":"publisher","first-page":"R1631","DOI":"10.1152\/ajpregu.90461.2008","article-title":"Circulatory limits to oxygen supply during an acute temperature increase in the Chinook salmon (Oncorhynchus tshawytscha)","volume":"295","author":"Clark","year":"2008","journal-title":"Am. J. Physiol. Regul. Integr. Comp. Physiol."},{"key":"2026020208264063600_JEB251460C13","doi-asserted-by":"publisher","first-page":"R1493","DOI":"10.1152\/ajpregu.00127.2014","article-title":"The calcium stored in the sarcoplasmic reticulum acts as a safety mechanism in rainbow trout heart","volume":"307","author":"Cros","year":"2014","journal-title":"Am. J. Physiol. Regul. Integr. Comp. Physiol."},{"key":"2026020208264063600_JEB251460C14","doi-asserted-by":"publisher","first-page":"789","DOI":"10.1111\/j.1095-8649.2011.03076.x","article-title":"Temperature dependence of cardiac sarcoplasmic reticulum Ca2+-ATPase from rainbow trout, Oncorhynchus mykiss","volume":"79","author":"Da Silva","year":"2011","journal-title":"J. Fish Biol."},{"key":"2026020208264063600_JEB251460C15","doi-asserted-by":"publisher","first-page":"221","DOI":"10.1152\/physrev.1994.74.1.221","article-title":"Energy metabolism and contractility in ectothermic vertebrate hearts: hypoxia, acidosis, and low temperature","volume":"74","author":"Driedzic","year":"1994","journal-title":"Physiol. Rev."},{"key":"2026020208264063600_JEB251460C16","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1016\/j.jtherbio.2014.06.002","article-title":"Effects of autonomic blockade on acute thermal tolerance and cardioventilatory performance in rainbow trout, Oncorhynchus mykiss","volume":"44","author":"Ekstr\u00f6m","year":"2014","journal-title":"J. Therm. Biol."},{"key":"2026020208264063600_JEB251460C17","doi-asserted-by":"publisher","first-page":"1106","DOI":"10.1242\/jeb.134312","article-title":"Dynamic changes in scope for heart rate and cardiac autonomic control during warm acclimation in rainbow trout","volume":"219","author":"Ekstr\u00f6m","year":"2016","journal-title":"J. Exp. Biol."},{"key":"2026020208264063600_JEB251460C18","doi-asserted-by":"publisher","first-page":"757","DOI":"10.1007\/s00360-019-01243-7","article-title":"Can't beat the heat? Importance of cardiac control and coronary perfusion for heat tolerance in rainbow trout","volume":"189","author":"Ekstr\u00f6m","year":"2019","journal-title":"J. Comp. Physiol. B"},{"key":"2026020208264063600_JEB251460C19","first-page":"235","article-title":"Temperature and the cardiovascular system","volume-title":"Fish Physiology, Vol. 36B: The Cardiovascular System - Development, Plasticity, and Physiological Responses","author":"Eliason","year":"2017"},{"key":"2026020208264063600_JEB251460C20","doi-asserted-by":"publisher","first-page":"109","DOI":"10.1126\/science.1199158","article-title":"Differences in thermal tolerance among sockeye salmon populations","volume":"332","author":"Eliason","year":"2011","journal-title":"Science"},{"key":"2026020208264063600_JEB251460C21","doi-asserted-by":"publisher","first-page":"cot008","DOI":"10.1093\/conphys\/cot008","article-title":"Cardiorespiratory collapse at high temperature in swimming adult sockeye salmon","volume":"1","author":"Eliason","year":"2013","journal-title":"Conserv Physiol."},{"key":"2026020208264063600_JEB251460C22","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/bs.fp.2017.07.001","article-title":"Cardiac form, function, and physiology","volume-title":"Fish Physiology, Vol. 36A: The Cardiovascular System - Morphology, Control, and Function","author":"Farrell","year":"2017"},{"key":"2026020208264063600_JEB251460C23","doi-asserted-by":"publisher","first-page":"663","DOI":"10.1242\/jeb.199.3.663","article-title":"Maximum cardiac performance of rainbow trout (Oncorhynchus mykiss) at temperatures approaching their upper lethal limit","volume":"199","author":"Farrell","year":"1996","journal-title":"J. Exp. Biol."},{"key":"2026020208264063600_JEB251460C24","doi-asserted-by":"publisher","first-page":"835","DOI":"10.1139\/Z09-092","article-title":"Fish cardiorespiratory physiology in an era of climate change","volume":"87","author":"Farrell","year":"2009","journal-title":"Can. J. Zool."},{"key":"2026020208264063600_JEB251460C25","doi-asserted-by":"publisher","first-page":"4251","DOI":"10.1242\/jeb.087130","article-title":"Thermal dependence of cardiac function in arctic fish: implications of a warming world","volume":"216","author":"Franklin","year":"2013","journal-title":"J. Exp. Biol."},{"key":"2026020208264063600_JEB251460C26","doi-asserted-by":"publisher","first-page":"280","DOI":"10.1086\/597484","article-title":"Temperature sensitivity of cardiac function in pelagic fishes with different vertical mobilities: yellowfin tuna (Thunnus albacares), bigeye tuna (Thunnus obesus), mahimahi (Coryphaena hippurus), and swordfish (Xiphias gladius)","volume":"82","author":"Galli","year":"2009","journal-title":"Physiol. Biochem. Zool."},{"key":"2026020208264063600_JEB251460C27","doi-asserted-by":"publisher","first-page":"1068","DOI":"10.1242\/jeb.048231","article-title":"Temperature effects on Ca2+ cycling in scombrid cardiomyocytes: a phylogenetic comparison","volume":"214","author":"Galli","year":"2011","journal-title":"J. Exp. Biol."},{"key":"2026020208264063600_JEB251460C28","doi-asserted-by":"publisher","first-page":"7","DOI":"10.1016\/j.jtherbio.2010.08.007","article-title":"Elevated temperature, per se, does not limit the ability of rainbow trout to increase stroke volume","volume":"36","author":"Gamperl","year":"2011","journal-title":"J. Therm. Biol."},{"key":"2026020208264063600_JEB251460C29","doi-asserted-by":"publisher","first-page":"329","DOI":"10.1016\/s1095-6433(99)00126-9","article-title":"Modulation of stress hormones in rainbow trout by means of anesthesia, sensory deprivation and receptor blockade","volume":"124","author":"Gerwick","year":"1999","journal-title":"Comp. Biochem. Physiol. A Mol. Integr. Physiol."},{"key":"2026020208264063600_JEB251460C30","doi-asserted-by":"publisher","first-page":"194365","DOI":"10.1242\/jeb.194365","article-title":"Autonomic cardiac regulation facilitates acute heat tolerance in rainbow trout: in situ and in vivo support","volume":"222","author":"Gilbert","year":"2019","journal-title":"J. Exp. Biol."},{"key":"2026020208264063600_JEB251460C31","doi-asserted-by":"publisher","first-page":"coaa036","DOI":"10.1093\/conphys\/coaa036","article-title":"The thermal limits of cardiorespiratory performance in anadromous Arctic char (Salvelinus alpinus): a field-based investigation using a remote mobile laboratory","volume":"8","author":"Gilbert","year":"2020","journal-title":"Conserv. Physiol."},{"key":"2026020208264063600_JEB251460C32","doi-asserted-by":"publisher","first-page":"763","DOI":"10.1016\/s1095-6433(02)00046-6","article-title":"Beating the cold: the functional evolution of troponin C in teleost fish","volume":"132","author":"Gillis","year":"2002","journal-title":"Comp. Biochem. Physiol. A Mol. Integr. Physiol."},{"key":"2026020208264063600_JEB251460C33","doi-asserted-by":"publisher","first-page":"R1707","DOI":"10.1152\/ajpregu.2000.279.5.R1707","article-title":"Ca2+ binding to cardiac troponin C: effects of temperature and pH on mammalian and salmonid isoforms","volume":"279","author":"Gillis","year":"2000","journal-title":"Am. J. Physiol. Regul. Integr. Comp. Physiol."},{"key":"2026020208264063600_JEB251460C34","doi-asserted-by":"publisher","first-page":"2248","DOI":"10.1126\/science.1061967","article-title":"Effects of size and temperature on metabolic rate","volume":"293","author":"Gillooly","year":"2001","journal-title":"Science"},{"key":"2026020208264063600_JEB251460C35","doi-asserted-by":"publisher","first-page":"2961","DOI":"10.1242\/jeb.02319","article-title":"Cardiovascular and haematological responses of Atlantic cod (Gadus morhua) to acute temperature increase","volume":"209","author":"Gollock","year":"2006","journal-title":"J. Exp. Biol."},{"key":"2026020208264063600_JEB251460C36","doi-asserted-by":"publisher","first-page":"717","DOI":"10.1007\/s00360-020-01299-w","article-title":"The effect of temperature acclimation on the force-frequency relationship and adrenergic sensitivity of the ventricle of two populations of juvenile sockeye salmon","volume":"190","author":"Goulding","year":"2020","journal-title":"J. Comp. Physiol. B"},{"key":"2026020208264063600_JEB251460C37","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.225227","article-title":"Reduced ventricular excitability causes atrioventricular block and depression of heart rate in fish at critically high temperatures","volume":"223","author":"Haverinen","year":"2020","journal-title":"J. Exp. Biol."},{"key":"2026020208264063600_JEB251460C38","doi-asserted-by":"publisher","first-page":"R165","DOI":"10.1152\/ajpregu.00403.2016","article-title":"Maximum heart rate in brown trout (Salmo trutta fario) is not limited by firing rate of pacemaker cells","volume":"312","author":"Haverinen","year":"2017","journal-title":"Am. J. Physiol. Regul. Integr. Comp. Physiol."},{"key":"2026020208264063600_JEB251460C39","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1242\/jeb.167.1.47","article-title":"The influence of temperature on ryanodine sensitivity and the force-frequency relationship in the myocardium of rainbow trout","volume":"167","author":"Hove-Madsen","year":"1992","journal-title":"J. Exp. Biol."},{"key":"2026020208264063600_JEB251460C40","doi-asserted-by":"publisher","first-page":"R1902","DOI":"10.1152\/ajpregu.2001.281.6.R1902","article-title":"The function of the sarcoplasmic reticulum is not inhibited by low temperatures in trout atrial myocytes","volume":"281","author":"Hove-Madsen","year":"2001","journal-title":"Am. J. Physiol. Regul. Integr. Comp. Physiol."},{"key":"2026020208264063600_JEB251460C41","doi-asserted-by":"publisher","first-page":"evac173","DOI":"10.1093\/gbe\/evac173","article-title":"A revised perspective on the evolution of troponin I and troponin T gene families in vertebrates","volume":"15","author":"Joyce","year":"2023","journal-title":"Genome Biol. Evol."},{"key":"2026020208264063600_JEB251460C42","doi-asserted-by":"publisher","first-page":"287","DOI":"10.1016\/0300-9629(94)90097-3","article-title":"Maximum prolonged swimming speed and maximum cardiac performance of rainbow trout, Oncorhynchus mykiss, acclimated to two different water temperatures","volume":"108","author":"Keen","year":"1994","journal-title":"Comp. Biochem. Physiol. A Mol. Integr. Physiol."},{"key":"2026020208264063600_JEB251460C43","doi-asserted-by":"publisher","first-page":"201","DOI":"10.1016\/j.jtherbio.2012.01.002","article-title":"Blood oxygenation and cardiorespiratory function in steelhead trout (Oncorhynchus mykiss) challenged with an acute temperature increase and zatebradine-induced bradycardia","volume":"37","author":"Keen","year":"2012","journal-title":"J. Therm. Biol."},{"key":"2026020208264063600_JEB251460C44","doi-asserted-by":"publisher","first-page":"1211","DOI":"10.1139\/z92-168","article-title":"Cardiac physiology in tunas. II. Effect of ryanodine, calcium, and adrenaline on force-frequency relationships in atrial strips from skipjack tuna, Katsuwonus pelamis","volume":"70","author":"Keen","year":"1992","journal-title":"Can. J. Zool."},{"key":"2026020208264063600_JEB251460C45","doi-asserted-by":"publisher","first-page":"27","DOI":"10.1242\/jeb.181.1.27","article-title":"Thermal-acclimation alters both adrenergic sensitivity and adrenoceptor density in cardiac tissue of rainbow trout","volume":"181","author":"Keen","year":"1993","journal-title":"J. Exp. Biol."},{"key":"2026020208264063600_JEB251460C46","doi-asserted-by":"publisher","first-page":"438","DOI":"10.1007\/Bf00714580","article-title":"Effect of temperature and temperature acclimation on the ryanodine sensitivity of the trout myocardium","volume":"164","author":"Keen","year":"1994","journal-title":"J. Comp. Physiol. B Biochem. Syst. Env. Physiol."},{"key":"2026020208264063600_JEB251460C47","doi-asserted-by":"publisher","first-page":"1981","DOI":"10.1242\/jeb.052860","article-title":"The influence of trout cardiac troponin I and PKA phosphorylation on the Ca2+ affinity of the cardiac troponin complex","volume":"214","author":"Kirkpatrick","year":"2011","journal-title":"J. Exp. Biol."},{"key":"2026020208264063600_JEB251460C48","doi-asserted-by":"publisher","first-page":"1162","DOI":"10.1242\/jeb.065102","article-title":"Expression of SERCA and phospholamban in rainbow trout (Oncorhynchus mykiss) heart: comparison of atrial and ventricular tissue and effects of thermal acclimation","volume":"215","author":"Korajoki","year":"2012","journal-title":"J. Exp. Biol."},{"key":"2026020208264063600_JEB251460C49","doi-asserted-by":"publisher","first-page":"467","DOI":"10.1007\/s00360-012-0724-1","article-title":"Temperature dependence of sarco(endo)plasmic reticulum Ca2+-ATPase expression in fish hearts","volume":"183","author":"Korajoki","year":"2013","journal-title":"J. Comp. Physiol. B"},{"key":"2026020208264063600_JEB251460C50","doi-asserted-by":"publisher","first-page":"110564","DOI":"10.1016\/j.cbpa.2019.110564","article-title":"Contractile performance of the Alaska blackfish (Dallia pectoralis) ventricle: assessment of the effects of temperature, pacing frequency, the role of the sarcoplasmic reticulum in contraction and adrenergic stimulation","volume":"238","author":"Kubly","year":"2019","journal-title":"Comp. Biochem. Physiol. A Mol. Integr. Physiol."},{"key":"2026020208264063600_JEB251460C51","doi-asserted-by":"publisher","first-page":"R398","DOI":"10.1152\/ajpregu.00392.2003","article-title":"Temperature dependence of the Ca2+-ATPase (SERCA2) in the ventricles of tuna and mackerel","volume":"286","author":"Landeira-Fernandez","year":"2004","journal-title":"Am. J. Physiol. Regul. Integr. Comp. Physiol."},{"key":"2026020208264063600_JEB251460C52","doi-asserted-by":"publisher","first-page":"217","DOI":"10.1016\/j.jtherbio.2012.01.003","article-title":"Thermal dependence of cardiac SR Ca2+-ATPase from fish and mammals","volume":"37","author":"Landeira-Fernandez","year":"2012","journal-title":"J. Therm. Biol."},{"key":"2026020208264063600_JEB251460C53","doi-asserted-by":"publisher","first-page":"102","DOI":"10.1016\/0006-291X(90)91362-V","article-title":"The effects of pH and temperature on fluorescent calcium indicators as determined with Chelex-100 and EDTA buffer systems","volume":"171","author":"Lattanzio","year":"1990","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"2026020208264063600_JEB251460C54","first-page":"585A","article-title":"Low temperature reduces Na+\/Ca2+ exchange rate, but not SR Ca2+ release in trout atrial myocytes","volume":"80","author":"Llach","year":"2001","journal-title":"Biophys. J."},{"key":"2026020208264063600_JEB251460C55","doi-asserted-by":"publisher","first-page":"66","DOI":"10.1016\/j.cbpa.2012.05.183","article-title":"The temperature challenges on cardiac performance in winter-quiescent and migration-stage eels Anguilla anguilla","volume":"163","author":"Methling","year":"2012","journal-title":"Comp. Biochem. Physiol. A Mol. Integr. Physiol."},{"key":"2026020208264063600_JEB251460C56","doi-asserted-by":"publisher","first-page":"1701","DOI":"10.1139\/f67-140","article-title":"Catecholamine and carbohydrate concentrations in rainbow trout (Salmo gairdneri) in relation to physical disturbance","volume":"24","author":"Nakano","year":"1967","journal-title":"J. Fish. Res. Board Can."},{"key":"2026020208264063600_JEB251460C57","doi-asserted-by":"publisher","first-page":"655","DOI":"10.1242\/jeb.00791","article-title":"Effects of temperature and anoxia upon the performance of in situ perfused trout hearts","volume":"207","author":"Overgaard","year":"2004","journal-title":"J. Exp. Biol."},{"key":"2026020208264063600_JEB251460C58","doi-asserted-by":"publisher","first-page":"311","DOI":"10.1016\/j.cbpa.2011.04.020","article-title":"The interactive effects of a gradual temperature decrease and long-term food deprivation on cardiac and hepatic blood flows in rainbow trout (Oncorhynchus mykiss)","volume":"160","author":"Petersen","year":"2011","journal-title":"Comp. Biochem. Physiol. A Mol. Integr. Physiol."},{"key":"2026020208264063600_JEB251460C59","doi-asserted-by":"publisher","first-page":"713","DOI":"10.1007\/s00360-007-0166-3","article-title":"Importance of the sarcoplasmic reticulum and adrenergic stimulation on the cardiac contractility of the neotropical teleost Synbranchus marmoratus under different thermal conditions","volume":"177","author":"Rocha","year":"2007","journal-title":"J. Comp. Physiol. B"},{"key":"2026020208264063600_JEB251460C60","doi-asserted-by":"publisher","first-page":"1441","DOI":"10.1111\/jfb.14159","article-title":"The effect of acute warming and thermal acclimation on maximum heart rate of the common killifish Fundulus heteroclitus","volume":"95","author":"Safi","year":"2019","journal-title":"J. Fish Biol."},{"key":"2026020208264063600_JEB251460C61","doi-asserted-by":"publisher","first-page":"R2292","DOI":"10.1152\/ajpregu.00884.2006","article-title":"Venous hemodynamic responses to acute temperature increase in the rainbow trout (Oncorhynchus mykiss)","volume":"292","author":"Sandblom","year":"2007","journal-title":"Am. J. Physiol. Regul. Integr. Comp. Physiol."},{"key":"2026020208264063600_JEB251460C62","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1038\/nclimate2457","article-title":"Physiological plasticity increases resilience of ectothermic animals to climate change","volume":"5","author":"Seebacher","year":"2015","journal-title":"Nat. Clim. Change."},{"key":"2026020208264063600_JEB251460C63","doi-asserted-by":"publisher","first-page":"1607","DOI":"10.1242\/jeb.200.11.1607","article-title":"The effect of temperature and adrenaline on the relative importance of the sarcoplasmic reticulum in contributing Ca2+ to force development in isolated ventricular trabeculae from rainbow trout","volume":"200","author":"Shiels","year":"1997","journal-title":"J. Exp. Biol."},{"key":"2026020208264063600_JEB251460C64","doi-asserted-by":"publisher","first-page":"331","DOI":"10.1016\/S1095-6433(00)00161-6","article-title":"The effect of ryanodine on isometric tension development in isolated ventricular trabeculae from Pacific mackerel (Scomber japonicus)","volume":"125","author":"Shiels","year":"2000","journal-title":"Comp. Biochem. Physiol. A Mol. Integr. Physiol."},{"key":"2026020208264063600_JEB251460C65","doi-asserted-by":"publisher","first-page":"456","DOI":"10.1152\/physiol.00015.2014","article-title":"The sarcoplasmic reticulum and the evolution of the vertebrate heart","volume":"29","author":"Shiels","year":"2014","journal-title":"Physiol"},{"key":"2026020208264063600_JEB251460C66","doi-asserted-by":"publisher","first-page":"R1756","DOI":"10.1152\/ajpregu.00510.2004","article-title":"Temporal and spatial properties of cellular Ca2+ flux in trout ventricular myocytes","volume":"288","author":"Shiels","year":"2005","journal-title":"Am. J. Physiol. Regul. Integr. Comp. Physiol."},{"key":"2026020208264063600_JEB251460C67","doi-asserted-by":"publisher","first-page":"2701","DOI":"10.1242\/jeb.201.19.2701","article-title":"Effects of temperature, adrenaline and ryanodine on power production in rainbow trout Oncorhynchus mykiss ventricular trabeculae","volume":"201","author":"Shiels","year":"1998","journal-title":"J. Exp. Biol."},{"key":"2026020208264063600_JEB251460C68","doi-asserted-by":"publisher","first-page":"881","DOI":"10.1242\/jeb.202.7.881","article-title":"The sarcoplasmic reticulum plays a major role in isometric contraction in atrial muscle of yellowfin tuna","volume":"202","author":"Shiels","year":"1999","journal-title":"J. Exp. Biol."},{"key":"2026020208264063600_JEB251460C69","doi-asserted-by":"publisher","first-page":"2771","DOI":"10.1242\/jeb.203.18.2771","article-title":"Temperature-dependence of L-type Ca2+ channel current in atrial myocytes from rainbow trout","volume":"203","author":"Shiels","year":"2000","journal-title":"J. Exp. Biol."},{"key":"2026020208264063600_JEB251460C70","doi-asserted-by":"publisher","first-page":"3641","DOI":"10.1242\/jeb.205.23.3641","article-title":"Effects of temperature on intracellular [Ca2+] in trout atrial myocytes","volume":"205","author":"Shiels","year":"2002","journal-title":"J. Exp. Biol."},{"key":"2026020208264063600_JEB251460C71","doi-asserted-by":"publisher","first-page":"3631","DOI":"10.1242\/jeb.205.23.3631","article-title":"Temperature dependence of cardiac sarcoplasmic reticulum function in rainbow trout myocytes","volume":"205","author":"Shiels","year":"2002","journal-title":"J. Exp. Biol."},{"key":"2026020208264063600_JEB251460C72","doi-asserted-by":"publisher","first-page":"816","DOI":"10.1086\/378918","article-title":"Acute temperature change modulates the response of ICa to adrenergic stimulation in fish cardiomyocytes","volume":"76","author":"Shiels","year":"2003","journal-title":"Physiol. Biochem. Zool."},{"key":"2026020208264063600_JEB251460C73","doi-asserted-by":"publisher","first-page":"3091","DOI":"10.1242\/jeb.02321","article-title":"Sarcolemmal ion currents and sarcoplasmic reticulum Ca2+ content in ventricular myocytes from the cold stenothermic fish, the burbot (Lota lota)","volume":"209","author":"Shiels","year":"2006","journal-title":"J. Exp. Biol."},{"key":"2026020208264063600_JEB251460C74","doi-asserted-by":"publisher","first-page":"255","DOI":"10.1016\/B978-0-323-90801-6.00181-6","article-title":"Integrated responses of the heart to acute changes in temperature","volume-title":"Encyclopedia of Fish Physiology","author":"Shiels","year":"2024"},{"key":"2026020208264063600_JEB251460C75","doi-asserted-by":"publisher","first-page":"215","DOI":"10.1007\/s00300-008-0522-0","article-title":"The role of adrenaline as a modulator of cardiac performance in two Antarctic fishes","volume":"32","author":"Skov","year":"2009","journal-title":"Polar Biol."},{"key":"2026020208264063600_JEB251460C76","doi-asserted-by":"publisher","first-page":"615","DOI":"10.1038\/262615a0","article-title":"Phosphorylation of troponin I and the inotropic effect of adrenaline in the perfused rabbit heart","volume":"262","author":"Solaro","year":"1976","journal-title":"Nature"},{"key":"2026020208264063600_JEB251460C77","doi-asserted-by":"publisher","first-page":"3915","DOI":"10.1242\/jeb.019281","article-title":"The effect of acute temperature increases on the cardiorespiratory performance of resting and swimming sockeye salmon (Oncorhynchus nerka)","volume":"211","author":"Steinhausen","year":"2008","journal-title":"J. Exp. Biol."},{"key":"2026020208264063600_JEB251460C78","doi-asserted-by":"publisher","first-page":"coaf034","DOI":"10.1093\/conphys\/coaf034","article-title":"Climate warming will test the limits of thermal plasticity in rainbow trout, a globally distributed fish","volume":"13","author":"Strowbridge","year":"2025","journal-title":"Conserv. Physiol."},{"key":"2026020208264063600_JEB251460C79","doi-asserted-by":"publisher","first-page":"jeb215210","DOI":"10.1242\/jeb.215210","article-title":"A rapid intrinsic heart rate resetting response with thermal acclimation in rainbow trout, Oncorhynchus mykiss","volume":"223","author":"Sutcliffe","year":"2020","journal-title":"J. Exp. Biol."},{"key":"2026020208264063600_JEB251460C80","doi-asserted-by":"publisher","first-page":"141","DOI":"10.1111\/j.1095-8649.2001.tb02344.x","article-title":"Cold adaptation suppresses the contractility of both atrial and ventricular muscle of the crucian carp heart","volume":"59","author":"Tittu","year":"2001","journal-title":"J. Fish Biol."},{"key":"2026020208264063600_JEB251460C81","doi-asserted-by":"publisher","first-page":"1597","DOI":"10.1242\/jeb.205.11.1597","article-title":"Regulation of cardiac contractility in a cold stenothermal fish, the burbot Lota lota L","volume":"205","author":"Tiitu","year":"2002","journal-title":"J. Exp. Biol."},{"key":"2026020208264063600_JEB251460C82","author":"US Environmental Protection Agency","year":"2003"},{"key":"2026020208264063600_JEB251460C83","doi-asserted-by":"publisher","first-page":"477","DOI":"10.1016\/0742-8413(89)90101-1","article-title":"Regulation of contractility of the fish (Carassius carassius L.) heart ventricle","volume":"94","author":"Vornanen","year":"1989","journal-title":"Comp. Biochem. Physiol. C Comp. Pharmacol."},{"key":"2026020208264063600_JEB251460C84","doi-asserted-by":"publisher","first-page":"533","DOI":"10.1242\/jeb.201.4.533","article-title":"L-type Ca2+ current in fish cardiac myocytes: effects of thermal acclimation and beta-adrenergic stimulation","volume":"201","author":"Vornanen","year":"1998","journal-title":"J. Exp. Biol."},{"key":"2026020208264063600_JEB251460C85","doi-asserted-by":"publisher","first-page":"1941","DOI":"10.1242\/jeb.128439","article-title":"The temperature dependence of electrical excitability in fish hearts","volume":"219","author":"Vornanen","year":"2016","journal-title":"J. Exp. Biol."},{"key":"2026020208264063600_JEB251460C86","doi-asserted-by":"publisher","first-page":"99","DOI":"10.1016\/bs.fp.2017.04.002","article-title":"Electrical excitability of the fish heart and its autonomic regulation","volume-title":"Fish Physiology, Vol. 36A: The Cardiovascular System - Morphology, Control, and Function","author":"Vornanen","year":"2017"},{"key":"2026020208264063600_JEB251460C87","doi-asserted-by":"publisher","first-page":"43","DOI":"10.1007\/s00360-020-01313-1","article-title":"Effects of acute warming on cardiac and myotomal sarco(endo)plasmic reticulum ATPase (SERCA) of thermally acclimated brown trout (Salmo trutta)","volume":"191","author":"Vornanen","year":"2021","journal-title":"J. Comp. Physiol. B"},{"key":"2026020208264063600_JEB251460C88","doi-asserted-by":"publisher","first-page":"101","DOI":"10.1080\/19336950.2015.1121335","article-title":"Zebrafish heart as a model for human cardiac electrophysiology","volume":"10","author":"Vornanen","year":"2016","journal-title":"Channels"},{"key":"2026020208264063600_JEB251460C89","doi-asserted-by":"publisher","first-page":"827","DOI":"10.1016\/S1095-6433(02)00051-X","article-title":"Plasticity of excitation-contraction coupling in fish cardiac myocytes","volume":"132","author":"Vornanen","year":"2002","journal-title":"Comp. Biochem. Physiol. A Mol. Integr. Physiol."},{"key":"2026020208264063600_JEB251460C90","doi-asserted-by":"publisher","first-page":"299","DOI":"10.1242\/jeb.091272","article-title":"Acute heat tolerance of cardiac excitation in the brown trout (Salmo trutta fario)","volume":"217","author":"Vornanen","year":"2014","journal-title":"J. Exp. Biol."},{"key":"2026020208264063600_JEB251460C91","doi-asserted-by":"publisher","first-page":"jeb247446","DOI":"10.1242\/jeb.247446","article-title":"Cardiac arrhythmias in fish induced by natural and anthropogenic changes in environmental conditions","volume":"227","author":"Vornanen","year":"2024","journal-title":"J. Exp. Biol."},{"key":"2026020208264063600_JEB251460C92","doi-asserted-by":"publisher","first-page":"2440","DOI":"10.1139\/z79-316","article-title":"The influence of temperature and anaemia on the adrenergic and cholinergic mechanisms controlling heart rate in the rainbow trout","volume":"57","author":"Wood","year":"1979","journal-title":"Can. J. Zool."},{"key":"2026020208264063600_JEB251460C93","doi-asserted-by":"publisher","first-page":"H760","DOI":"10.1152\/ajpheart.1987.252.4.H760","article-title":"Intracellular [Ca2+] related to rate of force development in twitch contraction of heart","volume":"252","author":"Yue","year":"1987","journal-title":"Am. J. Physiol. Heart Circ. Physiol."}],"container-title":["Journal of Experimental Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.biologists.com\/jeb\/article-pdf\/doi\/10.1242\/jeb.251460\/3675282\/jeb251460.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/journals.biologists.com\/jeb\/article-pdf\/doi\/10.1242\/jeb.251460\/3683067\/jeb251460.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.biologists.com\/jeb\/article-pdf\/doi\/10.1242\/jeb.251460\/3683067\/jeb251460.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T13:26:50Z","timestamp":1770038810000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.biologists.com\/jeb\/article\/229\/2\/jeb251460\/370540\/Thermal-plasticity-of-adrenaline-mediated"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1,15]]},"references-count":93,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,1,15]]}},"URL":"https:\/\/doi.org\/10.1242\/jeb.251460","relation":{},"ISSN":["0022-0949","1477-9145"],"issn-type":[{"value":"0022-0949","type":"print"},{"value":"1477-9145","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1,15]]},"article-number":"jeb251460"}}