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However, storage conditions such as temperature and duration may bias these measurements. Here, we evaluated the stability of oxidative stress biomarkers, including three antioxidant enzymes (superoxide dismutase, glutathione reductase, glutathione peroxidase) and a lipid peroxidation marker (malondialdehyde) in amphibian, mammal, bird and insect samples stored under various temperature conditions (\u221280, \u221220, 4\u00b0C) from a few hours to 8\u00a0months. Storage significantly affected biomarker values depending on the marker, tissue and taxon. Notably, even long-term storage at \u221280\u00b0C altered some markers. In insect samples, lipid peroxidation was also influenced by triglyceride levels, indicating a potential confounding factor. Our results highlight the need to consider storage effects in oxidative stress studies. We also provide practical recommendations, aiming to improve data reliability across field and laboratory eco-evolutionary studies, as well as biomedical contexts.<\/jats:p>","DOI":"10.1242\/jeb.251748","type":"journal-article","created":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T10:12:29Z","timestamp":1770286349000},"source":"Crossref","is-referenced-by-count":3,"title":["Effects of storage conditions on oxidative stress biomarkers: methodological implications for ecological and evolutionary studies"],"prefix":"10.1242","volume":"229","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-0408-7421","authenticated-orcid":false,"given":"Francisco","family":"Miranda","sequence":"first","affiliation":[{"name":"Do\u00f1ana Biological Station 1 , Avenida Am\u00e9rico Vespucio 26, 41092 Sevilla ,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9515-9038","authenticated-orcid":false,"given":"Francisco","family":"Garcia-Gonzalez","sequence":"additional","affiliation":[{"name":"Do\u00f1ana Biological Station 1 , Avenida Am\u00e9rico Vespucio 26, 41092 Sevilla ,","place":["Spain"]},{"name":"Centre for Evolutionary Biology, School of Biological Sciences, University of Western Australia 2 , Crawley 6009, WA ,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marko","family":"Proki\u0107","sequence":"additional","affiliation":[{"name":"Institute for Biological Research \u201cSini\u0161a Stankovi\u0107\u201d \u2013 National Institute of Republic of Serbia, University of Belgrade 3 Department of Physiology , , Bulevar despota Stefana 142, 11108 Belgrade ,","place":["Serbia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miguel","family":"Lozano","sequence":"additional","affiliation":[{"name":"Do\u00f1ana Biological Station 1 , Avenida Am\u00e9rico Vespucio 26, 41092 Sevilla ,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8516-0999","authenticated-orcid":false,"given":"Antonio","family":"Carrillo-Vico","sequence":"additional","affiliation":[{"name":"Instituto de Biomedicina de Sevilla, IBiS\/Hospital Universitario Virgen del Roc\u00edo\/CSIC\/Universidad de Sevilla 4 , 41013 Seville ,","place":["Spain"]},{"name":"Universidad de Sevilla 5 Departamento de Bioqu\u00edmica M\u00e9dica y Biolog\u00eda Molecular e Inmunolog\u00eda, Facultad de Medicina , , 41009 Seville ,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eduardo","family":"Ponce-Espa\u00f1a","sequence":"additional","affiliation":[{"name":"Instituto de Biomedicina de Sevilla, IBiS\/Hospital Universitario Virgen del Roc\u00edo\/CSIC\/Universidad de Sevilla 4 , 41013 Seville ,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"\u00c1lvarez","sequence":"additional","affiliation":[{"name":"PROCAVI 6 Departamento de Seguridad Alimentaria , S.L. 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