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These experiments measure sarcomeric protein organization in healthy and diseased muscle and provide direct molecular evidence for these proteins\u2019 functional roles and dynamics. Many recent advances in sarcomeric protein research have relied on diffraction data; for example, structural and functional roles of cMyBP-C and titin, and conformational\/energetic states of actomyosin cross-bridges. LiX operations are optimized for high-throughput muscle diffraction with rapid turnover and semi-automated data processing. These operations have been tested on skeletal and cardiac tissues sourced from both humans and animal models including pig, rat, mouse and zebrafish. LiX's new capacity for muscle experimentation and support for new beamline users will accelerate the investigation of sarcomeric protein structure and function, muscle biomechanics and skeletal and cardiac myopathies.<\/jats:p>","DOI":"10.1242\/jeb.252250","type":"journal-article","created":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T07:13:32Z","timestamp":1785309212000},"source":"Crossref","is-referenced-by-count":0,"title":["Muscle diffraction at the Life Science X-ray Scattering beamline"],"prefix":"10.1242","volume":"229","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1581-6937","authenticated-orcid":false,"given":"Khoi D.","family":"Nguyen","sequence":"first","affiliation":[{"name":"Accelerated Muscle Biotechnologies 1 , Mansfield, MA 02048 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4278-4221","authenticated-orcid":false,"given":"Anthony L.","family":"Hessel","sequence":"additional","affiliation":[{"name":"Accelerated Muscle Biotechnologies 1 , Mansfield, MA 02048 ,","place":["USA"]},{"name":"Institute of Physiology II, University of Muenster 2 , Muenster 48149 ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1280-1484","authenticated-orcid":false,"given":"Rachel L.","family":"Sadler","sequence":"additional","affiliation":[{"name":"University of Arizona 3 Department of Physiology , , Tucson, AZ 85721 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0613-0925","authenticated-orcid":false,"given":"Nichlas M.","family":"Engels","sequence":"additional","affiliation":[{"name":"University of Arizona 4 Department of Cellular and Molecular Medicine , , Tucson, AZ 85721 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6824-9564","authenticated-orcid":false,"given":"Christine E.","family":"Delligatti","sequence":"additional","affiliation":[{"name":"Accelerated Muscle Biotechnologies 1 , Mansfield, MA 02048 ,","place":["USA"]},{"name":"Loyola University Chicago 5 Department of Cell and Molecular Physiology , , Chicago, IL 60657 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher T.","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Cardiovascular Innovation Research Center, Heart Vascular and Thoracic Institute, Cleveland Clinic 6 , Cleveland, OH 44196 ,","place":["USA"]},{"name":"Department of Chemical and Biomedical Engineering 7 , Cleveland State University, Cleveland, OH 44115 ,","place":["USA"]},{"name":"Department of Biomedical Engineering, Case Western Reserve University & Cleveland Clinic 8 , Cleveland, OH 44106 ,","place":["USA"]},{"name":"Diagnostic Radiology, Cleveland Clinic 9 , Cleveland, OH 44195 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephan","family":"Lange","sequence":"additional","affiliation":[{"name":"Department of Biomedicine, Aarhus University 10 , DK-8000 Aarhus C ,","place":["Denmark"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew A.","family":"Caporizzo","sequence":"additional","affiliation":[{"name":"Department of Molecular Physiology and Biophysics, University of Vermont 11 , Burlington, VT 05405 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0822-0238","authenticated-orcid":false,"given":"Samantha P.","family":"Harris","sequence":"additional","affiliation":[{"name":"University of Arizona 3 Department of Physiology , , Tucson, AZ 85721 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1057-9194","authenticated-orcid":false,"given":"Lin","family":"Yang","sequence":"additional","affiliation":[{"name":"National Synchrotron Light Source II, Brookhaven National Laboratory 12 , Upton, NY 11973-5000 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2026,9,17]]},"reference":[{"key":"2026092203215286600_JEB252250C1","doi-asserted-by":"publisher","first-page":"e70106","DOI":"10.1002\/jcsm.70106","article-title":"Fast myosin binding protein-c is a vital regulator in young and aged fast skeletal muscle homeostasis","volume":"16","author":"Baby","year":"2025","journal-title":"J. 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