{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:45:31Z","timestamp":1792788331860,"version":"4.5.0"},"reference-count":113,"publisher":"The Company of Biologists","issue":"20","funder":[{"name":"Natural Sciences and Engineering Research Council of Canada","award":["2020-04153"],"award-info":[{"award-number":["2020-04153"]}],"id":[{"id":"https:\/\/ror.org\/01h531d29","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,10,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>The field of aquatic toxicology has undergone a paradigm shift in recent decades. The movement away from observational data detailing the concentrations that produce deleterious effects and towards a mechanistic understanding of how toxicants affect organismal physiology has significant advantages for the field. This mechanistic approach allows for the prediction of toxicant effects across species and among toxicants with similar modes of action. Critically, knowledge of the pathways by which toxicants exert their impacts can provide insight into how environmental factors such as temperature, salinity, dissolved oxygen, pH, alkalinity, dissolved organic carbon, major ions and co-occurring toxicants interact to modify toxicity, providing a more nuanced understanding of toxicity in natural settings. This mechanism-based approach has driven changes in the regulatory environment, through the incorporation of predictive modelling tools that can determine, on a site-specific basis, safe thresholds for concentrations of toxicants in aquatic settings. This represents an exemplar of how fundamental experimental biology translates into real-world application. However, we argue that while mechanistic aquatic toxicology is an important subfield of experimental biology, there are a number of factors that make it unique. In this Commentary, we outline best practices for the experimental biologist conducting research into toxicant effects on aquatic biota, provide examples of the utility of mechanistic approaches to the field of aquatic toxicology and outline how future advances in aquatic toxicology could benefit from experimental biology approaches.<\/jats:p>","DOI":"10.1242\/jeb.252679","type":"journal-article","created":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T09:24:17Z","timestamp":1792056257000},"source":"Crossref","is-referenced-by-count":0,"title":["An aquatic toxicology primer for the experimental biologist and guidance for conducting robust stressor exposures"],"prefix":"10.1242","volume":"229","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6834-1571","authenticated-orcid":false,"given":"Tamzin A.","family":"Blewett","sequence":"first","affiliation":[{"name":"University of Alberta 1 Department of Biological Sciences , , Edmonton, AB , , T6G 2E9","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chris N.","family":"Glover","sequence":"additional","affiliation":[{"name":"University of Alberta 1 Department of Biological Sciences , , Edmonton, AB , , T6G 2E9","place":["Canada"]},{"name":"Faculty of Science and Technology and Athabasca River Basin Research Institute, Athabasca University 2 , Athabasca, AB , , T9S 3A3","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erin M.","family":"Leonard","sequence":"additional","affiliation":[{"name":"Wilfrid Laurier University 3 Department of Biology , , Waterloo, ON , , N2L 3C5","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Connor B.","family":"Stewart","sequence":"additional","affiliation":[{"name":"University of Alberta 1 Department of Biological Sciences , , Edmonton, AB , , T6G 2E9","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nic R.","family":"Bury","sequence":"additional","affiliation":[{"name":"School of Ocean and Earth Sciences, University of Southampton, National Oceanographic Centre 4 , Waterfront Campus, Southampton SO14 3ZH ,","place":["UK"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jenelle D.","family":"McCuaig","sequence":"additional","affiliation":[{"name":"University of Alberta 1 Department of Biological Sciences , , Edmonton, AB , , T6G 2E9","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James C.","family":"McGeer","sequence":"additional","affiliation":[{"name":"Wilfrid Laurier University 3 Department of Biology , , Waterloo, ON , , N2L 3C5","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chris M.","family":"Wood","sequence":"additional","affiliation":[{"name":"University of British Columbia 5 Department of Zoology , , 6270 University Blvd, Vancouver, BC , , V6T 1Z4","place":["Canada"]},{"name":"McMaster University 6 Department of Biology , , 1280 Main St. West, Hamilton, ON , , L8S 4K1","place":["Canada"]},{"name":"University of Miami Rosenstiel School of Marine, Atmospheric, and Earth Science 7 Department of Marine Biology and Ecology , , 4600 Rickenbacker Causeway, Miami, FL 33149-1031 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2026,10,15]]},"reference":[{"key":"2026101505235890000_JEB252679C1","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/S1532-0456(02)00113-8","article-title":"The biotic ligand model for metals: current research, future directions, regulatory implications: preface","volume":"133","author":"Adams","year":"2002","journal-title":"Comp. 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