{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T10:05:15Z","timestamp":1790244315443,"version":"4.1.0"},"reference-count":0,"publisher":"The Company of Biologists","license":[{"start":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T00:00:00Z","timestamp":1790208000000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"British Heart Foundation","award":["PG\/17\/77\/33125"],"award-info":[{"award-number":["PG\/17\/77\/33125"]}],"id":[{"id":"https:\/\/ror.org\/02wdwnk04","id-type":"ROR","asserted-by":"crossref"}]},{"name":"University of Manchester","award":["PhD Scholarship"],"award-info":[{"award-number":["PhD Scholarship"]}],"id":[{"id":"https:\/\/ror.org\/027m9bs27","id-type":"ROR","asserted-by":"crossref"}]},{"name":"British Heart Foundation","award":["PG\/17\/77\/33125"],"award-info":[{"award-number":["PG\/17\/77\/33125"]}],"id":[{"id":"https:\/\/ror.org\/02wdwnk04","id-type":"ROR","asserted-by":"crossref"}]},{"name":"University of Jyvaskyla","award":["Travelling Fellowship"],"award-info":[{"award-number":["Travelling Fellowship"]}],"id":[{"id":"https:\/\/ror.org\/05n3dz165","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>Phenanthrene is a polycyclic aromatic hydrocarbon (PAH) that is derived from fossil fuels and is present in most PAH mixtures found in water, air and soil. It has been shown to affect cardiac function of vertebrates, including fish. We investigated the effects of phenanthrene on intracellular calcium transient ([Ca2+]i) and the Na+\/Ca2+ exchanger (NCX) current (INCX) in zebrafish (Danio rerio) ventricular cardiomyocytes using Fluo-4 AM imaging and whole-cell patch-clamp, respectively. Phenanthrene exposure (25 \u00b5M) reduced the amplitude and slowed the rate of decay of Ca\u00b2\u207a transients. Phenanthrene also inhibited INCX with equal efficacy in forward- and reverse-mode. We conclude that phenanthrene disrupts Ca\u00b2\u207a handling in zebrafish ventricular myocytes, which could impact cardiac contractility.<\/jats:p>","DOI":"10.1242\/jeb.252945","type":"journal-article","created":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T09:13:03Z","timestamp":1790241183000},"source":"Crossref","is-referenced-by-count":0,"title":["Phenanthrene disrupts calcium transients and inhibits INCX in zebrafish (\n                    <i>Danio rerio<\/i>\n                    ) ventricular myocytes"],"prefix":"10.1242","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6061-9014","authenticated-orcid":false,"given":"Eeva-Riikka","family":"Vehni\u00e4inen","sequence":"first","affiliation":[{"name":"1 University of Jyv\u00e4skyl\u00e4, Department of Biological and Environmental Sciences, P.O. Box 35, FI-40014 University of Jyv\u00e4skyl\u00e4, Finland"},{"name":"2 University of Turku, Department of Biology, FI-20014 University of Turku, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7555-4452","authenticated-orcid":false,"given":"Charlotte R.","family":"Marris","sequence":"additional","affiliation":[{"name":"3 Faculty of Life Sciences, The University of Manchester, Core Technology Facility, Manchester, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5625-2491","authenticated-orcid":false,"given":"Shiva Nag","family":"Kompella","sequence":"additional","affiliation":[{"name":"3 Faculty of Life Sciences, The University of Manchester, Core Technology Facility, Manchester, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2055-6482","authenticated-orcid":false,"given":"Jules C.","family":"Hancox","sequence":"additional","affiliation":[{"name":"4 Bristol Medical School, Translational Health Sciences, University of Bristol, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5223-5205","authenticated-orcid":false,"given":"Holly A.","family":"Shiels","sequence":"additional","affiliation":[{"name":"3 Faculty of Life Sciences, The University of Manchester, Core Technology Facility, Manchester, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2026,9,24]]},"container-title":["Journal of Experimental Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.biologists.com\/jeb\/article-pdf\/doi\/10.1242\/jeb.252945\/3719135\/jeb252945.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/journals.biologists.com\/jeb\/article-pdf\/doi\/10.1242\/jeb.252945\/3719135\/jeb252945.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T09:13:04Z","timestamp":1790241184000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.biologists.com\/jeb\/article\/doi\/10.1242\/jeb.252945\/373003\/Phenanthrene-disrupts-calcium-transients-and"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,24]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1242\/jeb.252945","relation":{},"ISSN":["0022-0949","1477-9145"],"issn-type":[{"value":"0022-0949","type":"print"},{"value":"1477-9145","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,24]]},"article-number":"jeb.252945"}}