{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T14:14:32Z","timestamp":1772806472560,"version":"3.50.1"},"reference-count":30,"publisher":"The Company of Biologists","issue":"2","license":[{"start":{"date-parts":[[1991,2,1]],"date-time":"1991-02-01T00:00:00Z","timestamp":665366400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1991,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The Abdominal-B (Abd-B) gene, a member of the Drosophila bithorax complex, is required during development to specify the identity of parasegments 10\u2013 14. Based on genetic studies, Casanova, J., S\u00e1nchez-Herrero, E. and Morata, G. (1986) Cell 47, 627-636, proposed that the Abd-B gene consists of two distinct elements that provide a morphogenetic (m) function in PS 10-13 and a regulatory (r) function in PS 14, where it represses m function. Here we present molecular confirmation of this genetic model. . Using specific antibodies, we show that the 55x103Mr and 30x103MrAbd-B proteins, predicted by cDNA analysis, are indeed present in PS 10-13 and PS 14, respectively. We also examine Abd-B mRNA and protein expression patterns in embryos mutant for either the m or r function. These data allow us to unambiguously assign m function to the 55x103Mr protein and r function to the 30x103Mr protein. Furthermore, as postulated by the model, transcription of the mRNA encoding the m protein is derepressed in PS 14 in the absence of r function. We have also studied the effect of mutations mapping in the infra-abdominal (iab) region located downstream of the Abd-B gene. Genetic studies suggest that the iab region contains crv-acting regulatory elements controlling Abd-B expression in PS 10-12. We present molecular evidence for the presence of downstream crs-regulatory elements by analyzing Abd-B mRNA and protein patterns in iab-6 and iab-7 embryos. Our analysis reveals the presence of parasegment and cell-specific regulatory elements of the Abd-B gene within each iab region. The Abd-B gene may provide a model for the understanding of similarly complex homeotic genes in higher organisms.<\/jats:p>","DOI":"10.1242\/dev.111.2.393","type":"journal-article","created":{"date-parts":[[2021,4,26]],"date-time":"2021-04-26T01:38:03Z","timestamp":1619401083000},"page":"393-405","source":"Crossref","is-referenced-by-count":77,"title":["Molecular definition of the morphogenetic and regulatory functions and the <i>cis<\/i>-regulatory elements of the <i>Drosophila Abd-B<\/i> homeotic gene"],"prefix":"10.1242","volume":"111","author":[{"given":"Anne M.","family":"Boulet","sequence":"first","affiliation":[{"name":"University of Utah School of Medicine Howard Hughes Medical Institute , Department of Human Genetics , , Salt Lake City, Utah 84132, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alan","family":"Lloyd","sequence":"additional","affiliation":[{"name":"University of Utah School of Medicine Howard Hughes Medical Institute , Department of Human 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