{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T03:56:53Z","timestamp":1773287813326,"version":"3.50.1"},"reference-count":33,"publisher":"The Company of Biologists","issue":"4","license":[{"start":{"date-parts":[[1994,4,1]],"date-time":"1994-04-01T00:00:00Z","timestamp":765158400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1994,4,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>E-cadherin function was disrupted in vivo in developing Xenopus laevis embryos through the expression of a mutant E-cadherin protein lacking its cytoplasmic tail. This truncated form of E-cadherin was designed to act as a dominant negative mutant by competing with the extracellular interactions of wild-type endogenous E-cadherin. Expression of truncated E-cadherin in the early embryo causes lesions to develop in the ectoderm during gastrulation. In contrast, expression of a similarly truncated N-cadherin protein failed to cause the lesions. The ectodermal defect caused by the truncated E-cadherin is rescued by overexpression of wild-type E-cadherin, by co-injection of full-length E-cadherin RNA along with the RNA for the truncated form. Overexpression of full-length C-cadherin, however, is unable to compensate for the disruption of E-cadherin function and can actually cause similar ectodermal lesions when injected alone, suggesting that there is a specific requirement for E-cadherin. Therefore, E-cadherin seems to be specifically required for maintaining the integrity of the ectoderm during epiboly in the gastrulating Xenopus embryo. Differential cadherin expression reflects, therefore, the requirement for distinct adhesive properties during different morphogenetic cell behaviors.<\/jats:p>","DOI":"10.1242\/dev.120.4.901","type":"journal-article","created":{"date-parts":[[2021,4,23]],"date-time":"2021-04-23T18:50:21Z","timestamp":1619203821000},"page":"901-909","source":"Crossref","is-referenced-by-count":143,"title":["Selective disruption of E-cadherin function in early <i>Xenopus<\/i> embryos by a dominant negative mutant"],"prefix":"10.1242","volume":"120","author":[{"given":"Elena","family":"Levine","sequence":"first","affiliation":[{"name":"University of California San Francisco 1 Department of Biochemistry , , San Francisco, CA 94143-0448, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chung Hyun","family":"Lee","sequence":"additional","affiliation":[{"name":"2 Cellular Biochemistry and Biophysics Program, Memorial Sloan-Kettering Cancer Center, 1275 York Ave., New York, NY 10021, USA"},{"name":"University of California San Francisco 4 Department of Pharmacology , , San Francisco, CA 94143-0450, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chris","family":"Kintner","sequence":"additional","affiliation":[{"name":"3 Molecular Neurobiology Laboratory, The Salk Institute for Biological Studies, San Diego, CA 92138, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barry M.","family":"Gumbiner","sequence":"additional","affiliation":[{"name":"2 Cellular Biochemistry and Biophysics Program, Memorial Sloan-Kettering Cancer Center, 1275 York Ave., New York, NY 10021, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[1994,4,1]]},"reference":[{"key":"2024121805393993700_DEVELOP_120_4_901C1","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/0092-8674(91)90616-7","article-title":"Expression of a dominant negative mutant of the FGF receptor disrupts mesoderm formation in Xenopus embryos","volume":"66","author":"Amaya","year":"1991","journal-title":"Cell"},{"key":"2024121805393993700_DEVELOP_120_4_901C2","doi-asserted-by":"crossref","first-page":"829","DOI":"10.1242\/dev.111.3.829","article-title":"Differential expression of two cadherins in Xenopus laevis","volume":"111","author":"Angres","year":"1991","journal-title":"Development"},{"key":"2024121805393993700_DEVELOP_120_4_901C3","doi-asserted-by":"crossref","first-page":"1253","DOI":"10.1083\/jcb.110.4.1253","article-title":"Purified N-cadherin is a potent substrate for the rapid induction of neurite outgrowth","volume":"110","author":"Bixby","year":"1990","journal-title":"J. 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