{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,8]],"date-time":"2026-02-08T03:14:41Z","timestamp":1770520481062,"version":"3.49.0"},"reference-count":37,"publisher":"The Company of Biologists","issue":"2","license":[{"start":{"date-parts":[[1973,3,1]],"date-time":"1973-03-01T00:00:00Z","timestamp":99792000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1973,3,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>The release of wall-held materials from the pollen of ragweed (Ambrosia tenuifolia) and Cosmos bipimuxtus on to the stigma surface has been followed. When fresh stigmas were viewed by scanning electron microscopy, a fluid material was observed coating pollen grains, pollen tubes and adjacent stigmatic papillae. This fluid contained pollen-wall antigens, including the allergen Antigen E, detected by immunofluorescence, and proteins, lipids and carbohydrates detected by cytochemical methods.<\/jats:p>\n               <jats:p>In Cosmos, the fate of the antigens was much the same after both compatible and incompatible matings. In incompatible matings, pollen tubes were blocked with a polysaccharide considered to be callose. Callose particles appeared on neighbouring stigmatic papillae, and adjacent stigmatic hairs accumulated callose internally. This reaction may be an important one for the incompatibility response.<\/jats:p>","DOI":"10.1242\/jcs.12.2.421","type":"journal-article","created":{"date-parts":[[2021,4,24]],"date-time":"2021-04-24T03:14:39Z","timestamp":1619234079000},"page":"421-443","source":"Crossref","is-referenced-by-count":64,"title":["Pollen wall proteins: Pollen-Stigma interactions in ragweed and <i>Cosmos<\/i> (compositae)"],"prefix":"10.1242","volume":"12","author":[{"given":"R. B.","family":"Knox","sequence":"first","affiliation":[{"name":"Australian National University Botany Department , , P.O. Box 4, Canberra A.C.T., Australia"}]}],"member":"237","published-online":{"date-parts":[[1973,3,1]]},"reference":[{"key":"2023062012161527700_JOCES_12_2_421C1","doi-asserted-by":"publisher","first-page":"754","DOI":"10.1159\/000230460","article-title":"Demonstration of birch pollen allergen from isolated pollen grains using immunofluorescence and a single radical immunodiffusion technique","volume":"40","author":"Belin","year":"1971","journal-title":"Int. Arch. 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