{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,9]],"date-time":"2025-11-09T07:48:20Z","timestamp":1762674500207},"reference-count":32,"publisher":"The Company of Biologists","issue":"1","license":[{"start":{"date-parts":[[1978,6,1]],"date-time":"1978-06-01T00:00:00Z","timestamp":265507200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1978,6,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Following extraction of actomyosin and tubulin from cultured cells treated with Triton X-100, a cytoskeleton remains which is composed predominantly of the cell nucleus encompassed by a network of 10-nm filaments. After negative staining the dense perinuclear region appears as a densely woven filament net punctuated by patches of high electron density. Enucleation of 3T3cells with cytochalasin B gives rise to karyoplasts surrounded by 10-nm filaments and cytoplasts in which 10-nm filaments remain situated in the central region of the cytoplasm. While the 10-nm filaments occurred mainly as single filaments in human skin fibroblasts and 3T3cells, in epithelioid PtKi and PtK2 cells they were commonly associated in prominent meandering bundles. In addition, in these latter cells after Triton extraction the remaining ribosomes were bound specifically to the 10-nm-filament net.<\/jats:p>\n               <jats:p>After exposure of 3T3cells to cytochalasin B the 10-nm filaments formed branches that radiated from the perinuclear region into the immobile cell extensions. Concavalin A had no marked effect on the distribution of the 10-nm-filament net.<\/jats:p>\n               <jats:p>The results suggest that the 10-nm filaments act primarily as structural elements, serving, in particular, to support and constrain the nucleus in its position in the cell.<\/jats:p>","DOI":"10.1242\/jcs.31.1.393","type":"journal-article","created":{"date-parts":[[2021,4,26]],"date-time":"2021-04-26T20:47:51Z","timestamp":1619470071000},"page":"393-409","source":"Crossref","is-referenced-by-count":78,"title":["Direct visualization of the 10-nm (100- \u00c5)-filament network in whole and enucleated cultured cells"],"prefix":"10.1242","volume":"31","author":[{"given":"J. V.","family":"Small","sequence":"first","affiliation":[{"name":"* Institute of Molecular Biology of the Austrian Academy of Sciences, Billrothstrasse 11, 5020 Salzburg, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. E.","family":"Celist","sequence":"additional","affiliation":[{"name":"\u2020 Division of Biostructural Chemistry, Institute of Chemistry, Aarhus University, 8000 Aarhus C, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[1978,6,1]]},"reference":[{"key":"2023053021141723300_JOCES_31_1_393C1","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1083\/jcb.36.1.111","article-title":"The effect of mitotic inhibitors on myogenesis in vitro","volume":"36","author":"Bischoff","year":"1968","journal-title":"J. Cell Biol"},{"key":"2023053021141723300_JOCES_31_1_393C2","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1083\/jcb.70.2.459","article-title":"Rings of intermediate (100\u00c5) filament bundles in the perinuclear region of vascular endothelial cells: Their mobilization by colcemid and mitosis","volume":"70","author":"Blose","year":"1976","journal-title":"J. Cell Biol"},{"key":"2023053021141723300_JOCES_31_1_393C3","doi-asserted-by":"crossref","first-page":"662","DOI":"10.1073\/pnas.74.2.662","article-title":"Localization of bovine brain filament antibody on intermediate (100\u00c5) filaments in guinea pig vascular cells and chick cardiac muscle cells","volume":"74","author":"Blose","year":"1977","journal-title":"Proc. natn. Acad. Sci. U.S.A"},{"key":"2023053021141723300_JOCES_31_1_393C4","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1016\/0014-4827(75)90261-X","article-title":"The occurrence and possible role of 80-100A filaments in PtK? cells","volume":"68","author":"Brecher","year":"1975","journal-title":"Expl Cell Res"},{"key":"2023053021141723300_JOCES_31_1_393C5","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1083\/jcb.68.3.539","article-title":"A filamentous cytoskeleton in vertebrate smooth muscle fibres","volume":"68","author":"Cooke","year":"1976","journal-title":"J. Cell Biol"},{"key":"2023053021141723300_JOCES_31_1_393C6","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1083\/jcb.65.2.271","article-title":"Response of myogenic and fibrogenic cells to cytochalasin B and to colcemid","volume":"65","author":"Croop","year":"1975","journal-title":"J. Cell Biol"},{"key":"2023053021141723300_JOCES_31_1_393C7","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1083\/jcb.65.1.123","article-title":"Concanavalin A receptors, immunoglobulins and \u03b8 antigen of the lymphocyte surface","volume":"65","author":"De Petris","year":"1975","journal-title":"J. Cell Biol"},{"key":"2023053021141723300_JOCES_31_1_393C8","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1126\/science.769162","article-title":"Surface modulation in cell recognition and cell growth. Some new hypotheses on phenotype alterations and transmembranous control of cell receptors","volume":"192","author":"Edelman","year":"1976","journal-title":"Science, N.Y"},{"key":"2023053021141723300_JOCES_31_1_393C9","first-page":"411","article-title":"Ultrastructural and biochemical observations on cytoplasmic filaments of heart purkinje fibres","volume":"54","author":"Eriksson","year":"1976","journal-title":"J. Ultrastruct. Res"},{"key":"2023053021141723300_JOCES_31_1_393C10","volume-title":"An Adas of Fine Structure: The Cell, Its Organelles and Inclusions","author":"Fawcett","year":"1966"},{"key":"2023053021141723300_JOCES_31_1_393C11","doi-asserted-by":"crossref","first-page":"604","DOI":"10.1038\/261604a0","article-title":"Different distribution of 100A filaments in resting and locomotive leukaemic cells","volume":"261","author":"Felix","year":"1976","journal-title":"Nature, Lond"},{"key":"2023053021141723300_JOCES_31_1_393C12","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1101\/SQB.1973.037.01.063","article-title":"Functions of cytoplasmic fibres in non-muscle cell motility","volume":"37","author":"Goldman","year":"1973","journal-title":"Cold Spring Harb. Symp. quant. Biol"},{"key":"2023053021141723300_JOCES_31_1_393C13","doi-asserted-by":"crossref","first-page":"750","DOI":"10.1073\/pnas.70.3.750","article-title":"Preservation of normal behaviour by enucleated cells in culture","volume":"70","author":"Goldman","year":"1973","journal-title":"Proc. natn. Acad. Sci. U.S.A"},{"key":"2023053021141723300_JOCES_31_1_393C14","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1083\/jcb.60.2.346","article-title":"The effects of parathyroid hormone, colchicine and calcitonin on the ultrastructure and the activity of osteoclasts in organ culture","volume":"60","author":"Holltrop","year":"1974","journal-title":"J. Cell Biol"},{"key":"2023053021141723300_JOCES_31_1_393C15","first-page":"37","article-title":"Arrowhead complexes in a variety of cell types","volume-title":"In Exploratory Concepts in Muscular Dystrophy","author":"Ishikawa","year":"1974"},{"key":"2023053021141723300_JOCES_31_1_393C16","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1038\/268240a0","article-title":"Human smooth muscle autoantibodies reacting with intermediate (100\u00c5) filaments","volume":"268","author":"Kurki","year":"1977","journal-title":"Nature, Lond"},{"key":"2023053021141723300_JOCES_31_1_393C17","doi-asserted-by":"crossref","first-page":"4344","DOI":"10.1073\/pnas.73.12.4344","article-title":"Immunological characterisation of the 100 A filaments from muscle cells","volume":"73","author":"Lazarides","year":"1976","journal-title":"Proc. natn. Acad. Sci. U.S.A"},{"key":"2023053021141723300_JOCES_31_1_393C18","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1038\/264272a0","article-title":"Cell surface design","volume":"264","author":"Loor","year":"1976","journal-title":"Nature, Lond"},{"key":"2023053021141723300_JOCES_31_1_393C19","first-page":"1","article-title":"Trans-membrane control of the receptors of normal and tumour cells. II. Surface changes associated with transformation and malignancy","volume":"458","author":"Nicolson","year":"1976","journal-title":"Biochim. biophys. Acta"},{"key":"2023053021141723300_JOCES_31_1_393C20","doi-asserted-by":"crossref","first-page":"2490","DOI":"10.1073\/pnas.74.6.2490","article-title":"Visualization of a system of filaments 7-10 nm thick in cultured cells of an epithelioid line (PtK2) by immunofluorescence microscopy","volume":"74","author":"Osborn","year":"1977","journal-title":"Proc. natn. Acad. Sci. U.S.A"},{"key":"2023053021141723300_JOCES_31_1_393C21","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1016\/0014-4827(72)90322-9","article-title":"Enucleation of mammalian cells with cytochalasin B","volume":"71","author":"Prescott","year":"1972","journal-title":"Expl Cell Res"},{"key":"2023053021141723300_JOCES_31_1_393C22","doi-asserted-by":"crossref","first-page":"1625","DOI":"10.1073\/pnas.70.6.1625","article-title":"A comparative evaluation of the distribution of concanavalin-A binding sites on the surfaces of normal, virally-transformed and protease-treated fibroblasts","volume":"70","author":"Rosenblith","year":"1973","journal-title":"Proc. natn. Acad. Sci. U.S.A"},{"key":"2023053021141723300_JOCES_31_1_393C23","article-title":"Filament arrangements in negatively-stained cultured cells: the organization of actin","volume-title":"Cytobiologie (in Press)","author":"Small","year":"1977"},{"key":"2023053021141723300_JOCES_31_1_393C24","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1242\/jcs.23.1.243","article-title":"Studies on the formation and composition of the 10-nm (100-A) filaments of vertebrate smooth muscle","volume":"23","author":"Small","year":"1977","journal-title":"J. Cell Sci"},{"key":"2023053021141723300_JOCES_31_1_393C25","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/0022-2836(72)90390-7","article-title":"Structural basis of contraction in vertebrate smooth muscle","volume":"67","author":"Small","year":"1972","journal-title":"J. molec. Biol"},{"key":"2023053021141723300_JOCES_31_1_393C26","doi-asserted-by":"crossref","first-page":"434","DOI":"10.1016\/0012-1606(72)90183-2","article-title":"Mapping of concanavalin A binding sites on the surface of several cell types","volume":"27","author":"Smith","year":"1972","journal-title":"Devi Biol"},{"key":"2023053021141723300_JOCES_31_1_393C27","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/0022-2836(76)90074-7","article-title":"Myosin-linked calcium regulation in vertebrate smooth muscle","volume":"101","author":"Sobieszek","year":"1976","journal-title":"J. molec. Biol"},{"key":"2023053021141723300_JOCES_31_1_393C28","doi-asserted-by":"crossref","first-page":"2422","DOI":"10.1073\/pnas.74.6.2422","article-title":"Isolation and preliminary characterization of 10-nm filaments from baby hamster kidney (BHK-21) cells","volume":"74","author":"Starger","year":"1977","journal-title":"Proc. natn. Acad. Sci. U.S.A"},{"key":"2023053021141723300_JOCES_31_1_393C29","doi-asserted-by":"crossref","first-page":"484","DOI":"10.1083\/jcb.50.2.484","article-title":"Cytoplasmic filaments in developing and adult vertebrate smooth muscle","volume":"50","author":"Uehara","year":"1971","journal-title":"J. Cell Biol"},{"key":"2023053021141723300_JOCES_31_1_393C30","doi-asserted-by":"crossref","first-page":"4085","DOI":"10.1073\/pnas.73.11.4085","article-title":"Active cell edge and movements of concanavalin A receptors of the surface of epithelial and fibroblastic cells","volume":"73","author":"Vasiliev","year":"1976","journal-title":"Proc. natn. Acad. Sci. U.S.A"},{"key":"2023053021141723300_JOCES_31_1_393C31","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0040-8166(70)80002-7","article-title":"Neurofilaments and glial filaments","volume":"2","author":"Wuerker","year":"1970","journal-title":"Tissue & Cell"},{"key":"2023053021141723300_JOCES_31_1_393C32","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1073\/pnas.73.2.529","article-title":"Biochemistry of the filaments of brain","volume":"73","author":"Yen","year":"1976","journal-title":"Proc. natn. Acad. Sci. U.S.A"}],"container-title":["Journal of Cell Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.biologists.com\/jcs\/article-pdf\/31\/1\/393\/2887678\/joces_31_1_393.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.biologists.com\/jcs\/article-pdf\/31\/1\/393\/2887678\/joces_31_1_393.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,30]],"date-time":"2023-05-30T21:14:41Z","timestamp":1685481281000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.biologists.com\/jcs\/article\/31\/1\/393\/58593\/Direct-visualization-of-the-10-nm-100-A-filament"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1978,6,1]]},"references-count":32,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1978,6,1]]}},"URL":"https:\/\/doi.org\/10.1242\/jcs.31.1.393","relation":{},"ISSN":["0021-9533","1477-9137"],"issn-type":[{"value":"0021-9533","type":"print"},{"value":"1477-9137","type":"electronic"}],"subject":[],"published":{"date-parts":[[1978,6,1]]}}}