{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T14:07:39Z","timestamp":1761919659866},"reference-count":65,"publisher":"The Company of Biologists","issue":"3","license":[{"start":{"date-parts":[[2020,9,3]],"date-time":"2020-09-03T00:00:00Z","timestamp":1599091200000},"content-version":"vor","delay-in-days":2786,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-sa\/3.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,3,15]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>In animal cells the centrosome is commonly viewed as the main cellular structure driving microtubule (MT) assembly into the mitotic spindle apparatus. However, additional pathways, such as those mediated by chromatin and augmin, are involved in the establishment of functional spindles. The molecular mechanisms involved in these pathways remain poorly understood, mostly due to limitations inherent to current experimental systems available. To overcome these limitations we have developed six new Drosophila cell lines derived from Drosophila homozygous mutants for DSas-4, a protein essential for centriole biogenesis. These cells lack detectable centrosomal structures, astral MT, with dispersed pericentriolar proteins D-PLP, Centrosomin and \u03b3-tubulin. They show poorly focused spindle poles that reach the plasma membrane. Despite being compromised for functional centrosome, these cells could successfully undergo mitosis.<\/jats:p><jats:p>Live-cell imaging analysis of acentriolar spindle assembly revealed that nascent MTs are nucleated from multiple points in the vicinity of chromosomes. These nascent MTs then grow away from kinetochores allowing the expansion of fibers that will be part of the future acentriolar spindle. MT repolymerization assays illustrate that acentriolar spindle assembly occurs \u201cinside-out\u201d from the chromosomes. Colchicine-mediated depolymerization of MTs further revealed the presence of a functional Spindle Assembly Checkpoint (SAC) in the acentriolar cells. Finally, pilot RNAi experiments open the potential use of these cell lines for the molecular dissection of anastral pathways in spindle and centrosome assembly.<\/jats:p>","DOI":"10.1242\/bio.20133327","type":"journal-article","created":{"date-parts":[[2013,1,19]],"date-time":"2013-01-19T05:22:50Z","timestamp":1358572970000},"page":"314-323","source":"Crossref","is-referenced-by-count":23,"title":["Establishment and mitotic characterization of new<i>Drosophila<\/i>acentriolar cell lines from<i>DSas-4<\/i>mutant"],"prefix":"10.1242","volume":"2","author":[{"given":"Nicolas","family":"Lecland","sequence":"first","affiliation":[{"name":"Polarity and Morphogenesis Group, Jacques Monod Institute, UMR 7592 CNRS, University Paris Diderot, 15 rue H\u00e9l\u00e8ne Brion, 75 205 Paris Cedex 13, France"},{"name":"Present address: Microtubule Organization Lab, Institut de Recerca Biom\u00e8dica de Barcelona, Baldiri Reixac 10\u201312, 08028 Barcelona, Spain"}]},{"given":"Alain","family":"Debec","sequence":"additional","affiliation":[{"name":"Polarity and Morphogenesis Group, Jacques Monod Institute, UMR 7592 CNRS, University Paris Diderot, 15 rue H\u00e9l\u00e8ne Brion, 75 205 Paris Cedex 13, France"}]},{"given":"Audrey","family":"Delmas","sequence":"additional","affiliation":[{"name":"Centre de Biologie du D\u00e9veloppement, Universit\u00e9 Paul Sabatier, B\u00e2timent 4R3, 118 Route de Narbonne, 31062 Toulouse Cedex 9, France"}]},{"given":"Sara","family":"Moutinho-Pereira","sequence":"additional","affiliation":[{"name":"Chromosome Instability and Dynamics Laboratory, Instituto de Biologia Molecular e Celular, Universidade do Porto, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal"}]},{"given":"Nicolas","family":"Malmanche","sequence":"additional","affiliation":[{"name":"Chromosome Instability and Dynamics Laboratory, Instituto de Biologia Molecular e Celular, Universidade do Porto, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal"}]},{"given":"Anais","family":"Bouissou","sequence":"additional","affiliation":[{"name":"Centre de Biologie du D\u00e9veloppement, Universit\u00e9 Paul Sabatier, B\u00e2timent 4R3, 118 Route de Narbonne, 31062 Toulouse Cedex 9, France"}]},{"given":"Cl\u00e9mence","family":"Dupr\u00e9","sequence":"additional","affiliation":[{"name":"Polarity and Morphogenesis Group, Jacques Monod Institute, UMR 7592 CNRS, University Paris Diderot, 15 rue H\u00e9l\u00e8ne Brion, 75 205 Paris Cedex 13, France"}]},{"given":"Aimie","family":"Jourdan","sequence":"additional","affiliation":[{"name":"Polarity and Morphogenesis Group, Jacques Monod Institute, UMR 7592 CNRS, University Paris Diderot, 15 rue H\u00e9l\u00e8ne Brion, 75 205 Paris Cedex 13, France"}]},{"given":"Brigitte","family":"Raynaud-Messina","sequence":"additional","affiliation":[{"name":"Centre de Biologie du D\u00e9veloppement, Universit\u00e9 Paul Sabatier, B\u00e2timent 4R3, 118 Route de Narbonne, 31062 Toulouse Cedex 9, France"}]},{"given":"Helder","family":"Maiato","sequence":"additional","affiliation":[{"name":"Centre de Biologie du D\u00e9veloppement, Universit\u00e9 Paul Sabatier, B\u00e2timent 4R3, 118 Route de Narbonne, 31062 Toulouse Cedex 9, France"},{"name":"Chromosome Instability and Dynamics Laboratory, Instituto de Biologia Molecular e Celular, Universidade do Porto, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal"},{"name":"Department of Experimental Biology, Faculdade de Medicina, Universidade do Porto, 4200-319 Porto, Portugal"}]},{"given":"Antoine","family":"Guichet","sequence":"additional","affiliation":[{"name":"Polarity and Morphogenesis Group, Jacques Monod Institute, UMR 7592 CNRS, University Paris Diderot, 15 rue H\u00e9l\u00e8ne Brion, 75 205 Paris Cedex 13, France"}]}],"member":"237","published-online":{"date-parts":[[2013,1,17]]},"reference":[{"key":"2021080923324857100_b1","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1002\/3527603808.ch6","article-title":"The centrosome in evolution.","volume-title":"Centrosomes In Development And Disease","author":"Azimzadeh","year":"2004"},{"key":"2021080923324857100_b2","doi-asserted-by":"publisher","first-page":"R816","DOI":"10.1016\/j.cub.2010.08.010","article-title":"Building the centriole.","volume":"20","author":"Azimzadeh","year":"2010","journal-title":"Curr. 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