{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T18:46:28Z","timestamp":1791225988697,"version":"4.1.0"},"reference-count":49,"publisher":"Pharmaceutical Society of Japan","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biological &amp; Pharmaceutical Bulletin","Biol. Pharm. Bull."],"published-print":{"date-parts":[[2014]]},"DOI":"10.1248\/bpb.b13-00768","type":"journal-article","created":{"date-parts":[[2014,3,31]],"date-time":"2014-03-31T23:20:19Z","timestamp":1396308019000},"page":"552-559","source":"Crossref","is-referenced-by-count":20,"title":["Ternary Complex of Plasmid DNA Electrostatically Assembled with Polyamidoamine Dendrimer and Chondroitin Sulfate for Effective and Secure Gene Delivery"],"prefix":"10.1248","volume":"37","author":[{"given":"Masanobu","family":"Imamura","sequence":"first","affiliation":[{"name":"Department of Hospital Pharmacy, Nagasaki University Hospital"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yukinobu","family":"Kodama","sequence":"additional","affiliation":[{"name":"Department of Hospital Pharmacy, Nagasaki University Hospital"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norihide","family":"Higuchi","sequence":"additional","affiliation":[{"name":"Department of Hospital Pharmacy, Nagasaki University Hospital"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kosuke","family":"Kanda","sequence":"additional","affiliation":[{"name":"Department of Hospital Pharmacy, Nagasaki University Hospital"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroo","family":"Nakagawa","sequence":"additional","affiliation":[{"name":"Department of Hospital Pharmacy, Nagasaki University Hospital"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takahiro","family":"Muro","sequence":"additional","affiliation":[{"name":"Department of Hospital Pharmacy, Nagasaki University Hospital"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tadahiro","family":"Nakamura","sequence":"additional","affiliation":[{"name":"Department of Hospital Pharmacy, Nagasaki University Hospital"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Kitahara","sequence":"additional","affiliation":[{"name":"Department of Hospital Pharmacy, Nagasaki University Hospital"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hitoshi","family":"Sasaki","sequence":"additional","affiliation":[{"name":"Department of Hospital Pharmacy, Nagasaki University Hospital"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"163","reference":[{"key":"1","unstructured":"1) Edelstein ML, Abedi MR, Wixon J. Gene therapy clinical trials worldwide to 2007\u2014an update. <i>J. Gene Med.<\/i>, 9, 833\u2013842 (2007)."},{"key":"2","unstructured":"2) Bader AG, Brown D, Stoudemire J, Lammers P. Developing therapeutic microRNAs for cancer. <i>Gene Ther.<\/i>, 18, 1121\u20131126 (2011)."},{"key":"3","unstructured":"3) Ginn SL, Alexander IE, Edelstein ML, Abedi MR, Wixon J. Gene therapy clinical trials worldwide to 2012\u2014an update. <i>J. Gene Med.<\/i>, 15, 65\u201377 (2013)."},{"key":"4","unstructured":"4) Fiedler J, Thum T. MicroRNAs in myocardial infarction. <i>Arterioscler. Thromb. Vasc. Biol.<\/i>, 33, 201\u2013205 (2013)."},{"key":"5","unstructured":"5) Cho HJ, Chong S, Chung SJ, Shim CK, Kim DD. Poly-L-arginine and dextran sulfate-based nanocomplex for epidermal growth factor receptor (EGFR) siRNA delivery: its application for head and neck cancer treatment. <i>Pharm. Res.<\/i>, 29, 1007\u20131019 (2012)."},{"key":"6","doi-asserted-by":"crossref","unstructured":"6) Zhao ZX, Gao SY, Wang JC, Chen CJ, Zhao EY, Hou WJ, Feng Q, Gao LY, Liu XY, Zhang LR, Zhang Q. Self-assembly nanomicelles based on cationic mPEG-PLA-<i>b<\/i>-polyarginine (R15) triblock copolymer for siRNA delivery. <i>Biomaterials<\/i>, 33, 6793\u20136807 (2012).","DOI":"10.1016\/j.biomaterials.2012.05.067"},{"key":"7","unstructured":"7) Layek B, Singh J. Caproic acid grafted chitosan cationic nanocomplexes for enhanced gene delivery: effect of degree of substitution. <i>Int. J. Pharm.<\/i>, 447, 182\u2013191 (2013)."},{"key":"8","doi-asserted-by":"crossref","unstructured":"8) Li X, Chen Y, Wang M, Ma Y, Xia W, Gu H. A mesoporous silica nanoparticle\u2013PEI\u2013fusogenic peptide system for siRNA delivery in cancer therapy. <i>Biomaterials<\/i>, 34, 1391\u20131401 (2013).","DOI":"10.1016\/j.biomaterials.2012.10.072"},{"key":"9","unstructured":"9) Kitchens KM, El-Sayed ME, Ghandehari H. Transepithelial and endothelial transport of poly(amidoamine) dendrimers. <i>Adv. Drug Deliv. Rev.<\/i>, 57, 2163\u20132176 (2005)."},{"key":"10","unstructured":"10) Caminade AM, Laurent R, Majoral JP. Characterization of dendrimers. <i>Adv. Drug Deliv. Rev.<\/i>, 57, 2130\u20132146 (2005)."},{"key":"11","unstructured":"11) Kukowska-Latallo JF, Bielinska AU, Johnson J, Spindler R, Tomalia DA, Baker JR Jr. Efficient transfer of genetic material into mammalian cells using Starburst polyamidoamine dendrimers. <i>Proc. Natl. Acad. Sci. U.S.A.<\/i>, 93, 4897\u20134902 (1996)."},{"key":"12","unstructured":"12) Maruyama-Tabata H, Harada Y, Matsumura T, Satoh E, Cui F, Iwai M, Kita M, Hibi S, Imanishi J, Sawada T, Mazda O. Effective suicide gene therapy <i>in vivo<\/i> by EBV-based plasmid vector coupled with polyamidoamine dendrimer. <i>Gene Ther.<\/i>, 7, 53\u201360 (2000)."},{"key":"13","doi-asserted-by":"crossref","unstructured":"13) Navarro G, Tros de Ilarduya C. Activated and non-activated PAMAM dendrimers for gene delivery <i>in vitro<\/i> and <i>in vivo.<\/i> <i>Nanomedicine<\/i>, 5, 287\u2013297 (2009).","DOI":"10.1016\/j.nano.2008.12.007"},{"key":"14","doi-asserted-by":"crossref","unstructured":"14) Wang Y, Kong W, Song Y, Duan Y, Wang L, Steinhoff G, Kong D, Yu Y. Polyamidoamine dendrimers with a modified Pentaerythritol core having high efficiency and low cytotoxicity as gene carriers. <i>Biomacromolecules<\/i>, 10, 617\u2013622 (2009).","DOI":"10.1021\/bm801333s"},{"key":"15","unstructured":"15) Naha PC, Davoren M, Lyng FM, Byrne HJ. Reactive oxygen species (ROS) induced cytokine production and cytotoxicity of PAMAM dendrimers in J774A.1 cells. <i>Toxicol. Appl. Pharmacol.<\/i>, 246, 91\u201399 (2010)."},{"key":"16","unstructured":"16) Mukherjee SP, Lyng FM, Garcia A, Davoren M, Byrne HJ. Mechanistic studies of <i>in vitro<\/i> cytotoxicity of poly(amidoamine) dendrimers in mammalian cells. <i>Toxicol. Appl. Pharmacol.<\/i>, 248, 259\u2013268 (2010)."},{"key":"17","unstructured":"17) Trubetskoy VS, Wong SC, Subbotin V, Budker VG, Loomis A, Hagstrom JE, Wolff JA. Recharging cationic DNA complexes with highly charged polyanions for <i>in vitro<\/i> and <i>in vivo<\/i> gene delivery. <i>Gene Ther.<\/i>, 10, 261\u2013271 (2003)."},{"key":"18","doi-asserted-by":"crossref","unstructured":"18) Ito T, Iida-Tanaka N, Niidome T, Kawano T, Kubo K, Yoshikawa K, Sato T, Yang Z, Koyama Y. Hyaluronic acid and its derivative as a multi-functional gene expression enhancer: protection from non-specific interactions, adhesion to targeted cells, and transcriptional activation. <i>J. Control. Release<\/i>, 112, 382\u2013388 (2006).","DOI":"10.1016\/j.jconrel.2006.03.013"},{"key":"19","doi-asserted-by":"crossref","unstructured":"19) Kurosaki T, Kitahara T, Fumoto S, Nishida K, Nakamura J, Niidome T, Kodama Y, Nakagawa H, To H, Sasaki H. Ternary complexes of pDNA, polyethylenimine, and gamma-polyglutamic acid for gene delivery systems. <i>Biomaterials<\/i>, 30, 2846\u20132853 (2009).","DOI":"10.1016\/j.biomaterials.2009.01.055"},{"key":"20","doi-asserted-by":"crossref","unstructured":"20) Hagiwara K, Nakata M, Koyama Y, Sato T. The effects of coating pDNA\/chitosan complexes with chondroitin sulfate on physicochemical characteristics and cell transfection. <i>Biomaterials<\/i>, 33, 7251\u20137260 (2012).","DOI":"10.1016\/j.biomaterials.2012.06.040"},{"key":"21","unstructured":"21) Kamata K, Takahashi M, Terajima K, Nishijima M. Spectrophotometric determination of sodium chondroitin sulfate in eye drops after derivatization with 4-amino-3-hydrazino-5-mercapto-1,2,4-triazole. <i>Analyst<\/i> (Lond.), 120, 2755\u20132758 (1995)."},{"key":"22","unstructured":"22) Cohen M, Wolfe R, Mai T, Lewis D. A randomized, double blind, placebo controlled trial of a topical cream containing glucosamine sulfate, chondroitin sulfate, and camphor for osteoarthritis of the knee. <i>J. Rheumatol.<\/i>, 30, 523\u2013528 (2003)."},{"key":"23","unstructured":"23) Oshika T, Okamoto F, Kaji Y, Hiraoka T, Kiuchi T, Sato M, Kawana K. Retention and removal of a new viscous dispersive ophthalmic viscosurgical device during cataract surgery in animal eyes. <i>Br. J. Ophthalmol.<\/i>, 90, 485\u2013487 (2006)."},{"key":"24","unstructured":"24) Sakai S, Otake E, Toida T, Goda Y. Identification of the origin of chondroitin sulfate in \u201chealth foods.\u201d <i>Chem. Pharm. Bull.<\/i>, 55, 299\u2013303 (2007)."},{"key":"25","doi-asserted-by":"crossref","unstructured":"25) Zukor K, He Z. Regenerative medicine: drawing breath after spinal injury. <i>Nature<\/i>, 475, 177\u2013178 (2011).","DOI":"10.1038\/475178a"},{"key":"26","doi-asserted-by":"crossref","unstructured":"26) Iovu M, Dumais G, du Souich P. Anti-inflammatory activity of chondroitin sulfate. <i>Osteoarthritis Cartilage<\/i>, 16 (Suppl. 3), S14\u2013S18 (2008).","DOI":"10.1016\/j.joca.2008.06.008"},{"key":"27","unstructured":"27) Asimakopoulou AP, Theocharis AD, Tzanakakis GN, Karamanos NK. The biological role of chondroitin sulfate in cancer and chondroitin-based anticancer agents. <i>In Vivo<\/i>, 22, 385\u2013389 (2008)."},{"key":"28","unstructured":"28) Yang H, Liu S, Cai H, Wan L, Li S, Li Y, Cheng J, Lu X. Chondroitin sulfate as a molecular portal that preferentially mediates the apoptotic killing of tumor cells by penetratin-directed mitochondria-disrupting peptides. <i>J. Biol. Chem.<\/i>, 285, 25666\u201325676 (2010)."},{"key":"29","unstructured":"29) Tang MX, Szoka FC. The influence of polymer structure on the interactions of cationic polymers with DNA and morphology of the resulting complexes. <i>Gene Ther.<\/i>, 4, 823\u2013832 (1997)."},{"key":"30","unstructured":"30) Filion MC, Phillips NC. Major limitations in the use of cationic liposomes for DNA delivery. <i>Int. J. Pharm.<\/i>, 162, 159\u2013170 (1998)."},{"key":"31","unstructured":"31) Gao X, Kim KS, Liu D. Nonviral gene delivery: what we know and what is next. <i>AAPS J.<\/i>, 9, E92\u2013E104 (2007)."},{"key":"32","unstructured":"32) Shcharbin DG, Klajnert B, Bryszewska M. Dendrimers in gene transfection. <i>Biochemistry<\/i> (Mosc.), 74, 1070\u20131079 (2009)."},{"key":"33","unstructured":"33) Jain K, Kesharwani P, Gupta U, Jain NK. Dendrimer toxicity: Let\u2019s meet the challenge. <i>Int. J. Pharm.<\/i>, 394, 122\u2013142 (2010)."},{"key":"34","doi-asserted-by":"crossref","unstructured":"34) Tomalia DA. Dendrimer molecules. <i>Scientific American<\/i>, 272, 62\u201366 (1995).","DOI":"10.1038\/scientificamerican0595-62"},{"key":"35","unstructured":"35) Bielinska AU, Kukowska-Latallo JF, Baker JR Jr. The interaction of plasmid DNA with polyamidoamine dendrimers: mechanism of complex formation and analysis of alterations induced in nuclease sensitivity and transcriptional activity of the complexed DNA. <i>Biochim. Biophys. Acta<\/i>, 1353, 180\u2013190 (1997)."},{"key":"36","unstructured":"36) Zhou J, Wu J, Hafdi N, Behr JP, Erbacher P, Peng L. PAMAM dendrimers for efficient siRNA delivery and potent gene silencing. <i>Chem. Commun.<\/i> (Camb.), 14, 2362\u20132364 (2006)."},{"key":"37","unstructured":"37) Jevprasesphant R, Penny J, Jalal R, Attwood D, McKeown NB, D\u2019Emanuele A. The influence of surface modification on the cytotoxicity of PAMAM dendrimers. <i>Int. J. Pharm.<\/i>, 252, 263\u2013266 (2003)."},{"key":"38","doi-asserted-by":"crossref","unstructured":"38) Mukherjee SP, Davoren M, Byrne HJ. <i>In vitro<\/i> mammalian cytotoxicological study of PAMAM dendrimers\u2014towards quantitative structure activity relationships. <i>Toxicol. In Vitro<\/i>, 24, 169\u2013177 (2010).","DOI":"10.1016\/j.tiv.2009.09.014"},{"key":"39","unstructured":"39) Li S, Rizzo MA, Bhattacharya S, Huang L. Characterization of cationic lipid\u2013protamine\u2013DNA (LPD) complexes for intravenous gene delivery. <i>Gene Ther.<\/i>, 5, 930\u2013937 (1998)."},{"key":"40","unstructured":"40) Sakurai F, Nishioka T, Yamashita F, Takakura Y, Hashida M. Effects of erythrocytes and serum proteins on lung accumulation of lipoplexes containing cholesterol or DOPE as a helper lipid in the single-pass rat lung perfusion system. <i>Eur. J. Pharm. Biopharm.<\/i>, 52, 165\u2013172 (2001)."},{"key":"41","unstructured":"41) Lee JH, Cha KE, Kim MS, Hong HW, Chung DJ, Ryu G, Myung H. Nanosized polyamidoamine (PAMAM) dendrimer-induced apoptosis mediated by mitochondrial dysfunction. <i>Toxicol. Lett.<\/i>, 190, 202\u2013207 (2009)."},{"key":"42","doi-asserted-by":"crossref","unstructured":"42) Roden L. <i>The Biochemistry of Glycoproteins and Proteoglycans<\/i>, (Lennarz WJ. ed.). Plenum Publishing, New York, pp. 267\u2013371 (1980).","DOI":"10.1007\/978-1-4684-1006-8"},{"key":"43","unstructured":"43) Greling H, Stuhlsatz W, Tillmanns U. <i>Methods of Enzymatic Analysis<\/i>, 3rd ed., Vol. 6. Verlag Chemie, Weinheim, pp. 60\u201369 (1984)."},{"key":"44","unstructured":"44) Ca\u00f1as N, Valero T, Villarroya M, Montell E, Verg\u00e9s J, Garc\u00eda AG, L\u00f3pez MG. Chondroitin sulfate protects SH-SY5Y cells from oxidative stress by inducing heme oxygenase-1 <i>via<\/i> phosphatidylinositol 3-kinase\/Akt. <i>J. Pharmacol. Exp. Ther.<\/i>, 323, 946\u2013953 (2007)."},{"key":"45","unstructured":"45) Campo GM, Avenoso A, Campo S, D\u2019Ascola A, Traina P, Sam\u00e0 D, Calatroni A. NF-kB and caspases are involved in the hyaluronan and chondroitin-4-sulphate-exerted antioxidant effect in fibroblast cultures exposed to oxidative stress. <i>J. Appl. Toxicol.<\/i>, 28, 509\u2013517 (2008)."},{"key":"46","unstructured":"46) Rejman J, Bragonzi A, Conese M. Role of clathrin- and caveolae-mediated endocytosis in gene transfer mediated by lipo- and polyplexes. <i>Mol. Ther.<\/i>, 12, 468\u2013474 (2005)."},{"key":"47","unstructured":"47) Lo YL, Sung KH, Chiu CC, Wang LF. Chemically conjugating polyethylenimine with chondroitin sulfate to promote CD44-mediated endocytosis for gene delivery. <i>Mol. Pharm.<\/i>, 10, 664\u2013676 (2013)."},{"key":"48","unstructured":"48) Kurosaki T, Yamashita Y, Aki K, Harasawa H, Nakagawa H, Kodama Y, Higuchi N, Nakamura T, Kitahara T, Sasaki H. Secure and effective gene vector of polyamidoamine dendrimer pharmaceutically modified with anionic polymer. <i>J. Pharm. Sci.<\/i>, 100, 4855\u20134863 (2011)."},{"key":"49","unstructured":"49) Kaplan ME, Coons AH, Deane HW. Localization of antigen in tissue cells; cellular distribution of pneumococcal polysaccharides types II and III in the mouse. <i>J. Exp. Med.<\/i>, 91, 15\u201330 (1949)."}],"container-title":["Biological and Pharmaceutical Bulletin"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jstage.jst.go.jp\/article\/bpb\/37\/4\/37_b13-00768\/_pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,3,16]],"date-time":"2021-03-16T05:38:47Z","timestamp":1615873127000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jstage.jst.go.jp\/article\/bpb\/37\/4\/37_b13-00768\/_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014]]},"references-count":49,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2014]]}},"URL":"https:\/\/doi.org\/10.1248\/bpb.b13-00768","relation":{},"ISSN":["0918-6158","1347-5215"],"issn-type":[{"value":"0918-6158","type":"print"},{"value":"1347-5215","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014]]}}}