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The pathogenesis of clostridial myonecrosis. <i>Int. J. Med. Microbiol.<\/i>, <b>290<\/b>, 497\u2013502 (2000).","DOI":"10.1016\/S1438-4221(00)80074-0"},{"key":"3","doi-asserted-by":"crossref","unstructured":"3) Weinstein L, Barza MA. Gas gangrene. <i>N. Engl. J. Med.<\/i>, <b>289<\/b>, 1129\u20131131 (1973).","DOI":"10.1056\/NEJM197311222892107"},{"key":"4","doi-asserted-by":"crossref","unstructured":"4) Bryant AE. Biology and pathogenesis of thrombosis and procoagulant activity in invasive infections caused by group A streptococci and <i>Clostridium perfringens.<\/i> <i>Clin. Microbiol. Rev.<\/i>, <b>16<\/b>, 451\u2013462 (2003).","DOI":"10.1128\/CMR.16.3.451-462.2003"},{"key":"5","doi-asserted-by":"crossref","unstructured":"5) Titball RW. 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Use of genetically manipulated strains of <i>Clostridium perfringens<\/i> reveals that both alpha-toxin and theta-toxin are required for vascular leukostasis to occur in experimental gas gangrene. <i>Infect. Immun.<\/i>, <b>67<\/b>, 4902\u20134907 (1999).","DOI":"10.1128\/IAI.67.9.4902-4907.1999"},{"key":"9","doi-asserted-by":"crossref","unstructured":"9) Stevens DL, Troyer BE, Merrick DT, Mitten JE, Olson RD. Lethal effects and cardiovascular effects of purified alpha- and theta-toxins from <i>Clostridium perfringens.<\/i> <i>J. Infect. Dis.<\/i>, <b>157<\/b>, 272\u2013279 (1988).","DOI":"10.1093\/infdis\/157.2.272"},{"key":"10","doi-asserted-by":"crossref","unstructured":"10) Nagahama M, Okagawa Y, Nakayama T, Nishioka E, Sakurai J. Site-directed mutagenesis of histidine residues in <i>Clostridium perfringens<\/i> alpha-toxin. <i>J. Bacteriol.<\/i>, <b>177<\/b>, 1179\u20131185 (1995).","DOI":"10.1128\/jb.177.5.1179-1185.1995"},{"key":"11","doi-asserted-by":"crossref","unstructured":"11) Takehara M, Takagishi T, Seike S, Ohtani K, Kobayashi K, Miyamoto K, Shimizu T, Nagahama M. <i>Clostridium perfringens<\/i> alpha-toxin impairs innate immunity <i>via<\/i> inhibition of neutrophil differentiation. <i>Sci. Rep.<\/i>, <b>6<\/b>, 28192 (2016).","DOI":"10.1038\/srep28192"},{"key":"12","doi-asserted-by":"crossref","unstructured":"12) Diaz O, Mebarek-Azzam S, Benzaria A, Dubois M, Lagarde M, Nemoz G, Prigent AF. Disruption of lipid rafts stimulates phospholipase d activity in human lymphocytes: implication in the regulation of immune function. <i>J. Immunol.<\/i>, <b>175<\/b>, 8077\u20138086 (2005).","DOI":"10.4049\/jimmunol.175.12.8077"},{"key":"13","doi-asserted-by":"crossref","unstructured":"13) Ostrom RS, Insel PA. The evolving role of lipid rafts and caveolae in G protein-coupled receptor signaling: implications for molecular pharmacology. <i>Br. J. Pharmacol.<\/i>, <b>143<\/b>, 235\u2013245 (2004).","DOI":"10.1038\/sj.bjp.0705930"},{"key":"14","doi-asserted-by":"crossref","unstructured":"14) Simons K, Toomre D. Lipid rafts and signal transduction. <i>Nat. Rev. Mol. Cell Biol.<\/i>, <b>1<\/b>, 31\u201339 (2000).","DOI":"10.1038\/35036052"},{"key":"15","doi-asserted-by":"crossref","unstructured":"15) Tansey MG, Baloh RH, Milbrandt J, Johnson EM Jr. GFRalpha-mediated localization of RET to lipid rafts is required for effective downstream signaling, differentiation, and neuronal survival. <i>Neuron<\/i>, <b>25<\/b>, 611\u2013623 (2000).","DOI":"10.1016\/S0896-6273(00)81064-8"},{"key":"16","doi-asserted-by":"crossref","unstructured":"16) Konstantinidis DG, Pushkaran S, Johnson JF, Cancelas JA, Manganaris S, Harris CE, Williams DA, Zheng Y, Kalfa TA. 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Mutations in the neutral sphingomyelinase gene SMPD3 implicate the ceramide pathway in human leukemias. <i>Blood<\/i>, <b>111<\/b>, 4716\u20134722 (2008).","DOI":"10.1182\/blood-2007-10-113068"},{"key":"28","doi-asserted-by":"crossref","unstructured":"28) Milhas D, Clarke CJ, Hannun YA. Sphingomyelin metabolism at the plasma membrane: implications for bioactive sphingolipids. <i>FEBS Lett.<\/i>, <b>584<\/b>, 1887\u20131894 (2010).","DOI":"10.1016\/j.febslet.2009.10.058"},{"key":"29","doi-asserted-by":"crossref","unstructured":"29) Bollinger CR, Teichgraber V, Gulbins E. Ceramide-enriched membrane domains. <i>Biochim. Biophys. Acta<\/i>, <b>1746<\/b>, 284\u2013294 (2005).","DOI":"10.1016\/j.bbamcr.2005.09.001"},{"key":"30","doi-asserted-by":"crossref","unstructured":"30) Kolesnick RN, Kronke M. Regulation of ceramide production and apoptosis. <i>Annu. Rev. 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