{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T07:33:28Z","timestamp":1792654408768,"version":"4.5.0"},"reference-count":34,"publisher":"Pharmaceutical Society of Japan","issue":"8","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biological &amp; Pharmaceutical Bulletin","Biol. Pharm. Bull."],"published-print":{"date-parts":[[2017]]},"DOI":"10.1248\/bpb.b17-00244","type":"journal-article","created":{"date-parts":[[2017,7,31]],"date-time":"2017-07-31T22:14:31Z","timestamp":1501539271000},"page":"1299-1305","source":"Crossref","is-referenced-by-count":15,"title":["Chlorpheniramine Increases Paracellular Permeability to Marker Fluorescein Lucifer Yellow Mediated by Internalization of Occludin in Murine Colonic Epithelial Cells"],"prefix":"10.1248","volume":"40","author":[{"given":"Aya","family":"Manabe","sequence":"first","affiliation":[{"name":"From the Laboratory of Biochemistry, Department of Biopharmaceutical Sciences, Gifu Pharmaceutical University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chisa","family":"Furukawa","sequence":"additional","affiliation":[{"name":"From the Laboratory of Biochemistry, Department of Biopharmaceutical Sciences, Gifu Pharmaceutical University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoshi","family":"Endo","sequence":"additional","affiliation":[{"name":"From the Laboratory of Biochemistry, Department of Biopharmaceutical Sciences, Gifu Pharmaceutical University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kana","family":"Marunaka","sequence":"additional","affiliation":[{"name":"From the Laboratory of Biochemistry, Department of Biopharmaceutical Sciences, Gifu Pharmaceutical University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tsubasa","family":"Nishiyama","sequence":"additional","affiliation":[{"name":"From the Laboratory of Biochemistry, Department of Biopharmaceutical Sciences, Gifu Pharmaceutical University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoko","family":"Fujii","sequence":"additional","affiliation":[{"name":"From the Laboratory of Biochemistry, Department of Biopharmaceutical Sciences, Gifu Pharmaceutical University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshiaki","family":"Tabuchi","sequence":"additional","affiliation":[{"name":"Life Science Research Center, University of Toyama"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshiyuki","family":"Matsunaga","sequence":"additional","affiliation":[{"name":"From the Laboratory of Biochemistry, Department of Biopharmaceutical Sciences, Gifu Pharmaceutical University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akira","family":"Ikari","sequence":"additional","affiliation":[{"name":"From the Laboratory of Biochemistry, Department of Biopharmaceutical Sciences, Gifu Pharmaceutical University"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"163","reference":[{"key":"1","doi-asserted-by":"crossref","unstructured":"1) Proctor WR, Ming X, Bourdet D, Han TK, Everett RS, Thakker DR. Why does the intestine lack basolateral efflux transporters for cationic compounds? A provocative hypothesis. <i>J. Pharm. Sci.<\/i>, <b>105<\/b>, 484\u2013496 (2016).","DOI":"10.1016\/j.xphs.2015.11.040"},{"key":"2","unstructured":"2) Terada T, Hira D. Intestinal and hepatic drug transporters: pharmacokinetic, pathophysiological, and pharmacogenetic roles. <i>J. Gastroenterol.<\/i>, <b>50<\/b>, 508\u2013519 (2015)."},{"key":"3","doi-asserted-by":"crossref","unstructured":"3) Nakanishi T, Tamai I. Interaction of drug or food with drug transporters in intestine and liver. <i>Curr. Drug Metab.<\/i>, <b>16<\/b>, 753\u2013764 (2015).","DOI":"10.2174\/138920021609151201113537"},{"key":"4","doi-asserted-by":"crossref","unstructured":"4) Takenaka T, Harada N, Kuze J, Chiba M, Iwao T, Matsunaga T. Application of a human intestinal epithelial cell monolayer to the prediction of oral drug absorption in humans as a superior alternative to the Caco-2 cell monolayer. <i>J. Pharm. Sci.<\/i>, <b>105<\/b>, 915\u2013924 (2016).","DOI":"10.1016\/j.xphs.2015.11.035"},{"key":"5","doi-asserted-by":"crossref","unstructured":"5) Tsukita S, Furuse M, Itoh M. Multifunctional strands in tight junctions. <i>Nat. Rev. Mol. Cell Biol.<\/i>, <b>2<\/b>, 285\u2013293 (2001).","DOI":"10.1038\/35067088"},{"key":"6","doi-asserted-by":"crossref","unstructured":"6) Furuse M. Knockout animals and natural mutations as experimental and diagnostic tool for studying tight junction functions <i>in vivo<\/i>. <i>Biochim. Biophys. Acta<\/i>, <b>1788<\/b>, 813\u2013819 (2009).","DOI":"10.1016\/j.bbamem.2008.07.017"},{"key":"7","doi-asserted-by":"crossref","unstructured":"7) Hou J, Gomes AS, Paul DL, Goodenough DA. Study of claudin function by RNA interference. <i>J. Biol. Chem.<\/i>, <b>281<\/b>, 36117\u201336123 (2006).","DOI":"10.1074\/jbc.M608853200"},{"key":"8","doi-asserted-by":"crossref","unstructured":"8) Van Itallie CM, Anderson JM. Claudins and epithelial paracellular transport. <i>Annu. Rev. Physiol.<\/i>, <b>68<\/b>, 403\u2013429 (2006).","DOI":"10.1146\/annurev.physiol.68.040104.131404"},{"key":"9","doi-asserted-by":"crossref","unstructured":"9) Mineta K, Yamamoto Y, Yamazaki Y, Tanaka H, Tada Y, Saito K, Tamura A, Igarashi M, Endo T, Takeuchi K, Tsukita S. Predicted expansion of the claudin multigene family. <i>FEBS Lett.<\/i>, <b>585<\/b>, 606\u2013612 (2011).","DOI":"10.1016\/j.febslet.2011.01.028"},{"key":"10","doi-asserted-by":"crossref","unstructured":"10) Turksen K, Troy TC. Barriers built on claudins. <i>J. Cell Sci.<\/i>, <b>117<\/b>, 2435\u20132447 (2004).","DOI":"10.1242\/jcs.01235"},{"key":"11","doi-asserted-by":"crossref","unstructured":"11) Yu AS, McCarthy KM, Francis SA, McCormack JM, Lai J, Rogers RA, Lynch RD, Schneeberger EE. Knockdown of occludin expression leads to diverse phenotypic alterations in epithelial cells. <i>Am. J. Physiol. Cell Physiol.<\/i>, <b>288<\/b>, C1231\u2013C1241 (2005).","DOI":"10.1152\/ajpcell.00581.2004"},{"key":"12","doi-asserted-by":"crossref","unstructured":"12) Okubo K, Kurono Y, Fujieda S, Ogino S, Uchio E, Odajima H, Takenaka H, Japanese Society of Allergology. Japanese Guideline for Allergic Rhinitis 2014. <i>Allergol. Int.<\/i>, <b>63<\/b>, 357\u2013375 (2014).","DOI":"10.2332\/allergolint.14-RAI-0768"},{"key":"13","unstructured":"13) Mizuuchi H, Katsura T, Saito H, Hashimoto Y, Inui KI. Transport characteristics of diphenhydramine in human intestinal epithelial Caco-2 cells: contribution of pH-dependent transport system. <i>J. Pharmacol. Exp. Ther.<\/i>, <b>290<\/b>, 388\u2013392 (1999)."},{"key":"14","unstructured":"14) Fischer W, Neubert RH, Brandsch M. Transport of phenylethylamine at intestinal epithelial (Caco-2) cells: mechanism and substrate specificity. <i>Eur. J. Pharm. Biopharm.<\/i>, <b>74<\/b>, 281\u2013289 (2010)."},{"key":"15","doi-asserted-by":"crossref","unstructured":"15) Tabuchi Y, Ohta S, Arai Y, Kawahara M, Ishibashi K, Sugiyama N, Horiuchi T, Furusawa M, Obinata M, Fuse H, Takeguchi N, Asano S. Establishment and characterization of a colonic epithelial cell line MCE301 from transgenic mice harboring temperature-sensitive simian virus 40 large T-antigen gene. <i>Cell Struct. Funct.<\/i>, <b>25<\/b>, 297\u2013307 (2000).","DOI":"10.1247\/csf.25.297"},{"key":"16","doi-asserted-by":"crossref","unstructured":"16) Takai A, Ohno Y, Yasumoto T, Mieskes G. Estimation of the rate constants associated with the inhibitory effect of okadaic acid on type 2A protein phosphatase by time\u2013course analysis. <i>Biochem. J.<\/i>, <b>287<\/b>, 101\u2013106 (1992).","DOI":"10.1042\/bj2870101"},{"key":"17","doi-asserted-by":"crossref","unstructured":"17) Holmes JL, Van Itallie CM, Rasmussen JE, Anderson JM. Claudin profiling in the mouse during postnatal intestinal development and along the gastrointestinal tract reveals complex expression patterns. <i>Gene Expr. Patterns<\/i>, <b>6<\/b>, 581\u2013588 (2006).","DOI":"10.1016\/j.modgep.2005.12.001"},{"key":"18","doi-asserted-by":"crossref","unstructured":"18) Nusrat A, Parkos CA, Verkade P, Foley CS, Liang TW, Innis-Whitehouse W, Eastburn KK, Madara JL. Tight junctions are membrane microdomains. <i>J. Cell Sci.<\/i>, <b>113<\/b>, 1771\u20131781 (2000).","DOI":"10.1242\/jcs.113.10.1771"},{"key":"19","doi-asserted-by":"crossref","unstructured":"19) Clarke H, Soler AP, Mullin JM. Protein kinase C activation leads to dephosphorylation of occludin and tight junction permeability increase in LLC-PK1 epithelial cell sheets. <i>J. Cell Sci.<\/i>, <b>113<\/b>, 3187\u20133196 (2000).","DOI":"10.1242\/jcs.113.18.3187"},{"key":"20","doi-asserted-by":"crossref","unstructured":"20) Takenaka T, Harada N, Kuze J, Chiba M, Iwao T, Matsunaga T. Human small intestinal epithelial cells differentiated from adult intestinal stem cells as a novel system for predicting oral drug absorption in humans. <i>Drug Metab. Dispos.<\/i>, <b>42<\/b>, 1947\u20131954 (2014).","DOI":"10.1124\/dmd.114.059493"},{"key":"21","doi-asserted-by":"crossref","unstructured":"21) Sj\u00f6berg A, Lutz M, Tannergren C, Wingolf C, Borde A, Ungell AL. Comprehensive study on regional human intestinal permeability and prediction of fraction absorbed of drugs using the Ussing chamber technique. <i>Eur. J. Pharm. Sci.<\/i>, <b>48<\/b>, 166\u2013180 (2013).","DOI":"10.1016\/j.ejps.2012.10.007"},{"key":"22","doi-asserted-by":"crossref","unstructured":"22) Hou J, Renigunta A, Yang J, Waldegger S. Claudin-4 forms paracellular chloride channel in the kidney and requires claudin-8 for tight junction localization. <i>Proc. Natl. Acad. Sci. U.S.A.<\/i>, <b>107<\/b>, 18010\u201318015 (2010).","DOI":"10.1073\/pnas.1009399107"},{"key":"23","doi-asserted-by":"crossref","unstructured":"23) Piehl C, Piontek J, Cording J, Wolburg H, Blasig IE. Participation of the second extracellular loop of claudin-5 in paracellular tightening against ions, small and large molecules. <i>Cell. Mol. Life Sci.<\/i>, <b>67<\/b>, 2131\u20132140 (2010).","DOI":"10.1007\/s00018-010-0332-8"},{"key":"24","doi-asserted-by":"crossref","unstructured":"24) Markov AG, Veshnyakova A, Fromm M, Amasheh M, Amasheh S. Segmental expression of claudin proteins correlates with tight junction barrier properties in rat intestine. <i>J. Comp. Physiol. B<\/i>, <b>180<\/b>, 591\u2013598 (2010).","DOI":"10.1007\/s00360-009-0440-7"},{"key":"25","doi-asserted-by":"crossref","unstructured":"25) Saitou M, Furuse M, Sasaki H, Schulzke JD, Fromm M, Takano H, Noda T, Tsukita S. Complex phenotype of mice lacking occludin, a component of tight junction strands. <i>Mol. Biol. Cell<\/i>, <b>11<\/b>, 4131\u20134142 (2000).","DOI":"10.1091\/mbc.11.12.4131"},{"key":"26","doi-asserted-by":"crossref","unstructured":"26) Schulzke JD, Gitter AH, Mankertz J, Spiegel S, Seidler U, Amasheh S, Saitou M, Tsukita S, Fromm M. Epithelial transport and barrier function in occludin-deficient mice. <i>Biochim. Biophys. Acta<\/i>, <b>1669<\/b>, 34\u201342 (2005).","DOI":"10.1016\/j.bbamem.2005.01.008"},{"key":"27","doi-asserted-by":"crossref","unstructured":"27) Watari A, Hasegawa M, Yagi K, Kondoh M. Checkpoint kinase 1 activation enhances intestinal epithelial barrier function <i>via<\/i> regulation of claudin-5 expression. <i>PLOS ONE<\/i>, <b>11<\/b>, e0145631 (2016).","DOI":"10.1371\/journal.pone.0145631"},{"key":"28","doi-asserted-by":"crossref","unstructured":"28) Hering NA, Andres S, Fromm A, van Tol EA, Amasheh M, Mankertz J, Fromm M, Schulzke JD. Transforming growth factor-beta, a whey protein component, strengthens the intestinal barrier by upregulating claudin-4 in HT-29\/B6 cells. <i>J. Nutr.<\/i>, <b>141<\/b>, 783\u2013789 (2011).","DOI":"10.3945\/jn.110.137588"},{"key":"29","doi-asserted-by":"crossref","unstructured":"29) Ohta H, Yamaguchi T, Rajapakshage BK, Murakami M, Sasaki N, Nakamura K, Hwang SJ, Yamasaki M, Takiguchi M. Expression and subcellular localization of apical junction proteins in canine duodenal and colonic mucosa. <i>Am. J. Vet. Res.<\/i>, <b>72<\/b>, 1046\u20131051 (2011).","DOI":"10.2460\/ajvr.72.8.1046"},{"key":"30","doi-asserted-by":"crossref","unstructured":"30) Carrasco-Pozo C, Morales P, Gotteland M. Polyphenols protect the epithelial barrier function of Caco-2 cells exposed to indomethacin through the modulation of occludin and zonula occludens-1 expression. <i>J. Agric. Food Chem.<\/i>, <b>61<\/b>, 5291\u20135297 (2013).","DOI":"10.1021\/jf400150p"},{"key":"31","doi-asserted-by":"crossref","unstructured":"31) Park HY, Kunitake Y, Hirasaki N, Tanaka M, Matsui T. Theaflavins enhance intestinal barrier of Caco-2 cell monolayers through the expression of AMP-activated protein kinase-mediated occludin, claudin-1, and ZO-1. <i>Biosci. Biotechnol. Biochem.<\/i>, <b>79<\/b>, 130\u2013137 (2015).","DOI":"10.1080\/09168451.2014.951027"},{"key":"32","doi-asserted-by":"crossref","unstructured":"32) Nunbhakdi-Craig V, Machleidt T, Ogris E, Bellotto D, White CL 3rd, Sontag E. Protein phosphatase 2A associates with and regulates atypical PKC and the epithelial tight junction complex. <i>J. Cell Biol.<\/i>, <b>158<\/b>, 967\u2013978 (2002).","DOI":"10.1083\/jcb.200206114"},{"key":"33","doi-asserted-by":"crossref","unstructured":"33) Rao R. Occludin phosphorylation in regulation of epithelial tight junctions. <i>Ann. N. Y. Acad. Sci.<\/i>, <b>1165<\/b>, 62\u201368 (2009).","DOI":"10.1111\/j.1749-6632.2009.04054.x"},{"key":"34","doi-asserted-by":"crossref","unstructured":"34) Sander LE, Lorentz A, Sellge G, Coeffier M, Neipp M, Veres T, Frieling T, Meier PN, Manns MP, Bischoff SC. Selective expression of histamine receptors H1R, H2R, and H4R, but not H3R, in the human intestinal tract. <i>Gut<\/i>, <b>55<\/b>, 498\u2013504 (2006).","DOI":"10.1136\/gut.2004.061762"}],"container-title":["Biological &amp; Pharmaceutical Bulletin","Biological and Pharmaceutical Bulletin"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jstage.jst.go.jp\/article\/bpb\/40\/8\/40_b17-00244\/_pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,7,31]],"date-time":"2022-07-31T17:18:53Z","timestamp":1659287933000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jstage.jst.go.jp\/article\/bpb\/40\/8\/40_b17-00244\/_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017]]},"references-count":34,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2017]]}},"URL":"https:\/\/doi.org\/10.1248\/bpb.b17-00244","relation":{},"ISSN":["0918-6158","1347-5215"],"issn-type":[{"value":"0918-6158","type":"print"},{"value":"1347-5215","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017]]}}}