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Pueraria tuberosa<\/i>: a review on its phytochemical and therapeutic potential. Nat. Prod. Res.<\/i>, 28<\/b>, 2111\u20132127 (2014).","DOI":"10.1080\/14786419.2014.928291"},{"key":"2","doi-asserted-by":"crossref","unstructured":"2) H\u00e4m\u00e4l\u00e4inen M, Nieminen R, Vuorela P, Heinonen M, Moilanen E. Anti-inflammatory effects of flavonoids: genistein, kaempferol, quercetin, and daidzein inhibit STAT-1 and NF-\u03baB activations, whereas flavone, isorhamnetin, naringenin, and pelargonidin inhibit only NF-\u03baB activation along with their inhibitory effect on iNOS expression and NO production in activated macrophages. Mediators Inflamm.<\/i>, 2007<\/b>, 45673 (2007).","DOI":"10.1155\/2007\/45673"},{"key":"3","doi-asserted-by":"crossref","unstructured":"3) Wang D, Bu Z, Li Y, He Y, Yang F, Zou L. Zou. Pharmacological activity, pharmacokinetics, and clinical research progress of puerarin. Antioxidants <\/i>(Basel), 11<\/b>, 2121 (2022).","DOI":"10.3390\/antiox11112121"},{"key":"4","doi-asserted-by":"crossref","unstructured":"4) Xie J, Yang F, Shi X, Zhu X, Su W, Wang P. Improvement in solubility and bioavailability of puerarin by mechanochemical preparation. Drug Dev. Ind. Pharm.<\/i>, 39<\/b>, 826\u2013835 (2013).","DOI":"10.3109\/03639045.2012.664147"},{"key":"5","doi-asserted-by":"crossref","unstructured":"5) Li H, Dong L, Liu Y, Wang G, Wang G, Qiao Y. Biopharmaceutics classification of puerarin and comparison of perfusion approaches in rats. Int. J. Pharm.<\/i>, 466<\/b>, 133\u2013138 (2014).","DOI":"10.1016\/j.ijpharm.2014.03.014"},{"key":"6","doi-asserted-by":"crossref","unstructured":"6) Meng F, Guo B, Ma YQ, Li KW, Niu FJ. Puerarin: a review of its mechanisms of action and clinical studies in ophthalmology. Phytomedicine<\/i>, 107<\/b>, 154465 (2022).","DOI":"10.1016\/j.phymed.2022.154465"},{"key":"7","doi-asserted-by":"crossref","unstructured":"7) Luo CF, Yuan M, Chen MS, Liu SM, Zhu L, Huang BY, Liu XW, Xiong W. Pharmacokinetics, tissue distribution and relative bioavailability of puerarin solid lipid nanoparticles following oral administration. Int. J. Pharm.<\/i>, 410<\/b>, 138\u2013144 (2011).","DOI":"10.1016\/j.ijpharm.2011.02.064"},{"key":"8","doi-asserted-by":"crossref","unstructured":"8) Tang TT, Hu XB, Liao DH, Liu XY, Xiang DX. Mechanisms of microemulsion enhancing the oral bioavailability of puerarin: comparison between oil-in-water and water-in-oil microemulsions using the single-pass intestinal perfusion method and a chylomicron flow blocking approach. Int. J. Nanomedicine<\/i>, 8<\/b>, 4415\u20134426 (2013).","DOI":"10.2147\/IJN.S51469"},{"key":"9","doi-asserted-by":"crossref","unstructured":"9) Tao HQ, Meng Q, Li MH, Yu H, Liu MF, Du D, Sun S, Yang H, Wang Y, Ye W, Yang L, Zhu D, Jiang C, Peng HS. HP-\u03b2-CD-PLGA nanoparticles improve the penetration and bioavailability of puerarin and enhance the therapeutic effects on brain ischemia\u2013reperfusion injury in rats. Naunyn Schmiedebergs Arch. Pharmacol.<\/i>, 386<\/b>, 61\u201370 (2013).","DOI":"10.1007\/s00210-012-0804-5"},{"key":"10","doi-asserted-by":"crossref","unstructured":"10) Zhang Y, Li Y, Zhao X, Zu Y, Wang W, Wu W, Li Z. Preparation, characterization and bioavailability of oral puerarin nanoparticles by emulsion solvent evaporation method. RSC Adv.<\/i>, 74<\/b>, 69889\u201369901 (2016).","DOI":"10.1039\/C6RA08413C"},{"key":"11","doi-asserted-by":"crossref","unstructured":"11) Zhao L, Liu A, Sun M, Gu J, Wang H, Wang S, Zhai G. Enhancement of oral bioavailability of puerarin by poly-butyl-cyanoacrylate nanoparticles. J. Nanomater.<\/i>, 2011<\/b>, 1\u20138 (2011).","DOI":"10.1155\/2011\/126562"},{"key":"12","doi-asserted-by":"crossref","unstructured":"12) Chen AZ, Li Y, Chau FT, Lau TY, Hu JY, Zhao Z, Mok DK. Microencapsulation of puerarin nanoparticles by poly (L-lactide) in a supercritical CO2 process. Acta Biomater.<\/i>, 5<\/b>, 2913\u20132919 (2009).","DOI":"10.1016\/j.actbio.2009.04.032"},{"key":"13","doi-asserted-by":"crossref","unstructured":"13) Bharti R, Chopra BS, Raut S, Khatri N. Pueraria tuberosa: A review on traditional uses, pharmacology, and phytochemistry. Front. Pharmacol.<\/i>, 11<\/b>, 582506 (2021).","DOI":"10.3389\/fphar.2020.582506"},{"key":"14","doi-asserted-by":"crossref","unstructured":"14) Lin YY, Lin SC, Feng CW, Chen PC, Su YD, Li CM, Yang SN, Jean YH, Sung PJ, Duh CY, Wen ZH. Anti-inflammatory and analgesic effects of the marine-derived compound excavatolide B isolated from the culture-type Formosan gorgonian Briareum excavatum. Mar. Drugs<\/i>, 13<\/b>, 2559\u20132579 (2015).","DOI":"10.3390\/md13052559"},{"key":"15","doi-asserted-by":"crossref","unstructured":"15) Garcia Leme J, Hamamura L, Leite MP, Rocha e Silva M. Pharmacological analysis of the acute inflammatory process induced in the rat\u2019s paw by local injection of carrageenin and by heating. Br. J. Pharmacol.<\/i>, 48<\/b>, 88\u201396 (1973).","DOI":"10.1111\/j.1476-5381.1973.tb08225.x"},{"key":"16","doi-asserted-by":"crossref","unstructured":"16) Ullah MZ, Khan AU, Afridi R, Rasheed H, Khalid S, Naveed M, Ali H, Kim YS, Khan S. Attenuation of inflammatory pain by puerarin in animal model of inflammation through inhibition of pro-inflammatory mediators. Int. Immunopharmacol.<\/i>, 61<\/b>, 306\u2013316 (2018).","DOI":"10.1016\/j.intimp.2018.05.034"},{"key":"17","unstructured":"17) Ahirrao P, Deshmukh KN, Gupta A, Jachak SM. Formulation and characterization of puerarin loaded nanoparticles encapsulated in PDLG employing solvent evaporation method. J. Pharm. Res.<\/i>, in press."},{"key":"18","doi-asserted-by":"crossref","unstructured":"18) Desgouilles S, Vauthier C, Bazile D, Vacus J, Grossiord JL, Veillard M, Couvreur P. The design of nanoparticles obtained by solvent evaporation: a comprehensive study. Langmuir<\/i>, 19<\/b>, 9504\u20139510 (2003).","DOI":"10.1021\/la034999q"},{"key":"19","doi-asserted-by":"crossref","unstructured":"19) Bairwa K, Jachak SM. Anti-inflammatory potential of a lipid-based formulation of a rotenoid-rich fraction prepared from Boerhavia diffusa<\/i>. Pharm. Biol.<\/i>, 53<\/b>, 1231\u20131238 (2015).","DOI":"10.3109\/13880209.2014.971382"},{"key":"20","doi-asserted-by":"crossref","unstructured":"20) Tu L, Yi Y, Wu W, Hu F, Hu K, Feng J. Effects of particle size on the pharmacokinetics of puerarin nanocrystals and microcrystals after oral administration to rat. Int. J. Pharm.<\/i>, 458<\/b>, 135\u2013140 (2013).","DOI":"10.1016\/j.ijpharm.2013.10.001"},{"key":"21","doi-asserted-by":"crossref","unstructured":"21) Facchin BM, Dos Reis GO, Vieira GN, Mohr ETB, da Rosa JS, Kretzer IF, Demarchi IG, Dalmarco EM. Inflammatory biomarkers on an LPS-induced RAW 264.7 cell model: a systematic review and meta-analysis. Inflamm. Res.<\/i>, 71<\/b>, 741\u2013758 (2022).","DOI":"10.1007\/s00011-022-01584-0"},{"key":"22","doi-asserted-by":"crossref","unstructured":"22) Vijayalakshmi D, Dhandapani R, Jayaveni S, Jithendra PS, Rose C, Mandal AB. In vitro<\/i> anti-inflammatory activity of Aloe vera<\/i> by down regulation of MMP-9 in peripheral blood mononuclear cells. J. Ethnopharmacol.<\/i>, 141<\/b>, 542\u2013546 (2012).","DOI":"10.1016\/j.jep.2012.02.040"},{"key":"23","doi-asserted-by":"crossref","unstructured":"23) Kumar P, Nagarajan A, Uchil PD. Analysis of cell viability by the MTT assay. Cold Spring Harb. Protoc.<\/i>, 2018<\/b>, pdb.prot095505 (2018).","DOI":"10.1101\/pdb.prot095505"},{"key":"24","doi-asserted-by":"crossref","unstructured":"24) Park YM, Won JH, Yun KJ, Ryu JH, Han YN, Choi SK, Lee KT. Preventive effect of Ginkgo biloba<\/i> extract (GBB) on the lipopolysaccharide-induced expressions of inducible nitric oxide synthase and cyclooxygenase-2 via<\/i> suppression of nuclear factor-\u03baB in RAW 264.7 cells. Biol. Pharm. Bull.<\/i>, 29<\/b>, 985\u2013990 (2006).","DOI":"10.1248\/bpb.29.985"},{"key":"25","doi-asserted-by":"crossref","unstructured":"25) Nemudzivhadi V, Masoko P. In vitro<\/i> assessment of cytotoxicity, antioxidant, and anti-inflammatory activities of Ricinus communis<\/i> (Euphorbiaceae) leaf extracts. Evid. Based Complement. Alternat. Med.<\/i>, 2014<\/b>, 625961 (2014).","DOI":"10.1155\/2014\/625961"},{"key":"26","doi-asserted-by":"crossref","unstructured":"26) Yang EJ, Yim EY, Song GK, Kim GO, Hyun CG. Inhibition of nitric oxide production in lipopolysaccharide-activated RAW 264.7 macrophages by Jeju plant extracts. Interdiscip. Toxicol.<\/i>, 2<\/b>, 245\u2013249 (2009).","DOI":"10.2478\/v10102-009-0022-2"},{"key":"27","doi-asserted-by":"crossref","unstructured":"27) Garc\u00eda-Lafuente A, Moro C, Manch\u00f3n N, Gonzalo-Ruiz A, Villares A, Guillam\u00f3n E, Rostagno M, Mateo-Vivaracho L. In vitro<\/i> anti-inflammatory activity of phenolic rich extracts from white and red common beans. Food Chem.<\/i>, 161<\/b>, 216\u2013223 (2014).","DOI":"10.1016\/j.foodchem.2014.04.004"},{"key":"28","doi-asserted-by":"crossref","unstructured":"28) Vogl S, Picker P, Mihaly-Bison J, Fakhrudin N, Atanasov AG, Heiss EH, Wawrosch C, Reznicek G, Dirsch VM, Saukel J, Kopp B. Ethnopharmacological in vitro<\/i> studies on Austria\u2019s folk medicine\u2014an unexplored lore in vitro<\/i> anti-inflammatory activities of 71 Austrian traditional herbal drugs. J. Ethnopharmacol.<\/i>, 149<\/b>, 750\u2013771 (2013).","DOI":"10.1016\/j.jep.2013.06.007"},{"key":"29","doi-asserted-by":"crossref","unstructured":"29) Liu AC, Zhao LX, Xing J, Gao J, Lou HX. LC-MS\/MS method for the determination of a new puerarin derivative and its application in pharmacokinetic studies in rats. Chin. J. Nat. Med.<\/i>, 5<\/b>, 566\u2013571 (2013).","DOI":"10.1016\/S1875-5364(13)60102-8"},{"key":"30","doi-asserted-by":"crossref","unstructured":"30) Bairwa K, Singh IN, Roy SK, Grover J, Srivastava A, Jachak SM. Rotenoids from Boerhaavia diffusa<\/i> as potential anti-inflammatory agents. J. Nat. Prod.<\/i>, 76<\/b>, 1393\u20131398 (2013).","DOI":"10.1021\/np300899w"},{"key":"31","doi-asserted-by":"crossref","unstructured":"31) Krishnamoorthy B, Habibur Rahman SM, Tamil Selvan N, Hari Prasad R, Rajkumar M, Siva Selvakumar M, Vamshikrishna K, Gregory M, Vijayaraghavan C. Design, formulation, in-vitro, in-vivo<\/i>, and pharmacokinetic evaluation of nisoldipine-loaded self-nanoemulsifying drug delivery system. J. Nanopart. Res.<\/i>, 17<\/b>, 34(2015).","DOI":"10.1007\/s11051-014-2818-z"},{"key":"32","doi-asserted-by":"crossref","unstructured":"32) Machida R, Tokumura T, Tsuchiya Y, Sasaki A, Abe K. Pharmacokinetics of novel hexapeptides with neurotensin activity in rats. Biol. Pharm. Bull.<\/i>, 16<\/b>, 43\u201347 (1993).","DOI":"10.1248\/bpb.16.43"},{"key":"33","doi-asserted-by":"crossref","unstructured":"33) Ishii K, Katayama Y, Itai S, Ito Y, Hayashi H. A new pharmacokinetic model including in vivo<\/i> dissolution and gastrointestinal transit parameters. Biol. Pharm. Bull.<\/i>, 18<\/b>, 882\u2013886 (1995).","DOI":"10.1248\/bpb.18.882"},{"key":"34","doi-asserted-by":"crossref","unstructured":"34) Song X, Bai X, Liu S, Dong L, Deng H, Wang C. A novel microspheres formulation of puerarin: pharmacokinetics study and in vivo<\/i> pharmacodynamics evaluations. Evid. Based Complement. Alternat. Med.<\/i>, 1<\/b>, 4016963 (2016).","DOI":"10.1155\/2016\/4016963"},{"key":"35","doi-asserted-by":"crossref","unstructured":"35) Zhang G, Ji J, Sun M, Ji Y, Ji H. Comparative pharmacokinetic profiles of Puerarin in rat plasma by UHPLC-MS\/MS after oral administration of Pueraria lobata extract and pure Puerarin. J. Anal. Methods Chem.<\/i>, 1<\/b>, 4258156 (2020).","DOI":"10.1155\/2020\/4258156"},{"key":"36","doi-asserted-by":"crossref","unstructured":"36) Li Y, Pan WS, Chen SL, Xu HX, Yang DJ, Chan AC. Pharmacokinetic, tissue distribution, and excretion of puerarin and puerarin-phospholipid complex in rats. Drug Dev. Ind. Pharm.<\/i>, 4<\/b>, 413\u2013422 (2006).","DOI":"10.1080\/03639040600559123"},{"key":"37","doi-asserted-by":"crossref","unstructured":"37) Quan D, Xu X, Wu G. Studies on preparation and absolute bioavailability of a self-emulsifying system containing puerarin. Chem. Pharm. Bull.<\/i>, 55<\/b>, 800\u2013803 (2007).","DOI":"10.1248\/cpb.55.800"},{"key":"38","doi-asserted-by":"crossref","unstructured":"38) Jung HR, Kim J, Ham H, Cho H, Lee B, Cho Y. Simultaneous determination of puerarin and its active metabolite in human plasma by UPLC-MS\/MS: Application to a pharmacokinetic study. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci.<\/i>, 971<\/b>, 64\u201371 (2014).","DOI":"10.1016\/j.jchromb.2014.09.015"},{"key":"39","doi-asserted-by":"crossref","unstructured":"39) Saklani R, Tiwari K, Yadav K, Yadav P, Chourasia K. Validated HPLC-UV method for simultaneous estimation of paclitaxel and doxorubicin employing ion pair chromatography: application in formulation development and pharmacokinetic studies. BioMed Res. Int.<\/i>, 1<\/b>, 7708235 (2022).","DOI":"10.1155\/2022\/7708235"},{"key":"40","doi-asserted-by":"crossref","unstructured":"40) de Jes\u00fas Valle MJ, L\u00f3pez FG, Navarro AS. Development and validation of an HPLC method for vancomycin and its application to a pharmacokinetic study. J. Pharm. Biomed. Anal.<\/i>, 48<\/b>, 835\u2013839 (2008).","DOI":"10.1016\/j.jpba.2008.05.040"},{"key":"41","doi-asserted-by":"crossref","unstructured":"41) Bae JW, Kim MJ, Jang CG, Lee SY. Determination of meloxicam in human plasma using a HPLC method with UV detection and its application to a pharmacokinetic study. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci.<\/i>, 859<\/b>, 69\u201373 (2007).","DOI":"10.1016\/j.jchromb.2007.09.004"},{"key":"42","doi-asserted-by":"crossref","unstructured":"42) Matar KM, Nazi EM, El-Sayed YM, Al-Yamani MJ, Al-Suwayeh SA, Al-Khamis KI. Quantitative determination of clavulanic acid in plasma by HPLC: application to a pharmacokinetic study. J. Liq. Chromatogr. Relat. Technol.<\/i>, 28<\/b>, 97\u2013107 (2005).","DOI":"10.1081\/JLC-200038602"},{"key":"43","doi-asserted-by":"crossref","unstructured":"43) Garg Y, Kapoor DN, Sharma AK, Bhatia A. Drug delivery systems and strategies to overcome the barriers of brain. Curr. Pharm. Des.<\/i>, 28<\/b>, 619\u2013641 (2022).","DOI":"10.2174\/1381612828666211222163025"},{"key":"44","doi-asserted-by":"crossref","unstructured":"44) Mansouri MT, Hemmati AA, Naghizadeh B, Mard SA, Rezaie A, Ghorbanzadeh B. A study of the mechanisms underlying the anti-inflammatory effect of ellagic acid in carrageenan-induced paw edema in rats. Indian J. Pharmacol.<\/i>, 47<\/b>, 292\u2013298 (2015).","DOI":"10.4103\/0253-7613.157127"}],"container-title":["Biological and Pharmaceutical Bulletin"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jstage.jst.go.jp\/article\/bpb\/47\/12\/47_b24-00397\/_pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,28]],"date-time":"2024-12-28T03:29:34Z","timestamp":1735356574000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jstage.jst.go.jp\/article\/bpb\/47\/12\/47_b24-00397\/_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12,28]]},"references-count":44,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2024]]}},"URL":"https:\/\/doi.org\/10.1248\/bpb.b24-00397","relation":{},"ISSN":["0918-6158","1347-5215"],"issn-type":[{"value":"0918-6158","type":"print"},{"value":"1347-5215","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,12,28]]},"article-number":"b24-00397"}}