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Tissue-specific mutation accumulation in human adult stem cells during life. <i>Nature<\/i>, <b>538<\/b>, 260\u2013264 (2016).","DOI":"10.1038\/nature19768"},{"key":"2","doi-asserted-by":"crossref","unstructured":"2) Seithel A, Karlsson J, Hilgendorf C, Bj\u00f6rquist A, Ungell AL. Variability in mRNA expression of ABC- and SLC-transporters in human intestinal cells: comparison between human segments and Caco-2 cells. <i>Eur. J. Pharm. Sci.<\/i>, <b>28<\/b>, 291\u2013299 (2006).","DOI":"10.1016\/j.ejps.2006.03.003"},{"key":"3","doi-asserted-by":"crossref","unstructured":"3) Nakamura T, Sakaeda T, Ohmoto N, Tamura T, Aoyama N, Shirakawa T, Kamigaki T, Nakamura T, Kim KI, Kim SR, Kuroda Y, Matsuo M, Kasuga M, Okumura K. Real-time quantitative polymerase chain reaction for MDR1, MRP1, MRP2, and CYP3A-mRNA levels in Caco-2 cell lines, human duodenal enterocytes, normal colorectal tissues, and colorectal adenocarcinomas. <i>Drug Metab. Dispos.<\/i>, <b>30<\/b>, 4\u20136 (2002).","DOI":"10.1124\/dmd.30.1.4"},{"key":"4","doi-asserted-by":"crossref","unstructured":"4) Zhang Y, Huang S, Zhong W, Chen W, Yao B, Wang X. 3D organoids derived from the small intestine: an emerging tool for drug transport research. <i>Acta Pharm. Sin. B<\/i>, <b>11<\/b>, 1697\u20131707 (2021).","DOI":"10.1016\/j.apsb.2020.12.002"},{"key":"5","doi-asserted-by":"crossref","unstructured":"5) Xu H, Lyu X, Yi M, Zhao W, Song Y, Wu K. Organoid technology and applications in cancer research. <i>J. Hematol. Oncol.<\/i>, <b>11<\/b>, 116 (2018).","DOI":"10.1186\/s13045-018-0662-9"},{"key":"6","doi-asserted-by":"crossref","unstructured":"6) Kandilogiannakis L, Filidou E, Drygiannakis I, Tarapatzi G, Didaskalou S, Koffa M, Arvanitidis K, Bamias G, Valatas V, Paspaliaris V, Kolios G. Development of a human intestinal organoid model for <i>in vitro<\/i> studies on gut inflammation and fibrosis. <i>Stem Cells Int.<\/i>, <b>2021<\/b>, 9929461 (2021).","DOI":"10.1155\/2021\/9929461"},{"key":"7","doi-asserted-by":"crossref","unstructured":"7) Chen Y, Zhou W, Roh T, Estes MK, Kaplan DL. <i>In vitro<\/i> enteroid-derived three-dimensional tissue model of human small intestinal epithelium with innate immune responses. <i>PLOS ONE<\/i>, <b>12<\/b>, e0187880 (2017).","DOI":"10.1371\/journal.pone.0187880"},{"key":"8","doi-asserted-by":"crossref","unstructured":"8) Takahashi Y, Noguchi M, Inoue Y, Sato S, Shimizu M, Kojima H, Okabe T, Kiyono H, Yamauchi Y, Sato R. Organoid-derived intestinal epithelial cells are a suitable model for preclinical toxicology and pharmacokinetic studies. <i>iScience<\/i>, <b>25<\/b>, 104542 (2022).","DOI":"10.1016\/j.isci.2022.104542"},{"key":"9","doi-asserted-by":"crossref","unstructured":"9) Sato T, Vries RG, Snippert HJ, van de Wetering M, Barker N, Stange DE, van Es JH, Abo A, Kujala P, Peters PJ, Clevers H. Single Lgr5 stem cells build crypt-villus structures <i>in vitro<\/i> without a mesenchymal niche. <i>Nature<\/i>, <b>459<\/b>, 262\u2013265 (2009).","DOI":"10.1038\/nature07935"},{"key":"10","doi-asserted-by":"crossref","unstructured":"10) Koseki C, Ishikawa T, Sato Y, Shimada M, Yokoi Y, Nakamura K, Honma N, Moriyama T, Kashiwagi H, Sugawara M. Development of an evaluation system using intestinal organoids for drug efflux transport analysis by an imaging approach. <i>J. Pharm. Sci.<\/i>, <b>113<\/b>, 2675\u20132682 (2024).","DOI":"10.1016\/j.xphs.2024.06.007"},{"key":"11","doi-asserted-by":"crossref","unstructured":"11) In JG, Foulke-Abel J, Clarke E, Kovbasnjuk O. Human colonoid monolayers to study interactions between pathogens, commensals, and host intestinal epithelium. <i>J. Vis. Exp.<\/i>, e59357 (2019).","DOI":"10.3791\/59357-v"},{"key":"12","doi-asserted-by":"crossref","unstructured":"12) Yamashita T, Inui T, Yokota J, Kawakami K, Morinaga G, Takatani M, Hirayama D, Nomoto R, Ito K, Cui Y, Ruez S, Harada K, Kishimoto W, Nakase H, Mizuguchi H. Monolayer platform using human biopsy-derived duodenal organoids for pharmaceutical research. <i>Mol. Ther. Methods Clin. Dev.<\/i>, <b>22<\/b>, 263\u2013278 (2021).","DOI":"10.1016\/j.omtm.2021.05.005"},{"key":"13","doi-asserted-by":"crossref","unstructured":"13) Kramer N, Pratscher B, Meneses AMC, Tschulenk W, Walter I, Swoboda A, Kruitwagen HS, Schneeberger K, Penning LC, Spee B, Kieslinger M, Brandt S, Burgener IA. Generation of differentiating and long-living intestinal organoids reflecting the cellular diversity of canine intestine. <i>Cells<\/i>, <b>9<\/b>, 822 (2020).","DOI":"10.3390\/cells9040822"},{"key":"14","unstructured":"14) &lt;https:\/\/www.veritastk.co.jp\/sciencelibrary\/learning\/organnoid-2D-CaCO2.html&gt;, accessed 8 October, 2024."},{"key":"15","unstructured":"15) &lt;https:\/\/www.stemcell.com\/how-to-generate-mouse-intestinal-organoid-derived-monolayers-using-intesticult.html&gt;, accessed 8 October, 2024."},{"key":"16","doi-asserted-by":"crossref","unstructured":"16) Michiba K, Maeda K, Shimomura O, Miyazaki Y, Hashimoto S, Oda T, Kusuhara H. Usefulness of human jejunal spheroid-derived differentiated intestinal epithelial cells for the prediction of intestinal drug absorption in humans. <i>Drug Metab. Dispos.<\/i>, <b>50<\/b>, 204\u2013213 (2022).","DOI":"10.1124\/dmd.121.000796"},{"key":"17","doi-asserted-by":"crossref","unstructured":"17) Sato T, Stange DE, Ferrante M, Vries RGJ, Van Es JH, Van den Brink S, Van Houdt WJ, Pronk A, Van Gorp J, Siersema PD, Clevers H. Long-term expansion of epithelial organoids from human colon, adenoma, adenocarcinoma, and Barrett\u2019s epithelium. <i>Gastroenterology<\/i>, <b>141<\/b>, 1762\u20131772 (2011).","DOI":"10.1053\/j.gastro.2011.07.050"},{"key":"18","doi-asserted-by":"crossref","unstructured":"18) Kozuka K, He Y, Koo-McCoy S, Kumaraswamy P, Nie B, Shaw K, Chan P, Leadbetter M, He L, Lewis JG, Zhong Z, Charmot D, Balaa M, King AJ, Caldwell JS, Siegel M. Development and characterization of a human and mouse intestinal epithelial cell monolayer platform. <i>Stem Cell Reports<\/i>, <b>9<\/b>, 1976\u20131990 (2017).","DOI":"10.1016\/j.stemcr.2017.10.013"},{"key":"19","doi-asserted-by":"crossref","unstructured":"19) Stroulios G, Stahl M, Elstone F, Chang W, Louis S, Eaves A, Simmini S, Conder RK. Culture methods to study apical-specific interactions using intestinal organoid models. <i>J. Vis. Exp.<\/i>, e62330 (2021).","DOI":"10.3791\/62330"},{"key":"20","doi-asserted-by":"crossref","unstructured":"20) Altay G, Larra\u00f1aga E, Tosi S, Barriga FM, Batlle E, Fern\u00e1ndez-Majada V, Mart\u00ednez E. Self-organized intestinal epithelial monolayers in crypt and villus-like domains show effective barrier function. <i>Sci. Rep.<\/i>, <b>9<\/b>, 10140 (2019).","DOI":"10.1038\/s41598-019-46497-x"},{"key":"21","doi-asserted-by":"crossref","unstructured":"21) Fan J, Liu S, Du Y, Morrison J, Shipman R, Pang KS. Up-regulation of transporters and enzymes by the vitamin D receptor ligands, 1alpha, 25-dihydroxyvitamin D<sub>3<\/sub> and vitamin D analogs, in the Caco-2 cell monolayer. <i>J. Pharmacol. Exp. Ther.<\/i>, <b>330<\/b>, 389\u2013402 (2009).","DOI":"10.1124\/jpet.108.149815"},{"key":"22","doi-asserted-by":"crossref","unstructured":"22) Wang K, Chen S, Xie W, Wan YJY. Retinoids induce cytochrome P450 3A4 through RXR\/VDR-mediated pathway. <i>Biochem. Pharmacol.<\/i>, <b>75<\/b>, 2204\u20132213 (2008).","DOI":"10.1016\/j.bcp.2008.02.030"},{"key":"23","doi-asserted-by":"crossref","unstructured":"23) Tsukimoto M, Ohashi R, Torimoto N, Togo Y, Suzuki T, Maeda T, Kagawa Y. Effects of the inhibition of intestinal P-glycoprotein on aliskiren pharmacokinetics in cynomolgus monkeys. <i>Biopharm. Drug Dispos.<\/i>, <b>36<\/b>, 15\u201333 (2015).","DOI":"10.1002\/bdd.1920"},{"key":"24","doi-asserted-by":"crossref","unstructured":"24) Jappar D, Wu SP, Hu Y, Smith DE. Significance and regional dependency of peptide transporter (PEPT) 1 in the intestinal permeability of glycylsarcosine: <i>in situ<\/i> single-pass perfusion studies in wild-type and PepT1 knockout mice. <i>Drug Metab. Dispos.<\/i>, <b>38<\/b>, 1740\u20131746 (2010).","DOI":"10.1124\/dmd.110.034025"},{"key":"25","doi-asserted-by":"crossref","unstructured":"25) Yano K, Tomono T, Sakai R, Kano T, Morimoto K, Kato Y, Ogihara T. Contribution of radixin to P-glycoprotein expression and transport activity in mouse small intestine <i>in vivo<\/i>. <i>J. Pharm. Sci.<\/i>, <b>102<\/b>, 2875\u20132881 (2013).","DOI":"10.1002\/jps.23637"},{"key":"26","doi-asserted-by":"crossref","unstructured":"26) Renaud HJ, Cui JY, Khan M, Klaassen CD. Tissue distribution and gender-divergent expression of 78 cytochrome P450 mRNAs in mice. <i>Toxicol. Sci.<\/i>, <b>124<\/b>, 261\u2013277 (2011).","DOI":"10.1093\/toxsci\/kfr240"},{"key":"27","doi-asserted-by":"crossref","unstructured":"27) Inui T, Uraya Y, Yokota J, Yamashita T, Kawai K, Okada K, Ueyama-Toba Y, Mizuguchi H. Functional intestinal monolayers from organoids derived from human iPS cells for drug discovery research. <i>Stem Cell Res. Ther.<\/i>, <b>15<\/b>, 57 (2024).","DOI":"10.1186\/s13287-024-03685-5"},{"key":"28","doi-asserted-by":"crossref","unstructured":"28) Kalb S, Martirosyan NL, Kalani MYS, Broc GG, Theodore N. Genetics of the degenerated intervertebral disc. <i>World Neurosurg.<\/i>, <b>77<\/b>, 491\u2013501 (2012).","DOI":"10.1016\/j.wneu.2011.07.014"},{"key":"29","doi-asserted-by":"crossref","unstructured":"29) Gr\u00f6ber U, Spitz J, Reichrath J, Kisters K, Holick MF. Vitamin D: update 2013: from rickets prophylaxis to general preventive healthcare. <i>Dermatoendocrinol<\/i>, <b>5<\/b>, 331\u2013347 (2013).","DOI":"10.4161\/derm.26738"},{"key":"30","doi-asserted-by":"crossref","unstructured":"30) Durk MR, Chan GNY, Campos CR, Peart JC, Chow ECY, Lee E, Cannon RE, Bendayan R, Miller DS, Pang KS. 1<i>\u03b1<\/i>,25-Dihydroxyvitamin D3-liganded vitamin D receptor increases expression and transport activity of P-glycoprotein in isolated rat brain capillaries and human and rat brain microvessel endothelial cells. <i>J. 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