{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T18:14:47Z","timestamp":1785176087902,"version":"3.55.0"},"reference-count":67,"publisher":"Pharmaceutical Society of Japan","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Chem. Pharm. Bull.","CHEMICAL & PHARMACEUTICAL BULLETIN"],"published-print":{"date-parts":[[2026,1,1]]},"DOI":"10.1248\/cpb.c25-00652","type":"journal-article","created":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T22:09:16Z","timestamp":1767218956000},"page":"1-15","source":"Crossref","is-referenced-by-count":1,"title":["Structure-Guided Engineering of 2-Oxoglutarate-Dependent Oxygenases: Principles, Case Studies, and Emerging Opportunities"],"prefix":"10.1248","volume":"74","author":[{"given":"Yu","family":"Nakashima","sequence":"first","affiliation":[{"name":"Institute of Natural Medicine, University of Toyama"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"163","reference":[{"key":"1","unstructured":"1) Hausinger R. P., Crit. Rev. Biochem. Mol. Biol.<\/i>, 39<\/b>, 21\u201368 (2004)."},{"key":"2","doi-asserted-by":"crossref","unstructured":"2) Koehntop K. D., Emerson J. P., Que L. Jr., J. Biol. Inorg. Chem.<\/i>, 10<\/b>, 87\u201393 (2005).","DOI":"10.1007\/s00775-005-0624-x"},{"key":"3","doi-asserted-by":"crossref","unstructured":"3) Clifton I. J., McDonough M. A., Ehrismann D., Kershaw N. J., Granatino N., Schofield C. J., J. Inorg. Biochem.<\/i>, 100<\/b>, 644\u2013669 (2006).","DOI":"10.1016\/j.jinorgbio.2006.01.024"},{"key":"4","doi-asserted-by":"crossref","unstructured":"4) Vaillancourt F. H., Yeh E., Vosburg D. A., Garneau-Tsodikova S., Walsh C. T., Chem. Rev.<\/i>, 106<\/b>, 3364\u20133378 (2006).","DOI":"10.1021\/cr050313i"},{"key":"5","doi-asserted-by":"crossref","unstructured":"5) Martinez S., Hausinger R. P., J. Biol. Chem.<\/i>, 290<\/b>, 20702\u201320711 (2015).","DOI":"10.1074\/jbc.R115.648691"},{"key":"6","doi-asserted-by":"crossref","unstructured":"6) Price J. C., Barr E. W., Glass T. E., Krebs C., Bollinger J. M. Jr., J. Am. Chem. Soc.<\/i>, 125<\/b>, 13008\u201313009 (2003).","DOI":"10.1021\/ja037400h"},{"key":"7","doi-asserted-by":"crossref","unstructured":"7) Hoffart L. M., Barr E. W., Guyer R. B., Bollinger J. M. Jr., Krebs C., Proc. Natl. Acad. Sci. U.S.A.<\/i>, 103<\/b>, 14738\u201314743 (2006).","DOI":"10.1073\/pnas.0604005103"},{"key":"8","doi-asserted-by":"crossref","unstructured":"8) Loenarz C., Schofield C. J., Nat. Chem. Biol.<\/i>, 4<\/b>, 152\u2013156 (2008).","DOI":"10.1038\/nchembio0308-152"},{"key":"9","doi-asserted-by":"crossref","unstructured":"9) McDonough M. A., Loenarz C., Chowdhury R., Clifton I. J., Schofield C. J., Curr. Opin. Struct. Biol.<\/i>, 20<\/b>, 659\u2013672 (2010).","DOI":"10.1016\/j.sbi.2010.08.006"},{"key":"10","doi-asserted-by":"crossref","unstructured":"10) Islam M. S., Leissing T. M., Chowdhury R., Hopkinson R. J., Schofield C. J., Annu. Rev. Biochem.<\/i>, 87<\/b>, 585\u2013620 (2018).","DOI":"10.1146\/annurev-biochem-061516-044724"},{"key":"11","doi-asserted-by":"crossref","unstructured":"11) Matsuda Y., Abe I., Nat. Prod. Rep.<\/i>, 33<\/b>, 26\u201353 (2016).","DOI":"10.1039\/C5NP00090D"},{"key":"12","doi-asserted-by":"crossref","unstructured":"12) Matsuda Y., Awakawa T., Mori T., Abe I., Curr. Opin. Chem. Biol.<\/i>, 31<\/b>, 1\u20137 (2016).","DOI":"10.1016\/j.cbpa.2015.11.001"},{"key":"13","doi-asserted-by":"crossref","unstructured":"13) Gao S. S., Naowarojna N., Cheng R., Liu X., Liu P., Nat. Prod. Rep.<\/i>, 35<\/b>, 792\u2013837 (2018).","DOI":"10.1039\/C7NP00067G"},{"key":"14","doi-asserted-by":"crossref","unstructured":"14) Herr C. Q., Hausinger R. P., Trends Biochem. Sci.<\/i>, 43<\/b>, 517\u2013532 (2018).","DOI":"10.1016\/j.tibs.2018.04.002"},{"key":"15","doi-asserted-by":"crossref","unstructured":"15) Nakamura H., Matsuda Y., Abe I., Nat. Prod. Rep.<\/i>, 35<\/b>, 633\u2013645 (2018).","DOI":"10.1039\/C7NP00055C"},{"key":"16","doi-asserted-by":"crossref","unstructured":"16) Awakawa T., J. Nat. Med.<\/i>, 79<\/b>, 303\u2013313 (2025).","DOI":"10.1007\/s11418-025-01880-z"},{"key":"17","doi-asserted-by":"crossref","unstructured":"17) Shen Y., Sun A., Guo Y., Chang W. C., ACS Bio. Med. Chem. Au.<\/i>, 5<\/b>, 238\u2013261 (2025).","DOI":"10.1021\/acsbiomedchemau.5c00001"},{"key":"18","doi-asserted-by":"crossref","unstructured":"18) Peters C., Buller R. M., Catalysts<\/i>, 9<\/b>, 221 (2019).","DOI":"10.3390\/catal9030221"},{"key":"19","doi-asserted-by":"crossref","unstructured":"19) Tao H., Abe I., Curr. Opin. Biotechnol.<\/i>, 77<\/b>, 102763 (2022).","DOI":"10.1016\/j.copbio.2022.102763"},{"key":"20","doi-asserted-by":"crossref","unstructured":"20) Zhao S., Wu L., Xu Y., Nie Y., Nat. Prod. Rep.<\/i>, 42<\/b>, 67\u201392 (2025).","DOI":"10.1039\/D4NP00030G"},{"key":"21","doi-asserted-by":"crossref","unstructured":"21) Awakawa T., Mori T., Ushimaru R., Abe I., Nat. Prod. Rep.<\/i>, 40<\/b>, 46\u201361 (2023).","DOI":"10.1039\/D2NP00014H"},{"key":"22","doi-asserted-by":"crossref","unstructured":"22) Markolovic S., Leissing T. M., Chowdhury R., Wilkins S. E., Lu X., Schofield C. J., Curr. Opin. Struct. Biol.<\/i>, 41<\/b>, 62\u201372 (2016).","DOI":"10.1016\/j.sbi.2016.05.013"},{"key":"23","doi-asserted-by":"crossref","unstructured":"23) Pfeffer I., Brewitz L., Krojer T., Jensen S. A., Kochan G. T., Kershaw N. J., Hewitson K. S., McNeill L. A., Kramer H., M\u00fcnzel M., Hopkinson R. J., Oppermann U., Handford P. A., McDonough M. A., Schofield C. J., Nat. Commun.<\/i>, 10<\/b>, 4910 (2019).","DOI":"10.1038\/s41467-019-12711-7"},{"key":"24","doi-asserted-by":"crossref","unstructured":"24) Yang S., Xing J., Liu D., Song Y., Yu H., Xu S., Zuo Y., Int. J. Biol. Macromol.<\/i>, 278<\/b>, 134798 (2024).","DOI":"10.1016\/j.ijbiomac.2024.134798"},{"key":"25","doi-asserted-by":"crossref","unstructured":"25) Blasiak L. C., Vaillancourt F. H., Walsh C. T., Drennan C. L., Nature<\/i>, 440<\/b>, 368\u2013371 (2006).","DOI":"10.1038\/nature04544"},{"key":"26","doi-asserted-by":"crossref","unstructured":"26) Crowe C., Molyneux S., Sharma S. V., Zhang Y., Gkotsi D. S., Connaris H., Goss R. J. M., Chem. Soc. Rev.<\/i>, 50<\/b>, 9443\u20139481 (2021).","DOI":"10.1039\/D0CS01551B"},{"key":"27","doi-asserted-by":"crossref","unstructured":"27) Voss M., Honda Malca S., Buller R., Chem. Eur. J.<\/i>, 26<\/b>, 7336\u20137345 (2020).","DOI":"10.1002\/chem.201905752"},{"key":"28","doi-asserted-by":"crossref","unstructured":"28) Papadopoulou A., Meyer F., Buller R. M., Biochemistry<\/i>, 62<\/b>, 229\u2013240 (2023).","DOI":"10.1021\/acs.biochem.2c00115"},{"key":"29","doi-asserted-by":"crossref","unstructured":"29) Hibi M., Kawashima T., Kasahara T., Sokolov P. M., Smirnov S. V., Kodera T., Sugiyama M., Shimizu S., Yokozeki K., Ogawa J., Lett. Appl. Microbiol.<\/i>, 55<\/b>, 414\u2013419 (2012).","DOI":"10.1111\/j.1472-765X.2012.03308.x"},{"key":"30","doi-asserted-by":"crossref","unstructured":"30) Qin H. M., Miyakawa T., Jia M.-Z., Nakamura A., Ohtsuka J., Xue Y. L., Kawashima T., Kasahara T., Hibi M., Ogawa J., Tanokura M., PLOS ONE<\/i>, 8<\/b>, e63996 (2013).","DOI":"10.1371\/journal.pone.0063996"},{"key":"31","doi-asserted-by":"crossref","unstructured":"31) Mitchell A. J., Zhu Q., Maggiolo A. O., Ananth N. R., Hillwig M. L., Liu X., Boal A. K., Nat. Chem. Biol.<\/i>, 12<\/b>, 636\u2013640 (2016).","DOI":"10.1038\/nchembio.2112"},{"key":"32","doi-asserted-by":"crossref","unstructured":"32) Mitchell A. J., Dunham N. P., Bergman J. A., Wang B., Zhu Q., Chang W.-C., Liu X., Boal A. K., Biochemistry<\/i>, 56<\/b>, 441\u2013444 (2017).","DOI":"10.1021\/acs.biochem.6b01173"},{"key":"33","doi-asserted-by":"crossref","unstructured":"33) Gomez C. A., Mondal D., Du Q., Chan N., Lewis J. C., Angew. Chem. Int. Ed.<\/i>, 62<\/b>, e202301370 (2023).","DOI":"10.1002\/anie.202301370"},{"key":"34","doi-asserted-by":"crossref","unstructured":"34) Hara R., Kino K., Biochem. Biophys. Res. Commun.<\/i>, 379<\/b>, 882\u2013886 (2009).","DOI":"10.1016\/j.bbrc.2008.12.158"},{"key":"35","doi-asserted-by":"crossref","unstructured":"35) Papadopoulou A., Meierhofer J., Meyer F., Hayashi T., Schneider S., Sager E., Buller R., ChemCatChem<\/i>, 13<\/b>, 3914\u20133919 (2021).","DOI":"10.1002\/cctc.202100591"},{"key":"36","doi-asserted-by":"crossref","unstructured":"36) Hillwig M. L., Liu X., Nat. Chem. Biol.<\/i>, 10<\/b>, 921\u2013923 (2014).","DOI":"10.1038\/nchembio.1625"},{"key":"37","doi-asserted-by":"crossref","unstructured":"37) Hillwig M. L., Fuhrman H. A., Ittiamornkul K., Sevco T. J., Kwak D. H., Liu X., ChemBioChem<\/i>, 15<\/b>, 665\u2013669 (2014).","DOI":"10.1002\/cbic.201300794"},{"key":"38","doi-asserted-by":"crossref","unstructured":"38) Zhu Q., Liu X., Beilstein J. Org. Chem.<\/i>, 13<\/b>, 1168\u20131173 (2017).","DOI":"10.3762\/bjoc.13.115"},{"key":"39","doi-asserted-by":"crossref","unstructured":"39) Hayashi T., Ligibel M., Sager E., Voss M., Hunziker J., Schroer K., Snajdrova R., Buller R., Angew. Chem. Int. Ed.<\/i>, 58<\/b>, 18535\u201318539 (2019).","DOI":"10.1002\/anie.201907245"},{"key":"40","doi-asserted-by":"crossref","unstructured":"40) Voss M., H\u00fcppi S., Schaub D., Hayashi T., Ligibel M., Sager E., Schroer K., Snajdrova R., Buller R., ChemCatChem<\/i>, 14<\/b>, e202201115 (2022).","DOI":"10.1002\/cctc.202201115"},{"key":"41","doi-asserted-by":"crossref","unstructured":"41) B\u00fcchler J., Malca S. H., Patsch D., Voss M., Turner N. J., Bornscheuer U. T., Allemann O., Le Chapelain C., Lumbroso A., Loiseleur O., Buller R., Nat. Commun.<\/i>, 13<\/b>, 371 (2022).","DOI":"10.1038\/s41467-022-27999-1"},{"key":"42","doi-asserted-by":"crossref","unstructured":"42) Duewel S., Schmermund L., Faber T., Harms K., Srinivasan V., Meggers E., Hoebenreich S., ACS Catal.<\/i>, 10<\/b>, 1272\u20131277 (2020).","DOI":"10.1021\/acscatal.9b04691"},{"key":"43","doi-asserted-by":"crossref","unstructured":"43) Matthews M. L., Chang W. C., Layne A. P., Miles L. A., Krebs C., Bollinger J. M. Jr., Nat. Chem. Biol.<\/i>, 10<\/b>, 209\u2013215 (2014).","DOI":"10.1038\/nchembio.1438"},{"key":"44","doi-asserted-by":"crossref","unstructured":"44) Neugebauer M. E., Sumida K. H., Pelton J. G., McMurry J. L., Marchand J. A., Chang M. C. Y., Nat. Chem. Biol.<\/i>, 15<\/b>, 1009\u20131016 (2019).","DOI":"10.1038\/s41589-019-0355-x"},{"key":"45","doi-asserted-by":"crossref","unstructured":"45) Quan Z., Awakawa T., Beilstein J. Org. Chem.<\/i>, 20<\/b>, 578\u2013588 (2024).","DOI":"10.3762\/bjoc.20.50"},{"key":"46","doi-asserted-by":"crossref","unstructured":"46) Lo H. C., Entwistle R., Guo C. J., Ahuja M., Szewczyk E., Hung J. H., Chiang Y. M., Oakley B. R., Wang C. C., J. Am. Chem. Soc.<\/i>, 134<\/b>, 4709\u20134720 (2012).","DOI":"10.1021\/ja209809t"},{"key":"47","doi-asserted-by":"crossref","unstructured":"47) Matsuda Y., Iwabuchi T., Fujimoto T., Awakawa T., Nakashima Y., Mori T., Zhang H., Hayashi F., Abe I., J. Am. Chem. Soc.<\/i>, 138<\/b>, 12671\u201312677 (2016).","DOI":"10.1021\/jacs.6b08424"},{"key":"48","doi-asserted-by":"crossref","unstructured":"48) Matsuda Y., Awakawa T., Wakimoto T., Abe I., J. Am. Chem. Soc.<\/i>, 135<\/b>, 10962\u201310965 (2013).","DOI":"10.1021\/ja405518u"},{"key":"49","doi-asserted-by":"crossref","unstructured":"49) Nakashima Y., Mori T., Nakamura H., Awakawa T., Hoshino S., Senda M., Senda T., Abe I., Nat. Commun.<\/i>, 9<\/b>, 104 (2018).","DOI":"10.1038\/s41467-017-02371-w"},{"key":"50","doi-asserted-by":"crossref","unstructured":"50) Matsuda Y., Wakimoto T., Mori T., Awakawa T., Abe I., J. Am. Chem. Soc.<\/i>, 136<\/b>, 15326\u201315336 (2014).","DOI":"10.1021\/ja508127q"},{"key":"51","doi-asserted-by":"crossref","unstructured":"51) Nakashima Y., Mitsuhashi T., Matsuda Y., Senda M., Sato H., Yamazaki M., Uchiyama M., Senda T., Abe I., J. Am. Chem. Soc.<\/i>, 140<\/b>, 9743\u20139750 (2018).","DOI":"10.1021\/jacs.8b06084"},{"key":"52","doi-asserted-by":"crossref","unstructured":"52) Mori T., Nakashima Y., Chen H., Hoshino S., Mitsuhashi T., Abe I., Chem. Commun.<\/i>, 58<\/b>, 5510\u20135513 (2022).","DOI":"10.1039\/D2CC00736C"},{"key":"53","doi-asserted-by":"crossref","unstructured":"53) Bai T., Matsuda Y., Tao H., Mori T., Zhang Y., Abe I., Org. Lett.<\/i>, 22<\/b>, 4311\u20134315 (2020).","DOI":"10.1021\/acs.orglett.0c01358"},{"key":"54","doi-asserted-by":"crossref","unstructured":"54) Tao H., Mori T., Chen H., Lyu S., Nonoyama A., Lee S., Abe I., Nat. Commun.<\/i>, 13<\/b>, 95 (2022).","DOI":"10.1038\/s41467-021-27636-3"},{"key":"55","doi-asserted-by":"crossref","unstructured":"55) Steffan N., Grundmann A., Afiyatullov S., Ruan H., Li S. M., Org. Biomol. Chem.<\/i>, 7<\/b>, 4082\u20134087 (2009).","DOI":"10.1039\/b908392h"},{"key":"56","doi-asserted-by":"crossref","unstructured":"56) Zhu G., Yan W., Wang X., Cheng R., Naowarojna N., Wang K., Wang J., Song H., Wang Y., Liu H., Xia X., Costello C. E., Liu X., Zhang L., Liu P., JACS Au.<\/i>, 2<\/b>, 1686\u20131698 (2022).","DOI":"10.1021\/jacsau.2c00248"},{"key":"57","doi-asserted-by":"crossref","unstructured":"57) Wu L., Wang Z., Cen Y., Wang B., Zhou J., Angew. Chem. Int. Ed.<\/i>, 61<\/b>, e202112063 (2022).","DOI":"10.1002\/anie.202205382"},{"key":"58","doi-asserted-by":"crossref","unstructured":"58) Wang F., Gao Y., Wang C., Lan W., Gan J., Cao C., Catal. Sci. Technol.<\/i>, 13<\/b>, 6839\u20136849 (2023).","DOI":"10.1039\/D3CY00092C"},{"key":"59","doi-asserted-by":"crossref","unstructured":"59) Wang X., Yang L., Wang S., Wang J., Li K., Naowarojna N., Ju Y., Ye K., Han Y., Yan W., Liu X., Zhang L., Liu P., Catal. Sci. Technol.<\/i>, 15<\/b>, 386\u2013395 (2025).","DOI":"10.1039\/D4CY01077A"},{"key":"60","doi-asserted-by":"crossref","unstructured":"60) Matsuda Y., Bai T., Phippen C. B. W., N\u00f8dvig C. S., Kj\u00e6rb\u00f8lling I., Vesth T. C., Andersen M. R., Mortensen U. H., Gotfredsen C. H., Abe I., Larsen T. O., Nat. Commun.<\/i>, 9<\/b>, 2587 (2018).","DOI":"10.1038\/s41467-018-04983-2"},{"key":"61","doi-asserted-by":"crossref","unstructured":"61) Mori T., Zhai R., Ushimaru R., Matsuda Y., Abe I., Nat. Commun.<\/i>, 12<\/b>, 4417 (2021).","DOI":"10.1038\/s41467-021-24685-6"},{"key":"62","doi-asserted-by":"crossref","unstructured":"62) Tao H., Abe I., Eng. Microbiol.<\/i>, 3<\/b>, 100062 (2023).","DOI":"10.1016\/j.engmic.2022.100062"},{"key":"63","doi-asserted-by":"crossref","unstructured":"63) Bunno R., Awakawa T., Mori T., Abe I., Angew. Chem. Int. Ed.<\/i>, 60<\/b>, 15827\u201315831 (2021).","DOI":"10.1002\/anie.202104644"},{"key":"64","doi-asserted-by":"crossref","unstructured":"64) Tao H., Ushimaru R., Awakawa T., Mori T., Uchiyama M., Abe I., J. Am. Chem. Soc.<\/i>, 144<\/b>, 21512\u201321520 (2022).","DOI":"10.1021\/jacs.2c08116"},{"key":"65","doi-asserted-by":"crossref","unstructured":"65) Meyer F., Frey R., Ligibel M., Sager E., Schroer K., Snajdrova R., Buller R., ACS Catal.<\/i>, 11<\/b>, 6261\u20136269 (2021).","DOI":"10.1021\/acscatal.1c00678"},{"key":"66","doi-asserted-by":"crossref","unstructured":"66) Koketsu K., Shomura Y., Moriwaki K., Hayashi M., Mitsuhashi S., Hara R., Kino K., Higuchi Y., ACS Synth. Biol.<\/i>, 4<\/b>, 383\u2013392 (2015).","DOI":"10.1021\/sb500247a"},{"key":"67","doi-asserted-by":"crossref","unstructured":"67) Abramson J., Adler J., Dunger J., et al.<\/i>, Nature<\/i>, 630<\/b>, 493\u2013500 (2024).","DOI":"10.1038\/s41586-024-07487-w"}],"container-title":["Chemical and Pharmaceutical Bulletin"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jstage.jst.go.jp\/article\/cpb\/74\/1\/74_c25-00652\/_pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,3]],"date-time":"2026-01-03T03:26:49Z","timestamp":1767410809000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jstage.jst.go.jp\/article\/cpb\/74\/1\/74_c25-00652\/_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1,1]]},"references-count":67,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026]]}},"URL":"https:\/\/doi.org\/10.1248\/cpb.c25-00652","relation":{},"ISSN":["0009-2363","1347-5223"],"issn-type":[{"value":"0009-2363","type":"print"},{"value":"1347-5223","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1,1]]},"article-number":"c25-00652"}}