{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T21:49:48Z","timestamp":1777585788595,"version":"3.51.4"},"reference-count":12,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2005,4,1]],"date-time":"2005-04-01T00:00:00Z","timestamp":1112313600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Mass Spectrom (Chichester)"],"published-print":{"date-parts":[[2005,4]]},"abstract":"<jats:p>\n                    An on-line liquid chromatography\/tandem mass spectrometry (LC-MS\/MS) procedure, using the Prospekt-2 system, was developed and used for the determination of the levels of the active ingredients of cough\/cold medications in human plasma matrix. The experimental configuration allows direct plasma injection by performing on-line solid phase extraction (SPE) on small cartridge columns prior to elution of the analyte(s) onto the analytical column and subsequent MS\/MS detection. The quantitative analysis of three analytes with differing polarities, dextromethorphan (DEX), dextrorphan (DET) and guaifenesin (GG) in human plasma presented a significant challenge. Using stable-isotope-labeled internal standards for each analyte, the Prospekt-2 on-line methodology was evaluated for sensitivity, suppression, accuracy, precision, linearity, analyst time, analysis time, cost, carryover and ease of use. The lower limit of quantitation for the on-line SPE procedure for DEX, DET and GG was 0.05, 0.05 and 5.0 ng mL\n                    <jats:sup>\u22121<\/jats:sup>\n                    , respectively, using a 0.1 mL sample volume. The linear range for DEX and DET was 0.05\u201350 ng mL\n                    <jats:sup>\u22121<\/jats:sup>\n                    and was 5\u20135,000 ng mL\n                    <jats:sup>\u22121<\/jats:sup>\n                    for GG. Accuracy and precision data for five different levels of QC samples were collected over three separate days. Accuracy ranged from 90% to 112% for all three analytes, while the precision, as measured by the %RSD, ranged from 1.5% to 16.0%.\n                  <\/jats:p>","DOI":"10.1255\/ejms.737","type":"journal-article","created":{"date-parts":[[2005,7,27]],"date-time":"2005-07-27T04:41:35Z","timestamp":1122439295000},"page":"199-208","source":"Crossref","is-referenced-by-count":16,"title":["On-Line Solid Phase Extraction Using the Prospekt-2 Coupled with a Liquid Chromatography\/Tandem Mass Spectrometer for the Determination of Dextromethorphan, Dextrorphan and Guaifenesin in Human Plasma"],"prefix":"10.1177","volume":"11","author":[{"given":"Debbie L.","family":"Kuhlenbeck","sequence":"first","affiliation":[{"name":"Procter & Gamble Pharmaceuticals, Health Care Research Center, 8700 Mason-Montgomery Rd, Mason, OH 45040-8006, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas H.","family":"Eichhold","sequence":"additional","affiliation":[{"name":"The Procter & Gamble Company, Health Care Research Center, 8700 Mason-Montgomery Rd, Mason, OH 45040-8006, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"II","given":"Steven H.","family":"Hoke","sequence":"additional","affiliation":[{"name":"The Procter & Gamble Company, Health Care Research Center, 8700 Mason-Montgomery Rd, Mason, OH 45040-8006, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Timothy R.","family":"Baker","sequence":"additional","affiliation":[{"name":"Procter & Gamble Pharmaceuticals, Health Care Research Center, 8700 Mason-Montgomery Rd, Mason, OH 45040-8006, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert","family":"Mensen","sequence":"additional","affiliation":[{"name":"Spark Holland Inc., 666 Plainsboro Rd, Plainsboro, NJ 08536, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenneth R.","family":"Wehmeyer","sequence":"additional","affiliation":[{"name":"Procter & Gamble Pharmaceuticals, Health Care Research Center, 8700 Mason-Montgomery Rd, Mason, OH 45040-8006, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2005,4,1]]},"reference":[{"key":"bibr1-ejms.737","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(97)00308-3"},{"key":"bibr2-ejms.737","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9888(199711)32:11<1205::AID-JMS579>3.0.CO;2-C"},{"key":"bibr3-ejms.737","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0378-4347(01)00555-2","volume":"772","author":"Bolden R.D.","year":"2002","journal-title":"J. Chromatogr. B: Biomed. Sci. Appl."},{"key":"bibr4-ejms.737","doi-asserted-by":"publisher","DOI":"10.1016\/S0731-7085(00)00346-0"},{"key":"bibr5-ejms.737","doi-asserted-by":"publisher","DOI":"10.1016\/0731-7085(90)80020-P"},{"key":"bibr6-ejms.737","unstructured":"Applications Note, Liquid\/Liquid Extraction with the MICROLAB ATplus 2 Automated Workstation, Lit. No. RR-0306-01,2003. http:\/\/www.hamiltoncompany.com\/product\/robotics\/liquid.html"},{"key":"bibr7-ejms.737","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(99)00535-X"},{"key":"bibr8-ejms.737","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(03)00773-8"},{"key":"bibr9-ejms.737","doi-asserted-by":"publisher","DOI":"10.1016\/S0731-7085(96)02011-0"},{"key":"bibr10-ejms.737","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9888(1998110)33:11<1071::AID-JMS719>3.0.CO;2-B"},{"key":"bibr11-ejms.737","doi-asserted-by":"publisher","DOI":"10.1080\/10826079608006291"},{"key":"bibr12-ejms.737","doi-asserted-by":"publisher","DOI":"10.1002\/jlcr.640"}],"container-title":["European Journal of Mass Spectrometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1255\/ejms.737","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1255\/ejms.737","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T20:02:04Z","timestamp":1777406524000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1255\/ejms.737"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,4]]},"references-count":12,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2005,4]]}},"alternative-id":["10.1255\/ejms.737"],"URL":"https:\/\/doi.org\/10.1255\/ejms.737","relation":{},"ISSN":["1469-0667","1751-6838"],"issn-type":[{"value":"1469-0667","type":"print"},{"value":"1751-6838","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,4]]}}}