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Chemical cross-linking combined with Fourier transform ion cyclotron resonance mass spectrometry has been employed to investigate the coordination of calmodulin and melittin in the complex at different concentrations of calcium. This methodology can be used to monitor structural changes in proteins induced by ligand binding and to study the effects these changes have on non-covalent interactions between proteins. Cross-linking results indicate that the binding place of the first melittin in the calcium-free calmodulin form is the same as in the calcium-loaded calmodulin\/melittin complex.<\/jats:p>","DOI":"10.1255\/ejms.882","type":"journal-article","created":{"date-parts":[[2007,10,17]],"date-time":"2007-10-17T11:28:59Z","timestamp":1192620539000},"page":"281-290","source":"Crossref","is-referenced-by-count":11,"title":["Monitoring Conformational Changes in Protein Complexes Using Chemical Cross-Linking and Fourier Transform Ion Cyclotron Resonance Mass Spectrometry: The Effect of Calcium Binding on the Calmodulin\u2014Melittin Complex"],"prefix":"10.1177","volume":"13","author":[{"given":"Petr","family":"Novak","sequence":"first","affiliation":[{"name":"Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague 142 20, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vladimir","family":"Havlicek","sequence":"additional","affiliation":[{"name":"Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague 142 20, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter J.","family":"Derrick","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Warwick, Coventry, CV4 7AL, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyle A.","family":"Beran","sequence":"additional","affiliation":[{"name":"Department of Science & Mathematics, The University of Texas of the Permian Basin, Odessa, TX 79762, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sajid","family":"Bashir","sequence":"additional","affiliation":[{"name":"Chemical Biology Research Group, Department of Chemistry, Texas A&M-Kingsville, Kingsville, TX 78363, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anastassios E.","family":"Giannakopulos","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Warwick, Coventry, CV4 7AL, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2007,8,1]]},"reference":[{"key":"bibr1-ejms.882","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.46.070177.002515"},{"key":"bibr2-ejms.882","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.62.070193.002411"},{"key":"bibr3-ejms.882","doi-asserted-by":"publisher","DOI":"10.1016\/S1367-5931(99)00047-2"},{"key":"bibr4-ejms.882","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.090099097"},{"key":"bibr5-ejms.882","doi-asserted-by":"publisher","DOI":"10.1042\/bj2490621"},{"key":"bibr6-ejms.882","doi-asserted-by":"publisher","DOI":"10.1139\/o94-049"},{"key":"bibr7-ejms.882","doi-asserted-by":"publisher","DOI":"10.1038\/315037a0"},{"key":"bibr8-ejms.882","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(88)90608-0"},{"key":"bibr9-ejms.882","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(92)90324-D"},{"key":"bibr10-ejms.882","doi-asserted-by":"crossref","first-page":"12175","DOI":"10.1016\/S0021-9258(18)37733-0","volume":"263","author":"Persechini A.","year":"1988","journal-title":"J. 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